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Registros recuperados : 30 | |
5. | | MACHADO, G. M.; CAIXETA, E. S.; FRANCO, M. M.; RUMPF, R.; DODE, M. A. N. Cultivo embrionário In vitro pós-eclosão até D15: desenvolvimento e sexo de embriões bovinos. Acta Scientiae Veterinariae, v. 36, supl. 2, p. S508, 2008. Edição dos resumos da 22. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Guarujá, SP, 2008. Apresentado também no: In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 13., 2008, Brasília,... Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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6. | | MACHADO, G. M.; CAIXETA, E. S.; FRANCO, M. M.; RUMPF, R.; DODE, M. A. N. Efeito do sexo na expressão de genes em embriões bovinos produzidos in vitro pós-eclosão. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 14., 2009, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2009. Resumo 040. p. 70 Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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9. | | CAIXETA, E. S.; FIGUEIREDO, P. R.; FRANCO, M. M.; BURATINI JÚNIOR, J.; DODE, M. A. N. Expression of candidate genes for bovine oocyte competence during follicular development. Animal Reproduction, v. 6, n. 1, p. 276, Jan./Mar. 2009. Edição dos Abstracts do II International Symposium on animal Biology or Reproduction, São Paulo, nov. 2008. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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10. | | CAIXETA, E. S.; SIQUEIRA FILHO, E.; FRANCO, M. M.; DODE, M. A. N.; RUMPF, R. Influência do momento da primeira clivagem no desenvolvimento e sua relação com o sexo dos embriões em bovinos. Acta Scientiae Veterinariae, v. 36, supl. 2, p. S496, 2008. Edição dos resumos da 22. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Guarujá, SP, 2008. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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12. | | CAIXETA, E. S.; LEME, L. O.; MACHADO, G. M.; RUMPF, T.; FRANCO, M. M.; DODE, M. A. N. Competência ovocitária de acordo com o tamanho folicular: resultados parciais. Acta Scientiae Veterinariae, Porto Alegre, v. 35, supl. 3, p. S1156, 2007. Edição dos resumos do XXI Annual Meeting of the Brazilian Embryo Technology Society (SBTE), Salvador, BA, ago. 2007. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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13. | | CAIXETA, E. S.; FIGUEIREDO, P. R.; MACHADO, G. M.; FRANCO, M. M.; BURATINI, J.; DODE, A. N. Expression of candidate genes for oocyte competence in bovine Cumulus cells. Reproduction, Fertility and Development, v.21, n.1, p. 189, 2009. Edição dos proceedings da Annual Conference or the International Embryo Transfer Society, San Diego, California, 2009. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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15. | | SIQUEIRA FILHO, E.; CAIXETA, E. S.; PRIBENSZKY, C.; MOLNAR, M.; HORVATH, A.; HARNOS, A.; FRANCO, M. M.; RUMPF, R. Vitrification of bovine blastocysts pretreated with sublethal hydrostatic pressure stress: evaluation of post-thaw in vitro development and gene expression. Reproduction, Fertility and Development, v. 23, p. 585-590, 2011. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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16. | | CARVALHO, J. O.; SIQUEIRA FILHO, E.; MACHADO, G. M.; CAIXETA, E. S.; RUMPF, R.; McMANUS, C. M.; SARTORI, R.; DODE, M. A. N. Avaliação de parâmetros da qualidade espermática para predizer a fertilidade in vitro do sêmen bovino congelado. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 13., 2008, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2008. Resumo 055. p. 97. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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17. | | SUDANO, M. J.; CAIXETA, E. S.; PASCHOAL, D. M.; MARTINS JÚNIOR, A.; MACHADO, R.; BURATINI, J.; LANDIM-ALVARENGA, F. D. C. Cryotolerance and global gene-expression patterns of Bos taurus indicus and Bos taurus taurus in vitroand in vivo-produced blastocysts. Reproduction, Fertility and Development, v. 26, p. 1129-1141,2013. Biblioteca(s): Embrapa Pecuária Sudeste. |
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18. | | CARVALHO, J. O.; MACHADO, G. M.; SIQUEIRA FILHO, E.; CAIXETA, E. S.; FRANCO, M. M.; RUMPF, R.; DODE, M. A. N. Gradiente de Percoll em diferentes volumes, tempo e força de centrifugação na produção In vitro e no sexo de embriões bovinos. Acta Scientiae Veterinariae, v. 36, supl. 2, p. S514, 2008 Edição dos resumos da 22. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Guarujá, SP, 2008. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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19. | | FRANCO, M. M.; FAGUNDES, N. S.; MICHALCZECHEN-LACERDA, V. A.; CAIXETA, E. S.; RODRIGUES, F. de C.; MACHADO, G. M.; FERREIRA, A. R.; DODE, M. A. N. Characterisation of the methylation pattern in the intragenic CpG island of the IGF2 gene in Bos taurus indicus cumulus cells during in vitro maturation. Journal of Assisted Reproduction and Genetics, v. 31, n. 1, p. 115-120, 2014. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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20. | | FAGUNDES, N. S.; MICHALCZECHEN-LACERDA, V. A.; CAIXETA, E. S.; MACHADO, G. M.; RODRIGUES, F. C.; MELO, E. de O.; DODE, M. A. N.; FRANCO, M. M. Methylation status in the intragenic differentially methylated region of the IGF2 locus in Bos taurus indicus oocytes with different developmental competencies. Molecular Human Reproduction, v. 17, n. 2, p. 85-91, 2011. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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Registros recuperados : 30 | |
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Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
22/02/2013 |
Data da última atualização: |
07/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
MACHADO, G. M.; CAIXETA, E. S.; LUCCI, C. M.; RUMPF, R.; FRANCO, M. M.; DODE, M. A. N. |
Afiliação: |
GRAZIELI MARINHEIRO MACHADO, UnB; ESTER SIQUEIRA CAIXETA, UnB; CAROLINA MADEIRA LUCCI, UnB; RODOLFO RUMPF, CENARGEN; MAURICIO MACHAIM FRANCO, CENARGEN; MARGOT ALVES NUNES DODE, CENARGEN. |
Título: |
Post-hatching development of bovine embryos in vitro: the effects of tunnel preparation and gender. |
Ano de publicação: |
2011 |
Fonte/Imprenta: |
Zygote, v. 20, p. 123-134, 2011. |
Idioma: |
Inglês |
Conteúdo: |
The objective of this study was to compare morphological characteristics, kinetics of development, and gene expression ofmale and female IVP embryos thatwere cultured until day (D)15 (fertilization = D0), using either phosphate-buffered saline (PBS) or Milli-Q water (MQW) to dilute the agarose gel used for tunnel construction. On D11, embryos (n = 286) were placed in agarose gel tunnels diluted in PBS and MQW. Embryos were evaluated for morphology, and embryo size was recorded on D11, D12.5, D14 and D15. Then, embryos were sexed and used for gene expression analyses (G6PD, GLUT1, GLUT3, PGK1, PLAC8, KRT8, HSF1 and IFNT). The percentage of elongated embryos at D15 was higher (p < 0.05) in the PBS (54%) than in theMQW(42%) gel. However, embryos produced inMQWwere bigger (p < 0.05) and had a lower expression of GLUT1 (p = 0.08) than those cultured in PBS. There was a higher proportion of male than female embryos at D15 in both treatments, MQW(65% vs. 35%; p < 0.05) and PBS (67% vs. 33%; p < 0.05); however, embryo size was not signi?cantly different between genders. Moreover, D15 female embryos had greater expression of G6PD (p = 0.05) and KRT8 (p = 0.03) than male embryos. In conclusion, the diluent used for tunnel construction affected embryo development in the post-hatching development (PHD) system, and the use ofMQWwas the most indicative measure for the evaluation of embryo quality. Male and female embryos cultured from D11 to D15, either in an MQW or PBS agarose gel, demonstrated similar development but different gene expression. MenosThe objective of this study was to compare morphological characteristics, kinetics of development, and gene expression ofmale and female IVP embryos thatwere cultured until day (D)15 (fertilization = D0), using either phosphate-buffered saline (PBS) or Milli-Q water (MQW) to dilute the agarose gel used for tunnel construction. On D11, embryos (n = 286) were placed in agarose gel tunnels diluted in PBS and MQW. Embryos were evaluated for morphology, and embryo size was recorded on D11, D12.5, D14 and D15. Then, embryos were sexed and used for gene expression analyses (G6PD, GLUT1, GLUT3, PGK1, PLAC8, KRT8, HSF1 and IFNT). The percentage of elongated embryos at D15 was higher (p < 0.05) in the PBS (54%) than in theMQW(42%) gel. However, embryos produced inMQWwere bigger (p < 0.05) and had a lower expression of GLUT1 (p = 0.08) than those cultured in PBS. There was a higher proportion of male than female embryos at D15 in both treatments, MQW(65% vs. 35%; p < 0.05) and PBS (67% vs. 33%; p < 0.05); however, embryo size was not signi?cantly different between genders. Moreover, D15 female embryos had greater expression of G6PD (p = 0.05) and KRT8 (p = 0.03) than male embryos. In conclusion, the diluent used for tunnel construction affected embryo development in the post-hatching development (PHD) system, and the use ofMQWwas the most indicative measure for the evaluation of embryo quality. Male and female embryos cultured from D11 to D15, either in an MQW or PBS agarose gel, demon... Mostrar Tudo |
Palavras-Chave: |
Cultura do embrião; Sexagem. |
Thesagro: |
Gado. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/179338/1/posthatching-development-of-bovine-embryos-in-vitro-the-effects-of-tunnel-preparation-and-gender.pdf
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Marc: |
LEADER 02172naa a2200217 a 4500 001 1950734 005 2023-03-07 008 2011 bl uuuu u00u1 u #d 100 1 $aMACHADO, G. M. 245 $aPost-hatching development of bovine embryos in vitro$bthe effects of tunnel preparation and gender.$h[electronic resource] 260 $c2011 520 $aThe objective of this study was to compare morphological characteristics, kinetics of development, and gene expression ofmale and female IVP embryos thatwere cultured until day (D)15 (fertilization = D0), using either phosphate-buffered saline (PBS) or Milli-Q water (MQW) to dilute the agarose gel used for tunnel construction. On D11, embryos (n = 286) were placed in agarose gel tunnels diluted in PBS and MQW. Embryos were evaluated for morphology, and embryo size was recorded on D11, D12.5, D14 and D15. Then, embryos were sexed and used for gene expression analyses (G6PD, GLUT1, GLUT3, PGK1, PLAC8, KRT8, HSF1 and IFNT). The percentage of elongated embryos at D15 was higher (p < 0.05) in the PBS (54%) than in theMQW(42%) gel. However, embryos produced inMQWwere bigger (p < 0.05) and had a lower expression of GLUT1 (p = 0.08) than those cultured in PBS. There was a higher proportion of male than female embryos at D15 in both treatments, MQW(65% vs. 35%; p < 0.05) and PBS (67% vs. 33%; p < 0.05); however, embryo size was not signi?cantly different between genders. Moreover, D15 female embryos had greater expression of G6PD (p = 0.05) and KRT8 (p = 0.03) than male embryos. In conclusion, the diluent used for tunnel construction affected embryo development in the post-hatching development (PHD) system, and the use ofMQWwas the most indicative measure for the evaluation of embryo quality. Male and female embryos cultured from D11 to D15, either in an MQW or PBS agarose gel, demonstrated similar development but different gene expression. 650 $aGado 653 $aCultura do embrião 653 $aSexagem 700 1 $aCAIXETA, E. S. 700 1 $aLUCCI, C. M. 700 1 $aRUMPF, R. 700 1 $aFRANCO, M. M. 700 1 $aDODE, M. A. N. 773 $tZygote$gv. 20, p. 123-134, 2011.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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