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 | Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
20/12/2019 |
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Data da última atualização: |
20/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
IARK, D.; BUZZO, A. J. dos R.; GARCIA, J. A. A.; CORREA, V. G.; HELM, C. V.; CORRÊA, R. C. G.; PERALTA, R. A.; MOREIRA, R. de F. P. M.; BRACHT, A.; PERALTA, R. M. |
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Afiliação: |
Daiane Iark, UEM; Ana Júlia dos Reis Buzzo, UEM; Jéssica Amanda Andrade Garcia, UEM; Vanesa Gesser Correa, UEM; CRISTIANE VIEIRA HELM, CNPF; Rúbia Carvalho Gomes Corrêa, UEM; Rosely A. Peralta, UFSC; Regina de Fátima Peralta Muniz Moreira, UFSC; Adelar Bracht, UEM; Rosane Marina Peralta, UEM. |
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Título: |
Enzymatic degradation and detoxification of azo dye Congo red by a new laccase from Oudemansiella canarii. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Bioresource Technology, 289, article 121655, Oct. 2019. 7 p. |
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DOI: |
10.1016/j.biortech.2019.121655 |
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Idioma: |
Inglês |
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Conteúdo: |
A single laccase with molecular weight of 41 kDa was produced by the white-rot fungus Oudemansiella canarii cultured on solid state fermentation using a mixture of sugarcane bagasse-wheat bran as substrate. The enzyme (5 U) was able to decolourize 80% of 50 mg/L Congo red within 24 h at 30 °C and pH 5.5. The relationship between the decolorization rate and dye concentration obeyed Michaelis?Menten kinetics, with KM and Vmax values of 46.180 ± 6.245 µM and 1.840 ± 0.101 µmol/min, respectively. Fourier transform infrared spectroscopy (FTIR) and mass spectrometry allowed to conclude that the laccase acts not only on the dye chromophore group, but also that it cleaves different covalent bonds, causing an effective fragmentation of the molecule. The action of the laccase caused a significant reduction in toxicity, as indicated by the Microtox test. In conclusion, O. canarii laccase could be useful in future biological strategies aiming at degrading azo dyes. |
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Palavras-Chave: |
Biorremediação; Dye degradation; Lacase. |
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Thesaurus Nal: |
Azo dyes; Laccase; White-rot fungi. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 01891naa a2200313 a 4500
001 2117496
005 2019-12-20
008 2019 bl uuuu u00u1 u #d
024 7 $a10.1016/j.biortech.2019.121655$2DOI
100 1 $aIARK, D.
245 $aEnzymatic degradation and detoxification of azo dye Congo red by a new laccase from Oudemansiella canarii.$h[electronic resource]
260 $c2019
520 $aA single laccase with molecular weight of 41 kDa was produced by the white-rot fungus Oudemansiella canarii cultured on solid state fermentation using a mixture of sugarcane bagasse-wheat bran as substrate. The enzyme (5 U) was able to decolourize 80% of 50 mg/L Congo red within 24 h at 30 °C and pH 5.5. The relationship between the decolorization rate and dye concentration obeyed Michaelis?Menten kinetics, with KM and Vmax values of 46.180 ± 6.245 µM and 1.840 ± 0.101 µmol/min, respectively. Fourier transform infrared spectroscopy (FTIR) and mass spectrometry allowed to conclude that the laccase acts not only on the dye chromophore group, but also that it cleaves different covalent bonds, causing an effective fragmentation of the molecule. The action of the laccase caused a significant reduction in toxicity, as indicated by the Microtox test. In conclusion, O. canarii laccase could be useful in future biological strategies aiming at degrading azo dyes.
650 $aAzo dyes
650 $aLaccase
650 $aWhite-rot fungi
653 $aBiorremediação
653 $aDye degradation
653 $aLacase
700 1 $aBUZZO, A. J. dos R.
700 1 $aGARCIA, J. A. A.
700 1 $aCORREA, V. G.
700 1 $aHELM, C. V.
700 1 $aCORRÊA, R. C. G.
700 1 $aPERALTA, R. A.
700 1 $aMOREIRA, R. de F. P. M.
700 1 $aBRACHT, A.
700 1 $aPERALTA, R. M.
773 $tBioresource Technology, 289, article 121655, Oct. 2019. 7 p.
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Registro original: |
Embrapa Florestas (CNPF) |
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Tipo/Formato |
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Registro |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Florestas. Para informações adicionais entre em contato com cnpf.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Florestas. |
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Data corrente: |
12/05/2021 |
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Data da última atualização: |
12/05/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
VIEIRA, T. F.; CORRÊA, R. C. G.; MOREIRA, R. de F. P. M.; PERALTA, R. A.; LIMA, E. A. de; HELM, C. V.; GARCIA, J. A. A.; BRACHT, A.; PERALTA, R. M. |
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Afiliação: |
TATIANE FRANCIELLI VIEIRA, Universidade Estadual de Maringá; RÚBIA CARVALHO GOMES CORRÊA, UniCesumar; REGINA DE FATIMA PERALTA MUNIZ MOREIRA, Universidade Federal de Santa Catarina; ROSELY APARECIDA PERALTA, Universidade Federal de Santa Catarina; EDSON ALVES DE LIMA, CNPF; CRISTIANE VIEIRA HELM, CNPF; JÉSSICA AMANDA ANDRADE GARCIA, Universidade Estadual de Maringá; ADELAR BRACHT, Universidade Estadual de Maringá; ROSANE M. PERALTA, Universidade Estadual de Maringá. |
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Título: |
Valorization of peach palm (Bactris gasipaes Kunth) waste: production of antioxidant xylooligosaccharides. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Waste and Biomass Valorization, 2021. |
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DOI: |
https://doi.org/10.1007/s12649-021-01457-3 |
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Idioma: |
Português |
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Notas: |
Publicado em 6 de maio de 2021. |
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Conteúdo: |
In Brazil, the production and consumption of palm heart, especially from the Bactris gasipaes Kunth, generates a large number of lignocellulosic by-products. This study reports the obtainment of xylooligosaccharides (XOS) from xylans extracted from these residues. Xylans from peach palm waste (inner sheath and peel) were extracted using a mild alkali treatment with recovery yields of 82% and 80%, respectively. XOS were obtained through enzymatic hydrolysis employing a commercial xylanase with yields from xylan inner sheath and xylan peel of 50.1% and 48.8%, respectively. The antioxidant potential of XOS was measured employing five of the most commonly used procedures. In overall terms, the XOS from the xylans of peach palm wastes showed higher antioxidant capacity than the XOS obtained from the commercial xylans. The chemical structures of the XOS were determined by mass spectrometry (ESI?MS). The ESI?MS spectra suggest that XOS with grouped xylose or arabinose units ranging from 2 to 5 (differing by 132 Da) and as sodium adduct ions [M + Na]+ in the range of 100?1000 m/z. These results indicate that peach palm wastes can be explored to XOS production, which could be applied as natural antioxidants in functional food and pharmaceutical preparations. |
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Palavras-Chave: |
Produtos não madereiros; Resíduos de pupunheira; Xilanases; Xilooligossacarídeos. |
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Thesagro: |
Antioxidante. |
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Thesaurus NAL: |
Antioxidants; Xylanases; Xylooligosaccharides. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
LEADER 02304naa a2200337 a 4500
001 2131783
005 2021-05-12
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s12649-021-01457-3$2DOI
100 1 $aVIEIRA, T. F.
245 $aValorization of peach palm (Bactris gasipaes Kunth) waste$bproduction of antioxidant xylooligosaccharides.$h[electronic resource]
260 $c2021
500 $aPublicado em 6 de maio de 2021.
520 $aIn Brazil, the production and consumption of palm heart, especially from the Bactris gasipaes Kunth, generates a large number of lignocellulosic by-products. This study reports the obtainment of xylooligosaccharides (XOS) from xylans extracted from these residues. Xylans from peach palm waste (inner sheath and peel) were extracted using a mild alkali treatment with recovery yields of 82% and 80%, respectively. XOS were obtained through enzymatic hydrolysis employing a commercial xylanase with yields from xylan inner sheath and xylan peel of 50.1% and 48.8%, respectively. The antioxidant potential of XOS was measured employing five of the most commonly used procedures. In overall terms, the XOS from the xylans of peach palm wastes showed higher antioxidant capacity than the XOS obtained from the commercial xylans. The chemical structures of the XOS were determined by mass spectrometry (ESI?MS). The ESI?MS spectra suggest that XOS with grouped xylose or arabinose units ranging from 2 to 5 (differing by 132 Da) and as sodium adduct ions [M + Na]+ in the range of 100?1000 m/z. These results indicate that peach palm wastes can be explored to XOS production, which could be applied as natural antioxidants in functional food and pharmaceutical preparations.
650 $aAntioxidants
650 $aXylanases
650 $aXylooligosaccharides
650 $aAntioxidante
653 $aProdutos não madereiros
653 $aResíduos de pupunheira
653 $aXilanases
653 $aXilooligossacarídeos
700 1 $aCORRÊA, R. C. G.
700 1 $aMOREIRA, R. de F. P. M.
700 1 $aPERALTA, R. A.
700 1 $aLIMA, E. A. de
700 1 $aHELM, C. V.
700 1 $aGARCIA, J. A. A.
700 1 $aBRACHT, A.
700 1 $aPERALTA, R. M.
773 $tWaste and Biomass Valorization, 2021.
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