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| Registros recuperados : 54 | |
| 41. |  | COSTA, R. C.; ISHIDA, A. K. N.; BONFIM, K.; CARVALHO, E. A.; OLIVEIRA, L. C.; DAMASCENO FILHO, A. S.; RESENDE, M. L. V. Acibenzolar-s-metil, oxicloreto de cobre e fertilizantes foliares no controle da mancha bacteriana do maracujazeiro. Tropical Plant Pathology, v. 36, p. 0797, ago. 2011. Suplemento. Edição dos resumos do 44º Congresso Brasileiro de Fitopatologia, 2011, Bento Gonçalves. Resumo 1172.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 42. | | MONNERAT, R. G.; SILVA, S. F. da; BONFIM, K.; SILVA-WERNECK, J. O. da; NOBRE, S. D.; DIAS, J. M. C. de S. banco de germoplasma de Bacillus spp. entomopatogenicos. In: SIMPOSIO DE RECURSOS GENETICOS PARA AMERICA LATINA E CARIBE, 2., 1999, Brasilia, DF. Anais... Brasilia: Embrapa Recursos Geneticos e Biotecnologia, 1999. CD-ROM.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 43. | | ALMEIDA, L. M.; CIPRIANO, T. M.; BONFIM, K.; NOGUEIRA, E. O. P. e L.; RIBEIRO, S. da G.; ARAGAO, F. J. L. Begomovirus resistance in transgenic tomatoes. In: CONGRESSO BRASILEIRO DE BIOTECNOLOGIA, 3., 2010, Fortaleza. Programa e resumos. Brasília, DF: SBBiotec, 2010. p. 303.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 44. | | ISHIDA, A. K. N.; NORONHA, A. C. da S.; MENDONÇA, C. L. G.; BONFIM, K.; OLIVEIRA, L. C. de; PROTAZIO, D. C. Incidência da mancha aureolada em pomares cítricos no estado do Pará. In: CONFERÊNCIA NACIONAL DE DEFESA AGROPECUÁRIA, 4., 2013, Belém, PA. Defesa agropecuária e sustentabilidade. Belém, PA: SBDA, 2014. p. 187.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 45. | | SPRICIGO, P. C.; PILON, L.; TRENTO, J. P.; MOURA, M. R. de; BONFIM, K. S.; FOSCHINI, M. M.; MATTOSO, L. H. C.; FERREIRA, M. D. Nano-chitosan as an antimicrobial agent in preservative solutions for cut flowers. Journal of Chemical Technology and Biotechnology, v. 96, n. 8, p. 2168-2175, Aug. 2021. 8 p. Na publicação: Milene C Mitsuyuki.| Biblioteca(s): Embrapa Hortaliças; Embrapa Instrumentação. |
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| 46. | | ISHIDA, A. K. N.; COSTA, R. C. da; MIRANDA, V. S.; BONFIM, K.; OLIVEIRA, L. C. de; RIBEIRO JÚNIOR, P. M.; RESENDE, M. L. V. de. Indução de resistência no controle da mancha bacteriana do maracujazeiro. In: CONGRESSO BRASILEIRO DE DEFENSIVOS AGRÍCOLAS NATURAIS, 6., 2013, João Pessoa. Anais... João Pessoa: UEPB: Embrapa, 2013. p. 134.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 48. | | ARAÚJO, F. C. B. das; CUNHA, E. F. M.; RODRIGUES, S. de M.; BONFIM, K.; TAVARES, E. J. M.; MOURA, M. F.; SILVA, G. F. da. Identification and validation of SNPs in the phytoene synthase 2 (psy2) gene associated with yellow color of the root in cassava (Manihot esculenta Crantz) accessions of the Brazilian Amazon. Genetic Resources and Crop Evolution, v. 68, p. 1809-1824, 2021.| Biblioteca(s): Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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| 49. | | DAMASCENO FILHO, A. S.; ISHIDA, A. K. N.; NASCIMENTO, W. M. O.; CARVALHO, J. E. U.; SOUZA, C. A. S.; OLIVEIRA, L. C.; BONFIM, K. Reação de plantas de maracujazeiro amarelo a Xanthomonas axonopodis pv. passiflorae. Tropical Plant Pathology, Lavras, MG, v. 35, p. S214, Aug. 2010. Suplemento. Edição dos resumos do XLIII Congresso Brasileiro de Fitopatologia, Cuiabá, ago. 2010.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 50. | | RIBEIRO, S. da G.; CIPRIANO, T.; NOGUEIRA, E. O. P. e L.; BONFIM, K.; TINOCO, M. L.; ARAGAO, F. J. L. RNAI-mediated resistance to bipartite begomoviruses in genetically modified tomatoes. In: INTERNATIONAL CONGRESS OF PLANT MOLECULAR BIOLOGY, 9., 2009, St. Louis, Missouri. Proceedings... [S.l.]: The International Society for Plant Molecular Biology, 2009.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 51. | | PIZETTA, C. S. R.; RIBEIRO, W. R.; FERREIRA, A. L.; MOURA, M. da C.; BONFIM, K.; PINHEIRO, P. V.; CABRAL, G. B.; ARAGÃO, F. J. L. RNA interference-mediated tolerance to whitefly (Bemisia tabaci) in genetically engineered tomato. Plant Cell, Tissue and Organ Culture, v. 148, p. 281-291, 2022. Na publicação: Kenny Bonfim.| Biblioteca(s): Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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| 52. | | ALMEIDA, L. M.; CIPRIANO, T. M.; BONFIM, K.; NOGUEIRA, E. O. P. e L.; RIBEIRO, S. da G.; ARAGAO, F. J. L. RNAI-resistance to bipartite Begomoviruses in genetically modified Tomatoes. In: INTERNATIONAL GEMINIVIRUS SYMPOSIUM, 6.; INTERNATIONAL ssDNA COMPARATIVE VIROLOGY WORKSHOP, 4., 2010, Guanajuato, Mexico. [Programme & abstracts]. Guanajuato: Cinvestav: Conacyt, 2010.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 53. | | ALMEIDA, L. M.; CIPRIANO, T. M.; BONFIM, K.; NOGUEIRA, E. O. P. e L.; RIBEIRO, S. da G.; ARAGAO, F. J. L. Resistência mediada por RNAi à begomovírus bipartidos em tomates geneticamente modificados. In: ENCONTRO DO TALENTO ESTUDANTIL DA EMBRAPA RECURSOS GENÉTICOS E BIOTECNOLOGIA, 15., 2010, Brasília, DF. Anais: resumos dos trabalhos. Brasília, DF: Embrapa Recursos Genéticos e Biotecnologia, 2010. Resumo 032.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 54. | | VIANNA, G. R.; IRALA, L. H.; SANTOS, C. R.; RAMA SWAMI, N.; BONFIM, K.; SOUZA JÚNIOR, M. T.; ARAGÃO, F. J. L.; RECH, E. L. Transformação genética de tabaco visando obter plantas mais tolerantes a estresses abióticos. In: CONGRESSO BRASILEIRO DE GENÉTICA, 53., 2007, Águas de Lindóia. Resumos... Águas de Lindóia: Sociedade Brasileira de Genética, 2007.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 54 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Oriental. Para informações adicionais entre em contato com cpatu.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
28/01/2021 |
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Data da última atualização: |
10/06/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
ARAÚJO, F. C. B. das; CUNHA, E. F. M.; RODRIGUES, S. de M.; BONFIM, K.; TAVARES, E. J. M.; MOURA, M. F.; SILVA, G. F. da. |
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Afiliação: |
Francisca Chagas Bezerra das Araújo, UFRA; ELISA FERREIRA MOURA CUNHA, CPATU; SIMONE DE MIRANDA RODRIGUES, CPATU; KENNY BONFIM DE ARRUDA CARVALHO, Cenargen; ERALDO JOSE MADUREIRA TAVARES, CPATSA; Mônika Fecury Moura, CPATU; GILVAN FERREIRA DA SILVA, CPAA. |
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Título: |
Identification and validation of SNPs in the phytoene synthase 2 (psy2) gene associated with yellow color of the root in cassava (Manihot esculenta Crantz) accessions of the Brazilian Amazon. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Genetic Resources and Crop Evolution, v. 68, p. 1809-1824, 2021. |
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DOI: |
https://doi.org/10.1007/s10722-020-01097-x |
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Idioma: |
Inglês |
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Conteúdo: |
The biofortification of cassava (Manihot esculenta Crantz) roots is one of the actual goals of genetic breeding, with the development of roots richer in carotenoids. In order to evaluate the natural genetic variation of cassava, this study analyzed 78 accessions from the Brazilian Amazon to verify the presence of single nucleotide polymorphism (SNP) molecular markers in the phytoene synthase 2 (psy2) gene associated with root color. From the sequencing of 294 bp containing part of the intron 2 and the exon 3 of the psy2 gene, three SNPs located in the coding region and the existence of eight haplotypes were identified. The c.1434G > C (SNP1) and c.1485C > A (SNP3) variations were found in yellow and cream root accessions and nucleotide polymorphism resulted in amino acid substitution. SNP2 (c.1462T > C) was identified in accessions with white roots and its allelic variation did not result in amino acid substitution. Four haplotypes that presented SNP3 were widely distributed in the states of Pará, Amapá, Rondônia and Amazonas. All accessions of yellow or cream root that presented the SNP3 were in heterozygosity, confirming the dominance of the SNP, except for one accession, that could be used in genetic breeding. The effect of mutations on the structure and function of PSY2 was in silico evaluated by molecular dynamics and it was indicated that the aminoacid substitutions affected the active site of the protein depending on the distance. The results were compatible with the phenotype detected experimentally. MenosThe biofortification of cassava (Manihot esculenta Crantz) roots is one of the actual goals of genetic breeding, with the development of roots richer in carotenoids. In order to evaluate the natural genetic variation of cassava, this study analyzed 78 accessions from the Brazilian Amazon to verify the presence of single nucleotide polymorphism (SNP) molecular markers in the phytoene synthase 2 (psy2) gene associated with root color. From the sequencing of 294 bp containing part of the intron 2 and the exon 3 of the psy2 gene, three SNPs located in the coding region and the existence of eight haplotypes were identified. The c.1434G > C (SNP1) and c.1485C > A (SNP3) variations were found in yellow and cream root accessions and nucleotide polymorphism resulted in amino acid substitution. SNP2 (c.1462T > C) was identified in accessions with white roots and its allelic variation did not result in amino acid substitution. Four haplotypes that presented SNP3 were widely distributed in the states of Pará, Amapá, Rondônia and Amazonas. All accessions of yellow or cream root that presented the SNP3 were in heterozygosity, confirming the dominance of the SNP, except for one accession, that could be used in genetic breeding. The effect of mutations on the structure and function of PSY2 was in silico evaluated by molecular dynamics and it was indicated that the aminoacid substitutions affected the active site of the protein depending on the distance. T... Mostrar Tudo |
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Palavras-Chave: |
Biofortificação; Raíz rica em carotenoide. |
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Thesagro: |
Carotenóide; Mandioca; Manihot Esculenta; Polimorfismo; Proteína; Raiz Alimentícia; Variação Genética. |
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Thesaurus NAL: |
Carotenoids; Cassava; Haplotypes; Polymorphism. |
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Categoria do assunto: |
--
G Melhoramento Genético |
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Marc: |
LEADER 02740naa a2200361 a 4500
001 2129648
005 2021-06-10
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s10722-020-01097-x$2DOI
100 1 $aARAÚJO, F. C. B. das
245 $aIdentification and validation of SNPs in the phytoene synthase 2 (psy2) gene associated with yellow color of the root in cassava (Manihot esculenta Crantz) accessions of the Brazilian Amazon.$h[electronic resource]
260 $c2021
520 $aThe biofortification of cassava (Manihot esculenta Crantz) roots is one of the actual goals of genetic breeding, with the development of roots richer in carotenoids. In order to evaluate the natural genetic variation of cassava, this study analyzed 78 accessions from the Brazilian Amazon to verify the presence of single nucleotide polymorphism (SNP) molecular markers in the phytoene synthase 2 (psy2) gene associated with root color. From the sequencing of 294 bp containing part of the intron 2 and the exon 3 of the psy2 gene, three SNPs located in the coding region and the existence of eight haplotypes were identified. The c.1434G > C (SNP1) and c.1485C > A (SNP3) variations were found in yellow and cream root accessions and nucleotide polymorphism resulted in amino acid substitution. SNP2 (c.1462T > C) was identified in accessions with white roots and its allelic variation did not result in amino acid substitution. Four haplotypes that presented SNP3 were widely distributed in the states of Pará, Amapá, Rondônia and Amazonas. All accessions of yellow or cream root that presented the SNP3 were in heterozygosity, confirming the dominance of the SNP, except for one accession, that could be used in genetic breeding. The effect of mutations on the structure and function of PSY2 was in silico evaluated by molecular dynamics and it was indicated that the aminoacid substitutions affected the active site of the protein depending on the distance. The results were compatible with the phenotype detected experimentally.
650 $aCarotenoids
650 $aCassava
650 $aHaplotypes
650 $aPolymorphism
650 $aCarotenóide
650 $aMandioca
650 $aManihot Esculenta
650 $aPolimorfismo
650 $aProteína
650 $aRaiz Alimentícia
650 $aVariação Genética
653 $aBiofortificação
653 $aRaíz rica em carotenoide
700 1 $aCUNHA, E. F. M.
700 1 $aRODRIGUES, S. de M.
700 1 $aBONFIM, K.
700 1 $aTAVARES, E. J. M.
700 1 $aMOURA, M. F.
700 1 $aSILVA, G. F. da
773 $tGenetic Resources and Crop Evolution$gv. 68, p. 1809-1824, 2021.
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