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Registros recuperados : 88 | |
61. | | BEVITORI, R.; SIRCAR, S.; TOGAWA, R. C.; CÔRTES, M. V. de C. B.; OLIVEIRA, T. S.; GROSSI-DE-SÁ, M. F.; PAREKH, N. Identification of co-expression gene networks controlling rice blast disease during an incompatible reaction. Genetics and Molecular Research, v. 19, n. 3, gmr18579, 2020. Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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62. | | FREITAS-SILVA, O.L.; ROCHA, E. S. da; CÔRTES, M. V. de C. B.; FREIRE JR. M.; BITTENCOURT, A. M.; PIRES, L. F. G.; VITAL, H. C. Radiação gama no controle in vitro e in vivo de Colletotrichum gloeosporioides Penz. e Botryodiplodia theobromae em frutos de manga cv. "Tommy Atkins". In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 17., 2002, Belém, PA. Os novos desafios da fruticultura brasileira: anais. Belém: SBF, 2002. Cópia de trabalho editado em CD-ROM. Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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63. | | ARRIEL-ELIAS, M. T.; OLIVEIRA, M. I. S.; SILVA-LOBO, V. L.; FILIPPI, M. C. C.; BABANA, A. H.; CONCEIÇÃO, E. C.; CORTES, M. V. de C. B. Shelf life enhancement of plant growth promoting rhizobacteria using a simple formulation screening method. African Journal of Microbiology Research, v. 12, n. 5, p. 115-126, Feb. 2018. Biblioteca(s): Embrapa Arroz e Feijão. |
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64. | | ARRUDA, R. L.; RODRIGUES, M. C. de M.; CORTES, M. V. de C. B.; FILIPPI, M. C. C. de; MASCARIN, G. M.; CONCEIÇÃO, E. C. da. Plant extract of Ruta graveolens standardized in furanocoumarins: resistance elicitorin rice plants against Magnaporthe oryzae. International Journal of Research Studies in Biosciences, v. 5, n. 8, p. 11-21, Aug. 2017. Biblioteca(s): Embrapa Arroz e Feijão. |
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66. | | CHAIBUB, A. A.; SENA, A. P. A.; CORTES, M. V. de C. B.; FILIPPI, M. C. C. de; ARAÚJO, L. G. de. Supressão de brusone foliar em arroz com o uso de Epicoccum sp. In: SEMINÁRIO JOVENS TALENTOS, 7., 2013, Santo Antônio de Goiás. Coletânea dos resumos apresentados. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2013. p. 32. (Embrapa Arroz e Feijão. Documentos, 292). Apresentação oral - Pós-graduação. Biblioteca(s): Embrapa Arroz e Feijão. |
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67. | | MAGALHÃES, M. S.; NOGUEIRA, T. A.; FILIPPI, M. C. C. de; SILVA-LOBO, V. L. da; CORTES, M. V. de C. B.; PRABHU, A. S. Supressão das doenças de arroz por isolados de bactérias. In: SEMINÁRIO JOVENS TALENTOS, 6., 2012, Santo Antônio de Goiás. Resumos apresentados. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2012. p. 56-57. (Embrapa Arroz e Feijão. Documentos, 275). Biblioteca(s): Embrapa Arroz e Feijão. |
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70. | | ARRUDA, R. L.; SOUSA, T. P. de; FILIPPI, M. C. C. de; CORTES, M. V. de C. B.; CONCEIÇÃO, E. C. da. Utilização de extrato vegetal de Ruta graveolens L. no controle de Magnaporthe oryzae. In: SEMINÁRIO JOVENS TALENTOS, 10., 2016, Santo Antônio de Goiás. Coletânea dos resumos apresentados. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2016. p. 74. (Embrapa Arroz e Feijão. Documentos, 311). Biblioteca(s): Embrapa Arroz e Feijão. |
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71. | | CÔRTES, M. V. de C. B.; DUARTE, L. T.; FILIPPI, M. C. C. de; LOBO, V. L. da S.; PRABHU, A. S.; GONÇALVES, F. J.; FREITAS, B. R. de; GUIMARÃES, L. A. F.; WENDLAND, A. A coleção de isolados de Magnaporthe oryzae da Embrapa Arroz e Feijão: uma micoteca para uso na pesquisa com brusone. Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2010. 20 p. (Embrapa Arroz e Feijão. Documentos, 259). Biblioteca(s): Embrapa Arroz e Feijão. |
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72. | | SILVA LOBO, V. L.; PRABHU, A. S.; MORAIS, O. P. de; CASTRO, A. P. de; TORGA, P. P.; CORTES, M. V. de C. B.; CINTRA, M. M. D. F. Espectro de resistência à brusone de linhagens avançadas e cultivares de arroz irrigado e de terras altas. In: CONGRESSO BRASILEIRO DE ARROZ IRRIGADO, 8., 2013, Santa Maria. Avaliando cenários para a produção sustentável de arroz: anais. Santa Maria: UFSM; Porto Alegre: Sosbai, 2013. v.1. p. 558-562. Biblioteca(s): Embrapa Arroz e Feijão. |
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73. | | SOUSA, K. C. I.; ARAÚJO, L. G. de; CHAIBUB, A. A.; CARVALHO, J. C. B. de; SILVA, C. S.; CORTES, M. V. de C. B.; FILIPPI, M. C. C. de. Alterações enzimáticas relacionadas à resposta de defesa de plantas de arroz e supressão da brusone por fungos de controle biológico. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 8., 2015, Goiânia. O melhoramento de plantas, o futuro da agricultura e a soberania nacional: anais. Goiânia: UFG: SBMP, 2015. Biblioteca(s): Embrapa Arroz e Feijão. |
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74. | | SILVA, L. L. da; WENDLAND, A.; MELO, L. C.; COSTA, J. G. C. da; DEL PELOSO, M. J.; PEREIRA, H. S.; FARIA, L. C. de; CORTES, M. V. de C. B. Interação diferencial entre isolados de Xanthomonas axonopodis pv. phaseoli e genótipos de feijoeiro. In: CONGRESSO NACIONAL DE PESQUISA DE FEIJÃO, 9., 2008, Campinas. Ciência e tecnologia na cadeia produtiva do feijão. Campinas: Instituto Agronômico, 2008. (IAC. Documentos, 85). Biblioteca(s): Embrapa Arroz e Feijão. |
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75. | | VANEGAS K. G.; RENDSVIG, J. K. H.; JARCZYSKA, Z. D.; CÔRTES, M. V. de C. B.; VAN ESCH, A. P.; MORERA-GÓMEZ, M.; CONTESINI, F. J.; MORTENSEN, U. H. A Mad7 system for genetic engineering of filamentous fungi. Journal of Fungi, v. 9, n. 1, 16, 2023. Biblioteca(s): Embrapa Arroz e Feijão. |
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76. | | OLIVEIRA, M. I. de S.; CHAIBUB, A. A.; SOUSA, T. P.; CORTES, M. V. de C. B.; SOUZA, A. C. A. de; CONCEIÇÃO, E. C. da; FILIPPI, M. C. C. de. Formulações contendo Pseudomonas fluorescens e Burkholderia pyrrocinia para o manejo integrado da brusone do arroz de terras altas. In: SEMINÁRIO JOVENS TALENTOS, 13., 2019, Santo Antônio de Goiás. Resumos... Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2019. p. 96. Biblioteca(s): Embrapa Arroz e Feijão. |
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77. | | OLIVEIRA, M. I. de S.; CHAIBUB, A. A.; SOUSA, T. P.; CÔRTES, M. V. de C. B.; SOUZA, A. C. A. de; CONCEIÇÃO, E. C. da; FILIPPI, M. C. C. de. Formulations of Pseudomonas fluorescens and Burkholderia pyrrocinia control rice blast of upland rice cultivated under no-tillage system. Biological Control, v. 144, 104153, 2020. Biblioteca(s): Embrapa Arroz e Feijão. |
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78. | | MARQUES, M. L. da S.; JESUS, J. M. I. de; OLIVEIRA, M. I. de S.; CÔRTES, M. V. de C. B.; FILIPPI, M. C. C. de; ROCHA, M. R. da. Biochemical response of resistant and susceptible Capsicum spp. to Meloidogyne enterolobii. Journal of Phytopathology, v. 171, n. 9, p. 430-441, Sept. 2023. Biblioteca(s): Embrapa Arroz e Feijão. |
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79. | | ALENCAR, N. E.; WENDLAND, A.; MELO, L. C.; COSTA, J. G. C.; DEL PELOSO, M. J.; PEREIRA, H. S.; FARIA, L. C. de; CORTES, M. V. de C. B. Avaliação fenotípica de genótipos de feijoeiro comum a isolados de Curtobacterium flaccumfaciens pv. flaccumfaciens. In: CONGRESSO NACIONAL DE PESQUISA DE FEIJÃO, 9., 2008, Campinas. Ciência e tecnologia na cadeia produtiva do feijão. Campinas: Instituto Agronômico, 2008. 1 CD-ROM. (IAC. Documentos, 85). Biblioteca(s): Embrapa Arroz e Feijão. |
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Registros recuperados : 88 | |
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Registro Completo
Biblioteca(s): |
Embrapa Arroz e Feijão. |
Data corrente: |
06/11/2020 |
Data da última atualização: |
30/04/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
CÔRTES, M. V. de C. B.; OLIVEIRA, M. I. de S.; MATEUS, J. R.; SELDIN, L.; SILVA-LOBO, V. L.; FREIRE, D. M. G. |
Afiliação: |
MARCIO VINICIUS DE C BARROS CORTES, CNPAF; MAYTHSULENE INACIO DE SOUSA OLIVEIRA, bolsista CNPAF; JACKELINE ROSSETTI MATEUS, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO; LUCY SELDIN, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO; VALACIA LEMES DA SILVA LOBO, CNPAF; DENISE MARIA GUIMARAES FREIRE, UNIVERSIDADE FEDERAL DO RIO DE JANEIRO. |
Título: |
A pipeline for the genetic improvement of a biological control agent enhances its potential for controlling soil-borne plant pathogens. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Biological Control, v. 152, 104460, 2021. |
ISSN: |
1049-9644 |
DOI: |
https://doi.org/10.1016/j.biocontrol.2020.104460 |
Idioma: |
Inglês |
Conteúdo: |
Use of biopesticides results in a reduction of the worldwide dependence on chemical pesticides, ending in a more sustainable crop protection. For this reason, biological control industry invests in continued research for more effective biocontrol agents (BCAs) using basically two strategies: isolation of new strains from nature or conventional genetic improvement. However, both are highly onerous and time-consuming. Here we show a pipeline for the genetic improvement of fungal BCAs, based on genome shuffling, the most recent and promising non-recombinant DNA technology for the rapid phenotype improvement of microbial strains. The method consisted of the construction of a parent library using mutagenic agents, followed by genome shuffling and high-throughput screening. Sarocladium oryzae BRM 6461, a known cerulenin antifungal producer strain and high biocontrol potential, served as the model fungal BCA. The pipeline aimed to select mutant enhanced strains at least one of these three desirable characteristics: antagonism, UV-B irradiation tolerance and high temperature tolerance. The experiments were conducted in laboratory and green house. After four cycles of genome shuffling we selected the superior S. oryzae GS4-03 strain, showing cerulenin production of 203.3 ± 1.4 µg/mL, 42% higher than the wild-type strain BRM 6461. The GS4-03 strain exhibited good genetic stability for at least five successive subcultivation assays. Antagonism assay showed an increased micelial inhibition of Rhizoctonia solani and Sclerotinia sclerotiorum when using GS4-03 strain compared to the BRM 6461 strain. The bioassays, showed that the superior strain GS4-03 have an increased ability to control root rot (23.4%) and white mold (8.1 times) disease compared to the wild type strain BRM 6461. Assessment of virulence of the superior strain GS4-03 against rice plants showed that improvement procedures did not change the original strain behavior in this subject. Hydrolytic activity of enzymes related to the biocontrol action as chitinase, protease and B-1,3-glucanase did not present statistical difference between GS4-03 and BRM 6461 strains. Results of dual assay culture between GS4-03 and BRM 6461 strains showed that both have different genetic backgrounds, although RAPD and ITS-rDNA tests were not fully efficient to distinguish genetically the strains. Moreover, mutations resulted in S. oryzae strains more tolerant to UV-B irradiation, but with low genetic stability. Our results report for the first time an efficient pipeline for the genetic improvement of fungal BCAs based on genome shuffling. MenosUse of biopesticides results in a reduction of the worldwide dependence on chemical pesticides, ending in a more sustainable crop protection. For this reason, biological control industry invests in continued research for more effective biocontrol agents (BCAs) using basically two strategies: isolation of new strains from nature or conventional genetic improvement. However, both are highly onerous and time-consuming. Here we show a pipeline for the genetic improvement of fungal BCAs, based on genome shuffling, the most recent and promising non-recombinant DNA technology for the rapid phenotype improvement of microbial strains. The method consisted of the construction of a parent library using mutagenic agents, followed by genome shuffling and high-throughput screening. Sarocladium oryzae BRM 6461, a known cerulenin antifungal producer strain and high biocontrol potential, served as the model fungal BCA. The pipeline aimed to select mutant enhanced strains at least one of these three desirable characteristics: antagonism, UV-B irradiation tolerance and high temperature tolerance. The experiments were conducted in laboratory and green house. After four cycles of genome shuffling we selected the superior S. oryzae GS4-03 strain, showing cerulenin production of 203.3 ± 1.4 µg/mL, 42% higher than the wild-type strain BRM 6461. The GS4-03 strain exhibited good genetic stability for at least five successive subcultivation assays. Antagonism assay showed an increased micelial inhibit... Mostrar Tudo |
Thesagro: |
Controle Biológico; Mofo Branco; Patógeno. |
Thesaurus NAL: |
Biological control; Genetics; Genome; Root rot; Sarocladium oryzae. |
Categoria do assunto: |
H Saúde e Patologia |
Marc: |
LEADER 03540naa a2200301 a 4500 001 2126356 005 2021-04-30 008 2021 bl uuuu u00u1 u #d 022 $a1049-9644 024 7 $ahttps://doi.org/10.1016/j.biocontrol.2020.104460$2DOI 100 1 $aCÔRTES, M. V. de C. B. 245 $aA pipeline for the genetic improvement of a biological control agent enhances its potential for controlling soil-borne plant pathogens.$h[electronic resource] 260 $c2021 520 $aUse of biopesticides results in a reduction of the worldwide dependence on chemical pesticides, ending in a more sustainable crop protection. For this reason, biological control industry invests in continued research for more effective biocontrol agents (BCAs) using basically two strategies: isolation of new strains from nature or conventional genetic improvement. However, both are highly onerous and time-consuming. Here we show a pipeline for the genetic improvement of fungal BCAs, based on genome shuffling, the most recent and promising non-recombinant DNA technology for the rapid phenotype improvement of microbial strains. The method consisted of the construction of a parent library using mutagenic agents, followed by genome shuffling and high-throughput screening. Sarocladium oryzae BRM 6461, a known cerulenin antifungal producer strain and high biocontrol potential, served as the model fungal BCA. The pipeline aimed to select mutant enhanced strains at least one of these three desirable characteristics: antagonism, UV-B irradiation tolerance and high temperature tolerance. The experiments were conducted in laboratory and green house. After four cycles of genome shuffling we selected the superior S. oryzae GS4-03 strain, showing cerulenin production of 203.3 ± 1.4 µg/mL, 42% higher than the wild-type strain BRM 6461. The GS4-03 strain exhibited good genetic stability for at least five successive subcultivation assays. Antagonism assay showed an increased micelial inhibition of Rhizoctonia solani and Sclerotinia sclerotiorum when using GS4-03 strain compared to the BRM 6461 strain. The bioassays, showed that the superior strain GS4-03 have an increased ability to control root rot (23.4%) and white mold (8.1 times) disease compared to the wild type strain BRM 6461. Assessment of virulence of the superior strain GS4-03 against rice plants showed that improvement procedures did not change the original strain behavior in this subject. Hydrolytic activity of enzymes related to the biocontrol action as chitinase, protease and B-1,3-glucanase did not present statistical difference between GS4-03 and BRM 6461 strains. Results of dual assay culture between GS4-03 and BRM 6461 strains showed that both have different genetic backgrounds, although RAPD and ITS-rDNA tests were not fully efficient to distinguish genetically the strains. Moreover, mutations resulted in S. oryzae strains more tolerant to UV-B irradiation, but with low genetic stability. Our results report for the first time an efficient pipeline for the genetic improvement of fungal BCAs based on genome shuffling. 650 $aBiological control 650 $aGenetics 650 $aGenome 650 $aRoot rot 650 $aSarocladium oryzae 650 $aControle Biológico 650 $aMofo Branco 650 $aPatógeno 700 1 $aOLIVEIRA, M. I. de S. 700 1 $aMATEUS, J. R. 700 1 $aSELDIN, L. 700 1 $aSILVA-LOBO, V. L. 700 1 $aFREIRE, D. M. G. 773 $tBiological Control$gv. 152, 104460, 2021.
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