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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
02/10/2012 |
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Data da última atualização: |
25/02/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DOMINGUES, L. N.; BRASIL, B. dos S. A. F.; BELLO, A. C. P. de P.; CUNHA, A. P. da; BARROS, A. T. M. de; LEITE, R. C.; SILAGHI, C.; PFISTER, K.; PASSOS, L. M. F. |
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Afiliação: |
LUÍSA NOGUEIRA DOMINGUES, UFMG; BRUNO DOS SANTOS ALVES FIGUEIREDO BRASIL, UFMG; ANA CRISTINA PASSOS DE PAIVA BELLO, UFMG; ARILDO PINTO DA CUNHA, UFMG; ANTONIO THADEU MEDEIROS DE BARROS, CPAP; ROMÁRIO CERQUEIRA LEITE, UFMG; CORNELIA SILAGHI, UNIVERSITAT MUNCHEN; KURT PFISTER, UNIVERSITAT MUNCHEN; LYGIA MARIA FRICHE PASSOS, UFMG - UNIVERSITAT MUNCHEN. |
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Título: |
Survey of pyrethroid and organophosphate resistance in Brazilian field populations of Rhipicephalus (Boophilus) microplus: Detection of C190A mutation in domain II of the para-type sodium channel gene. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Veterinary Parasitology, v. 189, n.2-4, p. 327-332, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
The cattle tick Rhipicephalus (Boophilus) microplus causes expressive damage to livestock in Brazil and other countries. Its control is becoming more difficult due to the development of resistance in populations. Early detection of resistance can help in developing effective control strategies. This study evaluated the susceptibility of R. microplus to cypermethrin and chlorpyriphos and was the first attempt to identify the mechanism of resistance (target site insensitivity) in cattle tick populations from Minas Gerais state (Southeastern Brazil). Engorged female ticks were collected from 10 ranches within the state of Minas Gerais, and susceptibility was evaluated with the larval packet test (LPT) using technical grade cypermethrin and chlorpyriphos. It was possible to analyze LPT results of seven populations. Target site insensitivity was investigated in all 10 isolates by using molecular approaches for detection of the T2134A substitution within the domain III S6 segment and the C190A in the domain II S4-5 linker from the para-type sodium channel gene. LPT showed that all seven populations were resistant to cypermethrin with resistance ratio (RR) ranging from 16.0 to 25.0 and 85.7% were resistant to chlorpyriphos (RR = 2.2?15.6). Although the T2134A mutation was not detected, the C190A mutation was highly prevalent, being present in 82?100% of the alleles sampled in field populations. A significant correlation was found between the LC50 values for cypermethrin and the frequency of the C190A mutation suggesting that it might be responsible for the phenotypic resistance detected. MenosThe cattle tick Rhipicephalus (Boophilus) microplus causes expressive damage to livestock in Brazil and other countries. Its control is becoming more difficult due to the development of resistance in populations. Early detection of resistance can help in developing effective control strategies. This study evaluated the susceptibility of R. microplus to cypermethrin and chlorpyriphos and was the first attempt to identify the mechanism of resistance (target site insensitivity) in cattle tick populations from Minas Gerais state (Southeastern Brazil). Engorged female ticks were collected from 10 ranches within the state of Minas Gerais, and susceptibility was evaluated with the larval packet test (LPT) using technical grade cypermethrin and chlorpyriphos. It was possible to analyze LPT results of seven populations. Target site insensitivity was investigated in all 10 isolates by using molecular approaches for detection of the T2134A substitution within the domain III S6 segment and the C190A in the domain II S4-5 linker from the para-type sodium channel gene. LPT showed that all seven populations were resistant to cypermethrin with resistance ratio (RR) ranging from 16.0 to 25.0 and 85.7% were resistant to chlorpyriphos (RR = 2.2?15.6). Although the T2134A mutation was not detected, the C190A mutation was highly prevalent, being present in 82?100% of the alleles sampled in field populations. A significant correlation was found between the LC50 values for cypermethrin and the fre... Mostrar Tudo |
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Palavras-Chave: |
Allele-specific PCR; Cattle tick; Chlorpyriphos; Larval packet test; Target site insensitivity. |
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Thesaurus Nal: |
cypermethrin. |
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Categoria do assunto: |
O Insetos e Entomologia |
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Marc: |
LEADER 02556naa a2200289 a 4500
001 1935239
005 2013-02-25
008 2012 bl --- 0-- u #d
100 1 $aDOMINGUES, L. N.
245 $aSurvey of pyrethroid and organophosphate resistance in Brazilian field populations of Rhipicephalus (Boophilus) microplus$bDetection of C190A mutation in domain II of the para-type sodium channel gene.
260 $c2012
520 $aThe cattle tick Rhipicephalus (Boophilus) microplus causes expressive damage to livestock in Brazil and other countries. Its control is becoming more difficult due to the development of resistance in populations. Early detection of resistance can help in developing effective control strategies. This study evaluated the susceptibility of R. microplus to cypermethrin and chlorpyriphos and was the first attempt to identify the mechanism of resistance (target site insensitivity) in cattle tick populations from Minas Gerais state (Southeastern Brazil). Engorged female ticks were collected from 10 ranches within the state of Minas Gerais, and susceptibility was evaluated with the larval packet test (LPT) using technical grade cypermethrin and chlorpyriphos. It was possible to analyze LPT results of seven populations. Target site insensitivity was investigated in all 10 isolates by using molecular approaches for detection of the T2134A substitution within the domain III S6 segment and the C190A in the domain II S4-5 linker from the para-type sodium channel gene. LPT showed that all seven populations were resistant to cypermethrin with resistance ratio (RR) ranging from 16.0 to 25.0 and 85.7% were resistant to chlorpyriphos (RR = 2.2?15.6). Although the T2134A mutation was not detected, the C190A mutation was highly prevalent, being present in 82?100% of the alleles sampled in field populations. A significant correlation was found between the LC50 values for cypermethrin and the frequency of the C190A mutation suggesting that it might be responsible for the phenotypic resistance detected.
650 $acypermethrin
653 $aAllele-specific PCR
653 $aCattle tick
653 $aChlorpyriphos
653 $aLarval packet test
653 $aTarget site insensitivity
700 1 $aBRASIL, B. dos S. A. F.
700 1 $aBELLO, A. C. P. de P.
700 1 $aCUNHA, A. P. da
700 1 $aBARROS, A. T. M. de
700 1 $aLEITE, R. C.
700 1 $aSILAGHI, C.
700 1 $aPFISTER, K.
700 1 $aPASSOS, L. M. F.
773 $tVeterinary Parasitology$gv. 189, n.2-4, p. 327-332, 2012.
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Registro original: |
Embrapa Pantanal (CPAP) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
15/10/2018 |
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Data da última atualização: |
15/10/2018 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
SILVA, T. A.; MAIA, G. G.; CAMARGO, L. S. de A.; SIQUEIRA, L. G. B. |
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Afiliação: |
T. A. Silva; G. G. Maia; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; LUIZ GUSTAVO BRUNO SIQUEIRA, CNPGL. |
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Título: |
Associação entre o risco de estresse calórico, estação do ano e taxa de concepção em vacas leiteiras submetidas a Inseminação Artificial em Tempo Fixo. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
In: XXII Workshop de Iniciação Científica da Embrapa Gado de Leite. XXII. Anais. Juiz de Fora/MG. Embrapa Gado de Leite, 2018. 4 p |
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Idioma: |
Português |
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Palavras-Chave: |
Concepção; Estresse calórico; Vacas leiteiras. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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Marc: |
LEADER 00694naa a2200181 a 4500
001 2097392
005 2018-10-15
008 2018 bl uuuu u00u1 u #d
100 1 $aSILVA, T. A.
245 $aAssociação entre o risco de estresse calórico, estação do ano e taxa de concepção em vacas leiteiras submetidas a Inseminação Artificial em Tempo Fixo.
260 $c2018
653 $aConcepção
653 $aEstresse calórico
653 $aVacas leiteiras
700 1 $aMAIA, G. G.
700 1 $aCAMARGO, L. S. de A.
700 1 $aSIQUEIRA, L. G. B.
773 $tIn: XXII Workshop de Iniciação Científica da Embrapa Gado de Leite. XXII. Anais. Juiz de Fora/MG. Embrapa Gado de Leite, 2018. 4 p
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