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Registro Completo |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
30/08/2018 |
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Data da última atualização: |
30/08/2018 |
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Autoria: |
BOTELHO, A. F. M.; OLIVEIRA, F. A. S.; FIÚZA, A. T. L.; PEDROSA, H. P.; BRANCO, S. E. M. T.; PIEREZAN, F.; MELO, M. M.; SOTO-BLANCO, B. |
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Afiliação: |
Ana F. M. Botelho, Departamento de Clínica e Cirurgia Veterinárias/Escola de Veterinária/Universidade Federal de Minas Gerais - UFMG; Fabiano A. S. Oliveira, Laboratório Nacional Agropecuário - Lanagro/MG/Ministério da Agricultura, Pecuária e Abastecimento; Aparecida T. L. Fiúza, Departamento de Clínica e Cirurgia Veterinárias/Escola de Veterinária/Universidade Federal de Minas Gerais - UFMG; Heloísa P. Pedroza, Departamento de Clínica e Cirurgia Veterinárias/Escola de Veterinária/Universidade Federal de Minas Gerais - UFMG; Stephanie E. M. T. Branco, Departamento de Clínica e Cirurgia Veterinárias/Escola de Veterinária/Universidade Federal de Minas Gerais - UFMG; Felipe Pierezan, Departamento de Clínica e Cirurgia Veterinárias/Escola de Veterinária/Universidade Federal de Minas Gerais - UFMG; Marília M. Melo, Departamento de Clínica e Cirurgia Veterinárias/Escola de Veterinária/Universidade Federal de Minas Gerais - UFMG; Benito Soto-Blanco, Departamento de Clínica e Cirurgia Veterinárias/Escola de Veterinária/Universidade Federal de Minas Gerais - UFMG. |
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Título: |
Improved method for diagnosis of Nerium oleander poisoning in necropsy tissues. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 5, p. 967-972, maio 2018 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Método aprimorado para diagnóstico da intoxicação por Nerium oleander em tecidos de necropsia. |
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Conteúdo: |
Nerium oleander is an ornamental cardiotoxic plant found in tropical and subtropical areas of the World. Its toxicity is related to the content of cardioactive glycosides, mainly oleandrin, found throughout the plant. The present study aimed to describe a new and improved method for oleandrin detection in tissue samples. The determination of oleandrin was made after extraction with a modified QuEChERS technique and measurement by UFLC-MS/MS. A total of 36 guinea pigs (Cavia porcellus) were distributed into 3 groups (n=12): control group that received only water orally (CON), and two treated groups that received hydroalcoholic oleander extract at doses of 150mg.kg-1 (OLE 150) and 300mg.kg-1 (OLE 300) in single oral dose. After three hours, fragments of heart, kidneys, liver and brain were collected for determination of oleandrin levels. The extraction and chromatographic procedures were effective for oleandrin detection and quantification in tissues, with retention time of 1.2 min and detection limit of 0.001μg g-1. The chromatographic analysis of treated guinea pigs indicated that oleandrin is distributed equally among the analyzed tissues. The developed methodology is a reliable, effective and rapid form of diagnosis of N. oleander poisoning based on necropsy tissue samples. |
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Palavras-Chave: |
Cromatografia líquida; Espectrometria de massa; Teste diagnóstico; Toxicose. |
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Thesagro: |
Cobaia; Oleander. |
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Thesaurus Nal: |
Cardenolides; Guinea pigs; Liquid chromatography; Mass spectrometry; Nerium oleander. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/182115/1/Improved-method-for-diagnosis-of-nerium-oleander.pdf
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Marc: |
LEADER 02425naa a2200349 a 4500
001 2094762
005 2018-08-30
008 2018 bl uuuu u00u1 u #d
100 1 $aBOTELHO, A. F. M.
245 $aImproved method for diagnosis of Nerium oleander poisoning in necropsy tissues.$h[electronic resource]
260 $c2018
500 $aTítulo em português: Método aprimorado para diagnóstico da intoxicação por Nerium oleander em tecidos de necropsia.
520 $aNerium oleander is an ornamental cardiotoxic plant found in tropical and subtropical areas of the World. Its toxicity is related to the content of cardioactive glycosides, mainly oleandrin, found throughout the plant. The present study aimed to describe a new and improved method for oleandrin detection in tissue samples. The determination of oleandrin was made after extraction with a modified QuEChERS technique and measurement by UFLC-MS/MS. A total of 36 guinea pigs (Cavia porcellus) were distributed into 3 groups (n=12): control group that received only water orally (CON), and two treated groups that received hydroalcoholic oleander extract at doses of 150mg.kg-1 (OLE 150) and 300mg.kg-1 (OLE 300) in single oral dose. After three hours, fragments of heart, kidneys, liver and brain were collected for determination of oleandrin levels. The extraction and chromatographic procedures were effective for oleandrin detection and quantification in tissues, with retention time of 1.2 min and detection limit of 0.001μg g-1. The chromatographic analysis of treated guinea pigs indicated that oleandrin is distributed equally among the analyzed tissues. The developed methodology is a reliable, effective and rapid form of diagnosis of N. oleander poisoning based on necropsy tissue samples.
650 $aCardenolides
650 $aGuinea pigs
650 $aLiquid chromatography
650 $aMass spectrometry
650 $aNerium oleander
650 $aCobaia
650 $aOleander
653 $aCromatografia líquida
653 $aEspectrometria de massa
653 $aTeste diagnóstico
653 $aToxicose
700 1 $aOLIVEIRA, F. A. S.
700 1 $aFIÚZA, A. T. L.
700 1 $aPEDROSA, H. P.
700 1 $aBRANCO, S. E. M. T.
700 1 $aPIEREZAN, F.
700 1 $aMELO, M. M.
700 1 $aSOTO-BLANCO, B.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 38, n. 5, p. 967-972, maio 2018
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Registro original: |
Embrapa Unidades Centrais (AI-SEDE) |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
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URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
06/12/2021 |
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Data da última atualização: |
04/10/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 5 |
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Autoria: |
BENTO, J. A. C.; MORAIS, D. K.; BERSE, R. S. de; BASSINELLO, P. Z.; CALIARI, M.; SOARES JÚNIOR, M. S. |
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Afiliação: |
JULIANA APARECIDA CORREIA BENTO, UFG; DAISY KARIONE MORAIS, UFG; RAFAEL SIQUEIRA DE BERSE, UFG; PRISCILA ZACZUK BASSINELLO, CNPAF; MÁRCIO CALIARI, UFG; MANOEL SOARES SOARES JÚNIOR, UFG. |
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Título: |
Functional, thermal, and pasting properties of cooked carioca bean (Phaseolus vulgaris L.) flours. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Applied Food Research, v. 2, n. 1, 100027, June 2022. |
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ISSN: |
2772-5022 |
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DOI: |
https://doi.org/10.1016/j.afres.2021.100027 |
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Idioma: |
Inglês |
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Conteúdo: |
This study verified if cooking presoaked beans in the steam of autoclave improves the pasting properties, texture profile, water-solubility (WSI), emulsifying capacities of aged carioca bean' flours. The carioca beans flour presented high content of protein (20.7 -22.3 g.100g -1), resistant starch (RS) (8.3 - 31.1 g.100g -1), and dietary fiber (TDF) (18.9 - 23.7 g. 100g -1), and the cultivar Notavel presented the highest content of total dietary fiber and resistant starch for both cooked and raw flour. The pretreatment promoted an increase in TDF (8.8 %, cultivar Dama) and a decrease in RS (19.5 %, 33.4 %, and 47.0 % for cultivars Imperador, Gol, and Bola Cheia, respectively). Regarding the pasting properties, the heating process promoted a reduction in the values of peak viscosity, final viscosity, breakdown, and setback for all carioca bean cultivars. The other parameters, i.e., gel hardness, WSI, emulsifying capacity, and stability also presented a significant decrease in the cooked flours. So, the pretreatment promoted a total or/partially starch pre-gelatinization and the denaturation of the proteins of the flours which might increase their acceptability for food development. |
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Palavras-Chave: |
Paste viscosity. |
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Thesagro: |
Farinha; Feijão; Phaseolus Vulgaris; Viscosidade. |
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Thesaurus NAL: |
Bean flour; Beans; Emulsifying properties; Hardness; Resistant starch; Viscosity; Water solubility. |
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Categoria do assunto: |
Q Alimentos e Nutrição Humana |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1137183/1/afr-2022.pdf
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Marc: |
LEADER 02200naa a2200349 a 4500
001 2137183
005 2022-10-04
008 2022 bl uuuu u00u1 u #d
022 $a2772-5022
024 7 $ahttps://doi.org/10.1016/j.afres.2021.100027$2DOI
100 1 $aBENTO, J. A. C.
245 $aFunctional, thermal, and pasting properties of cooked carioca bean (Phaseolus vulgaris L.) flours.$h[electronic resource]
260 $c2022
520 $aThis study verified if cooking presoaked beans in the steam of autoclave improves the pasting properties, texture profile, water-solubility (WSI), emulsifying capacities of aged carioca bean' flours. The carioca beans flour presented high content of protein (20.7 -22.3 g.100g -1), resistant starch (RS) (8.3 - 31.1 g.100g -1), and dietary fiber (TDF) (18.9 - 23.7 g. 100g -1), and the cultivar Notavel presented the highest content of total dietary fiber and resistant starch for both cooked and raw flour. The pretreatment promoted an increase in TDF (8.8 %, cultivar Dama) and a decrease in RS (19.5 %, 33.4 %, and 47.0 % for cultivars Imperador, Gol, and Bola Cheia, respectively). Regarding the pasting properties, the heating process promoted a reduction in the values of peak viscosity, final viscosity, breakdown, and setback for all carioca bean cultivars. The other parameters, i.e., gel hardness, WSI, emulsifying capacity, and stability also presented a significant decrease in the cooked flours. So, the pretreatment promoted a total or/partially starch pre-gelatinization and the denaturation of the proteins of the flours which might increase their acceptability for food development.
650 $aBean flour
650 $aBeans
650 $aEmulsifying properties
650 $aHardness
650 $aResistant starch
650 $aViscosity
650 $aWater solubility
650 $aFarinha
650 $aFeijão
650 $aPhaseolus Vulgaris
650 $aViscosidade
653 $aPaste viscosity
700 1 $aMORAIS, D. K.
700 1 $aBERSE, R. S. de
700 1 $aBASSINELLO, P. Z.
700 1 $aCALIARI, M.
700 1 $aSOARES JÚNIOR, M. S.
773 $tApplied Food Research$gv. 2, n. 1, 100027, June 2022.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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