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Registro Completo |
Biblioteca(s): |
Embrapa Hortaliças. |
Data corrente: |
27/09/2012 |
Data da última atualização: |
24/05/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
ARAÚJO, E. R.; COSTA, J. R.; FERREIRA, M. A. S. V.; QUEZADO-DUVAL, A. M. |
Afiliação: |
ALICE MARIA QUEZADO DUVAL, CNPH. |
Título: |
Simultaneous detection and identification of the Xanthomonas species complex associated with tomato bacterial spot using species-specifics primers and multiples PCR. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
Journal of Applied Microbiology, Oxford, Sept. 2012. |
ISSN: |
1364-5072 |
DOI: |
10.1111/j.1365-2672.05431.x |
Idioma: |
Inglês |
Conteúdo: |
To establish protocols for the simultaneous detection and identification of Xanthomonas species causing tomato bacterial spot. Methods and Results: We verified the specificity and sensitivity of the previously reported sets of primers designed for strains of the four species of Brazilian tomato bacterial spot xanthomonads, consisting of 30 of Xanthomonas euvesicatoria, 30 of X. vesicatoria,50ofX. perforans and 50 of X. gardneri. Furthermore, we tested a multiplex PCR protocol for the purpose of concurrent species identification. |
Palavras-Chave: |
Detecção; PCR. |
Thesagro: |
Bactéria; Doença; Identificação. |
Thesaurus Nal: |
Xanthomonas. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/177608/1/Ara-jo-et-al-2012-Journal-of-Applied-Microbiology.pdf
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Marc: |
LEADER 01335naa a2200253 a 4500 001 1934722 005 2018-05-24 008 2012 bl uuuu u00u1 u #d 022 $a1364-5072 024 7 $a10.1111/j.1365-2672.05431.x$2DOI 100 1 $aARAÚJO, E. R. 245 $aSimultaneous detection and identification of the Xanthomonas species complex associated with tomato bacterial spot using species-specifics primers and multiples PCR.$h[electronic resource] 260 $c2012 520 $aTo establish protocols for the simultaneous detection and identification of Xanthomonas species causing tomato bacterial spot. Methods and Results: We verified the specificity and sensitivity of the previously reported sets of primers designed for strains of the four species of Brazilian tomato bacterial spot xanthomonads, consisting of 30 of Xanthomonas euvesicatoria, 30 of X. vesicatoria,50ofX. perforans and 50 of X. gardneri. Furthermore, we tested a multiplex PCR protocol for the purpose of concurrent species identification. 650 $aXanthomonas 650 $aBactéria 650 $aDoença 650 $aIdentificação 653 $aDetecção 653 $aPCR 700 1 $aCOSTA, J. R. 700 1 $aFERREIRA, M. A. S. V. 700 1 $aQUEZADO-DUVAL, A. M. 773 $tJournal of Applied Microbiology, Oxford, Sept. 2012.
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Embrapa Hortaliças (CNPH) |
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Registro Completo
Biblioteca(s): |
Embrapa Hortaliças. |
Data corrente: |
22/12/1994 |
Data da última atualização: |
23/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
Internacional - A |
Autoria: |
BOITEUX, L. S.; AVILA, A. C. de. |
Afiliação: |
LEONARDO SILVA BOITEUX, CNPH; ANTONIO CARLOS DE AVILA, CNPH. |
Título: |
Inheritance of a resistance specific to tomato spotted wilt tospovirus in Capsicum chinense 'PI 159236'. |
Ano de publicação: |
1994 |
Fonte/Imprenta: |
Euphytica, v. 75, p. 139-142, 1994. |
Idioma: |
Inglês |
Conteúdo: |
Inheritance studies were conducted to determine the genetic basis of resistance in pepper against one Tospovirus isolate classifie as tomato spotted wilt virus (TSWV). F1, backcrosses and F2 populations were develop using the resistant parent Capsicum chinense 'PI 159236' (CNPH 679) and the susceptible parent C. annuum 'Magda' (CNPH 192). Segregation ratios strongly indicated that the resistant response (a localization, hypersensitive-like reaction) to TSWV fits a single-dominant gene model. Under our experimental conditions, the penetrance of this gene was very high. This gene (tentatively named Tsw) is highly effective only against TSWV isolates. The resistance governed by the Tsw gene was not effective against isolates belonging to tomato chlorotic spotvirus (TCSV) and groundnut ring spot virus (GRSV), two other previously described Tospovirus species. |
Palavras-Chave: |
Capsium annuum; Disease; Heranca; Inheritance; Resistance; Sweet pepper; TSWV. |
Thesagro: |
Capsicum Chinense; Cerrado; Doença; Pimentão; Resistência; Vírus. |
Thesaurus NAL: |
Tospovirus. |
Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
Marc: |
LEADER 01657naa a2200301 a 4500 001 1752347 005 2024-02-23 008 1994 bl uuuu u00u1 u #d 100 1 $aBOITEUX, L. S. 245 $aInheritance of a resistance specific to tomato spotted wilt tospovirus in Capsicum chinense 'PI 159236'.$h[electronic resource] 260 $c1994 520 $aInheritance studies were conducted to determine the genetic basis of resistance in pepper against one Tospovirus isolate classifie as tomato spotted wilt virus (TSWV). F1, backcrosses and F2 populations were develop using the resistant parent Capsicum chinense 'PI 159236' (CNPH 679) and the susceptible parent C. annuum 'Magda' (CNPH 192). Segregation ratios strongly indicated that the resistant response (a localization, hypersensitive-like reaction) to TSWV fits a single-dominant gene model. Under our experimental conditions, the penetrance of this gene was very high. This gene (tentatively named Tsw) is highly effective only against TSWV isolates. The resistance governed by the Tsw gene was not effective against isolates belonging to tomato chlorotic spotvirus (TCSV) and groundnut ring spot virus (GRSV), two other previously described Tospovirus species. 650 $aTospovirus 650 $aCapsicum Chinense 650 $aCerrado 650 $aDoença 650 $aPimentão 650 $aResistência 650 $aVírus 653 $aCapsium annuum 653 $aDisease 653 $aHeranca 653 $aInheritance 653 $aResistance 653 $aSweet pepper 653 $aTSWV 700 1 $aAVILA, A. C. de 773 $tEuphytica$gv. 75, p. 139-142, 1994.
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