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Registro Completo
Biblioteca(s): |
Embrapa Caprinos e Ovinos; Embrapa Gado de Leite. |
Data corrente: |
12/01/2021 |
Data da última atualização: |
15/09/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
BRAGANÇA, G. M.; BATISTA, R. I. T. P.; SOUZA-FABJAN, J. M. G.; ALFRADIQUE, V. A. P.; ARASHIRO, E. K. N.; PINTO, P. H. N.; SANTOS, J. D. R.; CAMARGO, L. S. de A.; MENCHACA, A.; FONSECA, J. F. da; BRANDÃO, F. Z. |
Afiliação: |
Universidade Federal Fluminense; Universidade Federal Fluminense; Universidade Federal Fluminense; Universidade Federal Fluminense; Universidade Federal Fluminense; Universidade Federal Fluminense; Universidade Federal Fluminense; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; Fundacion IRAUy, Cno. Cruz del Sur, Montevideo, Uruguay; JEFERSON FERREIRA DA FONSECA, CNPC; Universidade Federal Fluminense. |
Título: |
Exogenous progestogens differentially alter gene expression of immature cumulus-oocyte complexes in sheep. |
Ano de publicação: |
2021 |
Fonte/Imprenta: |
Domestic Animal Endocrinology, v. 74, e106518, 2021. |
DOI: |
https://doi.org/10.1016/j.domaniend.2020.106518 |
Idioma: |
Inglês |
Conteúdo: |
Abstracts: This study evaluated the role of progesterone (P4) and medroxyprogesterone acetate (MAP) on the molecular status of immature cumulus-oocyte complexes (COCs) and the implications for oocyte quality in sheep. The number of viable COCs per ewe and the rate of COCs screened for developmental competence by brilliant cresyl blue positive (BCBþ) were similar (P > 0.05), respectively, across treatments (P4: 7.7 ± 0.7 and 4.7 ± 1.2; MAP: 5.7 ± 1.0 and 3.5 ± 2.3; and control: 5.7 ± 1.1 and 3.6 ± 2.4). The COCs' gene expression was altered by exogenous progestogens compared with the control group: markers of steroidogenic pathway (FSH receptor [FSHr], LH receptor [LHr], and estradiol receptor a) and of quality (zygote arrest 1, growth differentiation factor 9, and B-cell lymphoma 2) were in abundance in P4 (P < 0.05). In addition, reelin protein (RELN) was downregulated, and Bcl-2 was upregulated in MAP (P < 0.05). In the P4 vs MAP comparison, FSHr, LHr, and RELN genes were upregulated (P < 0.05) in the P4 group. In conclusion, P4 and MAP promoted dissimilar effects on transcriptome profiling of immature BCB-selected COCs, possibly due to the differences in the chemical structure of progestogens and concentrations of serum P4. Exogenous P4 impacted positively on the profile of genes related to oocyte quality. |
Palavras-Chave: |
Oocyte competence; Progestins. |
Thesagro: |
Ovelha; Ovino; Reprodução Animal. |
Thesaurus NAL: |
Ewes; Gene expression; Medroxyprogesterone; Oocytes; Progesterone; Small ruminants. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 02483naa a2200385 a 4500 001 2133342 005 2021-09-15 008 2021 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1016/j.domaniend.2020.106518$2DOI 100 1 $aBRAGANÇA, G. M. 245 $aExogenous progestogens differentially alter gene expression of immature cumulus-oocyte complexes in sheep.$h[electronic resource] 260 $c2021 520 $aAbstracts: This study evaluated the role of progesterone (P4) and medroxyprogesterone acetate (MAP) on the molecular status of immature cumulus-oocyte complexes (COCs) and the implications for oocyte quality in sheep. The number of viable COCs per ewe and the rate of COCs screened for developmental competence by brilliant cresyl blue positive (BCBþ) were similar (P > 0.05), respectively, across treatments (P4: 7.7 ± 0.7 and 4.7 ± 1.2; MAP: 5.7 ± 1.0 and 3.5 ± 2.3; and control: 5.7 ± 1.1 and 3.6 ± 2.4). The COCs' gene expression was altered by exogenous progestogens compared with the control group: markers of steroidogenic pathway (FSH receptor [FSHr], LH receptor [LHr], and estradiol receptor a) and of quality (zygote arrest 1, growth differentiation factor 9, and B-cell lymphoma 2) were in abundance in P4 (P < 0.05). In addition, reelin protein (RELN) was downregulated, and Bcl-2 was upregulated in MAP (P < 0.05). In the P4 vs MAP comparison, FSHr, LHr, and RELN genes were upregulated (P < 0.05) in the P4 group. In conclusion, P4 and MAP promoted dissimilar effects on transcriptome profiling of immature BCB-selected COCs, possibly due to the differences in the chemical structure of progestogens and concentrations of serum P4. Exogenous P4 impacted positively on the profile of genes related to oocyte quality. 650 $aEwes 650 $aGene expression 650 $aMedroxyprogesterone 650 $aOocytes 650 $aProgesterone 650 $aSmall ruminants 650 $aOvelha 650 $aOvino 650 $aReprodução Animal 653 $aOocyte competence 653 $aProgestins 700 1 $aBATISTA, R. I. T. P. 700 1 $aSOUZA-FABJAN, J. M. G. 700 1 $aALFRADIQUE, V. A. P. 700 1 $aARASHIRO, E. K. N. 700 1 $aPINTO, P. H. N. 700 1 $aSANTOS, J. D. R. 700 1 $aCAMARGO, L. S. de A. 700 1 $aMENCHACA, A. 700 1 $aFONSECA, J. F. da 700 1 $aBRANDÃO, F. Z. 773 $tDomestic Animal Endocrinology$gv. 74, e106518, 2021.
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