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| 8. |  | VITORINO, J. C.; SILLA, P. R.; CAMARGO-BRUNETTO, M. A. de.; BINNECK, E. Abordagem computacional para a identificação de elementos cis-regulatórios no genoma da soja. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 5., 2010, Londrina. Resumos... Londrina: Embrapa Soja, 2010. p. 95-97. (Embrapa Soja. Documentos, 323). Editado por Odilon Ferreira Saraiva, Paula Geron Saiz Melo.| Biblioteca(s): Embrapa Soja. |
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| 9. | | VITORINO, J. C.; SILLA, P. R.; CAMARGO-BRUNETTO, M. A. de.; BINNECK, E. Abordagem computacional para a identificação de elementos cis-regulatóriso no genoma da soja. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 5., 2010, Londrina. Resumos... Londrina: Embrapa Soja, 2010. p. 95-97. (Embrapa Soja. Documentos, 323). Editado por Odilon Ferreira Saraiva, Paula Geron Saiz Melo.| Biblioteca(s): Embrapa Soja. |
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| 10. | | MERTZ-HENNING, L. M.; NAGASHIMA, A. I.; KRZYZANOWSKI, F. C.; BINNECK, E.; HENNING, F. A. Relative quantification of gene expression levels associated with lignin biosynthesis in soybean seed coat. Seed Science and Technology, Zurich, v. 43, n. 3, p. 445-455, Dec. 2015.| Biblioteca(s): Embrapa Soja. |
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| 11. | | BUENO, P.; LAU, E. Y.; BINNECK, E.; NHANI JUNIOR, A.; SCAGLIUSI, S. M. M. RNA-Seq para análise de genes diferencialmente expressos em micrósporos de trigo. In: MOSTRA DE INICIAÇÃO CIENTÍFICA, 15.; MOSTRA DE PÓS-GRADUAÇÃO DA EMBRAPA TRIGO, 12., 2020, Passo Fundo. Resumos... Brasília, DF: Embrapa, 2021. p. 48.| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Soja. |
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| 12. | | BUENO, P.; LAU, E. Y.; BINNECK, E.; NHANI JUNIOR, A.; SCAGLIUSI, S. M. M. RNA-Seq para análise de genes diferencialmente expressos em micrósporos de trigo. In: MOSTRA DE INICIAÇÃO CIENTÍFICA, 15.; MOSTRA DE PÓS-GRADUAÇÃO DA EMBRAPA TRIGO, 12., 2020, Passo Fundo. Resumos... Brasília, DF: Embrapa, 2021. p. 48.| Biblioteca(s): Embrapa Trigo. |
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| 14. | | SOSA-GOMEZ, D. R.; CORONEL, N.; BINNECK, E.; ZUCCHI, M. I.; ROSADO-NETO, G. RAPD and mitochondrial DNA analysis of the soybean stalk weevil, Sternechus subsignatus (Coleoptera: Curculionidae. Bulletin of Entomological Research, Cambridge, v. 98, n. 5, p. 475-481, June 2008.| Biblioteca(s): Embrapa Soja. |
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| 15. | | COSTAMILAN, L.; BERTAGNOLLI, P.; YORINORI, J.; ALMEIDA, A.; SEIXAS, C.; BINNECK, E.; ARAÚJO, M.; CARBONARI, J. Soybean stem canker (Diaporthe phaseolorum var. caulivora) in Brazil. In: WORLD SOYBEAN RESEARCH CONFERENCE, 8., 2009, Beijing. Developing a global soy blueprint for a safe secure and sustainable supply: abstracts. Beijing: Chinese Academy of Agricultural Sciences: Institute of Crop Science, 2009. p. 250. Resumo P654. WSRC 2009. Editado por Lijuan Qiu, Rongxia Guan, Jian Jin, Qijan Song, Shuntang Guo, Wenbin Li, Yuanchao Wang, Tianfu Han, Xiaobing Liu, Deyue Yu, Lianzhou Jiang, Deliang Peng.| Biblioteca(s): Embrapa Trigo. |
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| 16. | | COSTAMILAN, L.; BERTAGNOLLI, P.; YORINORI, J.; ALMEIDA, A.; SEIXAS, C.; BINNECK, E.; ARAÚJO, M.; CARBONARI, J. Soybean stem canker (Diaporthe phasseolorum var. caulivora) in Brazil. In: WORLD SOYBEAN RESEARCH CONFERENCE, 8., 2009, Beijing. Developing a global soy blueprint for a safe secure and sustainable supply: abstracts. Beijing: Chinese Academy of Agricultural Sciences: Institute of Crop Science, 2009. p. 250, ref. P654. WSRC 2009. Editado por Lijuan Qiu, Rongxia Guan, Jian Jin, Qijan Song, Shuntang Guo, Wenbin Li, Yuanchao Wang, Tianfu Han, Xiaobing Liu, Deyue Yu, Lianzhou Jiang, Deliang Peng.| Biblioteca(s): Embrapa Soja. |
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| 17. | | NASCIMENTO, L. S.; SILLA, P. R.; VITORINO, J. C.; IWATA, M.; MITANI, E. A.; BINNECK, E. Soybean gene express: plataforma para análise de expressão diferencial e bibliotecas substrativas de cDNA. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 5., 2010, Londrina. Resumos... Londrina: Embrapa Soja, 2010. p. 105-109. (Embrapa Soja. Documentos, 323). Editado por Odilon Ferreira Saraiva, Paula Geron Saiz Melo.| Biblioteca(s): Embrapa Soja. |
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| 18. | | TURCHETTO, C.; GONZÁLES, H. H. S.; TORRES, G. A. M.; NHANI, A.; CONSOLI, L.; BINNECK, E. Suppression subtractive hybridization analysis of genes regulated by Magnaporthe oryzae infection in wheat adult plants. In: CONGRESSO BRASILEIRO DE GENÉTICA, 61., 2015, Águas de Lindóia. Pós-genômica: [resumos]. [Ribeirão Preto]: Sociedade Brasileira de Genética, [2015]. p. 34.| Biblioteca(s): Embrapa Trigo. |
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| Registros recuperados : 176 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Trigo. |
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Data corrente: |
17/03/2017 |
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Data da última atualização: |
21/08/2019 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
TURCHETTO, C.; GONZÁLES, H. H. S.; TORRES, G. A. M.; NHANI, A.; CONSOLI, L.; BINNECK, E. |
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Afiliação: |
GISELE ABIGAIL MONTAN TORRES, CNPT; ANTONIO NHANI JUNIOR, CNPT; LUCIANO CONSOLI, CNPT; ELISEU BINNECK, CNPSO. |
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Título: |
Suppression subtractive hybridization analysis of genes regulated by Magnaporthe oryzae infection in wheat adult plants. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE GENÉTICA, 61., 2015, Águas de Lindóia. Pós-genômica: [resumos]. [Ribeirão Preto]: Sociedade Brasileira de Genética, [2015]. |
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Páginas: |
p. 34. |
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Idioma: |
Inglês |
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Conteúdo: |
Blast (also known as brusone), caused by the fungus Magnaporthe oryzae, is a serious disease of wheat (Triticum aestivum) across central and southern Brazil. The pathogen is a hemibiotrophic ascomycete that attacks several grass species. The disease was first described in rice in 1600 in China and it was reported infecting wheat ears in 1985 in Paraná state, Brazil, and since spread to all growing-regions in the country. Currently has been also reported on wheat fields in Bolivia, Paraguay and Argentina. The rice blast disease has emerged as a model for the study of phytopathogenic fungi showing that this pathogen initially colonizes host tissues as a biotroph, without causing detectable symptoms. Approximately 72?96 h after infection, lesions become apparent in the plant, characterizing the necrotrophic growth of M. oryzae. In wheat plants, depending on the developmental stage at which infection occurs blast can be devastating. Infected heads produce small and wrinkled grains with low specific weight. Few cultivars are described as resistant to wheat blast and fungicides have low control efficiency of the disease. Little is known about the molecular mechanism of wheat resistance to the pathogen. Here, we investigated the responses of wheat to M. oryzae infection in reproductive stage at 40 h after inoculation. The aim of this study was to identify genes that are differentially up- or downregulated in adults plants of Triticum aestivum infected with Magnaporthe oryzae. For this, we used a suppression subtractive hybridization (SSH) approach. A total of 420 high-quality contigs were isolated, 415 of them were mapped in Triticum aestivum genome. The 420 contigs were searched against the non-redundant nucleotide and protein databases in GenBank to predict the function for the corresponding genes. Fifty-five contigs corresponded to defense-related genes. We used the quantitative RT-PCR analysis to validate the differential expression patterns for 16 Triticum aestivum genes between control and inoculated spikes. Nine genes presented higher transcript levels under inoculation, including one gene previously described as responsive to Magnaporthe infection on wheat seedlings. This gene coding one protein membrane-associated that may increase the adhesion of the plasma membrane to the cell wall during pathogen infection. In contrast, the other 7 genes presented higher expression in mock-inoculated spikes. The study of these genes and the associated defense mechanisms can provide a significant advance in our understanding of the putative determinants of the resistance mechanisms of this wheat resistant genotype. MenosBlast (also known as brusone), caused by the fungus Magnaporthe oryzae, is a serious disease of wheat (Triticum aestivum) across central and southern Brazil. The pathogen is a hemibiotrophic ascomycete that attacks several grass species. The disease was first described in rice in 1600 in China and it was reported infecting wheat ears in 1985 in Paraná state, Brazil, and since spread to all growing-regions in the country. Currently has been also reported on wheat fields in Bolivia, Paraguay and Argentina. The rice blast disease has emerged as a model for the study of phytopathogenic fungi showing that this pathogen initially colonizes host tissues as a biotroph, without causing detectable symptoms. Approximately 72?96 h after infection, lesions become apparent in the plant, characterizing the necrotrophic growth of M. oryzae. In wheat plants, depending on the developmental stage at which infection occurs blast can be devastating. Infected heads produce small and wrinkled grains with low specific weight. Few cultivars are described as resistant to wheat blast and fungicides have low control efficiency of the disease. Little is known about the molecular mechanism of wheat resistance to the pathogen. Here, we investigated the responses of wheat to M. oryzae infection in reproductive stage at 40 h after inoculation. The aim of this study was to identify genes that are differentially up- or downregulated in adults plants of Triticum aestivum infected with Magnaporthe oryzae. For t... Mostrar Tudo |
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Thesagro: |
Trigo. |
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Thesaurus NAL: |
Wheat. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/135952/1/suppression.binneck.2015.pdf
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Marc: |
LEADER 03393naa a2200217 a 4500
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008 2015 bl --- 0-- u #d
100 1 $aTURCHETTO, C.
245 $aSuppression subtractive hybridization analysis of genes regulated by Magnaporthe oryzae infection in wheat adult plants.$h[electronic resource]
260 $c2015
300 $ap. 34.
520 $aBlast (also known as brusone), caused by the fungus Magnaporthe oryzae, is a serious disease of wheat (Triticum aestivum) across central and southern Brazil. The pathogen is a hemibiotrophic ascomycete that attacks several grass species. The disease was first described in rice in 1600 in China and it was reported infecting wheat ears in 1985 in Paraná state, Brazil, and since spread to all growing-regions in the country. Currently has been also reported on wheat fields in Bolivia, Paraguay and Argentina. The rice blast disease has emerged as a model for the study of phytopathogenic fungi showing that this pathogen initially colonizes host tissues as a biotroph, without causing detectable symptoms. Approximately 72?96 h after infection, lesions become apparent in the plant, characterizing the necrotrophic growth of M. oryzae. In wheat plants, depending on the developmental stage at which infection occurs blast can be devastating. Infected heads produce small and wrinkled grains with low specific weight. Few cultivars are described as resistant to wheat blast and fungicides have low control efficiency of the disease. Little is known about the molecular mechanism of wheat resistance to the pathogen. Here, we investigated the responses of wheat to M. oryzae infection in reproductive stage at 40 h after inoculation. The aim of this study was to identify genes that are differentially up- or downregulated in adults plants of Triticum aestivum infected with Magnaporthe oryzae. For this, we used a suppression subtractive hybridization (SSH) approach. A total of 420 high-quality contigs were isolated, 415 of them were mapped in Triticum aestivum genome. The 420 contigs were searched against the non-redundant nucleotide and protein databases in GenBank to predict the function for the corresponding genes. Fifty-five contigs corresponded to defense-related genes. We used the quantitative RT-PCR analysis to validate the differential expression patterns for 16 Triticum aestivum genes between control and inoculated spikes. Nine genes presented higher transcript levels under inoculation, including one gene previously described as responsive to Magnaporthe infection on wheat seedlings. This gene coding one protein membrane-associated that may increase the adhesion of the plasma membrane to the cell wall during pathogen infection. In contrast, the other 7 genes presented higher expression in mock-inoculated spikes. The study of these genes and the associated defense mechanisms can provide a significant advance in our understanding of the putative determinants of the resistance mechanisms of this wheat resistant genotype.
650 $aWheat
650 $aTrigo
700 1 $aGONZÁLES, H. H. S.
700 1 $aTORRES, G. A. M.
700 1 $aNHANI, A.
700 1 $aCONSOLI, L.
700 1 $aBINNECK, E.
773 $tIn: CONGRESSO BRASILEIRO DE GENÉTICA, 61., 2015, Águas de Lindóia. Pós-genômica: [resumos]. [Ribeirão Preto]: Sociedade Brasileira de Genética, [2015].
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