Data/hora: 20/06/2024 20:32:43

Biblioteca(s): | Embrapa Meio Ambiente.
Data corrente: | 26/07/2018
Data da última atualização: | 26/07/2018
Autoria: | CARVALHO, A. L. de A.; REZENDE, L. C. de; COSTA, L. B.; HALFELD-VIEIRA, B. de A.; PINTO, Z. V.; MORANDI, M. A. B.; MEDEIROS, F. H. V.; BETTIOL, W.
Afiliação: | ANDRE LUIZ DE ANDRADE CARVALHO, UFV; LARISSA CASTRO DE REZENDE, UFV; LUCIO BERTOLDO COSTA, FCA-UNESP; BERNARDO DE ALMEIDA HALFELD VIEIRA, CNPMA; ZAYAME VEGETTE PINTO, FCA-UNESP; MARCELO AUGUSTO BOECHAT MORANDI, CNPMA; FLAVIO HENRIQUE VASCONCELOS DE MEDEIROS, UFV; WAGNER BETTIOL, CNPMA.
Título: | Optimizing the mass production of Clonostachys rosea by liquid-state fermentation.
Ano de publicação: | 2018
Fonte/Imprenta: | Biological Control, v. 118, p. 16-25, 2018.
DOI: | https://doi.org/10.1016/j.biocontrol.2017.11.014
Idioma: | Inglês
Conteúdo: | Abstract: Optimization of the culture medium and the entire mass production process for various fungi in liquid medium has been studied. However, the technology is not well developed for Clonostachys rosea, a biocontrol agent against various plant pathogens due to its antagonistic capacity to act as a hyperparasite, compete for nutrients and space, and induce plant resistance to pathogens. In this study, we aimed to optimize the culture medium and to standardize parameters that may interfere with the production of C. rosea conidia in a liquid-state fermentation system. Culturing was performed in 250-mL Erlenmeyer flasks shaken for 7 days, followed by planned experimental methodology to reduce the number of analyses and consumable costs. Benchtop bioreactor tests with the optimized medium were performed. Glucose and sucrose were evaluated as carbon sources. Initially, the effects of temperature, pH, photoperiod, carbon:nitrogen ratio and water activity on inoculum production were evaluated, with the pH and photoperiod being factors that contributed to conidial production. Optimization of the fermentation conditions was performed using a central composite rotational design (CCD) with a wider range of pH values and photoperiods. The remaining variables were fixed according to the previous assay. Colony-forming unit (CFUs), biomass production and conidial viability were evaluated, and glucose was used as a carbon source to enhance conidial production. The optimized conditions that resulted in a maximum yield of conidia (1.78 x 107 conidia?mL-1), dried biomass (0.558?g) and CFUs (5.15 x 106 CFUs?mL-1) were a pH value of 4 and a photoperiod of 12?h.
Palavras-Chave: | Bioreactor; Liquid media; Produção massal.
Thesagro: | Fungo Para Controle Biológico.
Thesaurus Nal: | Biological control agents; Biomass production; Bioreactors; Clonostachys rosea.
Categoria do assunto: | H Saúde e Patologia
Registro original: | Embrapa Meio Ambiente (CNPMA)
Exemplares: | CNPMA - (16144-1) - UPC - AP - DD -  -  -  - DIS