| |
|
|
| Registros recuperados : 5 | |
| 1. |  | BATISTA, A. R. F.; BRITO, E. S. de; RICARDO, N. M. P. S.; CABRAL, E. C.; EBERLIN, M. N.; PILAU, E. J.; GOZZO, F. C. Análise do perfil químico e físico da cera de carnaúba (Copernicia sp.) obtida por diferentes acessos. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA AGROINDÚSTRIA TROPICAL, 8., 2010, Fortaleza. Resumos. Fortaleza: Embrapa Agroindústria Tropical, 2010.| Biblioteca(s): Embrapa Agroindústria Tropical. |
|    |
| 2. | | SCHIOZER, A. L.; CABRAL, E. C.; GODOY, L. A. F. de; CHAVES, F. C. M.; POPPI, R. J.; RIVEROS, J. M.; EBERLIN, M. N.; BARATA, L. E. S. Electrospray ionization mass spectrometry fingerprinting of extracts of the leaves of Arrabidaea chica. Journal of the Brazilian Chemical Society, v. 23, n. 3, p. 409-414 + S1-S11, 2012.| Biblioteca(s): Embrapa Amazônia Ocidental. |
| |
| 3. | | LEÃO, B. C. S.; FRIGONI, N. A. S. R.; NOGUEIRA, E.; CABRAL, E. C.; FERREIRA, C. R.; EBERLIN, M. N.; ACCORSI, M. F.; NEVES, T. V.; MINGOTI, G. Z. Membrane lipid profile of in vitro-produced embryos is affected by vitrification but not by long-term dietary supplementation of polyunsaturated fatty acids for oocyte donor beef heifers. Reproduction, Fertility and Development, v. 29, n.6, p. 1217-1230, 2017.| Biblioteca(s): Embrapa Pantanal. |
|  |
| 4. | | LEÃO, B. C. S.; ROCHA-FRIGONI, N. A. S.; NOGUEIRA, E.; CABRAL, E. C.; FERREIRA, C. R.; EBERLIN, M. N.; ACCORSI, M. F.; NEVES, T. V.; MINGOTI, G. Z. Effect of dietary supplementation of Nelore heifers with polyunsaturated fatty acids on cryotolerance and membrane lipid profile of fresh and vitrified in vitro-produced embryos. Animal Reproduction, v.13, n.3, p.700, Jul./Sept. 2016. Proceedings of the 30th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Foz do Iguaçu, PR, Brazil, August 25th to 27th, 2016, and 32nd Meeting of the European Embryo Transfer Association (AETE); Barcelona, Spain,...| Biblioteca(s): Embrapa Pantanal. |
| |
| 5. | | LEÃO, B. C. S.; ROCHA-FRIGONI, N. A. S.; CABRAL, E. C.; COELHO, M. B.; FERREIRA, C. R.; EBERLIN, M. N.; ACCORSI, M. F.; NOGUEIRA, E.; MINGOTI, G. Z. Improved embryonic cryosurvival observed after in vitro supplementation with conjugated linoleic acid is related to changes in the membrane lipid profile. Theriogenology, v. 84, n. 1, p. 127-136, 2015.| Biblioteca(s): Embrapa Pantanal. |
| |
| Registros recuperados : 5 | |
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Pantanal. Para informações adicionais entre em contato com cpap.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Pantanal. |
|
Data corrente: |
27/11/2015 |
|
Data da última atualização: |
17/03/2016 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 1 |
|
Autoria: |
LEÃO, B. C. S.; ROCHA-FRIGONI, N. A. S.; CABRAL, E. C.; COELHO, M. B.; FERREIRA, C. R.; EBERLIN, M. N.; ACCORSI, M. F.; NOGUEIRA, E.; MINGOTI, G. Z. |
|
Afiliação: |
UNESP; UNESP; UNICAMP; UNICAMP; UNICAMP; UNICAMP; UNESP; ERIKLIS NOGUEIRA, CPAP; UNESP. |
|
Título: |
Improved embryonic cryosurvival observed after in vitro supplementation with conjugated linoleic acid is related to changes in the membrane lipid profile. |
|
Ano de publicação: |
2015 |
|
Fonte/Imprenta: |
Theriogenology, v. 84, n. 1, p. 127-136, 2015. |
|
DOI: |
10.1016/j.theriogenology.2015.02.023 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The aim of this study was to evaluate the effect of supplementing serum-containing media with a mixture of cis- and trans-9,11- and -10,12?conjugated isomers of linoleic acid (CLA) during different steps of the in vitro production (IVM, IVC, or IVM þ IVC) of bovine embryos on their embryonic development, cryotolerance, and lipid profile. To evaluate the impact of the CLA on membrane lipids, such as phosphatidylcholine (PC) and sphingomyelin (SM), the embryos? lipid profiles were obtained using matrix-assisted laser desorption ionization mass spectrometry. The cleavage rates (78.6%?84.8%) and blastocyst development (44.8%?51.2%) remained unaltered. The postthawing reexpansion rates were higher (P < 0.05) when the CLA was added to the IVM medium (82.6%) or to the IVM þ IVC medium (83.8%) than the control (69.3%) or IVC medium (63.0%). Changes in the blastocysts? lipid profile occurred when supplementation was restricted to the IVM or IVC medium. However, the most prominent effects of the CLA on the embryonic PC and SM profiles were observed when the supplement was added to IVM þ IVC media, which was an increase in the level of highly unsaturated PCs containing 36 or 38 carbons, which are likely to contain CLA residues. These results showed that the molecular mechanism resulting in the improved cryosurvival, observed with CLA supplementation during bovine embryonic in vitro production, was related to the composition of structural lipids of cellular membranes and is
dependent on the treatment length. Monitoring the lipid profile of embryonic membranes may improve the CLA supplementation strategy and facilitate the development of new IVC systems to improve the cryopreservation of bovine embryos and those of other domestic species. MenosThe aim of this study was to evaluate the effect of supplementing serum-containing media with a mixture of cis- and trans-9,11- and -10,12?conjugated isomers of linoleic acid (CLA) during different steps of the in vitro production (IVM, IVC, or IVM þ IVC) of bovine embryos on their embryonic development, cryotolerance, and lipid profile. To evaluate the impact of the CLA on membrane lipids, such as phosphatidylcholine (PC) and sphingomyelin (SM), the embryos? lipid profiles were obtained using matrix-assisted laser desorption ionization mass spectrometry. The cleavage rates (78.6%?84.8%) and blastocyst development (44.8%?51.2%) remained unaltered. The postthawing reexpansion rates were higher (P < 0.05) when the CLA was added to the IVM medium (82.6%) or to the IVM þ IVC medium (83.8%) than the control (69.3%) or IVC medium (63.0%). Changes in the blastocysts? lipid profile occurred when supplementation was restricted to the IVM or IVC medium. However, the most prominent effects of the CLA on the embryonic PC and SM profiles were observed when the supplement was added to IVM þ IVC media, which was an increase in the level of highly unsaturated PCs containing 36 or 38 carbons, which are likely to contain CLA residues. These results showed that the molecular mechanism resulting in the improved cryosurvival, observed with CLA supplementation during bovine embryonic in vitro production, was related to the composition of structural lipids of cellular membranes and is
dependent on... Mostrar Tudo |
|
Palavras-Chave: |
Animal embryos; Bovine embryo; Cryosurvival; In vitro production; Lipid profile. |
|
Thesagro: |
Bovino; Embrião animal; Reprodução animal. |
|
Thesaurus NAL: |
Cattle; conjugated linoleic acid; In vitro fertilization; mass spectrometry. |
|
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 02882naa a2200373 a 4500
001 2029790
005 2016-03-17
008 2015 bl --- 0-- u #d
024 7 $a10.1016/j.theriogenology.2015.02.023$2DOI
100 1 $aLEÃO, B. C. S.
245 $aImproved embryonic cryosurvival observed after in vitro supplementation with conjugated linoleic acid is related to changes in the membrane lipid profile.
260 $c2015
520 $aThe aim of this study was to evaluate the effect of supplementing serum-containing media with a mixture of cis- and trans-9,11- and -10,12?conjugated isomers of linoleic acid (CLA) during different steps of the in vitro production (IVM, IVC, or IVM þ IVC) of bovine embryos on their embryonic development, cryotolerance, and lipid profile. To evaluate the impact of the CLA on membrane lipids, such as phosphatidylcholine (PC) and sphingomyelin (SM), the embryos? lipid profiles were obtained using matrix-assisted laser desorption ionization mass spectrometry. The cleavage rates (78.6%?84.8%) and blastocyst development (44.8%?51.2%) remained unaltered. The postthawing reexpansion rates were higher (P < 0.05) when the CLA was added to the IVM medium (82.6%) or to the IVM þ IVC medium (83.8%) than the control (69.3%) or IVC medium (63.0%). Changes in the blastocysts? lipid profile occurred when supplementation was restricted to the IVM or IVC medium. However, the most prominent effects of the CLA on the embryonic PC and SM profiles were observed when the supplement was added to IVM þ IVC media, which was an increase in the level of highly unsaturated PCs containing 36 or 38 carbons, which are likely to contain CLA residues. These results showed that the molecular mechanism resulting in the improved cryosurvival, observed with CLA supplementation during bovine embryonic in vitro production, was related to the composition of structural lipids of cellular membranes and is dependent on the treatment length. Monitoring the lipid profile of embryonic membranes may improve the CLA supplementation strategy and facilitate the development of new IVC systems to improve the cryopreservation of bovine embryos and those of other domestic species.
650 $aCattle
650 $aconjugated linoleic acid
650 $aIn vitro fertilization
650 $amass spectrometry
650 $aBovino
650 $aEmbrião animal
650 $aReprodução animal
653 $aAnimal embryos
653 $aBovine embryo
653 $aCryosurvival
653 $aIn vitro production
653 $aLipid profile
700 1 $aROCHA-FRIGONI, N. A. S.
700 1 $aCABRAL, E. C.
700 1 $aCOELHO, M. B.
700 1 $aFERREIRA, C. R.
700 1 $aEBERLIN, M. N.
700 1 $aACCORSI, M. F.
700 1 $aNOGUEIRA, E.
700 1 $aMINGOTI, G. Z.
773 $tTheriogenology$gv. 84, n. 1, p. 127-136, 2015.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Pantanal (CPAP) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
