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Registros recuperados : 3 | |
1. | | ELEOTÉRIO, R. B.; SEPÚLVEDA, R. V.; REIS, E. C. C.; VALENTE, F. L.; BORGES, A P. B. Isolation, expansion and differentiation of mesenchymal stromal cells from rabbits' marrow'. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 5, p. 423-430, maio. 2016. Biblioteca(s): Embrapa Unidades Centrais. |
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2. | | SEPÚLVEDA, R V.; VALENTE, F. L.; REIS, E. C. C.; ARAÚJO, F. R.; ELEOTÉRIO R. B.; QUEIROZ, P. V. S.; BORGES, A. P. B. Bacterial cellulose and bacterial cellulose/polycaprolactone composites as tissue substitutes in rabbits' cornea. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 10, p. 986-992, out. 2016. Biblioteca(s): Embrapa Unidades Centrais. |
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3. | | MURTA, A. R.; ABREU JUNIOR, N. B.; OLIVEIRA, L. S.; REIS, E. C. C.; VALENTE, F. L.; GONÇALVES, G. P.; ELEOTÉRIO, R. B.; BORGES, A. P. B. Perfil epidemiológico e análise microbiológica da infecção de sítio cirúrgico em pacientes humanos e animais de companhia. Pesquisa Veterinária Brasileira v. 35, n.7, p. 652-658, julho 2015. Biblioteca(s): Embrapa Unidades Centrais. |
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Registros recuperados : 3 | |
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Registro Completo
Biblioteca(s): |
Embrapa Unidades Centrais. |
Data corrente: |
31/05/2016 |
Data da última atualização: |
29/12/2017 |
Autoria: |
ELEOTÉRIO, R. B.; SEPÚLVEDA, R. V.; REIS, E. C. C.; VALENTE, F. L.; BORGES, A P. B. |
Afiliação: |
RENATO B. ELEOTÉRIO, UFV; RODRIGO V. SEPULVEDA, UFV; EMILY C. C. REIS, UFV; FABRÍCIO L. VALENTE, UFV; ANDRÉA P. B. BORGES, UFV. |
Título: |
Isolation, expansion and differentiation of mesenchymal stromal cells from rabbits' marrow'. |
Ano de publicação: |
2016 |
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 36, n. 5, p. 423-430, maio. 2016. |
Idioma: |
Inglês |
Conteúdo: |
Tissue engineering has been a fundamental technique in the regenerative medicine field, once it permits to build tri-dimensional tissue constructs associating undifferentiated mesenchymal cells (or mesenchymal stromal cells - MSCs) and scaffolds in vitro. Therefore, many studies have been carried out using these cells from different animal species, and rabbits are often used as animal model for in vivo tissue repair studies. However, most of the information available about MSCs harvesting and characterization is about human and murine cells, which brings some doubts to researchers who desire to work with a rabbit model in tissue repair studies based on MSCs. In this context, this study aimed to add and improve the information available in the scientific literature providing a complete technique for isolation, expansion and differentiation of MSCs from rabbits. Bone marrow mononuclear cells (BMMCs) from humerus and femur of rabbits were obtained and to evaluate their proliferation rate, three different culture media were tested, here referred as DMEM-P, DMEM´S and ?-MEM. The BMMCs were also cultured in osteogenic, chondrogenic and adipogenic induction media to prove their multipotentiality. It was concluded that the techniques suggested in this study can provide a guideline to harvest and isolate MSCs from bone marrow of rabbits in enough amount to allow their expansion and, based on the laboratory experience where the study was developed, it is also suggested a culture media formulation to provide a better cell proliferation rate with multipotentiality preservation. MenosTissue engineering has been a fundamental technique in the regenerative medicine field, once it permits to build tri-dimensional tissue constructs associating undifferentiated mesenchymal cells (or mesenchymal stromal cells - MSCs) and scaffolds in vitro. Therefore, many studies have been carried out using these cells from different animal species, and rabbits are often used as animal model for in vivo tissue repair studies. However, most of the information available about MSCs harvesting and characterization is about human and murine cells, which brings some doubts to researchers who desire to work with a rabbit model in tissue repair studies based on MSCs. In this context, this study aimed to add and improve the information available in the scientific literature providing a complete technique for isolation, expansion and differentiation of MSCs from rabbits. Bone marrow mononuclear cells (BMMCs) from humerus and femur of rabbits were obtained and to evaluate their proliferation rate, three different culture media were tested, here referred as DMEM-P, DMEM´S and ?-MEM. The BMMCs were also cultured in osteogenic, chondrogenic and adipogenic induction media to prove their multipotentiality. It was concluded that the techniques suggested in this study can provide a guideline to harvest and isolate MSCs from bone marrow of rabbits in enough amount to allow their expansion and, based on the laboratory experience where the study was developed, it is also suggested a culture media... Mostrar Tudo |
Palavras-Chave: |
Bone marrow stem cells; Cell therapy; Células tronco mesenquimais; Engenharia de tecido; Medicina regenerativa; Mesenchymal stromal cells; Regenerative medicine; Terapia celular. |
Thesagro: |
Coelho; Medula osséa. |
Thesaurus NAL: |
Rabbits; Tissue engineering. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/143602/1/Isolation-expansion.pdf
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Marc: |
LEADER 02539naa a2200313 a 4500 001 2045981 005 2017-12-29 008 2016 bl uuuu u00u1 u #d 100 1 $aELEOTÉRIO, R. B. 245 $aIsolation, expansion and differentiation of mesenchymal stromal cells from rabbits' marrow'. 260 $c2016 520 $aTissue engineering has been a fundamental technique in the regenerative medicine field, once it permits to build tri-dimensional tissue constructs associating undifferentiated mesenchymal cells (or mesenchymal stromal cells - MSCs) and scaffolds in vitro. Therefore, many studies have been carried out using these cells from different animal species, and rabbits are often used as animal model for in vivo tissue repair studies. However, most of the information available about MSCs harvesting and characterization is about human and murine cells, which brings some doubts to researchers who desire to work with a rabbit model in tissue repair studies based on MSCs. In this context, this study aimed to add and improve the information available in the scientific literature providing a complete technique for isolation, expansion and differentiation of MSCs from rabbits. Bone marrow mononuclear cells (BMMCs) from humerus and femur of rabbits were obtained and to evaluate their proliferation rate, three different culture media were tested, here referred as DMEM-P, DMEM´S and ?-MEM. The BMMCs were also cultured in osteogenic, chondrogenic and adipogenic induction media to prove their multipotentiality. It was concluded that the techniques suggested in this study can provide a guideline to harvest and isolate MSCs from bone marrow of rabbits in enough amount to allow their expansion and, based on the laboratory experience where the study was developed, it is also suggested a culture media formulation to provide a better cell proliferation rate with multipotentiality preservation. 650 $aRabbits 650 $aTissue engineering 650 $aCoelho 650 $aMedula osséa 653 $aBone marrow stem cells 653 $aCell therapy 653 $aCélulas tronco mesenquimais 653 $aEngenharia de tecido 653 $aMedicina regenerativa 653 $aMesenchymal stromal cells 653 $aRegenerative medicine 653 $aTerapia celular 700 1 $aSEPÚLVEDA, R. V. 700 1 $aREIS, E. C. C. 700 1 $aVALENTE, F. L. 700 1 $aBORGES, A P. B. 773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 36, n. 5, p. 423-430, maio. 2016.
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