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Registro Completo |
Biblioteca(s): |
Embrapa Clima Temperado. |
Data corrente: |
07/01/2015 |
Data da última atualização: |
07/01/2015 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
TERRA, V. S. S.; VALGAS, R. A.; REISSER JUNIOR, C.; TIMM, L. C.; PEREIRA, J. F. M.; CARVALHO, F. L. C. |
Afiliação: |
Viviane Santos Silva Terra, UFPEL; RICARDO ALEXANDRE VALGAS, CPACT; CARLOS REISSER JUNIOR, CPACT; Luis Carlos Timm, UFPEL; JOSE FRANCISCO MARTINS PEREIRA, CPACT; FLAVIO LUIZ CARPENA CARVALHO, CPACT. |
Título: |
Multivariate analysis applied to the study of the relationship between soil and plant properties In a peach orchard(1). |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Revista Brasileira de Ciência do Solo, v. 38, p. 755-764, 2014. |
Idioma: |
Português |
Palavras-Chave: |
Canonical correlation. |
Thesagro: |
Prunus Persica. |
Thesaurus Nal: |
soil physical properties. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/115005/1/Flavio-Carvalho-07.pdf
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Marc: |
LEADER 00687naa a2200205 a 4500 001 2004606 005 2015-01-07 008 2014 bl uuuu u00u1 u #d 100 1 $aTERRA, V. S. S. 245 $aMultivariate analysis applied to the study of the relationship between soil and plant properties In a peach orchard(1).$h[electronic resource] 260 $c2014 650 $asoil physical properties 650 $aPrunus Persica 653 $aCanonical correlation 700 1 $aVALGAS, R. A. 700 1 $aREISSER JUNIOR, C. 700 1 $aTIMM, L. C. 700 1 $aPEREIRA, J. F. M. 700 1 $aCARVALHO, F. L. C. 773 $tRevista Brasileira de Ciência do Solo$gv. 38, p. 755-764, 2014.
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Embrapa Clima Temperado (CPACT) |
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![](/consulta/web/img/deny.png) | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sul. Para informações adicionais entre em contato com cppsul.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Pecuária Sul. |
Data corrente: |
23/01/2019 |
Data da última atualização: |
23/01/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
NICIURA, S. C. M.; CRUVINEL, G. G.; MORAES, C. V.; DONATONI, F. A. B.; MALAGO JUNIOR, W.; BENAVIDES, M. V.; CHAGAS, A. C. de S. |
Afiliação: |
SIMONE CRISTINA MEO NICIURA, CPPSE; Giovanna Gabrielle Cruvinel, UNICEP; Caroline Valério Moraes, UFSCar; FLAVIA ALINE BRESSANI DONATONI, CPPSE; WILSON MALAGO JUNIOR, CPPSE; MAGDA VIEIRA BENAVIDES, CPPSUL; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
Título: |
PCR-based genotyping of SNP markers in sheep. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Molecular Biology Reports, v. 45, n. 4, p. 651-654, Aug. 2018. |
DOI: |
https://doi.org/10.1007/s11033-018-4206-8 |
Idioma: |
Inglês |
Conteúdo: |
Single nucleotide polymorphisms (SNPs) are the main type of variation in genome, enabling them to be associated with traits of economic importance in livestock. Genome-wide association studies (GWAS) have led to the discovery of SNPs associated with desirable traits in sheep. However, in these studies, SNPs are genotyped by high-throughput methods in genome scale, which are expensive and require sophisticated equipment and analysis methods. Therefore, the goal of this study was to develop a reliable, rapid, and inexpensive polymerase chain reaction (PCR)-based method to genotype a medium number of animals for a few candidate SNPs previously associated with desirable phenotypes in sheep by GWAS, using markers associated with gastrointestinal nematode resistance as a model. DNA extracted from white-blood cells of 150 sheep was submitted to PCR amplification followed by agarose gel electrophoresis and determination of banding pattern. Tetra-primer ARMS-PCR was successfully optimized after changes in annealing temperature; annealing and extension times; concentration of MgCl2 and DNA; ratios of inner, outer, forward and reverse primer; and addition of adjuvants, for genotyping the OAR2_14765360, OAR6_81718546, OAR11_62887032, and OAR12_69606944 SNPs in sheep. An extensive optimization of tetra-primer ARMS-PCR resulted in a suitable, simple, cost-effective PCR-based method of genotyping four SNP markers previously detected by chip arrays. |
Palavras-Chave: |
PCR RFLP; Resistência nematódeo gastrintestinal; Tetra primer ARMS PCR. |
Thesagro: |
Marcador Molecular; Ovino. |
Thesaurus NAL: |
Genome. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 02277naa a2200277 a 4500 001 2104694 005 2019-01-23 008 2018 bl --- 0-- u #d 024 7 $ahttps://doi.org/10.1007/s11033-018-4206-8$2DOI 100 1 $aNICIURA, S. C. M. 245 $aPCR-based genotyping of SNP markers in sheep.$h[electronic resource] 260 $c2018 520 $aSingle nucleotide polymorphisms (SNPs) are the main type of variation in genome, enabling them to be associated with traits of economic importance in livestock. Genome-wide association studies (GWAS) have led to the discovery of SNPs associated with desirable traits in sheep. However, in these studies, SNPs are genotyped by high-throughput methods in genome scale, which are expensive and require sophisticated equipment and analysis methods. Therefore, the goal of this study was to develop a reliable, rapid, and inexpensive polymerase chain reaction (PCR)-based method to genotype a medium number of animals for a few candidate SNPs previously associated with desirable phenotypes in sheep by GWAS, using markers associated with gastrointestinal nematode resistance as a model. DNA extracted from white-blood cells of 150 sheep was submitted to PCR amplification followed by agarose gel electrophoresis and determination of banding pattern. Tetra-primer ARMS-PCR was successfully optimized after changes in annealing temperature; annealing and extension times; concentration of MgCl2 and DNA; ratios of inner, outer, forward and reverse primer; and addition of adjuvants, for genotyping the OAR2_14765360, OAR6_81718546, OAR11_62887032, and OAR12_69606944 SNPs in sheep. An extensive optimization of tetra-primer ARMS-PCR resulted in a suitable, simple, cost-effective PCR-based method of genotyping four SNP markers previously detected by chip arrays. 650 $aGenome 650 $aMarcador Molecular 650 $aOvino 653 $aPCR RFLP 653 $aResistência nematódeo gastrintestinal 653 $aTetra primer ARMS PCR 700 1 $aCRUVINEL, G. G. 700 1 $aMORAES, C. V. 700 1 $aDONATONI, F. A. B. 700 1 $aMALAGO JUNIOR, W. 700 1 $aBENAVIDES, M. V. 700 1 $aCHAGAS, A. C. de S. 773 $tMolecular Biology Reports$gv. 45, n. 4, p. 651-654, Aug. 2018.
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