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Registro Completo |
Biblioteca(s): |
Embrapa Meio Ambiente; Embrapa Soja. |
Data corrente: |
28/03/2016 |
Data da última atualização: |
15/04/2016 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
DELAMUTA, J. R. M.; RIBEIRO, R. A.; ORMEÑO-ORRILLO, E.; PARMA, M. M.; MELO, I. S. de; MARTÍNEZ-ROMERO, E.; HUNGRIA, M. |
Afiliação: |
JAKELINE RENATA MARÇON DELAMUTA, UEL; RENAN AUGUSTO RIBEIRO, CNPq; ERNESTO ORMENO-ORRILLO, Universidad Nacional Agraria La Molina; MARCIA MARIA PARMA, CNPMA; ITAMAR SOARES DE MELO, CNPMA; ESPERANZA MARTINEZ-ROMERO, Universidad Nacional Autonoma de Mexico; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
Título: |
Bradyrhizobium tropiciagri sp. nov. and Bradyrhizobium embrapense sp. nov., nitrogen-fixing symbionts of tropical forage legumes. |
Ano de publicação: |
2015 |
Fonte/Imprenta: |
International Journal of Systematic and Evolutionary Microbiology, Reading, v. 65, n. 12, p. 4424-4433, 2015. |
Idioma: |
Português |
Conteúdo: |
Abstract: Biological nitrogen fixation is a key process for agriculture production and environmental sustainability, but there are comparatively few studies of symbionts of tropical pasture legumes, as well as few described Bradyrhizobium species, although it is the predominant 48 genus in the tropics. A detailed polyphasic study was conducted with two Bradyrhizobium strains used in commercial inoculants for tropical pastures in Brazil, CNPSo 1112T isolated from perennial soybean (Neonotonia wightii), and CNPSo 2833T from desmodium (Desmodium heterocarpon). Based on 16S-rRNA phylogeny, both strains were grouped in the B. elkanii superclade, but were not clearly clustered with any known species. MLSA of three (glnII, gyrB and recA) and five (+ atpD and dnaK) housekeeping genes confirmed that the strains are positioned in two distinct clades. Comparison with ITS sequences of described Bradyrhizobium species showed similarity lower than 93.1%, and differences were confirmed by BOX-PCR analysis. Nucleotide identity of three housekeeping genes with described species ranged from 88.1% to 96.2%. ANI of genome sequences showed values below the threshold of Bradyrhizobium species (? 90.6%) and also between the two strains (91.2%). The analysis of nifH and nodC genes positioned the two strains in a distinct clade from other Bradyrhizobium species. Morpho-physiological, genotypic and genomic data supported the description of two novel clades in the genus Bradyrhizobium, B. tropiciagri sp. nov. (type strain CNPSo 1112T =SMS 303T =BR 1009T =SEMIA 6148T =LMG 28867T) and B. embrapense sp. nov. (type strain CNPSo 2833T =CIAT 2372T =BR 2212T =SEMIA 6208T =U674T). MenosAbstract: Biological nitrogen fixation is a key process for agriculture production and environmental sustainability, but there are comparatively few studies of symbionts of tropical pasture legumes, as well as few described Bradyrhizobium species, although it is the predominant 48 genus in the tropics. A detailed polyphasic study was conducted with two Bradyrhizobium strains used in commercial inoculants for tropical pastures in Brazil, CNPSo 1112T isolated from perennial soybean (Neonotonia wightii), and CNPSo 2833T from desmodium (Desmodium heterocarpon). Based on 16S-rRNA phylogeny, both strains were grouped in the B. elkanii superclade, but were not clearly clustered with any known species. MLSA of three (glnII, gyrB and recA) and five (+ atpD and dnaK) housekeeping genes confirmed that the strains are positioned in two distinct clades. Comparison with ITS sequences of described Bradyrhizobium species showed similarity lower than 93.1%, and differences were confirmed by BOX-PCR analysis. Nucleotide identity of three housekeeping genes with described species ranged from 88.1% to 96.2%. ANI of genome sequences showed values below the threshold of Bradyrhizobium species (? 90.6%) and also between the two strains (91.2%). The analysis of nifH and nodC genes positioned the two strains in a distinct clade from other Bradyrhizobium species. Morpho-physiological, genotypic and genomic data supported the description of two novel clades in the genus Bradyrhizobium, B. tropiciagri ... Mostrar Tudo |
Palavras-Chave: |
ANI; Biological nitrogen fixation; Inoculant; MLSA. |
Thesaurus Nal: |
Bradyrhizobium; nitrogen-fixing bacteria; nodulation. |
Categoria do assunto: |
-- S Ciências Biológicas |
Marc: |
LEADER 02567naa a2200277 a 4500 001 2041843 005 2016-04-15 008 2015 bl uuuu u00u1 u #d 100 1 $aDELAMUTA, J. R. M. 245 $aBradyrhizobium tropiciagri sp. nov. and Bradyrhizobium embrapense sp. nov., nitrogen-fixing symbionts of tropical forage legumes.$h[electronic resource] 260 $c2015 520 $aAbstract: Biological nitrogen fixation is a key process for agriculture production and environmental sustainability, but there are comparatively few studies of symbionts of tropical pasture legumes, as well as few described Bradyrhizobium species, although it is the predominant 48 genus in the tropics. A detailed polyphasic study was conducted with two Bradyrhizobium strains used in commercial inoculants for tropical pastures in Brazil, CNPSo 1112T isolated from perennial soybean (Neonotonia wightii), and CNPSo 2833T from desmodium (Desmodium heterocarpon). Based on 16S-rRNA phylogeny, both strains were grouped in the B. elkanii superclade, but were not clearly clustered with any known species. MLSA of three (glnII, gyrB and recA) and five (+ atpD and dnaK) housekeeping genes confirmed that the strains are positioned in two distinct clades. Comparison with ITS sequences of described Bradyrhizobium species showed similarity lower than 93.1%, and differences were confirmed by BOX-PCR analysis. Nucleotide identity of three housekeeping genes with described species ranged from 88.1% to 96.2%. ANI of genome sequences showed values below the threshold of Bradyrhizobium species (? 90.6%) and also between the two strains (91.2%). The analysis of nifH and nodC genes positioned the two strains in a distinct clade from other Bradyrhizobium species. Morpho-physiological, genotypic and genomic data supported the description of two novel clades in the genus Bradyrhizobium, B. tropiciagri sp. nov. (type strain CNPSo 1112T =SMS 303T =BR 1009T =SEMIA 6148T =LMG 28867T) and B. embrapense sp. nov. (type strain CNPSo 2833T =CIAT 2372T =BR 2212T =SEMIA 6208T =U674T). 650 $aBradyrhizobium 650 $anitrogen-fixing bacteria 650 $anodulation 653 $aANI 653 $aBiological nitrogen fixation 653 $aInoculant 653 $aMLSA 700 1 $aRIBEIRO, R. A. 700 1 $aORMEÑO-ORRILLO, E. 700 1 $aPARMA, M. M. 700 1 $aMELO, I. S. de 700 1 $aMARTÍNEZ-ROMERO, E. 700 1 $aHUNGRIA, M. 773 $tInternational Journal of Systematic and Evolutionary Microbiology, Reading$gv. 65, n. 12, p. 4424-4433, 2015.
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Embrapa Meio Ambiente (CNPMA) |
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Registro Completo
Biblioteca(s): |
Embrapa Gado de Corte; Embrapa Pantanal. |
Data corrente: |
03/02/1999 |
Data da última atualização: |
15/03/1999 |
Autoria: |
SILVA, J. dos S. V. da; ABDON, M. de M.; BOOCK, A.; SILVA, M. P. da. |
Afiliação: |
EMBRAPA. Centro de Pesquisa Agropecuaria do Pantanal (Corumba, MS). |
Título: |
Fitofisionomias dominantes em parte das sub-regioes do Nabileque e Miranda, sul do Pantanal. |
Ano de publicação: |
1998 |
Fonte/Imprenta: |
Pesquisa Agropecuaria Brasileira, Brasilia, v.33, n. especial, p.1713-1719, out. 1998. |
Idioma: |
Português |
Notas: |
CNPG. CPAP. |
Conteúdo: |
Objetiva-se definir uma metodologia de discriminacao de fitofisionomias em ambiente alagavel, por meio de produtos de sensoriamento remoto e trabalhos de campo. A area avaliada localiza-se no sul do Pantanal. O estudo consistiu na interpretacao visual de imagens TM(Thematic Mapper) analogicas do satelite Landsat, correspondente a epoca seca (setembro/89), na escala de 1:100.000, obtidas nas bandas individuais 4 e 5 em branco e preto e em composicao colorida 3 (azul), 4 (verde) e 5 (vermelho). Definiram-se, nas imagens, areas amostrais com diferentes padroes de cor, textura e forma associadas a diferentes fitofionomias, sendo estas verificadas em campo. Utilizaram-se, tambem, fotos aereas pancromaticas na escala de 1:20.000, do ano de 1974, para avaliar areas em que os limites das fitofisionomias nao se apresentaram nitidos. A metodologia utilizada permitiu identifica a cobertura vegetal diferenciada estruturalmente, correspondente aos estratos arboreo, arbusivo e herbaceo, subdivididos em 14 classes de mapeamento, associadas as fitofisionomias dominantes distintas floristicamente e conhecidas regionalmente por paratudal (Tabeluia aurea), carandazal (Copernicia alba), mata ciliar, mata semidecidual, caapao de mata, mata mista de carandazal, paratudal e semidecidual, canjiqueiral (Byrsonima orbignyna), espinheiral, espinheiral inundado, estadio seral da mata ciliar, brejo, campo de gramineas e arbustos, e campo inundado. |
Palavras-Chave: |
Areas alagaveis; Brasil; Flooded areas; Flooded land; Miranda; Nabilique; Remoto sensing. |
Thesagro: |
Sensoriamento Remoto; Vegetação. |
Thesaurus NAL: |
Brazil; Pantanal; remote sensing; vegetation. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02339naa a2200325 a 4500 001 1320823 005 1999-03-15 008 1998 bl uuuu u00u1 u #d 100 1 $aSILVA, J. dos S. V. da 245 $aFitofisionomias dominantes em parte das sub-regioes do Nabileque e Miranda, sul do Pantanal. 260 $c1998 500 $aCNPG. CPAP. 520 $aObjetiva-se definir uma metodologia de discriminacao de fitofisionomias em ambiente alagavel, por meio de produtos de sensoriamento remoto e trabalhos de campo. A area avaliada localiza-se no sul do Pantanal. O estudo consistiu na interpretacao visual de imagens TM(Thematic Mapper) analogicas do satelite Landsat, correspondente a epoca seca (setembro/89), na escala de 1:100.000, obtidas nas bandas individuais 4 e 5 em branco e preto e em composicao colorida 3 (azul), 4 (verde) e 5 (vermelho). Definiram-se, nas imagens, areas amostrais com diferentes padroes de cor, textura e forma associadas a diferentes fitofionomias, sendo estas verificadas em campo. Utilizaram-se, tambem, fotos aereas pancromaticas na escala de 1:20.000, do ano de 1974, para avaliar areas em que os limites das fitofisionomias nao se apresentaram nitidos. A metodologia utilizada permitiu identifica a cobertura vegetal diferenciada estruturalmente, correspondente aos estratos arboreo, arbusivo e herbaceo, subdivididos em 14 classes de mapeamento, associadas as fitofisionomias dominantes distintas floristicamente e conhecidas regionalmente por paratudal (Tabeluia aurea), carandazal (Copernicia alba), mata ciliar, mata semidecidual, caapao de mata, mata mista de carandazal, paratudal e semidecidual, canjiqueiral (Byrsonima orbignyna), espinheiral, espinheiral inundado, estadio seral da mata ciliar, brejo, campo de gramineas e arbustos, e campo inundado. 650 $aBrazil 650 $aPantanal 650 $aremote sensing 650 $avegetation 650 $aSensoriamento Remoto 650 $aVegetação 653 $aAreas alagaveis 653 $aBrasil 653 $aFlooded areas 653 $aFlooded land 653 $aMiranda 653 $aNabilique 653 $aRemoto sensing 700 1 $aABDON, M. de M. 700 1 $aBOOCK, A. 700 1 $aSILVA, M. P. da 773 $tPesquisa Agropecuaria Brasileira, Brasilia$gv.33, n. especial, p.1713-1719, out. 1998.
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