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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
06/06/1997 |
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Data da última atualização: |
06/08/2019 |
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Autoria: |
BARROS, L. M. G.; GAMA, M. I. C.; GONÇALVES, C. H. R. de P.; BARRETO, C. C.; SANTANA, E. F.; CARNEIRO, V. T. de C. |
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Afiliação: |
LEILA MARIA GOMES BARROS, Cenargen; MARIA I. CONCEIÇÃO S. GAMA, CENARGEN; CHRISTINA H. R. DE P. GONÇALVES, Multiplica Tecnologia Vegemi Ltda.; CRISTINE CHAVES BARRETO, CENARGEN; ELIANA F. SANTANA, CENARGEN; VERA TAVARES DE CAMPOS CARNEIRO, Cenargen. |
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Título: |
Cultura de tecidos de feijoeiro visando a introdução de genes exógenos. |
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Ano de publicação: |
1997 |
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Fonte/Imprenta: |
Pesquisa Agropecuária Brasileira, Brasília, DF, v. 32, n. 3, p. 267-275, mar. 1997. |
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Idioma: |
Português |
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Notas: |
Título em inglês: Bean tissue culture for introduction of foreign genes. |
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Conteúdo: |
O objetivo deste trabalho foi determinar parametros da cultura de tecidos de feijoeiro necessarios para a aplicacao da tecnica de transformacao via Agrobacterium. Diversos genotipos de feijoeiro (Phaseolus vulgaris L.) foram cultivados in vitro. Alguns parametros visando a infeccao de apices caulinares com Agrobacterium tumefaciens foram otimizados, como a eliminacao da agrobacteria apos a co-cultura e a concentracao de kanamicina suficiente para a selecao dos apices infectados. Multibrotacoes em nos cotiledonares foram obtidas com frequencia de ate 55,7% dependendo do genotipo utilizado. Estes nos foram infectados com A. tumefaciens contendo em seu plasmideo Ti o gene da B-glucuronidase (GUS) sob regulacao de promotor eucariotico. Entre as multibrotacoes obtidas 30% apresentaram regiao onde foi detectada atividade do gene GUS. Demonstrou-se tambem a susceptibilidade de feijoeiro a Agrobacterium rhizogenes. Raizes transgenicas foram obtidas apos infeccao de epicotilos. |
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Thesagro: |
Cultura In Vitro; Phaseolus Vulgaris. |
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Thesaurus Nal: |
Agrobacterium; in vitro culture. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/AI-SEDE/4833/1/pabmar05.pdf
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Marc: |
LEADER 01765naa a2200241 a 4500
001 1088283
005 2019-08-06
008 1997 bl uuuu u00u1 u #d
100 1 $aBARROS, L. M. G.
245 $aCultura de tecidos de feijoeiro visando a introdução de genes exógenos.
260 $c1997
500 $aTítulo em inglês: Bean tissue culture for introduction of foreign genes.
520 $aO objetivo deste trabalho foi determinar parametros da cultura de tecidos de feijoeiro necessarios para a aplicacao da tecnica de transformacao via Agrobacterium. Diversos genotipos de feijoeiro (Phaseolus vulgaris L.) foram cultivados in vitro. Alguns parametros visando a infeccao de apices caulinares com Agrobacterium tumefaciens foram otimizados, como a eliminacao da agrobacteria apos a co-cultura e a concentracao de kanamicina suficiente para a selecao dos apices infectados. Multibrotacoes em nos cotiledonares foram obtidas com frequencia de ate 55,7% dependendo do genotipo utilizado. Estes nos foram infectados com A. tumefaciens contendo em seu plasmideo Ti o gene da B-glucuronidase (GUS) sob regulacao de promotor eucariotico. Entre as multibrotacoes obtidas 30% apresentaram regiao onde foi detectada atividade do gene GUS. Demonstrou-se tambem a susceptibilidade de feijoeiro a Agrobacterium rhizogenes. Raizes transgenicas foram obtidas apos infeccao de epicotilos.
650 $aAgrobacterium
650 $ain vitro culture
650 $aCultura In Vitro
650 $aPhaseolus Vulgaris
700 1 $aGAMA, M. I. C.
700 1 $aGONÇALVES, C. H. R. de P.
700 1 $aBARRETO, C. C.
700 1 $aSANTANA, E. F.
700 1 $aCARNEIRO, V. T. de C.
773 $tPesquisa Agropecuária Brasileira, Brasília, DF$gv. 32, n. 3, p. 267-275, mar. 1997.
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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Registro Completo
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Biblioteca(s): |
Embrapa Uva e Vinho. |
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Data corrente: |
24/02/2011 |
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Data da última atualização: |
31/10/2019 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
GEBLER, L.; BERTOL, I.; RAMOS, R. R.; BARBOSA, F. T.; SCHRAMMEL, B. M.; LOUZADA, J. A. S. |
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Afiliação: |
LUCIANO GEBLER, CNPUV; ILDEGARDIS BERTOL, UDESC; ROGER ROBERT RAMOS, UDESC; FABRÍCIO TONDELLO BARBOSA, UDESC; BRUNA MOREIRA SCHRAMMEL, UCS; JOSÉ ANTÔNIO SALDANHA LOUZADA, UFRGS/IPH. |
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Título: |
Arraste superficial de fósforo reativo em pequenas bacias hidrográficas sob diferentes tipos de manejo agrícola e possível impacto nos recursos hídricos e no sistema de gestão: uma proposta preliminar. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: CONGRESO INTERAMERICANO DE INGENIERIA SANITARIA Y AMBIENTAL, 32.; FORO INTERAMERICANO SOBRE SERVICIOS DE AGUA Y SANEAMENTO, 2., 2010, Bávaro, República Dominicana. El cumplimento de las metas del milenio en la busqueda de la universalizacion de los servicios. [S.l.]: AIDIS, 2010. |
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Páginas: |
p. 1-7. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Português |
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Conteúdo: |
The behavior of dissolved reactive phosphorus in the soi! may depend on the different systems of soi! management, providing a timely management of impacts on the environment. The aim of this study was to assess lhe form of drag and reactive phosphorus in different types of ground cover offered and suggest alternatives to the traditional management in agricultural are as ofthe basins. The study was conducted in Lages, Se, between the November/2009 março/2010, a lnceptsoil under jive different types of crops randomly distributed, with two replications. There were three tests of simulated rain with 90 minutes in duration and intensity of 64 mm h-I, using rainfall simulator rotating boom type Swanson. The tests were carried out at 30, 69 and 105 days afier sowing. Losses of phosphorus in runoff varied with rainfall tests, but not with the types of farming system. The plots with soybean and corn were the bachelor results closer than expected, whi!e the plots of bare soi! and bean showed the highest difference of the expected behavior in relation to the behavior of reactive phosphorus in comparison to the total reactive phosphorus jiltrado. Com base this, it is suggested that management of small rural watersheds take into account the type of management that will be given to the ground in order to adapt where necessary physical structures to contain runof!. Likewise, the management should take into account the times that offer greater risk, which would be the time of planting and harvest, depending on the way it is made. KeyWords: Reactive Agricultural contaminants, agricultural environmental mamagement, risk indicator MenosThe behavior of dissolved reactive phosphorus in the soi! may depend on the different systems of soi! management, providing a timely management of impacts on the environment. The aim of this study was to assess lhe form of drag and reactive phosphorus in different types of ground cover offered and suggest alternatives to the traditional management in agricultural are as ofthe basins. The study was conducted in Lages, Se, between the November/2009 março/2010, a lnceptsoil under jive different types of crops randomly distributed, with two replications. There were three tests of simulated rain with 90 minutes in duration and intensity of 64 mm h-I, using rainfall simulator rotating boom type Swanson. The tests were carried out at 30, 69 and 105 days afier sowing. Losses of phosphorus in runoff varied with rainfall tests, but not with the types of farming system. The plots with soybean and corn were the bachelor results closer than expected, whi!e the plots of bare soi! and bean showed the highest difference of the expected behavior in relation to the behavior of reactive phosphorus in comparison to the total reactive phosphorus jiltrado. Com base this, it is suggested that management of small rural watersheds take into account the type of management that will be given to the ground in order to adapt where necessary physical structures to contain runof!. Likewise, the management should take into account the times that offer greater risk, which would be the time of planting and h... Mostrar Tudo |
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Palavras-Chave: |
Gestão ambiental; Indicador de risco. |
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Thesagro: |
Agricultura; Contaminação; Fósforo; Manejo; Meio ambiente; Recurso hídrico. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/204002/1/12969-2010.pdf
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Marc: |
LEADER 02783nam a2200277 a 4500
001 1878857
005 2019-10-31
008 2010 bl uuuu u00u1 u #d
100 1 $aGEBLER, L.
245 $aArraste superficial de fósforo reativo em pequenas bacias hidrográficas sob diferentes tipos de manejo agrícola e possível impacto nos recursos hídricos e no sistema de gestão$buma proposta preliminar.$h[electronic resource]
260 $aIn: CONGRESO INTERAMERICANO DE INGENIERIA SANITARIA Y AMBIENTAL, 32.; FORO INTERAMERICANO SOBRE SERVICIOS DE AGUA Y SANEAMENTO, 2., 2010, Bávaro, República Dominicana. El cumplimento de las metas del milenio en la busqueda de la universalizacion de los servicios. [S.l.]: AIDIS$c2010
300 $ap. 1-7.$c1 CD-ROM.
520 $aThe behavior of dissolved reactive phosphorus in the soi! may depend on the different systems of soi! management, providing a timely management of impacts on the environment. The aim of this study was to assess lhe form of drag and reactive phosphorus in different types of ground cover offered and suggest alternatives to the traditional management in agricultural are as ofthe basins. The study was conducted in Lages, Se, between the November/2009 março/2010, a lnceptsoil under jive different types of crops randomly distributed, with two replications. There were three tests of simulated rain with 90 minutes in duration and intensity of 64 mm h-I, using rainfall simulator rotating boom type Swanson. The tests were carried out at 30, 69 and 105 days afier sowing. Losses of phosphorus in runoff varied with rainfall tests, but not with the types of farming system. The plots with soybean and corn were the bachelor results closer than expected, whi!e the plots of bare soi! and bean showed the highest difference of the expected behavior in relation to the behavior of reactive phosphorus in comparison to the total reactive phosphorus jiltrado. Com base this, it is suggested that management of small rural watersheds take into account the type of management that will be given to the ground in order to adapt where necessary physical structures to contain runof!. Likewise, the management should take into account the times that offer greater risk, which would be the time of planting and harvest, depending on the way it is made. KeyWords: Reactive Agricultural contaminants, agricultural environmental mamagement, risk indicator
650 $aAgricultura
650 $aContaminação
650 $aFósforo
650 $aManejo
650 $aMeio ambiente
650 $aRecurso hídrico
653 $aGestão ambiental
653 $aIndicador de risco
700 1 $aBERTOL, I.
700 1 $aRAMOS, R. R.
700 1 $aBARBOSA, F. T.
700 1 $aSCHRAMMEL, B. M.
700 1 $aLOUZADA, J. A. S.
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