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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
22/12/1999 |
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Data da última atualização: |
22/12/1999 |
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Autoria: |
POSADAS, D. A.; TANNUS, A.; PANEPUCCI, H.; CRESTANA, S. |
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Afiliação: |
Universidade Estadual de Ponta Grossa; EMBRAPA-CNPDIA; USP-IFSC. |
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Título: |
Magnetic resonance imaging as a non-invasive technique for investigating 3-D preferential flow occurring within stratified soil samples. |
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Ano de publicação: |
1996 |
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Fonte/Imprenta: |
Computers and Electronics in Agriculture, Amsterdam, v.14, n.4, p.255-267, Apr. 1996. |
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Idioma: |
Inglês |
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Conteúdo: |
The soil fingering phenomenon was investigated using magnetic resonance imaging (MRI) as an appropriate technique. A cubic 15x15x15-cm3 double-layer sand column was built in order to simulate the stratified soil. The column was imaged in a 500 Gaus NMR tomograph [IFQSC, USP, Sao Carlos (SP)]. After reaching the water steady-state flow several coronal, transverse, and sagittal images of the column were obtained. For each case seven slices, 1.8cm thick and separated by 2.0cm center to center, were collected providing three-dimensional information regardingto the number of fingers, their sizes and diameters. To study the fingering dynamics employing MRI concepts, another kind of experiment was performed by following only the water front (no image reconstruction), using spin-echo signals with phase encoding along vertical axis (gravitational direction), but with phase encoding gradients switched off. Sixteen acquisitions of seven transverses of 2cm thick slices, were performed in a 3-min experiment. After suitable treatment, the water front and the horizontal distribution at each measured time during the water infiltration process was obtained. Normalizing the signal intensities to the soil column dimensions and using computational graphic resources, made it possible to quantify the number of fingers and their spatial and temporal variability. The results elect MRI as a valuable tool for non-invasively investigating the dynamics of soil fingering phenomenon. |
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Palavras-Chave: |
Fingering; Fractal; Gravity force; Imaging; NMR tomography; Nuclear magnetic resonance; Spin echo signal. |
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Categoria do assunto: |
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Marc: |
LEADER 02221naa a2200241 a 4500
001 1026423
005 1999-12-22
008 1996 bl --- 0-- u #d
100 1 $aPOSADAS, D. A.
245 $aMagnetic resonance imaging as a non-invasive technique for investigating 3-D preferential flow occurring within stratified soil samples.
260 $c1996
520 $aThe soil fingering phenomenon was investigated using magnetic resonance imaging (MRI) as an appropriate technique. A cubic 15x15x15-cm3 double-layer sand column was built in order to simulate the stratified soil. The column was imaged in a 500 Gaus NMR tomograph [IFQSC, USP, Sao Carlos (SP)]. After reaching the water steady-state flow several coronal, transverse, and sagittal images of the column were obtained. For each case seven slices, 1.8cm thick and separated by 2.0cm center to center, were collected providing three-dimensional information regardingto the number of fingers, their sizes and diameters. To study the fingering dynamics employing MRI concepts, another kind of experiment was performed by following only the water front (no image reconstruction), using spin-echo signals with phase encoding along vertical axis (gravitational direction), but with phase encoding gradients switched off. Sixteen acquisitions of seven transverses of 2cm thick slices, were performed in a 3-min experiment. After suitable treatment, the water front and the horizontal distribution at each measured time during the water infiltration process was obtained. Normalizing the signal intensities to the soil column dimensions and using computational graphic resources, made it possible to quantify the number of fingers and their spatial and temporal variability. The results elect MRI as a valuable tool for non-invasively investigating the dynamics of soil fingering phenomenon.
653 $aFingering
653 $aFractal
653 $aGravity force
653 $aImaging
653 $aNMR tomography
653 $aNuclear magnetic resonance
653 $aSpin echo signal
700 1 $aTANNUS, A.
700 1 $aPANEPUCCI, H.
700 1 $aCRESTANA, S.
773 $tComputers and Electronics in Agriculture, Amsterdam$gv.14, n.4, p.255-267, Apr. 1996.
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Registro original: |
Embrapa Instrumentação (CNPDIA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Caprinos e Ovinos. Para informações adicionais entre em contato com cnpc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
28/11/2013 |
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Data da última atualização: |
22/06/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
SOUZA, K. C. de; PINHEIRO, R. R.; SANTOS, D. O.; BRITO, R. L. L. de; RODRIGUES, A. de S.; SIDER, L. H.; PAULA, N. R. O.; AVILAB, A. A.; CARDOSO, J. de F. S.; ANDRIOLI, A. |
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Afiliação: |
Kelma Costa de Souza, Pós-graduação - Universidade Estadual Vale do Acaraú (UVA) - Sobral, CE, Brasil.; RAYMUNDO RIZALDO PINHEIRO, CNPC; DIONES OLIVEIRA SANTOS, CNPC; Roberta Lomonte Lemos de Brito; Apoliana de Souza Rodrigues; LUCIA HELENA SIDER, CNPC; Ney Rômulo Oliveira Paula; Amanda Aragão Avila, UVA - Sobral, CE, Brasil; Janaina de Fátima Saraiva Cardoso, Universidade Federal do Piauí (UFPI) - Teresina, PI, Brasil; ALICE ANDRIOLI, CNPC. |
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Título: |
Transmission of the caprine arthritis-encephalitis virus through artificial insemination. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Small Ruminant Research, v. 109, n. 2/3, p. 193-198, Jan. 2013. |
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DOI: |
https://doi.org/10.1016/j.smallrumres.2012.07.031 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: The objective of present study was to evaluate the transmissibility of the caprine arthritis-encephalitis virus (CAEV) through artificial insemination (AI), and to assess the influence of viral load on this probable transmission. It also aims to verify whether the inflammatory process caused by the use of intravaginal sponges would facilitate virus entry in the female reproductive tract. For this purpose, 30 undefined breed goats were used, all serologically negative for CAEV. One Anglo-Nubian buck, also seronegative, was used to artificially inseminate females in this study. His semen was contaminated with the standard CAEV-Cork virus strain, with two distinct infective titres, one 106 TCID50/mL, for high viral load (HVL), and another of 102 TCID50/mL, for low viral load (LVL). Females had estrus synchronised by using two protocols, intravaginal sponges in Group 1 (G1, N = 15) and auricular subcutaneous implants in Group 2 (G2, N = 15). For inseminations, the goats were divided into three groups of 10 animals each. One group was inseminated with HVL, another with LVL and the third with semen from the same virus-free buck, as a negative control. The experiment was conducted in accordance to the ethical principles for animal experimentation. Statistical analyses were performed by the chi-square test (P < 0.05). Thirty days after insemination, the experimental infection was confirmed, when 12 out of the 20 (60%) inseminated goats had seroconverted. Sixty days after insemination, all females from the HVL and LVL groups presented anti-CAEV antibodies. There was no statistical difference (P > 0.05) among groups regarding viral loads nor between the two estrus synchronisation protocols. Goats from the control group remained seronegative throughout the experiment (12 months). Concerning reproductive parameters, no difference was found between the control group and the infected groups. Based on these results, it is possible to conclude that the virus can be transmitted through artificial insemination with infected semen. Therefore, the venereal route is a potential route of infection. MenosAbstract: The objective of present study was to evaluate the transmissibility of the caprine arthritis-encephalitis virus (CAEV) through artificial insemination (AI), and to assess the influence of viral load on this probable transmission. It also aims to verify whether the inflammatory process caused by the use of intravaginal sponges would facilitate virus entry in the female reproductive tract. For this purpose, 30 undefined breed goats were used, all serologically negative for CAEV. One Anglo-Nubian buck, also seronegative, was used to artificially inseminate females in this study. His semen was contaminated with the standard CAEV-Cork virus strain, with two distinct infective titres, one 106 TCID50/mL, for high viral load (HVL), and another of 102 TCID50/mL, for low viral load (LVL). Females had estrus synchronised by using two protocols, intravaginal sponges in Group 1 (G1, N = 15) and auricular subcutaneous implants in Group 2 (G2, N = 15). For inseminations, the goats were divided into three groups of 10 animals each. One group was inseminated with HVL, another with LVL and the third with semen from the same virus-free buck, as a negative control. The experiment was conducted in accordance to the ethical principles for animal experimentation. Statistical analyses were performed by the chi-square test (P < 0.05). Thirty days after insemination, the experimental infection was confirmed, when 12 out of the 20 (60%) inseminated goats had seroconverted. Sixty days after i... Mostrar Tudo |
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Palavras-Chave: |
Artrite encefalite caprina; CAE; CAEV; Caprine arthritis encephalit virus; Carga viral; Transmissions. |
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Thesagro: |
Artrite; Caprino; Doença animal; Inseminação artificial; Sêmen; Transmissão de doença. |
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Thesaurus NAL: |
Animal diseases; Artificial insemination; Disease transmission; Goats. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 03396naa a2200433 a 4500
001 1972333
005 2020-06-22
008 2013 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.smallrumres.2012.07.031$2DOI
100 1 $aSOUZA, K. C. de
245 $aTransmission of the caprine arthritis-encephalitis virus through artificial insemination.$h[electronic resource]
260 $c2013
520 $aAbstract: The objective of present study was to evaluate the transmissibility of the caprine arthritis-encephalitis virus (CAEV) through artificial insemination (AI), and to assess the influence of viral load on this probable transmission. It also aims to verify whether the inflammatory process caused by the use of intravaginal sponges would facilitate virus entry in the female reproductive tract. For this purpose, 30 undefined breed goats were used, all serologically negative for CAEV. One Anglo-Nubian buck, also seronegative, was used to artificially inseminate females in this study. His semen was contaminated with the standard CAEV-Cork virus strain, with two distinct infective titres, one 106 TCID50/mL, for high viral load (HVL), and another of 102 TCID50/mL, for low viral load (LVL). Females had estrus synchronised by using two protocols, intravaginal sponges in Group 1 (G1, N = 15) and auricular subcutaneous implants in Group 2 (G2, N = 15). For inseminations, the goats were divided into three groups of 10 animals each. One group was inseminated with HVL, another with LVL and the third with semen from the same virus-free buck, as a negative control. The experiment was conducted in accordance to the ethical principles for animal experimentation. Statistical analyses were performed by the chi-square test (P < 0.05). Thirty days after insemination, the experimental infection was confirmed, when 12 out of the 20 (60%) inseminated goats had seroconverted. Sixty days after insemination, all females from the HVL and LVL groups presented anti-CAEV antibodies. There was no statistical difference (P > 0.05) among groups regarding viral loads nor between the two estrus synchronisation protocols. Goats from the control group remained seronegative throughout the experiment (12 months). Concerning reproductive parameters, no difference was found between the control group and the infected groups. Based on these results, it is possible to conclude that the virus can be transmitted through artificial insemination with infected semen. Therefore, the venereal route is a potential route of infection.
650 $aAnimal diseases
650 $aArtificial insemination
650 $aDisease transmission
650 $aGoats
650 $aArtrite
650 $aCaprino
650 $aDoença animal
650 $aInseminação artificial
650 $aSêmen
650 $aTransmissão de doença
653 $aArtrite encefalite caprina
653 $aCAE
653 $aCAEV
653 $aCaprine arthritis encephalit virus
653 $aCarga viral
653 $aTransmissions
700 1 $aPINHEIRO, R. R.
700 1 $aSANTOS, D. O.
700 1 $aBRITO, R. L. L. de
700 1 $aRODRIGUES, A. de S.
700 1 $aSIDER, L. H.
700 1 $aPAULA, N. R. O.
700 1 $aAVILAB, A. A.
700 1 $aCARDOSO, J. de F. S.
700 1 $aANDRIOLI, A.
773 $tSmall Ruminant Research$gv. 109, n. 2/3, p. 193-198, Jan. 2013.
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Embrapa Caprinos e Ovinos (CNPC) |
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