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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
05/09/2006 |
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Data da última atualização: |
06/05/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
LEMES, M. R.; BRONDANI, R. P. V.; GRATTAPAGLIA, D. |
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Afiliação: |
MARISTERRA R. LMES, INPA; ROSANA PEREIRA VIANELLO, CNPAF; DARIO GRATTAPAGLIA, Cenargen. |
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Título: |
Multiplexed systems of microsatellite markers for genetic analysis of Mahogany, Swietenia macrophylla King (Meliaceae), a threatened neotropical timber species. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
The Journal of Heredity, v. 93, n. 4, p. 287-291, July 2002. |
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DOI: |
https://doi.org/10.1093/jhered/93.4.287 |
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Idioma: |
Inglês |
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Conteúdo: |
Mahogany (Swietenia macrophylla King [Meliaceae]) is the most valuable hardwood species in the neotropics. Its conservation status has been the subject of increasing concern due to overexploitation and habitat destruction. In this work we report the development and characterization of 10 highly variable microsatellite loci for S. macrophylla. Twenty-nine percent of the 126 sequenced mahogany clones yielded useful microsatellite loci. Three high-throughput genotyping systems were developed based on polymerase chain reaction (PCR) multiplexing of these mahogany loci. We identified a total of 158 alleles in 121 adult individuals of S. macrophylla, with an average of 15.8 alleles (range 11–25) per locus. All loci showed Mendelian inheritance in open-pollinated half-sib families. The mean expected heterozygosity was 0.84 and the mean observed heterozygosity was 0.73. The combined probability of identity—the probability that two individuals selected at random from a population would have identical genotypes—was 7.0 × 10−15, and combined probability of paternity exclusion was 0.999998 overall loci. These microsatellite loci permit precise estimates of parameters such as gene flow, mating system, and paternity, thus providing important insights into the population genetics and conservation of S. macrophylla. |
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Thesagro: |
Marcador Molecular; Mogno; Swietenia Macrophylla. |
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Thesaurus NAL: |
Cytogenetic analysis; Genetics; Hardwood; Microsatellite repeats. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02146naa a2200241 a 4500
001 1214349
005 2022-05-06
008 2002 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1093/jhered/93.4.287$2DOI
100 1 $aLEMES, M. R.
245 $aMultiplexed systems of microsatellite markers for genetic analysis of Mahogany, Swietenia macrophylla King (Meliaceae), a threatened neotropical timber species.$h[electronic resource]
260 $c2002
520 $aMahogany (Swietenia macrophylla King [Meliaceae]) is the most valuable hardwood species in the neotropics. Its conservation status has been the subject of increasing concern due to overexploitation and habitat destruction. In this work we report the development and characterization of 10 highly variable microsatellite loci for S. macrophylla. Twenty-nine percent of the 126 sequenced mahogany clones yielded useful microsatellite loci. Three high-throughput genotyping systems were developed based on polymerase chain reaction (PCR) multiplexing of these mahogany loci. We identified a total of 158 alleles in 121 adult individuals of S. macrophylla, with an average of 15.8 alleles (range 11–25) per locus. All loci showed Mendelian inheritance in open-pollinated half-sib families. The mean expected heterozygosity was 0.84 and the mean observed heterozygosity was 0.73. The combined probability of identity—the probability that two individuals selected at random from a population would have identical genotypes—was 7.0 × 10−15, and combined probability of paternity exclusion was 0.999998 overall loci. These microsatellite loci permit precise estimates of parameters such as gene flow, mating system, and paternity, thus providing important insights into the population genetics and conservation of S. macrophylla.
650 $aCytogenetic analysis
650 $aGenetics
650 $aHardwood
650 $aMicrosatellite repeats
650 $aMarcador Molecular
650 $aMogno
650 $aSwietenia Macrophylla
700 1 $aBRONDANI, R. P. V.
700 1 $aGRATTAPAGLIA, D.
773 $tThe Journal of Heredity$gv. 93, n. 4, p. 287-291, July 2002.
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