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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
03/10/2011 |
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Data da última atualização: |
02/05/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARRACCINI, P.; FREIRE, L. P.; ALVES, G. S. C.; VIEIRA, N. G.; VINECKY, F.; ELBELT, S.; RAMOS, H. J; MONTAGNON, C.; VIEIRA, L. G. E.; LEROY, T.; POT, D.; SILVA, V. A.; RODRIGUES, G. C.; ANDRADE, A. C. |
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Afiliação: |
CIRAD; LUCIANA PEREIRA FREIRE; GABRIEL SERGIO COSTA ALVES; NATALIA GOMES VIEIRA; FELIPE VINECKY; SÔNIA ELBERT, CENARGEN; INSTITUTO AGRONOMICO DO PARANÁ; CIRAD; INSTITUTO AGRONOMICO PARANÁ; AGAP; AGAP; EPAMIG; GUSTAVO COSTA RODRIGUES, CNPTIA; ALAN CARVALHO ANDRADE, CENARGEN. |
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Título: |
RBCS1 expression in coffee: coffea orthologs, coffea arabica homeologs, and expression variability between genotypes and under drought stress |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
BMC Plant Biology, v. 11, n. 85, 2011. |
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Idioma: |
Inglês |
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Conteúdo: |
Background: In higher plants, the inhibition of photosynthetic capacity under drought is attributable to stomatal and non-stomatal (i.e., photochemical and biochemical) effects. In particular, a disruption of photosynthetic metabolism and Rubisco regulation can be observed. Several studies reported reduced expression of the RBCS genes, which encode the Rubisco small subunit, under water stress. Results: Expression of the RBCS1 gene was analysed in the allopolyploid context of C. arabica, which originates from a natural cross between the C. canephora and C. eugenioides species. Our study revealed the existence of two homeologous RBCS1 genes in C. arabica: one carried by the C. canephora sub-genome (called CaCc) and the other carried by the C. eugenioides sub-genome (called CaCe). Using specific primer pairs for each homeolog, expression studies revealed that CaCe was expressed in C. eugenioides and C. arabica but was undetectable in C. canephora. On the other hand, CaCc was expressed in C. canephora but almost completely silenced in non-introgressed ("pure?) genotypes of C. arabica. However, enhanced CaCc expression was observed in most C. arabica cultivars with introgressed C. canephora genome. In addition, total RBCS1 expression was higher for C. arabica cultivars that had recently introgressed C. canephora genome than for ?pure? cultivars. For both species, water stress led to an important decrease in the abundance of RBCS1 transcripts. This was observed for plants grown in either greenhouse or field conditions under severe or moderate drought. However, this reduction of RBCS1 gene expression was not accompanied by a decrease in the corresponding protein in the leaves of C. canephora subjected to water withdrawal. In that case, the amount of RBCS1 was even higher under drought than under unstressed (irrigated) conditions, which suggests great stability of RBCS1 under adverse water conditions. On the other hand, for C. arabica, high nocturnal expression of RBCS1 could also explain the accumulation of the RBCS1 protein under water stress. Altogether, the results presented here suggest that the content of RBCS was not responsible for the loss of photosynthetic capacity that is commonly observed in water-stressed coffee plants. Conclusion: We showed that the CaCe homeolog was expressed in C. eugenioides and non-introgressed ("pure?) genotypes of C. arabica but that it was undetectable in C. canephora. On the other hand, the CaCc homeolog was expressed in C. canephora but highly repressed in C. arabica. Expression of the CaCc homeolog was enhanced in C. arabica cultivars that experienced recent introgression with C. canephora. For both C. canephora and C. arabica species, total RBCS1 gene expression was highly reduced with WS. Unexpectedly, the accumulation of RBCS1 protein was observed in the leaves of C. canephora under WS, possibly coming from nocturnal RBCS1 expression. These results suggest that the increase in the amount of RBCS1 protein could contribute to the antioxidative function of photorespiration in water-stressed coffee plants. MenosBackground: In higher plants, the inhibition of photosynthetic capacity under drought is attributable to stomatal and non-stomatal (i.e., photochemical and biochemical) effects. In particular, a disruption of photosynthetic metabolism and Rubisco regulation can be observed. Several studies reported reduced expression of the RBCS genes, which encode the Rubisco small subunit, under water stress. Results: Expression of the RBCS1 gene was analysed in the allopolyploid context of C. arabica, which originates from a natural cross between the C. canephora and C. eugenioides species. Our study revealed the existence of two homeologous RBCS1 genes in C. arabica: one carried by the C. canephora sub-genome (called CaCc) and the other carried by the C. eugenioides sub-genome (called CaCe). Using specific primer pairs for each homeolog, expression studies revealed that CaCe was expressed in C. eugenioides and C. arabica but was undetectable in C. canephora. On the other hand, CaCc was expressed in C. canephora but almost completely silenced in non-introgressed ("pure?) genotypes of C. arabica. However, enhanced CaCc expression was observed in most C. arabica cultivars with introgressed C. canephora genome. In addition, total RBCS1 expression was higher for C. arabica cultivars that had recently introgressed C. canephora genome than for ?pure? cultivars. For both species, water stress led to an important decrease in the abundance of RBCS1 transcripts. This was observed for plants grown i... Mostrar Tudo |
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Palavras-Chave: |
Expressão gênica; Sequência genética. |
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Thesagro: |
Café; Coffea Arábica; Coffea Canephora; Genética Molecular. |
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Thesaurus Nal: |
Gene expression; Molecular genetics. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/46090/1/1471-2229-11-85-1.pdf
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Marc: |
LEADER 04164naa a2200373 a 4500
001 1902051
005 2024-05-02
008 2011 bl uuuu u00u1 u #d
100 1 $aMARRACCINI, P.
245 $aRBCS1 expression in coffee$bcoffea orthologs, coffea arabica homeologs, and expression variability between genotypes and under drought stress$h[electronic resource]
260 $c2011
520 $aBackground: In higher plants, the inhibition of photosynthetic capacity under drought is attributable to stomatal and non-stomatal (i.e., photochemical and biochemical) effects. In particular, a disruption of photosynthetic metabolism and Rubisco regulation can be observed. Several studies reported reduced expression of the RBCS genes, which encode the Rubisco small subunit, under water stress. Results: Expression of the RBCS1 gene was analysed in the allopolyploid context of C. arabica, which originates from a natural cross between the C. canephora and C. eugenioides species. Our study revealed the existence of two homeologous RBCS1 genes in C. arabica: one carried by the C. canephora sub-genome (called CaCc) and the other carried by the C. eugenioides sub-genome (called CaCe). Using specific primer pairs for each homeolog, expression studies revealed that CaCe was expressed in C. eugenioides and C. arabica but was undetectable in C. canephora. On the other hand, CaCc was expressed in C. canephora but almost completely silenced in non-introgressed ("pure?) genotypes of C. arabica. However, enhanced CaCc expression was observed in most C. arabica cultivars with introgressed C. canephora genome. In addition, total RBCS1 expression was higher for C. arabica cultivars that had recently introgressed C. canephora genome than for ?pure? cultivars. For both species, water stress led to an important decrease in the abundance of RBCS1 transcripts. This was observed for plants grown in either greenhouse or field conditions under severe or moderate drought. However, this reduction of RBCS1 gene expression was not accompanied by a decrease in the corresponding protein in the leaves of C. canephora subjected to water withdrawal. In that case, the amount of RBCS1 was even higher under drought than under unstressed (irrigated) conditions, which suggests great stability of RBCS1 under adverse water conditions. On the other hand, for C. arabica, high nocturnal expression of RBCS1 could also explain the accumulation of the RBCS1 protein under water stress. Altogether, the results presented here suggest that the content of RBCS was not responsible for the loss of photosynthetic capacity that is commonly observed in water-stressed coffee plants. Conclusion: We showed that the CaCe homeolog was expressed in C. eugenioides and non-introgressed ("pure?) genotypes of C. arabica but that it was undetectable in C. canephora. On the other hand, the CaCc homeolog was expressed in C. canephora but highly repressed in C. arabica. Expression of the CaCc homeolog was enhanced in C. arabica cultivars that experienced recent introgression with C. canephora. For both C. canephora and C. arabica species, total RBCS1 gene expression was highly reduced with WS. Unexpectedly, the accumulation of RBCS1 protein was observed in the leaves of C. canephora under WS, possibly coming from nocturnal RBCS1 expression. These results suggest that the increase in the amount of RBCS1 protein could contribute to the antioxidative function of photorespiration in water-stressed coffee plants.
650 $aGene expression
650 $aMolecular genetics
650 $aCafé
650 $aCoffea Arábica
650 $aCoffea Canephora
650 $aGenética Molecular
653 $aExpressão gênica
653 $aSequência genética
700 1 $aFREIRE, L. P.
700 1 $aALVES, G. S. C.
700 1 $aVIEIRA, N. G.
700 1 $aVINECKY, F.
700 1 $aELBELT, S.
700 1 $aRAMOS, H. J
700 1 $aMONTAGNON, C.
700 1 $aVIEIRA, L. G. E.
700 1 $aLEROY, T.
700 1 $aPOT, D.
700 1 $aSILVA, V. A.
700 1 $aRODRIGUES, G. C.
700 1 $aANDRADE, A. C.
773 $tBMC Plant Biology$gv. 11, n. 85, 2011.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
29/07/2003 |
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Data da última atualização: |
29/07/2003 |
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Autoria: |
ALVES, B. J. R.; BODDEY, R. M.; URQUIAGA, S. |
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Título: |
Análise de nitrato em extratos coloridos de plantas utilizando a técnica de fluxo contínuo. |
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Ano de publicação: |
1996 |
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Fonte/Imprenta: |
Seropédica: EMBRAPA-CNPAB, 1996. |
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Páginas: |
3 p. |
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Série: |
(EMBRAPA-CNPAB. Pesquisa em Andamento, 1). |
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ISSN: |
1414-1345 |
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Idioma: |
Português |
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Palavras-Chave: |
Analysis; Fluxo continuo; Methods. |
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Thesagro: |
Análise; Método; Tecido Vegetal. |
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Thesaurus NAL: |
plant tissues. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00669nam a2200241 a 4500
001 1597350
005 2003-07-29
008 1996 bl uuuu u0uu1 u #d
022 $a1414-1345
100 1 $aALVES, B. J. R.
245 $aAnálise de nitrato em extratos coloridos de plantas utilizando a técnica de fluxo contínuo.
260 $aSeropédica: EMBRAPA-CNPAB$c1996
300 $a3 p.
490 $a(EMBRAPA-CNPAB. Pesquisa em Andamento, 1).
650 $aplant tissues
650 $aAnálise
650 $aMétodo
650 $aTecido Vegetal
653 $aAnalysis
653 $aFluxo continuo
653 $aMethods
700 1 $aBODDEY, R. M.
700 1 $aURQUIAGA, S.
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Embrapa Agrobiologia (CNPAB) |
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