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Registro Completo |
Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
Data corrente: |
25/02/2008 |
Data da última atualização: |
21/10/2015 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
RANGEL, M. S. A.; TUPINAMBÁ, E. A.; AMARAL, G. H. |
Afiliação: |
Maria Salete Alves RangelSecretaria Executiva de Agricultura Irrigação pesca e Abastecimento de Alagoas/Embrapa Tabuleiros Costeiros; Evandro Almeida Tupinambá, Embrapa Tabuleiros Costeiros; Gizeli Hora Amaral, (estagiária) Embrapa Tabuleiros Costeiros. |
Título: |
Identificação de espécies arbóreas e arbustivas da Reserva Ambiental do Caju para implatação de programas de educação ambiental e estratégias de conservação. |
Ano de publicação: |
2007 |
Fonte/Imprenta: |
In: CONGRESSO NACIONAL DE BOTÂNICA, 58., 2007, São Paulo. Anais... São Paulo: Sociedade Botânica do Brasil, 2007. |
Idioma: |
Português |
Palavras-Chave: |
Floresta Atlantica; Resrva Ambiental do Caju. |
Thesagro: |
Biodiversidade. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/131504/1/Identificacao0001.pdf
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Marc: |
LEADER 00667nam a2200157 a 4500 001 1372375 005 2015-10-21 008 2007 bl uuuu u00u1 u #d 100 1 $aRANGEL, M. S. A. 245 $aIdentificação de espécies arbóreas e arbustivas da Reserva Ambiental do Caju para implatação de programas de educação ambiental e estratégias de conservação.$h[electronic resource] 260 $aIn: CONGRESSO NACIONAL DE BOTÂNICA, 58., 2007, São Paulo. Anais... São Paulo: Sociedade Botânica do Brasil$c2007 650 $aBiodiversidade 653 $aFloresta Atlantica 653 $aResrva Ambiental do Caju 700 1 $aTUPINAMBÁ, E. A. 700 1 $aAMARAL, G. H.
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Embrapa Tabuleiros Costeiros (CPATC) |
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Registro Completo
Biblioteca(s): |
Embrapa Florestas. |
Data corrente: |
31/03/2017 |
Data da última atualização: |
13/12/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
OLIVEIRA, C. de; DEGENHARDT-GOLDBACH, J.; BETTENCOURT, G. M. de F.; AMANO, E.; FRANCISCON, L.; QUOIRIN, M. |
Afiliação: |
CASSIANA DE OLIVEIRA, UFPR; JULIANA DEGENHARDT GOLDBACH, CNPF; GISELA MANUELA DE FRANÇA BETTENCOURT; ERIKA AMANO, UFPR; LUZIANE FRANCISCON, CNPF; MARGUERITE QUOIRIN, UFPR. |
Título: |
Micropropagation of Eucalyptus grandis X E. urophylla AEC 224 clone. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Journal of Forestry Research, v. 28, n. 1, p. 29-39, Jan. 2017. |
DOI: |
10.1007/s11676-016-0282-6 |
Idioma: |
Inglês |
Conteúdo: |
Genetic transformation systems require protocols that allow regenerating transgenic plants from transformed tissues. This study aimed to establish a protocol for indirect organogenesis in leaf explants of a Eucalyptus grandis 3 E. urophylla AEC 224 clone. During callogenesis stage, several concentrations of NAA and then NAA or 2,4-D combined with TDZ were tested in JADS culture medium for 30 days, followed by subculture of the explants in the regeneration medium, containing 5.0 lM BA and 0.5 lM NAA for another 30 days. In these media, the explant oxidation rate was high (95 %). Thus, in order to reduce oxidation, different culture media were compared: WPM, MS, JADS and modified QL, followed by explant transfer onto regeneration medium. The highest percentage of regeneration and the lowest oxidation rate were achieved on WPM medium. Then, NAA and 2,4-D were tested in combination with TDZ and also TDZ and BA combined with NAA in WPM medium. The most efficient culture media in terms of shoot regeneration were WPM supplemented with 0.25 lM TDZ and 0.1 lM NAA during 30 days for callus induction and then with 5.0 lM BA and 0.5 lM NAA for another 30 days. This protocol yielded a regeneration rate of 43 %, with a low oxidation of tissues. A rooting experiment was conducted using half strength MS medium and comparing three concentrations of IBA (2.46, 4.90 and 7.35 lM). The highest rooting percentage (35 %) was obtained on medium containing 2.46 lM IBA. Once the shoots were rooted, acclimatization in a greenhouse was not challenging and plant survival reached 100 %. MenosGenetic transformation systems require protocols that allow regenerating transgenic plants from transformed tissues. This study aimed to establish a protocol for indirect organogenesis in leaf explants of a Eucalyptus grandis 3 E. urophylla AEC 224 clone. During callogenesis stage, several concentrations of NAA and then NAA or 2,4-D combined with TDZ were tested in JADS culture medium for 30 days, followed by subculture of the explants in the regeneration medium, containing 5.0 lM BA and 0.5 lM NAA for another 30 days. In these media, the explant oxidation rate was high (95 %). Thus, in order to reduce oxidation, different culture media were compared: WPM, MS, JADS and modified QL, followed by explant transfer onto regeneration medium. The highest percentage of regeneration and the lowest oxidation rate were achieved on WPM medium. Then, NAA and 2,4-D were tested in combination with TDZ and also TDZ and BA combined with NAA in WPM medium. The most efficient culture media in terms of shoot regeneration were WPM supplemented with 0.25 lM TDZ and 0.1 lM NAA during 30 days for callus induction and then with 5.0 lM BA and 0.5 lM NAA for another 30 days. This protocol yielded a regeneration rate of 43 %, with a low oxidation of tissues. A rooting experiment was conducted using half strength MS medium and comparing three concentrations of IBA (2.46, 4.90 and 7.35 lM). The highest rooting percentage (35 %) was obtained on medium containing 2.46 lM IBA. Once the shoots were rooted, a... Mostrar Tudo |
Palavras-Chave: |
Callogenesis; Eucalyptus urograndis; WPM culture medium. |
Thesagro: |
Micropropagação; Organogênese; Propagação vegetativa. |
Thesaurus NAL: |
Callus formation; Micropropagation; Organogenesis; Plant propagation. |
Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
Marc: |
LEADER 02512naa a2200313 a 4500 001 2067974 005 2017-12-13 008 2017 bl uuuu u00u1 u #d 024 7 $a10.1007/s11676-016-0282-6$2DOI 100 1 $aOLIVEIRA, C. de 245 $aMicropropagation of Eucalyptus grandis X E. urophylla AEC 224 clone.$h[electronic resource] 260 $c2017 520 $aGenetic transformation systems require protocols that allow regenerating transgenic plants from transformed tissues. This study aimed to establish a protocol for indirect organogenesis in leaf explants of a Eucalyptus grandis 3 E. urophylla AEC 224 clone. During callogenesis stage, several concentrations of NAA and then NAA or 2,4-D combined with TDZ were tested in JADS culture medium for 30 days, followed by subculture of the explants in the regeneration medium, containing 5.0 lM BA and 0.5 lM NAA for another 30 days. In these media, the explant oxidation rate was high (95 %). Thus, in order to reduce oxidation, different culture media were compared: WPM, MS, JADS and modified QL, followed by explant transfer onto regeneration medium. The highest percentage of regeneration and the lowest oxidation rate were achieved on WPM medium. Then, NAA and 2,4-D were tested in combination with TDZ and also TDZ and BA combined with NAA in WPM medium. The most efficient culture media in terms of shoot regeneration were WPM supplemented with 0.25 lM TDZ and 0.1 lM NAA during 30 days for callus induction and then with 5.0 lM BA and 0.5 lM NAA for another 30 days. This protocol yielded a regeneration rate of 43 %, with a low oxidation of tissues. A rooting experiment was conducted using half strength MS medium and comparing three concentrations of IBA (2.46, 4.90 and 7.35 lM). The highest rooting percentage (35 %) was obtained on medium containing 2.46 lM IBA. Once the shoots were rooted, acclimatization in a greenhouse was not challenging and plant survival reached 100 %. 650 $aCallus formation 650 $aMicropropagation 650 $aOrganogenesis 650 $aPlant propagation 650 $aMicropropagação 650 $aOrganogênese 650 $aPropagação vegetativa 653 $aCallogenesis 653 $aEucalyptus urograndis 653 $aWPM culture medium 700 1 $aDEGENHARDT-GOLDBACH, J. 700 1 $aBETTENCOURT, G. M. de F. 700 1 $aAMANO, E. 700 1 $aFRANCISCON, L. 700 1 $aQUOIRIN, M. 773 $tJournal of Forestry Research$gv. 28, n. 1, p. 29-39, Jan. 2017.
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