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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
16/06/2011 |
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Data da última atualização: |
27/07/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MINGOTI, G. Z.; CASTRO, V. S. D. C.; NICIURA, S. C. M.; BARRETO, L. S. S.; GARCIA, J. M. |
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Afiliação: |
GISELE ZOCCAL MINGOTI, UNESP/ARAÇATUBA; VIVIANE SGGOBI DIAS CAIADO CASTRO, UNESP/JABOTICABAL; SIMONE CRISTINA MEO NICIURA, CPPSE; LETÍCIA SIQUEIRA SÁ BARETTO, UNESP/JABOTICABAL; JOAQUIM MANSANO GARCIA, UNESP/JABOTICABAL. |
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Título: |
The effects of macromolecular and serum supplements and oxygen tension during bovine in vitro procedures on kinetics of oocyte maturation and embryo development. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In Vitro Cell Dev Biol Anim., v. 47, n. 5-6, p.361-367, jun. 2011. |
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DOI: |
10.1007/s11626-011-9400-0 |
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Idioma: |
Inglês |
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Conteúdo: |
Aiming to standardize in vitro production of bovine embryos and to obtain supplements to replace serum in culture media, this study evaluated the nuclear maturation kinetics and embryonic development in bovine after in vitro maturation (IVM) and culture (IVC) with several macromolecules (animal origin: bovine serum albumin (BSA), fetal calf serum (FCS); synthetic: polyvinyl alcohol (PVA), polyvinyl pyrrolidone (PVP), Ficoll, and Knockout) at two oxygen tensions (20% and 5% O2). Regarding nuclear kinetics, neither the presence of the expected stage (metaphase I, transition anaphase to telophase, and metaphase II) at each evaluation moment (6, 18, and 24 h after IVM, respectively) nor the accelerated polar body emission (at 18 h after IVM) related developmental competence to blastocyst stage when different supplements were compared. Independently of supplement, cleavage rates at 20% O2 (61.6?79.2%) were higher than at 5% O2 (38.9?58.7%). At 20% O2, higher blastocyst and hatching rates, respectively, were obtained in treatments BSA, FCS, Knockout, and control group (IVM with FCS and IVC with BSA + FCS, 14.0?23.5% and 6.8?15.4%) in comparison to PVA, PVP, and Ficoll (0%). The same was observed at 5% O2 for blastocyst rates with BSA, FCS, Knockout, and control (5.4?16.8%) and for hatching rates with BSA, FCS, and control (2.0?11.1%). We can conclude that producing bovine embryos at 20% O2 during the entire IVP process resulted in higher developmental rates than at 5% O2. In addition, while defined macromolecules PVA, PVP, and Ficoll were not suitable for embryonic development, the synthetic serum Knockout was able to replace serum and albumin for IVP in bovine at 20% O2. MenosAiming to standardize in vitro production of bovine embryos and to obtain supplements to replace serum in culture media, this study evaluated the nuclear maturation kinetics and embryonic development in bovine after in vitro maturation (IVM) and culture (IVC) with several macromolecules (animal origin: bovine serum albumin (BSA), fetal calf serum (FCS); synthetic: polyvinyl alcohol (PVA), polyvinyl pyrrolidone (PVP), Ficoll, and Knockout) at two oxygen tensions (20% and 5% O2). Regarding nuclear kinetics, neither the presence of the expected stage (metaphase I, transition anaphase to telophase, and metaphase II) at each evaluation moment (6, 18, and 24 h after IVM, respectively) nor the accelerated polar body emission (at 18 h after IVM) related developmental competence to blastocyst stage when different supplements were compared. Independently of supplement, cleavage rates at 20% O2 (61.6?79.2%) were higher than at 5% O2 (38.9?58.7%). At 20% O2, higher blastocyst and hatching rates, respectively, were obtained in treatments BSA, FCS, Knockout, and control group (IVM with FCS and IVC with BSA + FCS, 14.0?23.5% and 6.8?15.4%) in comparison to PVA, PVP, and Ficoll (0%). The same was observed at 5% O2 for blastocyst rates with BSA, FCS, Knockout, and control (5.4?16.8%) and for hatching rates with BSA, FCS, and control (2.0?11.1%). We can conclude that producing bovine embryos at 20% O2 during the entire IVP process resulted in higher developmental rates than at 5% O2. In addit... Mostrar Tudo |
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Palavras-Chave: |
Embryo; In vitro maturation; Macromolecule. |
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Thesaurus Nal: |
beef cattle; oxygen. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02490naa a2200241 a 4500
001 1894336
005 2022-07-27
008 2011 bl uuuu u00u1 u #d
024 7 $a10.1007/s11626-011-9400-0$2DOI
100 1 $aMINGOTI, G. Z.
245 $aThe effects of macromolecular and serum supplements and oxygen tension during bovine in vitro procedures on kinetics of oocyte maturation and embryo development.$h[electronic resource]
260 $c2011
520 $aAiming to standardize in vitro production of bovine embryos and to obtain supplements to replace serum in culture media, this study evaluated the nuclear maturation kinetics and embryonic development in bovine after in vitro maturation (IVM) and culture (IVC) with several macromolecules (animal origin: bovine serum albumin (BSA), fetal calf serum (FCS); synthetic: polyvinyl alcohol (PVA), polyvinyl pyrrolidone (PVP), Ficoll, and Knockout) at two oxygen tensions (20% and 5% O2). Regarding nuclear kinetics, neither the presence of the expected stage (metaphase I, transition anaphase to telophase, and metaphase II) at each evaluation moment (6, 18, and 24 h after IVM, respectively) nor the accelerated polar body emission (at 18 h after IVM) related developmental competence to blastocyst stage when different supplements were compared. Independently of supplement, cleavage rates at 20% O2 (61.6?79.2%) were higher than at 5% O2 (38.9?58.7%). At 20% O2, higher blastocyst and hatching rates, respectively, were obtained in treatments BSA, FCS, Knockout, and control group (IVM with FCS and IVC with BSA + FCS, 14.0?23.5% and 6.8?15.4%) in comparison to PVA, PVP, and Ficoll (0%). The same was observed at 5% O2 for blastocyst rates with BSA, FCS, Knockout, and control (5.4?16.8%) and for hatching rates with BSA, FCS, and control (2.0?11.1%). We can conclude that producing bovine embryos at 20% O2 during the entire IVP process resulted in higher developmental rates than at 5% O2. In addition, while defined macromolecules PVA, PVP, and Ficoll were not suitable for embryonic development, the synthetic serum Knockout was able to replace serum and albumin for IVP in bovine at 20% O2.
650 $abeef cattle
650 $aoxygen
653 $aEmbryo
653 $aIn vitro maturation
653 $aMacromolecule
700 1 $aCASTRO, V. S. D. C.
700 1 $aNICIURA, S. C. M.
700 1 $aBARRETO, L. S. S.
700 1 $aGARCIA, J. M.
773 $tIn Vitro Cell Dev Biol Anim.$gv. 47, n. 5-6, p.361-367, jun. 2011.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
21/07/2022 |
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Data da última atualização: |
25/07/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
COSTA, A. R.; SILVA, J. R. de L.; PEREIRA, P. S.; BEZERRA, J. W. A.; OLIVEIRA, M. A. S. de; RIBEIRO, P. R. V.; BRITO, E. S. de; DRUMOND, M. A.; CALIXTO JÚNIOR, J. T.; KAMDEM, J. P.; DUARTE, A. E.; BARROS, L. M.; COUTINHO, H. D. M. |
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Afiliação: |
ADRIELLE RODRIGUES COSTA, Researcher at the Structural and Molecular Biology Laboratory, Center for Agricultural Sciences and Biodiversity Federal University of Cariri, Crato, CE; JAILSON RENATO DE LIMA SILVA, Researcher at the Biology and Toxicology Laboratory of the Regional University of Cariri, Crato, CE; PEDRO SILVINO PEREIRA, Researcher at the Biology and Toxicology Laboratory of the Regional University of Cariri, Crato, CE; JOSÉ WEVERTON ALMEIDA BEZERRA, Researcher at the Biology and Toxicology Laboratory of the Regional University of Cariri, Crato, CE; MARIA AUREA SOARES DE OLIVEIRA, Researcher at the Biology and Toxicology Laboratory of the Regional University of Cariri, Crato, CE; PAULO RICELI VASCONCELOS RIBEIRO, CNPAT; EDY SOUSA DE BRITO, CNAT; MARCOS ANTONIO DRUMOND, CPATSA; JOÃO TAVARES CALIXTO JÚNIOR, Department of Biological Sciences, University of Regional Cariri (URCA), Crato, CE; JEAN PAUL KAMDEM, Researcher at the Biology and Toxicology Laboratory of the Regional University of Cariri, Crato, CE; ANTONIA ELIENE DUARTE, Researcher at the Biology and Toxicology Laboratory of the Regional University of Cariri, Crato, CE; LUIZ MARIVANDO BARROS, Department of Biological Sciences, University of Regional Cariri (URCA), Crato, CE; HENRIQUE DOUGLAS MELO COUTINHO, Researcher at the Laboratory of Microbiology and Molecular Biology, Regional University of Cariri, Crato, CE. |
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Título: |
Influence of abiotic factors on phytochemical diversity of Anacardium occidentale L. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Food Bioscience, v. 49, 101911, 2022. |
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DOI: |
https://doi.org/10.1016/j.fbio.2022.101911 |
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Idioma: |
Inglês |
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Conteúdo: |
To understand the influence of environmental factors on the metabolic diversity of phytochemical compounds from extracts of leaves and bark of Anacardium occidentale, collected in two different geographic areas, ultra- performance liquid chromatography and ass spectrometry were used, as well as the composition of physico-chemical properties of both soils at the collection site (?Lavras da Mangabeira? and ?Crato?). It was possible to observe physicochemical differences between the soils of the two collection areas and some similarities. Both present granulometry with a sandy texture, with high values of total sand, with acid pH, and variation in porosity rates. The micronutrients with the highest accumulation in the soil, with emphasis on the predominance of P (Phosphorus) and Al (Aluminium) mainly in the 2040 cm layer in Crato. Thirty-three phytochemical compounds were identified in the leaf and bark extracts, of which 28 were present in the leaves and 20 in the bark part. Of this total, 13 were present in at least one of the parts of the plant (leaf or bark), and most were present in both Crato and Lavras da Mangabeira, presenting mainly phenolic acids, flavonoids and tannins in all extracts eval- uated. Both extracts showed similarities between the groups of compounds. Taken together, this study may be relevant to assess environmental influences on the production of secondary metabolites. |
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Palavras-Chave: |
Cajueiro; Composto fitoquímico; Diversidade fitoquímica; Fatores abióticos. |
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Thesagro: |
Anacardiaceae; Caju; Composto Químico; Extrato de Fruta; Metabolismo Vegetal. |
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Thesaurus NAL: |
Cashew fruit. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02565naa a2200397 a 4500
001 2144847
005 2022-07-25
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.fbio.2022.101911$2DOI
100 1 $aCOSTA, A. R.
245 $aInfluence of abiotic factors on phytochemical diversity of Anacardium occidentale L.$h[electronic resource]
260 $c2022
520 $aTo understand the influence of environmental factors on the metabolic diversity of phytochemical compounds from extracts of leaves and bark of Anacardium occidentale, collected in two different geographic areas, ultra- performance liquid chromatography and ass spectrometry were used, as well as the composition of physico-chemical properties of both soils at the collection site (?Lavras da Mangabeira? and ?Crato?). It was possible to observe physicochemical differences between the soils of the two collection areas and some similarities. Both present granulometry with a sandy texture, with high values of total sand, with acid pH, and variation in porosity rates. The micronutrients with the highest accumulation in the soil, with emphasis on the predominance of P (Phosphorus) and Al (Aluminium) mainly in the 2040 cm layer in Crato. Thirty-three phytochemical compounds were identified in the leaf and bark extracts, of which 28 were present in the leaves and 20 in the bark part. Of this total, 13 were present in at least one of the parts of the plant (leaf or bark), and most were present in both Crato and Lavras da Mangabeira, presenting mainly phenolic acids, flavonoids and tannins in all extracts eval- uated. Both extracts showed similarities between the groups of compounds. Taken together, this study may be relevant to assess environmental influences on the production of secondary metabolites.
650 $aCashew fruit
650 $aAnacardiaceae
650 $aCaju
650 $aComposto Químico
650 $aExtrato de Fruta
650 $aMetabolismo Vegetal
653 $aCajueiro
653 $aComposto fitoquímico
653 $aDiversidade fitoquímica
653 $aFatores abióticos
700 1 $aSILVA, J. R. de L.
700 1 $aPEREIRA, P. S.
700 1 $aBEZERRA, J. W. A.
700 1 $aOLIVEIRA, M. A. S. de
700 1 $aRIBEIRO, P. R. V.
700 1 $aBRITO, E. S. de
700 1 $aDRUMOND, M. A.
700 1 $aCALIXTO JÚNIOR, J. T.
700 1 $aKAMDEM, J. P.
700 1 $aDUARTE, A. E.
700 1 $aBARROS, L. M.
700 1 $aCOUTINHO, H. D. M.
773 $tFood Bioscience$gv. 49, 101911, 2022.
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