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| Registros recuperados : 133 | |
| 61. |  | BENAVIDES, M. V.; WEIMER, T. A.; BORBA, M. F. S.; BERNE, M. E. A.; SACCO, A. M. S. Genetic analyses of polymorphisms on ovine chromosomes 5 and 20 and their effect on resistance to internal parasites. Small Ruminant Research, Amsterdam, v. 83, n.1-3, p. 67-73, May 2009.| Biblioteca(s): Embrapa Pecuária Sul. |
|    |
| 64. | | BENAVIDES, M. V.; ECHEVARRIA, F. A. M.; SONSTEGARD, T. S.; VAN TESSEL, C. P.; GASBARRE, L. C. Genetic variability of a Bos taurus x Bos indicus cross population and validation of genomic regions influencing nematode resistance. In: SIMPOSIO DE RECURSOS GENETICOS PARA AMERICA LATINA Y EL CARIBE, 5., 2005, Montevideo, Uruguay. Resumenes... Montevideo: INIA :Facultad de Agronomía de la Universidad de la República, 2005. p. 105 SIRGEALC. Resumo 305.| Biblioteca(s): Embrapa Pecuária Sul. |
|  |
| 69. | | CHAGAS, E. C.; PEREIRA, S. L. A.; BENAVIDES, M. V.; BRANDÃO, F. R.; MONTEIRO, P. C.; MACIEL, P. O. Neoechinorhynchus buttnerae parasitic infection in tambaqui (Colossoma macropomum) on fish farms in the state of Amazonas. Boletim do Instituto de Pesca, v. 45, n. 2, e499, 2019.| Biblioteca(s): Embrapa Amazônia Ocidental; Embrapa Pecuária Sul; Embrapa Pesca e Aquicultura; Embrapa Roraima. |
| |
| 71. | | BENAVIDES, M. V.; SOUZA, C. J. H. de; MORAES, J. C. F.; HASSUM, I. C.; BERNE, M. E. A. Dinâmica da contaminação de larvas infectantes de parasitos gastrintestinais em potreiros experimentais. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA, 21.; ENCONTRO DE PARASITOLOGIA DO MERCOSUL, 2., 2009, Foz do Iguaçu. Novos horizontes em parasitologia: anais. Porto Alegre: Sociedade Brasileira de Parasitologia; Curitiba: UFPR, 2009. 1 CD-ROM.| Biblioteca(s): Embrapa Pecuária Sul. |
| |
| 74. | | SOUZA, C. J. H. de; McNEILLY, A. S.; BENAVIDES, M. V.; MELO, E. O.; MORAES, J. C. F. Mutation in the protease cleavage site of GDF9 increases ovulation rate and litter size in heterozygous ewes and causes infertility in homozygous ewes. Animal Genetics, Oxford, v. 45, n. 5, p. 732-739, Oct. 2014.| Biblioteca(s): Embrapa Pecuária Sul; Embrapa Pesca e Aquicultura; Embrapa Recursos Genéticos e Biotecnologia. |
| |
| 78. | | BENAVIDES, M. V.; MILANO, L. R.; CORREA, A. P. R.; ALMEIDA, L. S.; FRANCK, B. M.; SACCO, A. M. S. Variabilidade genética do fator de necrose tumoral alfa (TNFa) em Bos taurus desafiados com cepa virulenta de Babesia bovis. In: CONGRESSO BRASILEIRO DE GENÉTICA, 51., 2005, Águas de Lindóia, SP. A era da genômica: da bioestatística à bioinformática: anais. Ribeirão Preto, SP: Sociedade Brasileira de Genética, 2005. p. 48.| Biblioteca(s): Embrapa Pecuária Sul. |
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| 80. | | NICIURA, S. C. M.; CRUVINEL, G. G.; MORAES, C. V.; DONATONI, F. A. B.; MALAGO JUNIOR, W.; BENAVIDES, M. V.; CHAGAS, A. C. de S. PCR-based genotyping of SNP markers in sheep. Molecular Biology Reports, v.45, n.4, p.651-654, aug. 2018.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| Registros recuperados : 133 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Pecuária Sudeste; Embrapa Pecuária Sul. |
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Data corrente: |
16/11/2022 |
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Data da última atualização: |
16/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
NICIURA, S. C. M.; OKINO, C. H.; NUCCI, A. DA S.; MALAGO JUNIOR, W.; BENAVIDES, M. V.; ESTEVES, S. N.; CHAGAS, A. C. de S. |
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Afiliação: |
SIMONE CRISTINA MEO NICIURA, CPPSE; CINTIA HIROMI OKINO, CPPSE; ALESSANDRA DA SILVA NUCCI, Centro Universitário Central Paulista; WILSON MALAGO JUNIOR, CPPSE; MAGDA VIEIRA BENAVIDES, CPPSUL; SERGIO NOVITA ESTEVES, CPPSE; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
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Título: |
Polymorphisms in exon 11 of the mptl-1 gene and monepantel resistance in Haemonchus contortus. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Parasitology Research, v. 121, p. 3581-3588, 2022. |
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DOI: |
https://doi.org/10.1007/s00436-022-07682-6 |
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Idioma: |
Inglês |
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Conteúdo: |
Chemical treatments are the main strategy to control gastrointestinal nematodes in sheep, and the emergence of anthelmintic resistance, as consequence, results in control failures and leads to economic losses. Thus, molecular tests may constitute an excellent tool for the early detection of anthelmintic resistance-related mutations. Thus, a polymerase chain reaction (PCR)-based genotyping assay followed by polyacrylamide gel electrophoresis (PAGE) was developed to detect polymorphisms in exon 11 of the acetylcholine receptor monepantel-1 gene (mptl-1) that were previously associated with monepantel resistance through a genome-wide study in Haemonchus contortus. DNA samples recovered from individual and pooled third-stage larvae from two susceptible feld-derived isolates and fve (three in vivo-derived and two feld-derived) resistant populations were used. New polymorphisms, including a 6-bp deletion and a 3-bp insertion, were detected in resistant individuals. These indels, confrmed using sequencing of cloned PCR products, are predicted to result in amino acid changes in transmembrane domain 2 (TMD2) of the MPTL-1 protein. The two susceptible isolates showed only the presence of the wild-type allele (100%), whereas lower frequencies of the wild-type allele were detected in monepantel-resistant populations (11.1 to 66.7%). These fndings report new polymorphisms in the mptl-1 gene, validate the results obtained through genomic mapping for monepantel resistance, and provide a PCR-based assay to genotype indels located in exon 11 of mptl-1 in H. contortus. MenosChemical treatments are the main strategy to control gastrointestinal nematodes in sheep, and the emergence of anthelmintic resistance, as consequence, results in control failures and leads to economic losses. Thus, molecular tests may constitute an excellent tool for the early detection of anthelmintic resistance-related mutations. Thus, a polymerase chain reaction (PCR)-based genotyping assay followed by polyacrylamide gel electrophoresis (PAGE) was developed to detect polymorphisms in exon 11 of the acetylcholine receptor monepantel-1 gene (mptl-1) that were previously associated with monepantel resistance through a genome-wide study in Haemonchus contortus. DNA samples recovered from individual and pooled third-stage larvae from two susceptible feld-derived isolates and fve (three in vivo-derived and two feld-derived) resistant populations were used. New polymorphisms, including a 6-bp deletion and a 3-bp insertion, were detected in resistant individuals. These indels, confrmed using sequencing of cloned PCR products, are predicted to result in amino acid changes in transmembrane domain 2 (TMD2) of the MPTL-1 protein. The two susceptible isolates showed only the presence of the wild-type allele (100%), whereas lower frequencies of the wild-type allele were detected in monepantel-resistant populations (11.1 to 66.7%). These fndings report new polymorphisms in the mptl-1 gene, validate the results obtained through genomic mapping for monepantel resistance, and provide a PC... Mostrar Tudo |
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Palavras-Chave: |
Amino acetonitrile derivatives; Anthelmintic resistance; PCR. |
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Thesagro: |
Haemonchus Contortus. |
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Thesaurus NAL: |
Genotyping. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02402naa a2200265 a 4500
001 2148276
005 2022-11-16
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s00436-022-07682-6$2DOI
100 1 $aNICIURA, S. C. M.
245 $aPolymorphisms in exon 11 of the mptl-1 gene and monepantel resistance in Haemonchus contortus.$h[electronic resource]
260 $c2022
520 $aChemical treatments are the main strategy to control gastrointestinal nematodes in sheep, and the emergence of anthelmintic resistance, as consequence, results in control failures and leads to economic losses. Thus, molecular tests may constitute an excellent tool for the early detection of anthelmintic resistance-related mutations. Thus, a polymerase chain reaction (PCR)-based genotyping assay followed by polyacrylamide gel electrophoresis (PAGE) was developed to detect polymorphisms in exon 11 of the acetylcholine receptor monepantel-1 gene (mptl-1) that were previously associated with monepantel resistance through a genome-wide study in Haemonchus contortus. DNA samples recovered from individual and pooled third-stage larvae from two susceptible feld-derived isolates and fve (three in vivo-derived and two feld-derived) resistant populations were used. New polymorphisms, including a 6-bp deletion and a 3-bp insertion, were detected in resistant individuals. These indels, confrmed using sequencing of cloned PCR products, are predicted to result in amino acid changes in transmembrane domain 2 (TMD2) of the MPTL-1 protein. The two susceptible isolates showed only the presence of the wild-type allele (100%), whereas lower frequencies of the wild-type allele were detected in monepantel-resistant populations (11.1 to 66.7%). These fndings report new polymorphisms in the mptl-1 gene, validate the results obtained through genomic mapping for monepantel resistance, and provide a PCR-based assay to genotype indels located in exon 11 of mptl-1 in H. contortus.
650 $aGenotyping
650 $aHaemonchus Contortus
653 $aAmino acetonitrile derivatives
653 $aAnthelmintic resistance
653 $aPCR
700 1 $aOKINO, C. H.
700 1 $aNUCCI, A. DA S.
700 1 $aMALAGO JUNIOR, W.
700 1 $aBENAVIDES, M. V.
700 1 $aESTEVES, S. N.
700 1 $aCHAGAS, A. C. de S.
773 $tParasitology Research$gv. 121, p. 3581-3588, 2022.
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