Data/hora: 04/06/2024 15:48:01

Biblioteca(s): | Embrapa Caprinos e Ovinos.
Data corrente: | 29/09/2017
Data da última atualização: | 24/11/2022
Autoria: | SARAIVA, H. F. R. de A.; BATISTA, R. I. T. P.; ALFRADIQUE, V. A. P.; PINTO, P. H. N.; RIBEIRO, L. S.; OLIVEIRA, C. S.; SOUZA-FABJAN, J. M. G. de; CAMARGO, L. S. de A.; FONSECA, J. F. da; BRANDÃO, F. Z.
Afiliação: | Helena Fabiana Reis de Almeida Saraiva, Universidade Federal Fluminense (UFF) - Niteroi, RJ, Brazil; Ribrio Ivan Tavares Pereira Batista, Universidade Federal Fluminense (UFF) - Niterói, RJ, Brasil; Vivian Angélico Pereira Alfradique, UFF - Niteroi, RJ, Brazil; Pedro Henrique Nicolau Pinto, UFF - Niteroi, RJ, Brazil; Lilian Santos Ribeiro, UFF - Niteroi, RJ, Brazil; CLARA SLADE OLIVEIRA, CNPGL; Joanna Maria Gonçalves de Souza-Fabjan, UFF - Niteroi, RJ, Brazil; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; JEFERSON FERREIRA DA FONSECA, CNPC; Felipe Zandonadi Brandão, UFF - Niteroi, RJ, Brazil.
Título: | l-carnitine supplementation during vitrification or warming of in vivo-produced ovine embryos does not affect embryonic survival rates, but alters CrAT and PRDX1 expression.
Ano de publicação: | 2017
Fonte/Imprenta: | Theriogenology, v. 105, p. 150-157, Jan. 2018.
DOI: | https://doi.org/10.1016/j.theriogenology.2017.09.022
Idioma: | Inglês
Conteúdo: | Abstract: L-carnitine is an antioxidant and beta-oxidation stimulator substance commonly used to improve metabolic performance of oocytes and embryos in in vitro systems. However, few studies have evaluated its beneficial effects in embryos produced in vivo. This study aimed to evaluate the effect of L-carnitine supplementation into vitrification or warming solutions on the post-warming character of day 6?7 in vivo-produced ovine embryos. L-carnitine (3.72 mM) was added to vitrification (Experiment 1) or warming solutions (Experiment 2). In experiments 1 and 2, the embryos were vitrified using straw and cryo-tip protocols, respectively. In vitro culture (IVC) of warmed embryos was performed for 72 h in order to evaluate survival rates, reactive oxygen species (ROS) levels, total cell number (TCN), number of apoptotic cells, apoptotic index evaluation, and gene expression analysis of carnitine palmitoyltransferase I and 2 (CPT1 and CPT2), carnitine O-acetyltransferase (CrAT), and peroxiredoxin-1 (PRDX1). In experiment 1, survival rate, ROS levels after 24 h of IVC, total cell number at 24 h and 72 h, apoptotic cells and apoptotic index at 72 h of IVC were similar in embryos vitrified in medium supplemented with LC or not. Gene expression analysis showed no differences in CPT1 and CPT2mRNA relative abundance in embryos of both experiments compared to fresh embryos (FE); however, CrAT was downregulated (p < 0.05) in C1, and PRDX1 was downregulated (p < 0.05) in both the control (C1) and L-carnitine (LC1) groups, compared to FE. Moreover, CrAT and PRDX1 were upregulated (p < 0.05) in C2, and CrAT was downregulated (p < 0.05) in LC2, in relation to FE. Although the short-term LC supplementation at 3.72 mM did not improve survival, and quality parameters of in vivo-produced ovine embryos, it could affect their quality at a molecular level. In conclusion, further investigations with different concentrations of LC and tools are needed for improvement of the efficiency of these strategies.
Palavras-Chave: | Animal embryos; ATP cycle; Drug effects; Mitochondrial genetics; Santa ines; Survival.
Thesagro: | Ovino.
Thesaurus Nal: | Carnitine; Cell culture; Cryopreservation; Ewes; Gene expression; In vitro culture; oocytes; Sheep; Vitrification.
Categoria do assunto: | L Ciência Animal e Produtos de Origem Animal
Registro original: | Embrapa Caprinos e Ovinos (CNPC)
Exemplares: | CNPC - (35055-1) - UPC - AP - DD -  -  -  - DIS