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| Registros recuperados : 265 | |
| 81. |  | FONSECA, J. F. da; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. M. G.; OLIVEIRA, M. E. F.; BRANDÃO, F. Z.; VIANA, J. H. M. Freezing goat embryos at different developmental stages and quality using ethylene glycol and a slow cooling rate. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 70, n. 5, p. 1489-1496, 2018.| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Recursos Genéticos e Biotecnologia. |
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| 82. | | TABOSA, T. G. B.; FONSECA, J. F. da; FIGUEIRA, L. M.; TEIXEIRA, T. A.; CARVALHEIRA, L. R.; BALARO, M. F. A.; BRANDÃO, F. Z.; NOGUEIRA, L. A. G. GnRH use in different times on estrus synchronization and ovulation in Santa Inês ewes. Animal Reproduction, v. 11, n. 3, Jul./Sept. 2014. Abstract A117. Proceedings of the 28th Annual Meeting of The Brazilian Embryo Technology Society - SBTE, Natal, RN, Brasil, ago. 2014.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 83. | | FONSECA, J. F. da; BATISTA, R. I. T. P.; TREVIZAN, J. T.; SOUZA-FABJAN, J. M. G.; BRANDÃO, F. Z.; CAMARGO, L. S. de A. Goat incubator: can bovine oocytes be matured in the uterine horn of a goat? Semina: Ciências Agrárias, Londrina, v. 40, n. 6, supp. 3, p. 3789-3796, 2019.| Biblioteca(s): Embrapa Gado de Leite. |
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| 84. | | BATISTA, R. I. T. P.; CAMARGO, L. S. de A.; SOUZA FABJAN, J. M. G.; PRATES, J. F.; TREVIZAN, J. T.; BRANDÃO, F. Z.; FONSECA, J. F. da. Goat incubator: the doe as a life incubator of bovine oocytes - first step. Animal Reprodroduction, v. 14, n. 3, p. 738, Jul./Sept. 2017. Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 85. | | MAIA, A. L. R. S.; BRANDÃO, F. Z.; SOUZA-FABJAN, J. M. G.; ARAÚJO, M. C. C.; SIQUEIRA, L. G. B.; FACO, O.; FONSECA, J. F. da. Hydrosalpinx in dairy goats: occurrence, ultrasound diagnosis, macro- and microscopic characterization. Small Ruminant Research, v. 160, p. 5-11, 2018.| Biblioteca(s): Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
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| 86. | | FÉRES, L. F. R.; BRANDÃO, F. Z.; SANTOS, L. L.; CAMARGO, L. S. de A.; TORRES, H. A. L.; RAIDAN, F. S. S.; VIANA, J. H. M. Inbreeding and its relationship with oocyte production in the Gir (Bos indicus) breed: preliminary results. Animal Reproduction, v. 15, n. 3, p. 393, 2018. Edição dos proceedings do 32º Annual Meeting of the Brazilian Embryo Technology Society, Florianópolis, 2018.| Biblioteca(s): Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia. |
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| 87. | | MONTEIRO NETTO, M.; BALARO, M. F. A.; ESPÍRITO SANTO, C. G. do; COSENTINO, I. O.; BRANDÃO, F. Z.; FONSECA, J. F. da. Indução e sincronização do estro por programa de luz e d-cloprostenol em cabras leiteiras na contra-estação reprodutiva. Revista Brasileira de Reprodução Animal, v. 43, n. 2, p. 539, abr./jun. 2019. Edição dos resumos do XXIII Congresso Brasileiro de Reprodução Animal, Gramado, RS, Brasil, 15 a 17 de maio 2019.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 88. | | TEIXEIRA, T. A.; FONSECA, J. F. da; CARVALHEIRA, L. R.; SARZEDAS, A. C.; CONSALTER, A.; DELGADO, K. F.; BRANDÃO, F. Z.; VASCONCELOS, C. O. P. Influence or duration of treatment with medroxyprogesterone acetate sponge on reproductive parameters of Santa Inês ewes subjected to estrus synchronization. Animal Reproduction, v. 11, n. 3, Jul./Sept. 2014. Abstract A116. Proceedings of the 28th Annual Meeting of The Brazilian Embryo Technology Society - SBTE, Natal, RN, Brasil, ago. 2014.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 89. | | OLIVARES, C. C. S.; SOUZA-FABJAN, J. M. G.; PENEIRAS, A. B. V.; BALARO, M. F. A.; FREITAS, V. J. F.; FONSECA, J. F. da; BRANDÃO, F. Z. The influence of different methods of frozen-thawed ovine spermatozoa selection on sperm capacitation and viability after incubation. Animal Reproduction, v. 12, n. 3, p. 554, Jul./Sept. 2015. Proceedings of the 29th Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Gramado, RS, Brazil, August 20 to 23, 2015. Abstracts.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 90. | | TEIXEIRA, T. A.; FONSECA, J. F. da; CARVALHEIRA, L. R.; SARZEDAS, A. C.; CONSALTER, A.; DELGADO, K. F.; BRANDÃO, F. Z.; VASCONCELOS, C. O. P. Influence of duration of treatment with medroxyprogesterone acetate sponge on reproductive parameters of Santa Inês ewes subjected to estrus synchronization. Animal Reproduction, v. 11, n. 3, p. 396, Jul./Sept. 2014. Abstract A117. Proceedings of the 28th Annual Meeting of the Brazilian Embryo Technology Society (SBTE), August, 2014, Natal, RN, Brazil. Abstracts.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 91. | | CAMPOS, M. M.; SANTOS, G. B.; BRANDÃO, F. Z.; CARVALHO, B. C. de; MACHADO, F. S.; VARGAS, M. W.; VARAGO, F. C.; TOMICH, T. R. Milk production and composition in crossbred primiparous dairy cows fed different energy diets in the first 56 days of lactation. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA, 51., 2014, Barra dos Coqueiros. A produção animal frente às mudanças climáticas e tecnológicas: resumos. Barra dos coqueiros: Sociedade Brasileira de Zootecnia, 2014.| Biblioteca(s): Embrapa Gado de Leite. |
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| 92. | | SANTOS, G. B. DOS; BRANDÃO, F. Z.; RIBEIRO, L. DOS S.; FERREIRA, A. L.; CAMPOS, M. M.; MACHADO, F. S.; CARVALHO, B. C. de. Milk production and composition, food consumption, and energy balance of postpartum crossbred Holstein-Gir dairy cows fed two diets of different energy levels. Tropical Animal Health and Production, v. 51, p. 65-71, 2019.| Biblioteca(s): Embrapa Gado de Leite. |
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| 93. | | OLIVEIRA, J. S. K.; FONSECA, J. F. da; ESTEVES, L. V.; MARTINS, G.; PENNA, B.; LILENBAUM, W.; FIGUEIRA, L. M.; BRANDÃO, F. Z. Mudanças na biota vaginal de cabras submetidas a protocolo de indução de estro sincronizado. In: CONGRESSO BRASILEIRO DE REPRODUÇÃO ANIMAL, 19., 2011, Recife. Anais... Belo Horizonte: CBRA, 2011. p. 227. 1 CD-ROM.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 95. | | ARASHIRO, E. K. N.; LIBONATI, H.; SANTOS, G. B. dos; NOGUEIRA, L. A. G.; SOUZA, G. N. de; LILENBAUM, W.; BRANDÃO, F. Z. Repetition of estrus is the most frequent reproductive problem after breeding in dairy cattle from Rio de Janeiro, Brazil. Ciência Rural, v. 47, n. 7, e20161092, 2017.| Biblioteca(s): Embrapa Gado de Leite. |
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| 96. | | FONSECA, J. F. da; ALVIM, G. P.; SOUZA-FABJAN, J. M. G.; OLIVEIRA, M. E. F.; BRAIR, V. L.; BRANDÃO, F. Z.; FACO, O. Reproductive features and use of an anti-inflammatory drug in estrus-induced dairy goats artificially inseminated in a standing position with cervix immobilization. Reproductive Biology, Olsztyn, v. 17, n. 3, p. 268-273, Sep. 2017.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 97. | | MAIA, A. L. R. S.; BRANDÃO, F. Z.; SOUZA-FABJAN, J. M. G.; BALARO, M. F. A.; OLIVEIRA, M. E. F.; FACO, O.; FONSECA, J. F. da. Reproductive parameters of dairy goats after receiving two doses of d-cloprostenol at different intervals. Animal Reproduction Science, v. 1981, p. 16-23, June, 2017.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 98. | | ESTEVES, L. V.; BRANDÃO, F. Z.; CRUZ, R. C.; SOUZA, J. M. G.; OBA, E.; FACO, O.; FONSECA, J. F. da. Reproductive parameters of dairy goats submitted to estrus synchronization with prostaglandin F2alfa associated or not to hCG at estrous onset. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 65, n. 6, p. 1585-1592, 2013.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 99. | | BALARO, M. F. A.; BRANDÃO, F. Z.; PENEIRAS, A. B. V.; OBA, E.; FONSECA, J. F. da; ALMOSNY, N. R. P.; CARDOSO, E. da C. Reproductive performance, metabolic and hormonal profiles of Santa Inês ewes in winter and summer under tropical conditions. Tropical Animal Health and Production, v. 47, N. 3, p. 627-631, Mar. 2015.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 100. | | BALARO, M. F. A.; MELLO, S. G. V. de; SANTOS, A. da S.; CAVALCANTI, L. M.; ALMOSNY, N. R. P.; FONSECA, J. F. da; BRANDÃO, F. Z. Reproductive seasonality in Saanen goats kept under tropical conditions. Tropical Animal Health and Production, v. 51, n. 2, p. 345-353, Feb. 2019.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| Registros recuperados : 265 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
24/08/2017 |
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Data da última atualização: |
23/09/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
BATISTA, R. I. T. P.; CAMARGO, L. S. de A.; SOUZA FABJAN, J. M. G.; PRATES, J. F.; TREVIZAN, J. T.; BRANDÃO, F. Z.; FONSECA, J. F. da. |
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Afiliação: |
Ribrio Ivan Tavares Pereira Batista; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; Joanna Maria Gonçalves de Souza Fabjan, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brazil; Jader Forquim Prates; Juliane Teramachi Trevizan; Felipe Zandonadi Brandao; JEFERSON FERREIRA DA FONSECA, CNPC. |
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Título: |
Goat incubator: the doe as a life incubator of bovine oocytes - first step. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Animal Reprodroduction, v. 14, n. 3, p. 738, Jul./Sept. 2017. |
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Idioma: |
Inglês |
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Notas: |
Proceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts. |
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Conteúdo: |
Despite significant improvements in the in vitro production of cattle embryos, the suboptimal in vitro culture environment still limits the embryo quality and production. Techniques that associate the advantages of in vivo and in vitro systems, such as intrafollicular transfer of immature oocytes, have been proposed mainly to increase the embryo quality. In this context, we tried to use a goat as live incubator and associated nonsurgical embryo transfer techniques in small ruminants to perform ex situ (in vivo) maturation of bovine oocytes. For this, immature bovine cumulus-oocyte complexes (COCs) of grade 1 and 2 were randomly distributed into two groups for in vitro (IVM; n = 38) and ex situ (ESM; n = 40) maturation. The IVM was performed for a period of 24 h in TCM-199 medium (Gibco Life Technologies, Inc., Grand Island, NY, USA) supplemented with 20 mg/mL of FSH (Pluset, Calier, Barcelona, Spain), 0.36 mM sodium pyruvate (Sigma Chemical, St. Louis, MO, USA), 10 mM sodium bicarbonate (Sigma Chemical, St. Louis, MO, USA) and 50 mg/mL streptomycin/penicillin (Sigma Chemical, St. Louis, MO, USA) at 38.8 ºC in an atmosphere of 5% CO2 in air with maximum humidity. For ESM, a pre-synchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apice by transcervical route (Fonseca et al., 2014 Arq. Bras. Med.vet. Zootec) and 24 h after the procedure the structures were retrieved by the uterine flushing (Fonseca et al., 2013 Small Rumin Res). For analysis of the nuclear maturation rate and lipid quantification, the oocytes were denuded (0.1% hyaluronidase), fixed (4% paraformaldehyde) and stained with 10 ?g/mL Hoechst 33342 and 10 ?g/mL Nile Red (Molecular Probes, Inc., Eugene, OR, USA) dissolved in physiological saline (0.9% NaCl) with 1mg/mL polyvinylpyrrolidone. Oocytes displaying metaphase II plate were considered matured. The lipid amount was inferred by measuring the fluorescence intensity using the ImageJ program and fluorescence intensity were compared by Student's t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for ESM and IVM groups, respectively. In vitro-matured oocytes contained more lipid droplets, expressed as a higher (p < 0.05) amount of emitted fluorescence light (858 ± 73 arbitrary fluorescence units) than ex situ-matured oocytes (550 ± 64 arbitrary fluorescence units). This is the first report associating nonsurgical embryo transfer techniques with goat as live incubator for maturation of bovine oocytes. We conclude that transcervical transfer of bovine oocytes to uterine goat may be an alternative to in vitro maturation aiming the reduction of lipids without compromising nuclear maturation. Further studies are required to improve the oocyte recovery rate. MenosDespite significant improvements in the in vitro production of cattle embryos, the suboptimal in vitro culture environment still limits the embryo quality and production. Techniques that associate the advantages of in vivo and in vitro systems, such as intrafollicular transfer of immature oocytes, have been proposed mainly to increase the embryo quality. In this context, we tried to use a goat as live incubator and associated nonsurgical embryo transfer techniques in small ruminants to perform ex situ (in vivo) maturation of bovine oocytes. For this, immature bovine cumulus-oocyte complexes (COCs) of grade 1 and 2 were randomly distributed into two groups for in vitro (IVM; n = 38) and ex situ (ESM; n = 40) maturation. The IVM was performed for a period of 24 h in TCM-199 medium (Gibco Life Technologies, Inc., Grand Island, NY, USA) supplemented with 20 mg/mL of FSH (Pluset, Calier, Barcelona, Spain), 0.36 mM sodium pyruvate (Sigma Chemical, St. Louis, MO, USA), 10 mM sodium bicarbonate (Sigma Chemical, St. Louis, MO, USA) and 50 mg/mL streptomycin/penicillin (Sigma Chemical, St. Louis, MO, USA) at 38.8 ºC in an atmosphere of 5% CO2 in air with maximum humidity. For ESM, a pre-synchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apice by transcervical route (Fonseca et al., 2014 Arq. Bras. Med.vet. Zootec) and 24 h after the procedure the structures were retrieved by the uterine flushing (Fonseca et al., 2013 Small Rumin Res). For analy... Mostrar Tudo |
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Palavras-Chave: |
Embryo Technology; Maturation; Non-surgical collection; Tecnologia do embrião. |
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Thesagro: |
Cultura In Vitro; Maturação artificial; Ovelha; Ovino; Reprodução animal. |
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Thesaurus NAL: |
Ewes; In vitro culture; In vitro fertilization; Ova; Reproduction; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/163010/1/cnpc-2017-Goat.pdf
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Marc: |
LEADER 04078nam a2200373 a 4500
001 2074430
005 2019-09-23
008 2017 bl uuuu u00u1 u #d
100 1 $aBATISTA, R. I. T. P.
245 $aGoat incubator$bthe doe as a life incubator of bovine oocytes - first step.$h[electronic resource]
260 $aAnimal Reprodroduction, v. 14, n. 3, p. 738, Jul./Sept. 2017.$c2017
500 $aProceedings of the 31st Annual Meeting of the Brazilian Embryo Technology Society (SBTE); Cabo de Santo Agostinho, PE, Brazil, August 17th to 19th, 2017. Abstracts.
520 $aDespite significant improvements in the in vitro production of cattle embryos, the suboptimal in vitro culture environment still limits the embryo quality and production. Techniques that associate the advantages of in vivo and in vitro systems, such as intrafollicular transfer of immature oocytes, have been proposed mainly to increase the embryo quality. In this context, we tried to use a goat as live incubator and associated nonsurgical embryo transfer techniques in small ruminants to perform ex situ (in vivo) maturation of bovine oocytes. For this, immature bovine cumulus-oocyte complexes (COCs) of grade 1 and 2 were randomly distributed into two groups for in vitro (IVM; n = 38) and ex situ (ESM; n = 40) maturation. The IVM was performed for a period of 24 h in TCM-199 medium (Gibco Life Technologies, Inc., Grand Island, NY, USA) supplemented with 20 mg/mL of FSH (Pluset, Calier, Barcelona, Spain), 0.36 mM sodium pyruvate (Sigma Chemical, St. Louis, MO, USA), 10 mM sodium bicarbonate (Sigma Chemical, St. Louis, MO, USA) and 50 mg/mL streptomycin/penicillin (Sigma Chemical, St. Louis, MO, USA) at 38.8 ºC in an atmosphere of 5% CO2 in air with maximum humidity. For ESM, a pre-synchronized nulliparous goat (12 months old) received 40 immature COCs in the uterine horn apice by transcervical route (Fonseca et al., 2014 Arq. Bras. Med.vet. Zootec) and 24 h after the procedure the structures were retrieved by the uterine flushing (Fonseca et al., 2013 Small Rumin Res). For analysis of the nuclear maturation rate and lipid quantification, the oocytes were denuded (0.1% hyaluronidase), fixed (4% paraformaldehyde) and stained with 10 ?g/mL Hoechst 33342 and 10 ?g/mL Nile Red (Molecular Probes, Inc., Eugene, OR, USA) dissolved in physiological saline (0.9% NaCl) with 1mg/mL polyvinylpyrrolidone. Oocytes displaying metaphase II plate were considered matured. The lipid amount was inferred by measuring the fluorescence intensity using the ImageJ program and fluorescence intensity were compared by Student's t-test. Forty-seven percent of the structures were recovered after uterine flushing (19/40). The nuclear maturation rate was 94.5% (18/19) and 81.6% (31/38) for ESM and IVM groups, respectively. In vitro-matured oocytes contained more lipid droplets, expressed as a higher (p < 0.05) amount of emitted fluorescence light (858 ± 73 arbitrary fluorescence units) than ex situ-matured oocytes (550 ± 64 arbitrary fluorescence units). This is the first report associating nonsurgical embryo transfer techniques with goat as live incubator for maturation of bovine oocytes. We conclude that transcervical transfer of bovine oocytes to uterine goat may be an alternative to in vitro maturation aiming the reduction of lipids without compromising nuclear maturation. Further studies are required to improve the oocyte recovery rate.
650 $aEwes
650 $aIn vitro culture
650 $aIn vitro fertilization
650 $aOva
650 $aReproduction
650 $aSheep
650 $aCultura In Vitro
650 $aMaturação artificial
650 $aOvelha
650 $aOvino
650 $aReprodução animal
653 $aEmbryo Technology
653 $aMaturation
653 $aNon-surgical collection
653 $aTecnologia do embrião
700 1 $aCAMARGO, L. S. de A.
700 1 $aSOUZA FABJAN, J. M. G.
700 1 $aPRATES, J. F.
700 1 $aTREVIZAN, J. T.
700 1 $aBRANDÃO, F. Z.
700 1 $aFONSECA, J. F. da
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