Data/hora: 15/06/2024 08:12:44

Biblioteca(s): 	Embrapa Pantanal.	
Data corrente: 	05/01/2004	
Data da última atualização: 	31/07/2013	
Autoria: 	LARA, M. A. C.; NARDON, R. F.; BARUFALDI, E.; CARBINATTO, M. D.; BUFARAH, G.; DEMARCHI, J. J. A. A.; ALLEONI, G. F.; SERENO, J. R.; SANTOS, S. A.; ABREU, U. G. P.; FIGUEIREDO, L. A.; RAZOOK, A. G.	
Afiliação: 	Instituto de Zootecnia (Nova Odessa, SP). U.M.P. (Piracicaba, SP). P.R.D.T.E.O/APTA/ SAA (Andradina, SP). Embrapa Pantanal (Corumba, MS). C.A.P.T.A. B.C./IZ/APTA/SAA (Sertaozinho, SP).	
Título: 	Polymorphism of the bovine calpain gene by PCR-RFLP analysis in specialised European, Zebuin and Brazilian naturalized breeds.	
Ano de publicação: 	2003	
Fonte/Imprenta: 	In: CONGRESO IBEROAMERICANO DE RAZAS CRIOLLAS, 6.; SIMPOSIO IBEROAMERICANO SOBRE CONSERVACION Y UTILIZACION DE RECURSOS ZOOGENETICOS, 4., 2003, Recife. Resumenes de Ponencias y Comunicaciones. Recife: FIRC / CYTED, 2003. nao paginado.	
Idioma: 	Inglês	
Conteúdo: 	The variability at the bovine m-calpain regulatory subunit (30kDa) gene were studied with PCR-RFLP analysis using the restriction endonuclease Hhal. 253 animals of meat specialised European breeds (Hereford, Aberdeen Angus) and milk (Friesian), as well as zebuin breeds (Gyr, Guzera, Nellore) and Brazilian naturalized breeds (Caracu, Junqueira, Pantaneira) were characterized. Three genotypes were identified, assigned AA, AB and BB, whose frequencies were 0.733, 0.2667 and 0 for Hereford, 0.4134, 0.3793 and 0.2073 for Aberdeen Angus, 0, 0.5454 and 0.4546 for Friesian, 0, 0.0952 and 0.9048 for Gyr, 0, 0.1622 and 0.8378 for Guzera, 0, 0.2432 and 0.,7568 for Nellore, 0.150, 0.625 and 0.225 for Caracu, 0, 1 and 0 for Junqueira and 0.1250, 0.6250 and 0.250 for Pantaneira breeds, respectively. The two alleles identified in study were assigned CAPN2 A e CAPN2 B. The first one was more frequent in specialised european breeds to meat, whose average frequency was 73.50%. The means of the frequencies to the CAPN2 A allele were 46.67 and 8,34% at the Brazilian naturalized and zebuin breeds. On the basis of the evidences in the literature and the results obtained in the present study, was formulated a hypothesis of a possible relation of CAPN2 A allele with meat tenderization, that will be investigated in samples of meats, from which related characteristics of quality of meat were evaluated. The results obtained are promising, once they demonstrate the participation of CAPN2 A allele in the zebuin populations and Brazilian naturalized breeds. This allows to affirm that the potential of our zebuinas breeds for production of quality meat, taking into account the consumer requirements. In case that the relation between CAPN2 A allele with meat tenderiztion is confirmed, it will be able to value for extinguishing of threatened genetic resources. This as the case for Pantaneiro and other Brazilian naturalized breeds, as well as for the Creoles on the South America and it an excellent aspect to not only demonstrate the importance of its conservation under point of view of extinguishing threatened genetic resources but also to divulgue the potential of this genetic material, adding economic values for national breeds bovine and Creole of the South America.	
Palavras-Chave: 	Bovine calpain gene; PCR-RFLP analysis.	
Thesagro: 	Bovino; Polimorfismo.	
Thesaurus Nal: 	polymorphism.	
Categoria do assunto: 	--	
Registro original: 	Embrapa Pantanal (CPAP)	
Exemplares: 	CPAP - (53781-1) - UPC - SP - -- - SP16516 -  - 16516-7 - DIS