Data/hora: 16/06/2024 22:56:54

Biblioteca(s): | Embrapa Clima Temperado; Embrapa Trigo.
Data corrente: | 08/03/2024
Data da última atualização: | 08/03/2024
Autoria: | CASASSOLA, A.; EREFUL, N. C.; ZANELLA, C. M.; PANDEY, P.; BRAMMER, S. P.; CHAVES, M. S.; MARTINELLI, J. A.; BOYD, L. A.
Afiliação: | ALICE CASASSOLA; NELZO C. EREFUL; CAMILA M. ZANELLA; POOJA PANDEY; SANDRA PATUSSI BRAMMER, CNPT; MARCIA SOARES CHAVES, CPACT; JOSÉ A. MARTINELLI, UNIVERSIDADE FEDERAL DO RIO GRANDE DO SUL; LESLEY A. BOYD.
Título: | Transcriptional profiling identifies the early responses to Puccinia triticina infection in the adult plant leaf rust resistant wheat variety Toropi.
Ano de publicação: | 2024
Fonte/Imprenta: | Plant Pathology, 2024.
DOI: | 10.1111/ppa.13865
Idioma: | Inglês
Notas: | Online first. Early view.
Conteúdo: | Leaf rust, caused by Puccinia triticina (Pt), is a major disease of wheat and a significant problem for wheat production in Brazil. The Brazilian variety Toropi, released in 1965, has maintained high levels of field, adult plant resistance (APR) to leaf rust across global  locations,  while  microscopic  studies  have  indicated  prehaustorial  resistance  mechanisms.  Analyses  of  gene  expression  in  flag  leaves  of  Toropi,  during  the  early  stages of Pt infection, were undertaken to explore the mechanisms behind the APR in Toropi. Differential expression of wheat genes was undertaken, comparing Pt-  to mock-    inoculated and Pt-  to Pt-    inoculated time points. Analysis of gene expression indicated a strong response to Pt, which was fully active by 6 h after inoculation (hai). More genes were downregulated than upregulated, particularly at 6 and 12 hai. Gene Ontology enrichment analysis indicated a shutting down of RNA and protein synthe-sis  and  an  early  effect  on  photosynthesis,  with  disruption  of  the  electron  transfer  chain. Analyses of upregulated genes identified genes involved in ATP-binding and protein kinase activity at 6 hai, supporting a rapid metabolic response to Pt infection. A general upregulation of genes involved in transport and metabolism indicated the need to relocate protein and organic- based resources. Alignment of differentially ex-pressed genes with the genomic regions defining four leaf rust APR quantitative trait loci  (QTLs)  in  Toropi  identified  candidate  resistance  genes,  including  a  sugar  trans-porter, a receptor kinase and a seven-transmembrane MLO family protein. In addition, 60 Pt genes were identified, 11 being annotated as potential effector proteins.
Palavras-Chave: | Puccinia triticina.
Thesagro: | Ferrugem; Resistência; Trigo.
Categoria do assunto: | --
Registro original: | Embrapa Trigo (CNPT)

Exemplares:

CPACT - (22806-1) - UPC - AP - DD -  -  -  - DIS
CNPT - (45631-1) - UPC - AP - DD -  -  -  - DIS