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Registros recuperados : 10 | |
3. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | FERREIRA, I. M.; VIEIRA, G. S.; BRAGA, T. F.; SILVA, T. C. F.; FRANCO, M. M.; ANTUNES, R. C. Allelic and phenotypic frequencies of point mutations (SNPs) in the HFAB, DEGAT1, LEPR, MC4R, SREBF1 and GH genes in Large White matrices. In: BRAZILIAN INTERNATIONAL CONGRESS OF GENETICS, 2017, Águas de Lindóia, 12 a 15 set. 2017. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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6. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | BRAGA, T. F.; MARQUES, M. G.; SOUZA, A. P.; ALBRING, D.; BORGES, N. A.; DODE, M. A. N.; FRANCO, M. M. Characterization of gene expression profile in immature and in vitro matured pig oocytes. In: CONGRESSO BRASILEIRO DE GENÉTICA, 62., 2016, Caxambu. Resumos... Ribeirão Preto: Sociedade Brasileira de Genética, 2016. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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7. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | LEME, L. de O.; DUFORT, I.; SPRICIGO, J. F. W.; BRAGA, T. F.; SIRARD, M.; FRANCO, M. M.; DODE, M. A. N. Effect of vitrification using the Cryotop method on the gene expression profile of in vitro-produced bovine embryos. Theriogenology, v. 85, 724-733, 2016. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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8. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | FERREIRA, I. M.; BRAGA, T. F.; GUIMARÃES, E. C.; DURVAL, M. C.; MENDES, L. B.; SOARES, J. S.; MATARIM, D. L.; KRUGER, B. C.; FRANCO, M. M.; ANTUNES, R. C. A DdeI polymorphism in the growth hormone gene is associated with higher lean meat yield and pH 45 min in postmortem pigs. Genetics and Molecular Research, v. 18, n. 2, article gmr18257, 2019. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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9. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | SILVA, T. C. F.; DODE, M. A. N.; BRAGA, T. F.; MARQUES, M. G.; VARGAS, L. N.; FARIA, O. A. C. de; SOUZA, A. P. de; ALBRING, D.; CAETANO, A. R.; FRANCO, M. M. Cumulus-oocyte complexes from sows show differences in lipid metabolism compared to cumulus-oocyte complexes from prepubertal gilts during in vitro maturation. Molecular Reproduction & Development, v. 90, n. 5, p. 323-335. 2023. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia; Embrapa Suínos e Aves. |
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10. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | BRAGA, T. F.; SILVA, T. C. F.; MARQUES, M. G.; SOUZA, A. P. de; ALBRING, D.; SILVA, L. P.; CAETANO, A. R.; DODE, M. A. N.; FRANCO, M. M. The dynamics of gene expression, lipid composition and DNA methylation reprogramming are different during in vitro maturation of pig oocytes obtained from prepubertal gilts and cycling sows. Reproduction in Domestic Animals, v. 54, n. 9, p. 1217-1229, 2019. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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Registros recuperados : 10 | |
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
06/01/2016 |
Data da última atualização: |
20/03/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 2 |
Autoria: |
LEME, L. de O.; DUFORT, I.; SPRICIGO, J. F. W.; BRAGA, T. F.; SIRARD, M.; FRANCO, M. M.; DODE, M. A. N. |
Afiliação: |
LIGIANE DE OLIVEIRA LEME, UnB; ISABELLE DUFORT, Université Laval, Québec City, Quebec, Canada; JOSÉ FELIPE WARMLING SPRICIGO, UnB; THIAGO FELIPE BRAGA, UnB; MARC-ANDRÉ SIRARD, Université Laval, Québec City, Quebec, Canada; MAURICIO MACHAIM FRANCO, CENARGEN; MARGOT ALVES NUNES DODE, CENARGEN. |
Título: |
Effect of vitrification using the Cryotop method on the gene expression profile of in vitro-produced bovine embryos. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Theriogenology, v. 85, 724-733, 2016. |
DOI: |
org/10.1016/j.theriogenology.2015.10.016 |
Idioma: |
Inglês |
Conteúdo: |
The present study analyzed the changes in gene expression induced by the Cryotop vitrification technique in bovine blastocyst-stage embryos, using Agilent EmbryoGENE microarray slides. Bovine in vitro?produced embryos were vitrified and compared with nonvitrified (control) embryos. After vitrification, embryos were warmed and cultured for an additional 4 hours. Survived embryos were used for microarray analysis and quantitative polymerase chain reaction (qPCR) quantification. Survival rates were higher (P < 0.05) in the control embryos (100%) than in the vitrified embryos (87%). Global gene expression analysis revealed that only 43 out of 21,139 genes exhibited significantly altered expression in the vitrified embryos compared to the control embryos, with a very limited fold change (P < 0.05). A total of 10 genes were assessed by qPCR. Only the FOS-like antigen 1 (FOSL1) gene presented differential expression (P < 0.05) according to both the array and qPCR methods, and it was overexpressed in vitrified embryos. Although, the major consequence of vitrification seems to be the activation of the apoptosis pathway in some cells. Indeed, FOSL1 is part of the activating protein 1 transcription factor complex and is implicated in a variety of cellular processes, including proliferation, differentiation, and apoptosis. Therefore, our results suggest that a limited increase in the rate of apoptosis was the only detectable response of the embryos to vitrification stress. |
Palavras-Chave: |
Cryotop; Embryo; Expressão genética. |
Thesagro: |
Gado. |
Thesaurus NAL: |
cattle; transcriptome; vitrification. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02321naa a2200289 a 4500 001 2033064 005 2023-03-20 008 2016 bl uuuu u00u1 u #d 024 7 $aorg/10.1016/j.theriogenology.2015.10.016$2DOI 100 1 $aLEME, L. de O. 245 $aEffect of vitrification using the Cryotop method on the gene expression profile of in vitro-produced bovine embryos.$h[electronic resource] 260 $c2016 520 $aThe present study analyzed the changes in gene expression induced by the Cryotop vitrification technique in bovine blastocyst-stage embryos, using Agilent EmbryoGENE microarray slides. Bovine in vitro?produced embryos were vitrified and compared with nonvitrified (control) embryos. After vitrification, embryos were warmed and cultured for an additional 4 hours. Survived embryos were used for microarray analysis and quantitative polymerase chain reaction (qPCR) quantification. Survival rates were higher (P < 0.05) in the control embryos (100%) than in the vitrified embryos (87%). Global gene expression analysis revealed that only 43 out of 21,139 genes exhibited significantly altered expression in the vitrified embryos compared to the control embryos, with a very limited fold change (P < 0.05). A total of 10 genes were assessed by qPCR. Only the FOS-like antigen 1 (FOSL1) gene presented differential expression (P < 0.05) according to both the array and qPCR methods, and it was overexpressed in vitrified embryos. Although, the major consequence of vitrification seems to be the activation of the apoptosis pathway in some cells. Indeed, FOSL1 is part of the activating protein 1 transcription factor complex and is implicated in a variety of cellular processes, including proliferation, differentiation, and apoptosis. Therefore, our results suggest that a limited increase in the rate of apoptosis was the only detectable response of the embryos to vitrification stress. 650 $acattle 650 $atranscriptome 650 $avitrification 650 $aGado 653 $aCryotop 653 $aEmbryo 653 $aExpressão genética 700 1 $aDUFORT, I. 700 1 $aSPRICIGO, J. F. W. 700 1 $aBRAGA, T. F. 700 1 $aSIRARD, M. 700 1 $aFRANCO, M. M. 700 1 $aDODE, M. A. N. 773 $tTheriogenology$gv. 85, 724-733, 2016.
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