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| Registros recuperados : 106 | |
| 81. |  | OLIVEIRA, A. S.; MIGLIOLO, L.; AQUINO, R. O.; RIBEIRO, J. K. C.; MACEDO, L. L. P.; COELHO, F. S.; SANCHES, A. P. C.; BEMQUERER, M. P.; SANTOS, E. A.; KIYOTA, S. SALES, M. P. Purification of a kunitz-type inhibitor from Pithecolobium dumosum seeds and its effect towards insects. In: ANNUAL MEETING OF THE SBBq, 36.; IUBMB CONFERENCE, 10., 2007, Salvador, BA. Infectious diseases: biochemistry of parasites, vectors and hosts: program and abstracts. São Paulo, SP: Brazilian Society for Biochemistry and Molecular Biology, 2007.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 82. | | MOREIRA, J. S.; OLIVEIRA, J. S.; BEMQUERER, M. P.; MACHADO-DE-ÁVILA, R. A.; SANTOS, D. M.; MATOS, D. C.; MARIA, B. T.; MAGALHÃES, P. P.; FARIAS, L. M. Purification of multifunctional substances active against Shigella sonnei. International Journal of Biological and Natural Sciences, v. 2, n. 5, p. 1-15, 2022.| Biblioteca(s): Embrapa Gado de Leite. |
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| 83. | | PAULA, D. P.; CLAUDINO, D.; TIMBÓ, R. V.; MIRANDA, J. E.; BEMQUERER, M. P.; RIBEIRO, A. C. J.; SUJII, E. R.; FONTES, E. M. G.; PIRES, C. S. S. Reproductive dormancy in boll-weevil from populations of the Midwest of Brazil. Journal of Economic Entomology, v. 106, n. 1, p. 86-96. 2013.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 84. | | OLIVEIRA, A. S.; AQUINO, R. O.; MIGLIOLO, L.; RIBEIRO, J. K. C.; MACEDO, L. L. P.; COELHO, F. S.; FOOK, J. M. L. L.; BEMQUERER, M. P.; SANTOS, E. A.; KIYOTA, S.; SALES, M. P. Two isoforms of kunitz-type inhibitors from Pithecolobium dumosum and their in vitro activities against insect larvae. In: ANNUAL MEETING OF THE SBBq, 36.; IUBMB CONFERENCE, 10., 2007, Salvador, BA. Infectious diseases: biochemistry of parasites, vectors and hosts: program and abstracts. São Paulo, SP: Brazilian Society for Biochemistry and Molecular Biology, 2007.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 85. | | VERLY, R. M.; RESENDE, J. M.; COELHO JUNIOR, E. F.; MAGALHÃES, M. T. Q. de; GUIMARÃES, C. F. C. R.; MUNHOZ, V. H. O.; BEMQUERER, M. P.; ALMEIDA, F. C. L.; SANTORO, M. M.; PILÓ-VELOSO, D.; BECHINGER, B. Structure and membrane interactions of the homodimeric antibiotic peptide homotarsinin. Scientific Reports, v. 7, 2017. Article 40854.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 86. | | HASSEMER, M. J.; BLASSIOLI-MORAES, M. C.; BORGES, M.; LAUMANN, R. A.; BEMQUERER, M.; RODRIGUES, M.; COSTA, E. S.; VAZ JUNIOR, S.; MAGALHÃES, D. M.; MICHEREFF, M. F. F. O uso de nanopartículas para a liberação controlada de semioquímicos de insetos e plantas. In: WORKSHOP DE NANOTECNOLOGIA APLICADA AO AGRONEGÓCIO, 9., 2017, São Carlos, SP. Anais ... São Carlos: Embrapa Instrumentação, 2017. p. 466-470. Editores: Caue Ribeiro; Elaine Cristina Paris; Luiz Henrique Capparelli Mattoso; Marcelo Porto Bemquerer; Maria Alice Martins; Odílio Benedito Garrido De Assis.| Biblioteca(s): Embrapa Agroenergia; Embrapa Recursos Genéticos e Biotecnologia. |
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| 87. | | MAGARELLI, G.; SILVA, J. G. da; RIBEIRO, C. L.; FREITAS, T. V. de; RODRIGUES, M. A.; GIL, E. de S.; MARRACCINI, P.; SOUZA, J. R. de; CASTRO, C. S. P. de; BEMQUERER, M. P. A voltammetric peptide biosensor for Cu2+ metal ion quantification in coffee seeds. Journal of Inorganic Biochemistry, v. 251, 112441, 2024.| Biblioteca(s): Embrapa Gado de Leite; Embrapa Recursos Genéticos e Biotecnologia; Embrapa Unidades Centrais. |
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| 88. | | MACHADO, R. J. A.; MONTEIRO, N. K. V.; SERQUIZ, P. R.; CARVALHO, F. M. C.; BEZERRA, A. D. L.; MIGLIOLO, L.; SILVA, O. N.; PINTO, M. S.; OLIVEIRA, A. S.; FRANCO, O. L.; KIYOTA, S.; BEMQUERER, M. P.; MORAIS, A. H. A.; SANTOS, E. A. Anticoagulant and anti-inflammatory activities of a trypsin inhibitor present in Erythrina velutina seeds. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE BIOQUÍMICS E BIOLOGIA MOLECULAR - SBBq, 41., Foz do Iguaçú, Brasil. Resumos... Foz do Iguaçu: SBBq, 2012.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 89. | | CARVALHO, K. G. de; BAMBIRRA, F. H. S.; KRUGER, M. F; BARBOSA, M. S.; OLIVEIRA, J. S.; SANTOS, A. M. C.; NICOLI, J. R.; BEMQUERER, M. P.; MIRANDA, A. de; SALVUCCI, E. J.; SESMA, F. J. M.; FRANCO, B. D. G. M. Antimicrobial compounds produced by Lactobacillus sakei subsp. sakei 2a, a bacteriocinogenic strain isolated from a Brazilian meat product. Journal of Industrial Microbiology and Biotechnology , v. 37, p. 381-390, 2010.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 90. | | SILVA, L. P. da; BEMQUERER, M. P.; RECH FILHO, E. L.; BLOCH JUNIOR, C.; RODRIGUES, M. A.; TELES, R. C. L.; BARBOSA, E. F.; DOMINGUES, G. S.; MEDEIROS, K. A.; FIARAVANTE, T. F.; CURLEY, R. C.; SOUZA, A. A. A. DE; NOBREGA, M. M.; OLIVEIRA, K. M.; MOITA, S. R.; OLIVEIRA, G. G. Aplicações nanobiotecnológicas para subprodutos das cadeias produtivas animal e vegetal. In: SIMPÓSIO SOBRE INOVAÇÃO E CRIATIVIDADE CIENTÍFICA NA EMBRAPA, 2., 2010, Brasília, DF. Anais... Brasília, DF: Embrapa, 2010. Apresentado também no ENCONTRO DO INSTITUTO NACIONAL DE CIÊNCIA E TECNOLOGIA EM NANOBIOTECNOLOGIA, 1., 2010, Brasília. [Anais...]. Brasília: UnB, 2010.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 91. | | OLIVEIRA, K. C. B. F.; FARIAS, E. A. de O.; TEIXEIRA, P. R. S.; BRANDÃO, V. S.; SÁBIO, R. M.; ARAÚJO, A. R. de; EATON, P.; BERTOLINO, L. C.; BEMQUERER, M. P.; BARUD, H. da S.; LEITE, J. R. de S. de A.; EIRAS, C. Development of a nanostructured film containing palygorskite and dermaseptin 01 peptide for biotechnological applications. Clays and Clay Minerals, v. 71, p. 600-615, 2023.| Biblioteca(s): Embrapa Gado de Leite. |
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| 92. | | SOARES, T. G.; SANTOS, J. L. dos; ALVARENGA, V. G. de; SANTOS, J. S. C.; LECLERCQ, S. Y.; FARIA, C. D.; OLIVEIRA, M. A. A.; BEMQUERER, M. P.; SANCHEZ, E. O. F.; LIMA, M. E. de; FIGUEIREDO, S. G.; BORGES, M. H. Biochemical and functional properties of a new L-amino acid oxidase (LAAO) from Micrurus lemniscatus snake venom. International Journal of Biological Macromolecules, v.154, p. 1517-1527, 2020.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 93. | | TORRES, L. M. F. C.; BRAGA, N. A.; GOMES, I. P.; ALMEIDA, M. T.; SANTOS, T. L.; MESQUITA, J. P. de; SILVA, L. M. da; MARTINS, H. R.; KATO, K. C.; SANTOS, W. T. P. dos; RESENDE, J. M.; PEREIRA, M. C.; BEMQUERER, M. P.; RODRIGUES, M. A.; VERLY, R. M. Nanobiostructure of fibrous-like alumina functionalized with analog of the BP100 peptide: synthesis, characterization and biological applications. Colloids and Surfaces B: Biointerfaces, v. 163, p. 275-283, 2018.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 94. | | MANZINI, M. C.; PEREZ, K. R.; RISKE, K. A.; BOZELLI JUNIOR, J. C.; SANTOS, T. L.; SILVA, M. A. da; SARAIVA, G. K. V.; POLITI, M. J.; VALENTE, A. P.; ALMEIDA, F. C. L.; CHAIMOVICH, H.; RODRIGUES, M. A.; BEMQUERER, M. P.; SCHREIER, S.; CUCCOVIA, I. M. Peptide: lipid ratio and membrane surface charge determine the mechanismof action of the antimicrobial peptide BP100. Conformational and functional studies. Biochimica et Biophysica Acta, v. 1838, p. 1985-1999, 2014.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 95. | | ARCANJO, D. D. R.; VASCONCELOS, A. G.; COMERMA-STEFFENSEN, S. G.; JESUS, J. R.; SILVA, L. P. da; PIRES JÚNIOR, O. R.; COSTA-NETO, C. M.; OLIVEIRA, E. B.; MIGLIOLO, L.; FRANCO, O. L.; RESTINI, C. B. A.; PAULO, M.; BENDHACK, L. M.; BEMQUERER, M. P.; OLIVEIRA, A. P.; SIMONSEN, U.; LEITE, J. R. de S. de A. A novel vasoactive proline-rich oligopeptide from the skin secretion of the frog Brachycephalus ephippium. Plos One, v. 10, dez. 2015. (Open Access)| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 96. | | ARCANJO, D.; BEZERRA, I.; MOREIRA, D.; STEFFENSEN, S. G. C.; VASCONCELOS, A.; PRAT-DURAN, J.; TEIXEIRA, C.; GOMES, P.; CARDOSO-TEIXEIRA, A. C.; COELHO-DE-SOUZA, A.; BEMQUERER, M. P.; CHRISTENSEN, M. B.; WIMMER, R.; OLIVEIRA, A.; RAMOS, R.; SIMONSEN, U.; LEITE, J. R. Molecular dynamics and functional studies suggest brachyNH2 by binding to argininosuccinate synthase, induces relaxation in rat small mesenteric arteries. Journal of Vascular Research, v. 60, suppl 1, p. 58, 2023. Edição dos Abstracts do European Society for Microcirculation Conference, Aarhus, Dinamarca, 2023.| Biblioteca(s): Embrapa Gado de Leite. |
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| 97. | | BENEVENTI, M. A.; SHINOZAKI, K. Y.; NAKASHIMA, K.; NEPOMUCENO, A. L.; CORREA, F. M.; ROLLA, A. A. P. de SÁ MANDEL, S. M.; BEMQUERER, M. P.; OLIVEIRA, G. R.; NAKASU, E. Y. T.; ROCHA, T. L.; GROSSI de SÁ, M. F. Molecular analysis and biosafety of transgenic soybean drougth tolerance. In: SIMPÓSIO BRASILEIRO DE GENÉTICA MOLECULAR DE PLANTAS, 2., 2009, Búzios. Programa e resumos. [S.l.]: SBG, 2009. p. 107.| Biblioteca(s): Embrapa Soja. |
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| 98. | | NUNES, L. O.; MUNHOZ, V. H. O.; SOUSA, A. A.; SOUZA, K. R. de; SANTOS, T. L.; BEMQUERER, M. P.; FERREIRA, D. E. C.; MAGALHÃES, M. T. Q. de; RESENDE, J. M.; ALCÂNTARA, A. F. C.; AISENBREY, C.; VELOSO, D. P.; BECHINGER, B.; VERLY, R. M. High-resolution structural profile of hylaseptin-4: aggregation, membrane topology and pH dependence of overall membrane binding process. Biochimica et Biophysica Acta (BBA) - Biomembranes, v. 1863, n. 5, 183581, 2021.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 99. | | SANTOS, D. M.; VERLY, R. M.; PILÓ VELOSO, D.; MARIA, M. de; CARVALHO, M. A. R. de; CISALPINO, P. S.; SOARES, B. M.; DINIZ, C. G.; FARIAS, L. M.; MOREIRA, D. F. F.; FRÉZARD, F.; BEMQUERER, M. P.; PIMENTA, A. M. C.; LIMA, M. E. de. LyeTx I, a potent antimicrobial peptide from the venom of the spider Lycosa erythrognatha. Amino Acids, v. 39, p. 135-144, 2010.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 100. | | PRADO, G. S.; BAMOGO, P. K. Z.; ABREU, J. A. C. de; GILLET, F. X.; SANTOS, V. O. dos; SILVA, M. C. M. da; BRIZARD, J. P.; BEMQUERER, M. P.; BANGRATZ, M.; BRUGIDOU, C.; SÉRÉMÉ, D.; SA, M. F. G. de; LACOMBE, S. Nicotiana benthamiana is a suitable transient system for high-level expression of an active inhibitor of cotton boll weevil alfa-amylase. BMC Biotechnology, v. 19, n. 1, p.15, 2019. Na publicação: Maria Cristina Mattar Silva, Maria Fatima Grossi-de-Sa.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| Registros recuperados : 106 | |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
29/01/2024 |
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Data da última atualização: |
30/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 3 |
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Autoria: |
OLIVEIRA, K. C. B. F.; FARIAS, E. A. de O.; TEIXEIRA, P. R. S.; BRANDÃO, V. S.; SÁBIO, R. M.; ARAÚJO, A. R. de; EATON, P.; BERTOLINO, L. C.; BEMQUERER, M. P.; BARUD, H. da S.; LEITE, J. R. de S. de A.; EIRAS, C. |
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Afiliação: |
KARLA COSTA BEZERRA FONTENELE OLIVEIRA, UNIVERSIDADE FEDERAL DO PIAUÍ; EMANUEL AIRTON DE OLIVEIRA FARIAS, UNIVERSIDADE FEDERAL DO PIAUÍ; PAULO RONALDO SOUSA TEIXEIRA, UNIVERSIDADE FEDERAL DO PIAUÍ; VITOR SCHWENCK BRANDÃO, CENTRO DE TECNOLOGIA MINERAL; RAFAEL MIGUEL SÁBIO, UNIVERSIDADE DE ARARAQUARA; ALYNE RODRIGUES DE ARAÚJO, UNIVERSIDADE FEDERAL DO PIAUÍ; PETER EATON, UNIVERSIDADE DO PORTO; LUIZ CARLOS BERTOLINO, CENTRO DE TECNOLOGIA MINERAL; MARCELO PORTO BEMQUERER, CNPGL; HERNANE DA SILVA BARUD, UNIVERSIDADE DE ARARAQUARA; JOSÉ ROBERTO DE SOUZA DE ALMEIDA LEITE, UNIVERSIDADE DE BRASÍLIA; CARLA EIRAS, UNIVERSIDADE FEDERAL DO PIAUÍ. |
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Título: |
Development of a nanostructured film containing palygorskite and dermaseptin 01 peptide for biotechnological applications. |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Clays and Clay Minerals, v. 71, p. 600-615, 2023. |
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DOI: |
https://doi.org/10.1007/s42860-023-00259-z |
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Idioma: |
Inglês |
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Conteúdo: |
Clay minerals are suitable matrices to anchor organic molecules such as antimicrobial peptides (AMPs) so that their bioactivity is maintained, enabling the formation of new materials with potential for new applications in biotechnology. The objective of the present study was to develop a nanostructured flm where the properties of palygorskite (Plg) were combined at the molecular level with Dermaseptin 01 (DRS 01), in which the clay mineral also served as a substrate for the immobilization of this peptide. The flms were prepared using the Layer-byLayer (LbL) self-assembly technique. Crude palygorskite without purifcation (Plg IN) was subjected to physical and chemical procedures to increase its adsorptive properties. The structure, chemical composition, and morphology of Plg were investigated by X-ray difraction (XRD), Fourier-transform infrared spectroscopy (FTIR), X-ray fuorescence spectrometry (XRF), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). LbL flms were adsorbed onto ITO (Indium Tin Oxide) and characterized electrochemically by cyclic voltammetry (CV), UV-Visible spectroscopy, and atomic force microscopy (AFM). For the ITO/DRS 01 and ITO/Plg/DRS 01 flms, an oxidation process at +0.77 V was observed, confrming that the DRS 01 maintained its electroactive behavior and intrinsic properties. The results also showed that Plg served as excellent support for the immobilization of DRS 01, increasing its concentration and availability in the flm form. This work reported immobilizing the DRS 01 peptide with Plg for the frst time in an ultrathin flm with bioactive properties. Thus, the flm developed can be explored for applications such as biosensor devices and antimicrobial coating materials as well as other biotechnological applications. MenosClay minerals are suitable matrices to anchor organic molecules such as antimicrobial peptides (AMPs) so that their bioactivity is maintained, enabling the formation of new materials with potential for new applications in biotechnology. The objective of the present study was to develop a nanostructured flm where the properties of palygorskite (Plg) were combined at the molecular level with Dermaseptin 01 (DRS 01), in which the clay mineral also served as a substrate for the immobilization of this peptide. The flms were prepared using the Layer-byLayer (LbL) self-assembly technique. Crude palygorskite without purifcation (Plg IN) was subjected to physical and chemical procedures to increase its adsorptive properties. The structure, chemical composition, and morphology of Plg were investigated by X-ray difraction (XRD), Fourier-transform infrared spectroscopy (FTIR), X-ray fuorescence spectrometry (XRF), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). LbL flms were adsorbed onto ITO (Indium Tin Oxide) and characterized electrochemically by cyclic voltammetry (CV), UV-Visible spectroscopy, and atomic force microscopy (AFM). For the ITO/DRS 01 and ITO/Plg/DRS 01 flms, an oxidation process at +0.77 V was observed, confrming that the DRS 01 maintained its electroactive behavior and intrinsic properties. The results also showed that Plg served as excellent support for the immobilization of DRS 01, increasing its concentration and availability in the ... Mostrar Tudo |
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Palavras-Chave: |
Cationic antimicrobial peptides; Fibrous clay minerals; Filme fino; Filme nanoestruturado; Mineral argiloso fibroso; Peptídeo antimicrobiano catiônico; Pithecopus hipocondria. |
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Thesagro: |
Biotecnologia; Peptídeo. |
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Categoria do assunto: |
W Química e Física |
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Marc: |
LEADER 02987naa a2200373 a 4500
001 2161436
005 2024-01-30
008 2023 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s42860-023-00259-z$2DOI
100 1 $aOLIVEIRA, K. C. B. F.
245 $aDevelopment of a nanostructured film containing palygorskite and dermaseptin 01 peptide for biotechnological applications.$h[electronic resource]
260 $c2023
520 $aClay minerals are suitable matrices to anchor organic molecules such as antimicrobial peptides (AMPs) so that their bioactivity is maintained, enabling the formation of new materials with potential for new applications in biotechnology. The objective of the present study was to develop a nanostructured flm where the properties of palygorskite (Plg) were combined at the molecular level with Dermaseptin 01 (DRS 01), in which the clay mineral also served as a substrate for the immobilization of this peptide. The flms were prepared using the Layer-byLayer (LbL) self-assembly technique. Crude palygorskite without purifcation (Plg IN) was subjected to physical and chemical procedures to increase its adsorptive properties. The structure, chemical composition, and morphology of Plg were investigated by X-ray difraction (XRD), Fourier-transform infrared spectroscopy (FTIR), X-ray fuorescence spectrometry (XRF), scanning electron microscopy (SEM), and transmission electron microscopy (TEM). LbL flms were adsorbed onto ITO (Indium Tin Oxide) and characterized electrochemically by cyclic voltammetry (CV), UV-Visible spectroscopy, and atomic force microscopy (AFM). For the ITO/DRS 01 and ITO/Plg/DRS 01 flms, an oxidation process at +0.77 V was observed, confrming that the DRS 01 maintained its electroactive behavior and intrinsic properties. The results also showed that Plg served as excellent support for the immobilization of DRS 01, increasing its concentration and availability in the flm form. This work reported immobilizing the DRS 01 peptide with Plg for the frst time in an ultrathin flm with bioactive properties. Thus, the flm developed can be explored for applications such as biosensor devices and antimicrobial coating materials as well as other biotechnological applications.
650 $aBiotecnologia
650 $aPeptídeo
653 $aCationic antimicrobial peptides
653 $aFibrous clay minerals
653 $aFilme fino
653 $aFilme nanoestruturado
653 $aMineral argiloso fibroso
653 $aPeptídeo antimicrobiano catiônico
653 $aPithecopus hipocondria
700 1 $aFARIAS, E. A. de O.
700 1 $aTEIXEIRA, P. R. S.
700 1 $aBRANDÃO, V. S.
700 1 $aSÁBIO, R. M.
700 1 $aARAÚJO, A. R. de
700 1 $aEATON, P.
700 1 $aBERTOLINO, L. C.
700 1 $aBEMQUERER, M. P.
700 1 $aBARUD, H. da S.
700 1 $aLEITE, J. R. de S. de A.
700 1 $aEIRAS, C.
773 $tClays and Clay Minerals$gv. 71, p. 600-615, 2023.
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