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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
09/11/2021 |
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Data da última atualização: |
09/11/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SOARES, I. C.; PACHECO, R. S.; SILVA, C. G. N. da; SANTOS, R. S.; BALDANI, J. I.; URQUIAGA, S.; VIDAL, M. S.; ARAUJO, J. L. S. de. |
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Afiliação: |
ISIS CAPELLA SOARES, UFRRJ; RAFAEL SANCHES PACHECO, UFRRJ; CLEUDISON GABRIEL NASCIMENTO DA SILVA, UFLA; RAFAEL SALAZAR SANTOS, UFRRJ; JOSE IVO BALDANI, CNPAB; SEGUNDO SACRAMENTO U CABALLERO, CNPAB; MARCIA SOARES VIDAL, CNPAB; JEAN LUIZ SIMOES DE ARAUJO, CNPAB. |
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Título: |
Real-time PCR method to quantify Sp245 strain of Azospirillum baldaniorum on Brachiaria grasses under field conditions. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Plant and Soil, 06 September 2021 |
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ISSN: |
0032-079X |
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DOI: |
https://doi.org/10.1007/s11104-021-05137-y |
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Idioma: |
Inglês |
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Conteúdo: |
Bacterial quantification by qPCR is considered the gold standard for microbial molecular diagnosis. However, a fundamental pre-requisite in this methodology is the designing of specific primers for the bacterium of interest. With the increase in bacterial genome sequencing data in the recent years, it has become possible to design specific primers that can be used to quantify different strains of the same bacterial species. Methods: To develop a real-time PCR (qPCR) protocol for the specific quantification of Azospirillum baldaniorum Sp245 strain (old Azospirillum brasilense), the Sp245 genome sequence was fragmented into small contigs with 500 base pairs each, and analyzed for similarity against the NCBI non-redundant database. A. baldaniorum-specific contigs were used to design the primers. The best pair of primers was used to quantify these bacteria after inoculation in different cultivars of Brachiaria, grown under field conditions. Results: Our results showed that the primer pair Sp245p10 was highly specific for the Sp245 strain in the Brachiaria root and shoot field under different conditions. The qPCR assay using these primers showed differences among cultivars in the number of bacteria detected in plants after inoculation. Additionally, the number of bacteria observed in the roots was higher than that in the shoots. Conclusion: The qPCR methodology using a Sp245 strain-specific primer may be used to monitor A. baldaniorum inoculated into other plants and may find potential application in field experiments. MenosBacterial quantification by qPCR is considered the gold standard for microbial molecular diagnosis. However, a fundamental pre-requisite in this methodology is the designing of specific primers for the bacterium of interest. With the increase in bacterial genome sequencing data in the recent years, it has become possible to design specific primers that can be used to quantify different strains of the same bacterial species. Methods: To develop a real-time PCR (qPCR) protocol for the specific quantification of Azospirillum baldaniorum Sp245 strain (old Azospirillum brasilense), the Sp245 genome sequence was fragmented into small contigs with 500 base pairs each, and analyzed for similarity against the NCBI non-redundant database. A. baldaniorum-specific contigs were used to design the primers. The best pair of primers was used to quantify these bacteria after inoculation in different cultivars of Brachiaria, grown under field conditions. Results: Our results showed that the primer pair Sp245p10 was highly specific for the Sp245 strain in the Brachiaria root and shoot field under different conditions. The qPCR assay using these primers showed differences among cultivars in the number of bacteria detected in plants after inoculation. Additionally, the number of bacteria observed in the roots was higher than that in the shoots. Conclusion: The qPCR methodology using a Sp245 strain-specific primer may be used to monitor A. baldaniorum inoculated into other plants and may find pot... Mostrar Tudo |
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Palavras-Chave: |
Azospirillum baldanioru; Plant growth promoting bacteria; Quantification; Signal grass. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02397naa a2200277 a 4500
001 2135952
005 2021-11-09
008 2021 bl uuuu u00u1 u #d
022 $a0032-079X
024 7 $ahttps://doi.org/10.1007/s11104-021-05137-y$2DOI
100 1 $aSOARES, I. C.
245 $aReal-time PCR method to quantify Sp245 strain of Azospirillum baldaniorum on Brachiaria grasses under field conditions.$h[electronic resource]
260 $c2021
520 $aBacterial quantification by qPCR is considered the gold standard for microbial molecular diagnosis. However, a fundamental pre-requisite in this methodology is the designing of specific primers for the bacterium of interest. With the increase in bacterial genome sequencing data in the recent years, it has become possible to design specific primers that can be used to quantify different strains of the same bacterial species. Methods: To develop a real-time PCR (qPCR) protocol for the specific quantification of Azospirillum baldaniorum Sp245 strain (old Azospirillum brasilense), the Sp245 genome sequence was fragmented into small contigs with 500 base pairs each, and analyzed for similarity against the NCBI non-redundant database. A. baldaniorum-specific contigs were used to design the primers. The best pair of primers was used to quantify these bacteria after inoculation in different cultivars of Brachiaria, grown under field conditions. Results: Our results showed that the primer pair Sp245p10 was highly specific for the Sp245 strain in the Brachiaria root and shoot field under different conditions. The qPCR assay using these primers showed differences among cultivars in the number of bacteria detected in plants after inoculation. Additionally, the number of bacteria observed in the roots was higher than that in the shoots. Conclusion: The qPCR methodology using a Sp245 strain-specific primer may be used to monitor A. baldaniorum inoculated into other plants and may find potential application in field experiments.
653 $aAzospirillum baldanioru
653 $aPlant growth promoting bacteria
653 $aQuantification
653 $aSignal grass
700 1 $aPACHECO, R. S.
700 1 $aSILVA, C. G. N. da
700 1 $aSANTOS, R. S.
700 1 $aBALDANI, J. I.
700 1 $aURQUIAGA, S.
700 1 $aVIDAL, M. S.
700 1 $aARAUJO, J. L. S. de
773 $tPlant and Soil, 06 September 2021
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Esconder MarcMostrar Marc Completo |
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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| Registros recuperados : 11 | |
| 1. |  | BESERRA JÚNIOR, J. E. A.; NASCIMENTO, A. K. Q.; ANDRADE, E. C. de; LIMA, J. A. A. Análise molecular de isolados de Papaya lethal yellowingf virus (PLYV) obtidos no Ceará. Tropical Plant Pathology, Brasília, DF, v. 34, ago. 2009. Suplemento. Edição dos Resumos do XLII Congresso Brasileiro de Fitopatologia, Rio de Janeiro, ago. 2009. Suplemento. Resumo 939.| Tipo: Resumo em Anais de Congresso |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 3. | | SILVA, J. D. C.; BESERRA-JUNIOR, J. E. A.; GIRÃO-FILHO, J. E.; SILVA, R. B. Q.; MEDEIROS, W. R.; CARVALHO, D. S.; SILVA, P. R. R. First report of citrus blackfly (Hemiptera: Aleyrodidae) in the state of Piauí, Brazil. Brazilian Journal of Biology, v. 75, n. 2, p. 499-500, 2015.| Tipo: Nota Técnica/Nota Científica |
| Biblioteca(s): Embrapa Meio-Norte. |
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| 4. | | CHABI-JESUS, C.; RAMOS-GONZÁLEZ, P. L.; TASSI, A. D.; BARGUIL, B. M.; BESERRA JUNIOR, J. E. A.; HARAKAVA, R.; KITAJIMA, E. W.; ASTUA, J. de F. First Report of Citrus Chlorotic Spot Virus Infecting the Succulent Plant Agave desmettiana. Plant Disease, v.103, n. 6, June 2019.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 5. | | BESERRA JUNIOR, J. E. A.; ANDRADE, E. C. de; CAMARÇO, R. F. R. A.; NASCIMENTO, A. K. Q.; LIMA, J. A. A. Sequence variability in the coat protein gene of cowpea severe mosaic virus isolates from northeastern Brazil. Tropical Plant Pathology, Brasília, DF, v. 36, n. 2, p. 121-124, 2011. Rosa F. R. Araújo Camarço [i.e. Rosa Felícia Escóssia Araújo Camarço]| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 2 |
| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 6. | | SEVERO, R.; SHIBUTANI, L. J. S.; SILVA, G. F. da; GUIMARÃES, S. da S. C.; ALCÂNTARA NETO, F. de; BESERRA JUNIOR, J. E. A.; SOUZA, J. F. F. de; MELO, M. P. de. Fusarium species causing root rot and wilt in tomato in Brazil. Journal of Phytopathology, v. 172, n. 1, e13261, 2024.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 3 |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 7. | | MELO, M. P. de; CABRAL, C. S.; REIS, A.; MATOS, K. S.; MARTINS, P. P.; BESERRA JÚNIOR, J. E. A.; NECHET, K. de L.; HALFELD-VIEIRA, B. de A. Rhizoctonia solani AG 1-IB and AG 4 HG-I causing leaf blight and root rot in plants from the Lamiaceae family in Brazil. Tropical Plant Pathology, v. 43, n. 2, p. 152-159, Apr. 2018.| Tipo: Nota Técnica/Nota Científica |
| Biblioteca(s): Embrapa Hortaliças; Embrapa Meio Ambiente. |
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| 8. | | MEDEIROS, L. de S. A.; OLIVEIRA, I. A. de; KITAJIMA, E. W.; EIRAS, M.; PEREIRA, H. J.; RIBEIRO, S. G.; MATOS, K. da S.; BESERRA JÚNIOR, J. E. A. A survey of RNA genome viruses in Lima bean crops of Northeastern Brazil. Bragantia, v. 79, n. 3, p. 407-416, 2020.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: B - 1 |
| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 9. | | ROCHA, F. V. R. da; SEVERO, R.; SHIBUTANI, L. J. S.; VIEIRA, D. D. S. S.; MATOS, K. S.; GUIMARÃES, S. da S. C.; SILVA, G. F. da; FRANÇA, S. M. de; SOUSA, E. S.; BESERRA JUNIOR, J. E. A.; MELO, M. P. de. First report of Thielaviopsis ethacetica causing black rot in Bactris gasipaes fruit in Brazil. Plant Disease, v. 107, n. 11, p. 3634, 2023.| Tipo: Nota Técnica/Nota Científica |
| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| 10. | | MELO, M. P. de; MATOS, K. S.; MOREIRA, S. I.; SILVA, F. F.; CONCEIÇÃO, G. H.; NECHET, K. de L.; HALFELD-VIEIRA, B. de A.; BESERRA JUNIOR, J. E. A.; VENTURA, J. A.; COSTA, H.; FURTADO, E. L.; ALVES, E.; CERESINI, P. C. Two new Ceratobasidium species causing white thread blight on tropical plants in Brazil. Tropical Plant Pathology, v. 43, n. 6, p. 559-571, 2018.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 2 |
| Biblioteca(s): Embrapa Meio Ambiente. |
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| 11. | | XAVIER, C. A. D.; NOGUEIRA, A. M.; BELLO, V. H.; WATANABE, L. F. M.; BARBOSA, T. M. C.; ALVES JÚNIOR, M.; BARBOSA, L.; BESERRA-JÚNIOR, J. E. A.; BOARI, A. de J.; CALEGARIO, R.; GORAYEB, E. S.; HONORATO JÚNIOR, J.; KOCH, G.; LIMA, G. S. de A.; LOPES, C.; MELLO, R. N. de; PANTOJA, K.; SILVA, F. N.; RAMOS SOBRINHO, R.; SANTANA, E. N.; SILVA, J. W. P. da; KRAUSE-SAKATE, R.; ZERBINI, F. M. Assessing the diversity of whiteflies infesting cassava in Brazil. PeerJ, v. 9, e11741, July 2021.| Tipo: Artigo em Periódico Indexado | Circulação/Nível: A - 1 |
| Biblioteca(s): Embrapa Amazônia Oriental; Embrapa Arroz e Feijão. |
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| Registros recuperados : 11 | |
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| Nenhum registro encontrado para a expressão de busca informada. |
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