Data/hora: 24/06/2024 11:43:55

Biblioteca(s): | Embrapa Agrobiologia.
Data corrente: | 19/04/1999
Data da última atualização: | 19/04/1999
Autoria: | HUDDLESTON, A. S.; CRESSWELL, N.; NEVES, M. C. P.; BERINGER, J. E.; BAUMBERG, S.; THOMAS, D. I.; WELLINGTON, E. M. H.
Título: | Molecular detection of streptomycin-producing streptomycetes in Brazilian soils.
Ano de publicação: | 1997
Fonte/Imprenta: | Applied and Environmental Microbiology, Washington, v. 63, n. 4, p. 1288-1297, 1997.
Idioma: | Inglês
Conteúdo: | Actinomycetes were isolated from soybean rhizosphere soil collected at two field sites in Brazil. All the isolates were identified as Streptomyces species and were screened for streptomycin production and the presence of two genes, strA and strB1, known to be involved in streptomycin biosynthesis in Streptomyces griseus. Antibiotic resistance profiles were determined for 53 isolates from cultivated and uncultivated sites, and approximately half the strains wre streptomycin resistant. Clustering by the unweighted pair group method with averages indicated the presence of two major clusters, with the majority of resistant strains from cultivated sites being placed in cluster 1. Only representatives from this cluster contained strA. Streptomycetes containing   strA and strB1 were phenotypically diverse, and only half could be assigned to known species. Sequence comparison of 16S rRNA and trpBA (tryptophan synthetase) genes revealed that streptomycin-producing streptomycetes were phylogenetically diverse. It appeared that a population of streptomycetes had colonized the rhizosphere and that a proportion of these were capable of streptomycin production.
Palavras-Chave: | Brasil; Production; Resistance to chemicals.
Thesagro: | Actinomiceto; Antibiótico; Estreptomicina; Gene; Produção; Resistência a Produtos Químicos; Rizosfera; Soja; Solo.
Thesaurus Nal: | Actinomycetales; antibiotics; Brazil; genes; rhizosphere; soil; soybeans; Streptomyces griseus; streptomycin.
Categoria do assunto: | --
Registro original: | Embrapa Agrobiologia (CNPAB)
Exemplares: | CNPAB - (24416-1) - UPC - AP - -- -  -  - 22255 - DIS