Data/hora: 23/06/2024 04:04:18

Biblioteca(s): | Embrapa Arroz e Feijão.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | A - 1
Data corrente: | 24/04/2020
Data da última atualização: | 24/04/2020
Autoria: | PEREIRA, W. J.; MELO, A. T. de O.; COELHO, A. S. G.; RODRIGUES, F. A.; MAMIDI, S.; ALENCAR, S. A. de; LANNA, A. C.; VALDISSER, P. A. M. R.; BRONDANI, C.; NASCIMENTO JUNIOR, I. R. do; BORBA, T. C. de O.; VIANELLO, R. P.
Afiliação: | WENDELL JACINTO PEREIRA, UFG; ARTHUR TAVARES DE OLIVEIRA MELO, UFG; ALEXANDRE SIQUEIRA GUEDES COELHO, UFG; FABIANA APARECIDA RODRIGUES; SUJAN MAMIDI, HUDSON ALPHA INSTITUTE FOR BIOTECHNOLOGY, Huntsville-AL; SERGIO AMORIM DE ALENCAR, UNIVERSIDADE CATÓLICA DE BRASÍLIA; ANNA CRISTINA LANNA, CNPAF; PAULA ARIELLE M RIBEIRO VALDISSER, CNPAF; CLAUDIO BRONDANI, CNPAF; IVANILDO RAMALHO DO NASCIMENTO JUNIOR, UNESP, Jaboticabal-SP; TEREZA CRISTINA DE OLIVEIRA BORBA, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF.
Título: | Genome-wide analysis of the transcriptional response to drought stress in root and leaf of common bean.
Ano de publicação: | 2020
Fonte/Imprenta: | Genetics and Molecular Biology, v. 43, n. 1, e20180259, 2020.
ISSN: | 1678-4685
DOI: | https://doi.org/10.1590/1678-4685-gmb-2018-0259
Idioma: | Inglês
Conteúdo: | Genes related to the response to drought stress in leaf and root tissue of drought-susceptible (DS) and tolerant (DT) genotypes were characterized by RNA-Seq. In total, 54,750 transcripts, representative of 28,590 genes, were identified; of these, 1,648 were of high-fidelity (merge of 12 libraries) and described for the first time in the Andean germplasm. From the 1,239 differentially expressed genes (DEGs), 458 were identified in DT, with a predominance of genes in categories of oxidative stress, response to stimulus and kinase activity. Most genes related to oxidation-reduction terms in roots were early triggered in DT (T75) compared to DS (T150) suggestive of a mechanism of tolerance by reducing the damage from ROS. Among the KEGG enriched by DEGs up-regulated in DT leaves, two related to the formation of Sulfur-containing compounds, which are known for their involvement in tolerance to abiotic stresses, were common to all treatments. Through qPCR, 88.64% of the DEGs were validated. A total of 151,283 variants were identified and functional effects estimated for 85,780. The raw data files were submitted to the NCBI database. A transcriptome map revealed new genes and isoforms under drought. These results supports a better understanding of the drought tolerance mechanisms in beans.
Palavras-Chave: | Functional genomics.
Thesagro: | Deficiência Hídrica; Feijão; Folha; Phaseolus Vulgaris; Raiz.
Thesaurus Nal: | Beans; Genomics; Single nucleotide polymorphism; Soil water deficit.
Categoria do assunto: | X Pesquisa, Tecnologia e Engenharia
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/212487/1/CNPAF-2020-GMB.pdf
Registro original: | Embrapa Arroz e Feijão (CNPAF)
Exemplares: | CNPAF - (35776-1) - UPC - AP - DD - 2020 -  - 2020 - DIS