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| Registros recuperados : 32 | |
| 3. |  | MENNA, P.; BARCELLOS, F. G.; BATISTA, J. S. da S.; BANGEL, E.; HUNGRIA, M. Classificação taxonômica, com base no gene ribossomal 16S, de uma coleção de rizóbios recomendados para o uso em inoculantes comerciais no Brasil. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 27.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 11.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 9.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 6., 2006, Bonito, MS. A busca das raízes: anais. Dourados: Embrapa Agropecuária Oeste, 2006. 1 CD-ROM. (Embrapa Agropecuária Oeste. Documentos, 82).| Biblioteca(s): Embrapa Soja. |
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| 7. | | BATISTA, J. S. da S.; RODRIGUES, E. P.; TORRES, A. R.; GOMES, D. F.; HUNGRIA, M. Efeito da inoculação com Bradyrhizobium japonicum no proteoma de raízes de soja. In: CONGRESSO BRASILEIRO DE MICROBILOGIA, 26.; SIMPÓSIO INTERNACIONAL DE BACTÉRIAS LÁTICAS; ENCONTRO NACIONAL DE PROFESSORES DE MICROBIOLOGIA; SIMPÓSIO DE COLEÇÕES DE CULTURAS, 4., 2011, Foz do Iguaçu. Anais... São Paulo: SBM, 2011. Resumo, 776-1.| Biblioteca(s): Embrapa Soja. |
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| 8. | | CARVALHO, G. A. B de; BATISTA, J. S. da S.; MARCELINO, F. C.; HUNGRIA, M. Efeito da inoculação da estirpe CPAC 15 de Bradyrhizobium japonicum na expressão gênica de raízes de soja. In: CONGRESSO BRASILEIRO DE MICROBILOGIA, 26.; SIMPÓSIO INTERNACIONAL DE BACTÉRIAS LÁTICAS; ENCONTRO NACIONAL DE PROFESSORES DE MICROBIOLOGIA; SIMPÓSIO DE COLEÇÕES DE CULTURAS, 4., 2011, Foz do Iguaçu. Anais... São Paulo: SBM, 2011. Resumo, 723-1.| Biblioteca(s): Embrapa Soja. |
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| 11. | | MENNA, P.; BATISTA, J. S. da S.; BARCELLOS, F. G.; BANGEL, E.; HUNGRIA, M. Diversidade de estirpes de Bradyrhizobium isoladas de diferentes leguminosas, com base na análise do gene ribossomal 16S e dos genes de nodulação nodY/KA. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 2., 2006, Londrina. Resumos expandidos. Londrina: Embrapa Soja, 2006. p.126-131. (Embrapa Soja. Documentos, 276).| Biblioteca(s): Embrapa Soja. |
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| 13. | | GOMES, D. F.; BATISTA, J. S. da S.; SCHIAVON, A. L.; ANDRADE, D. S.; HUNGRIA, M. Proteomic profiling of Rhizobium tropici PRF 81: identification of conserved and specific responses to heat stress. BMC Microbiology, London, v. 12, n. 84, p. 1-12, May 2012.| Biblioteca(s): Embrapa Soja. |
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| 14. | | BARCELLOS, F. G.; MENNA, P.; BATISTA, J. S. da S.; HUNGRIA, M. Transferência horizontal de genes entre diferentes espécies de rizóbios nos solos dos cerrados. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 27.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 11.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 9.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 6., 2006, Bonito, MS. A busca das raízes: anais. Dourados: Embrapa Agropecuária Oeste, 2006. 1 CD-ROM. (Embrapa Agropecuária Oeste. Documentos, 82).| Biblioteca(s): Embrapa Soja. |
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| 18. | | BATISTA, J. S. da S.; BARCELLOS, F. G.; MENNA, P.; BALLATI, P. A.; HUNGRIA, M. Confirmação da transferência horizontal de genes simbióticos entre gêneros distintos de rizóbios pela caracterização da estirpe de Sinorhizobium fredii CPAC 402, isolada de nódulo de soja nos cerrados. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 31., 2007, Gramado. Conquistas e desafios da ciência do solo brasileira : livro de resumos... Gramado: UFRGS: SBCS, 2007. 1 CD-ROM. Pdf. 7324-1775.| Biblioteca(s): Embrapa Soja. |
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| 20. | | BATISTA, J. S. da S.; BARCELLOS, F. G.; MENNA, P.; BALLATI, P. A.; HUNGRIA, M. Caracterização da estirpe de Sinorhizobium fredii CPAC 402: provável evento de transferência lateral de genes simbióticos em solos dos Cerrados entre gêneros distintos de rizóbios. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 2., 2006, Londrina. Resumos expandidos. Londrina: Embrapa Soja, 2006. p. 132-138. (Embrapa Soja. Documentos, 276).| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 32 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
26/08/2014 |
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Data da última atualização: |
19/06/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
GOMES, D. F.; BATISTA, J. S. da S.; ROLLA, A. A. P.; SILVA, L. P. da; BLOCH, C.; GALLI-TARASAWA, L. V.; HUNGRIA, M. |
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Afiliação: |
DOUGLAS FABIANO GOMES, Departamento de Génetica UFPR; JESSIANE STEFÂNIA DA SILVA BATISTA, DEBIOGEM UEPG; AMANDA ALVES PAIVA ROLLA; LUCIANO PAULINO DA SILVA, CENARGEN; CARLOS BLOCH JUNIOR, Cenargen; LYGIA VITORIA GALLI TERASAWA, Departamento de Génetica UFPR; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
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Título: |
Proteomic analysis of free-living Bradyrhizobium diazoefficiens: highlighting potential determinants of a successful symbiosis |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
BMC Genomics, v. 15, n. 643, 2014. |
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DOI: |
10.1186/1471-2164-15-643 |
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Idioma: |
Português |
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Conteúdo: |
Strain CPAC 7 (=SEMIA 5080) was recently reclassified into the new species Bradyrhizobium diazoefficiens; due to its outstanding efficiency in fixing nitrogen, it has been used in commercial inoculants for application to crops of soybean [Glycine max (L.) Merr.] in Brazil and other South American countries. Although the efficiency of B. diazoefficiens inoculant strains is well recognized, few data on their protein expression are available. We provided a two-dimensional proteomic reference map of CPAC 7 obtained under free-living conditions, with the successful identification of 115 spots, representing 95 different proteins. The results highlighted the expression of molecular determinants potentially related to symbiosis establishment (e.g. inositol monophosphatase, IMPase), fixation of atmospheric nitrogen (N 2) (e.g. NifH) and defenses against stresses (e.g. chaperones). By using bioinformatic tools, it was possible to attribute probable functions to ten hypothetical proteins. For another ten proteins classified as? NO related COG? group, we analyzed by RT-qPCR the relative expression of their coding-genes in response to the nodulation-gene inducer genistein. Six of these genes were up-regulated, including blr0227, which may be related to polyhydroxybutyrate (PHB) biosynthesis and competitiveness for nodulation. The proteomic map contributed to the identification of several proteins of B. diazoefficiens under free-living conditions and our approach? combining bioinformatics and gene-expression assays ? resulted in new information about unknown genes that might play important roles in the establishment of the symbiosis with soybean. MenosStrain CPAC 7 (=SEMIA 5080) was recently reclassified into the new species Bradyrhizobium diazoefficiens; due to its outstanding efficiency in fixing nitrogen, it has been used in commercial inoculants for application to crops of soybean [Glycine max (L.) Merr.] in Brazil and other South American countries. Although the efficiency of B. diazoefficiens inoculant strains is well recognized, few data on their protein expression are available. We provided a two-dimensional proteomic reference map of CPAC 7 obtained under free-living conditions, with the successful identification of 115 spots, representing 95 different proteins. The results highlighted the expression of molecular determinants potentially related to symbiosis establishment (e.g. inositol monophosphatase, IMPase), fixation of atmospheric nitrogen (N 2) (e.g. NifH) and defenses against stresses (e.g. chaperones). By using bioinformatic tools, it was possible to attribute probable functions to ten hypothetical proteins. For another ten proteins classified as? NO related COG? group, we analyzed by RT-qPCR the relative expression of their coding-genes in response to the nodulation-gene inducer genistein. Six of these genes were up-regulated, including blr0227, which may be related to polyhydroxybutyrate (PHB) biosynthesis and competitiveness for nodulation. The proteomic map contributed to the identification of several proteins of B. diazoefficiens under free-living conditions and our approach? combining bioinformatics... Mostrar Tudo |
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Palavras-Chave: |
RT-qPCR; Two-dimensional proteomics. |
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Thesaurus NAL: |
Bradyrhizobium; nitrogen fixation; symbiosis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/160837/1/12864-2014-Article-6775.pdf
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Marc: |
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024 7 $a10.1186/1471-2164-15-643$2DOI
100 1 $aGOMES, D. F.
245 $aProteomic analysis of free-living Bradyrhizobium diazoefficiens$bhighlighting potential determinants of a successful symbiosis$h[electronic resource]
260 $c2014
520 $aStrain CPAC 7 (=SEMIA 5080) was recently reclassified into the new species Bradyrhizobium diazoefficiens; due to its outstanding efficiency in fixing nitrogen, it has been used in commercial inoculants for application to crops of soybean [Glycine max (L.) Merr.] in Brazil and other South American countries. Although the efficiency of B. diazoefficiens inoculant strains is well recognized, few data on their protein expression are available. We provided a two-dimensional proteomic reference map of CPAC 7 obtained under free-living conditions, with the successful identification of 115 spots, representing 95 different proteins. The results highlighted the expression of molecular determinants potentially related to symbiosis establishment (e.g. inositol monophosphatase, IMPase), fixation of atmospheric nitrogen (N 2) (e.g. NifH) and defenses against stresses (e.g. chaperones). By using bioinformatic tools, it was possible to attribute probable functions to ten hypothetical proteins. For another ten proteins classified as? NO related COG? group, we analyzed by RT-qPCR the relative expression of their coding-genes in response to the nodulation-gene inducer genistein. Six of these genes were up-regulated, including blr0227, which may be related to polyhydroxybutyrate (PHB) biosynthesis and competitiveness for nodulation. The proteomic map contributed to the identification of several proteins of B. diazoefficiens under free-living conditions and our approach? combining bioinformatics and gene-expression assays ? resulted in new information about unknown genes that might play important roles in the establishment of the symbiosis with soybean.
650 $aBradyrhizobium
650 $anitrogen fixation
650 $asymbiosis
653 $aRT-qPCR
653 $aTwo-dimensional proteomics
700 1 $aBATISTA, J. S. da S.
700 1 $aROLLA, A. A. P.
700 1 $aSILVA, L. P. da
700 1 $aBLOCH, C.
700 1 $aGALLI-TARASAWA, L. V.
700 1 $aHUNGRIA, M.
773 $tBMC Genomics$gv. 15, n. 643, 2014.
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