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| Registros recuperados : 32 | |
| 4. |  | MENNA, P.; BARCELLOS, F. G.; BATISTA, J. S. da S.; BANGEL, E.; HUNGRIA, M. Classificação taxonômica, com base no gene ribossomal 16S, de uma coleção de rizóbios recomendados para o uso em inoculantes comerciais no Brasil. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 27.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 11.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 9.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 6., 2006, Bonito, MS. A busca das raízes: anais. Dourados: Embrapa Agropecuária Oeste, 2006. 1 CD-ROM. (Embrapa Agropecuária Oeste. Documentos, 82).| Biblioteca(s): Embrapa Soja. |
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| 6. | | MENNA, P.; BATISTA, J. S. da S.; BARCELLOS, F. G.; BANGEL, E.; HUNGRIA, M. Diversidade de estirpes de Bradyrhizobium isoladas de diferentes leguminosas, com base na análise do gene ribossomal 16S e dos genes de nodulação nodY/KA. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 2., 2006, Londrina. Resumos expandidos. Londrina: Embrapa Soja, 2006. p.126-131. (Embrapa Soja. Documentos, 276).| Biblioteca(s): Embrapa Soja. |
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| 8. | | BATISTA, J. S. da S.; RODRIGUES, E. P.; TORRES, A. R.; GOMES, D. F.; HUNGRIA, M. Efeito da inoculação com Bradyrhizobium japonicum no proteoma de raízes de soja. In: CONGRESSO BRASILEIRO DE MICROBILOGIA, 26.; SIMPÓSIO INTERNACIONAL DE BACTÉRIAS LÁTICAS; ENCONTRO NACIONAL DE PROFESSORES DE MICROBIOLOGIA; SIMPÓSIO DE COLEÇÕES DE CULTURAS, 4., 2011, Foz do Iguaçu. Anais... São Paulo: SBM, 2011. Resumo, 776-1.| Biblioteca(s): Embrapa Soja. |
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| 9. | | CARVALHO, G. A. B de; BATISTA, J. S. da S.; MARCELINO, F. C.; HUNGRIA, M. Efeito da inoculação da estirpe CPAC 15 de Bradyrhizobium japonicum na expressão gênica de raízes de soja. In: CONGRESSO BRASILEIRO DE MICROBILOGIA, 26.; SIMPÓSIO INTERNACIONAL DE BACTÉRIAS LÁTICAS; ENCONTRO NACIONAL DE PROFESSORES DE MICROBIOLOGIA; SIMPÓSIO DE COLEÇÕES DE CULTURAS, 4., 2011, Foz do Iguaçu. Anais... São Paulo: SBM, 2011. Resumo, 723-1.| Biblioteca(s): Embrapa Soja. |
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| 11. | | BATISTA, J. S. da S.; BARCELLOS, F. G.; MENNA, P.; BALLATI, P. A.; HUNGRIA, M. Confirmação da transferência horizontal de genes simbióticos entre gêneros distintos de rizóbios pela caracterização da estirpe de Sinorhizobium fredii CPAC 402, isolada de nódulo de soja nos cerrados. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 31., 2007, Gramado. Conquistas e desafios da ciência do solo brasileira : livro de resumos... Gramado: UFRGS: SBCS, 2007. 1 CD-ROM. Pdf. 7324-1775.| Biblioteca(s): Embrapa Soja. |
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| 12. | | GOMES, D. F.; BATISTA, J. S. da S.; SCHIAVON, A. L.; ANDRADE, D. S.; HUNGRIA, M. Proteomic profiling of Rhizobium tropici PRF 81: identification of conserved and specific responses to heat stress. BMC Microbiology, London, v. 12, n. 84, p. 1-12, May 2012.| Biblioteca(s): Embrapa Soja. |
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| 18. | | BATISTA, J. S. da S.; BARCELLOS, F. G.; MENNA, P.; BALLATI, P. A.; HUNGRIA, M. Caracterização da estirpe de Sinorhizobium fredii CPAC 402: provável evento de transferência lateral de genes simbióticos em solos dos Cerrados entre gêneros distintos de rizóbios. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 2., 2006, Londrina. Resumos expandidos. Londrina: Embrapa Soja, 2006. p. 132-138. (Embrapa Soja. Documentos, 276).| Biblioteca(s): Embrapa Soja. |
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| 19. | | RODRIGUES, E. P.; TORRES, A. R.; BATISTA, J. S. da S. B.; HUERGO, L.; HUNGRIA, M. A simple, economical and reproducible protein extraction protocol for proteomics studies of soybean roots. Genetics and Molecular Biology, Ribeirão Preto, v. 35, n. 1, suppl., p. 348-352, May 2012.| Biblioteca(s): Embrapa Soja. |
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| 20. | | BARCELLOS, F. G.; MENNA, P.; BATISTA, J. S. da S.; HUNGRIA, M. Transferência horizontal de genes entre diferentes espécies de rizóbios nos solos dos cerrados. In: REUNIÃO BRASILEIRA DE FERTILIDADE DO SOLO E NUTRIÇÃO DE PLANTAS, 27.; REUNIÃO BRASILEIRA SOBRE MICORRIZAS, 11.; SIMPÓSIO BRASILEIRO DE MICROBIOLOGIA DO SOLO, 9.; REUNIÃO BRASILEIRA DE BIOLOGIA DO SOLO, 6., 2006, Bonito, MS. A busca das raízes: anais. Dourados: Embrapa Agropecuária Oeste, 2006. 1 CD-ROM. (Embrapa Agropecuária Oeste. Documentos, 82).| Biblioteca(s): Embrapa Soja. |
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| Registros recuperados : 32 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Soja. Para informações adicionais entre em contato com valeria.cardoso@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
15/09/2010 |
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Data da última atualização: |
03/08/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
BATISTA, J. S. DA S.; TORRES, A. R.; HUNGRIA, M. |
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Afiliação: |
JESIANE STEFÂNIA DA SILVA BATISTA, UEL; ADALGISA RIBEIRO TORRES; MARIANGELA HUNGRIA DA CUNHA, CNPSO. |
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Título: |
Towards a two-dimensional proteomic reference map of Bradyrhizobium japonicum CPAC 15: Spotlighting "hypothetical proteins". |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Proteomics, Weinheim, v. 10, n. 17, p. 3176-3189, Sept. 2010. |
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ISSN: |
1615-9861 |
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DOI: |
DOI 10.1002/pmic.201000092 |
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Idioma: |
Português |
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Conteúdo: |
The economic and ecological importance of the symbiosis of soybean with Bradyrhizobium japonicum strains is significant in several countries, particularly Brazil; however, up to now, only one complete and a draft genome for this species are available. In this study, we have obtained a proteomic reference map of B. japonicum strain CPAC 15 (=SEMIA 5079) ? used in commercial inoculants for application to soybean crops in Brazil ? grown under in vitro conditions. CPAC 15 belongs to the same serogroup as strain USDA 123, and both are known as the soybean bradyrhizobial strains with highest competitive and saprophytic known so far. To increase the precision of the proteomic map, we compared whole-cell 2-D protein gel-electrophoresis profiles of CPAC 15 and of two related strains. One-hundred and seventy representative spots, selected from the three profiles, were analyzed by MS. In total, 148 spots were successfully identified as cytoplasmic and periplasmic proteins belonging to diverse metabolic pathways, several of them related to the saprophytic and competitive abilities of CPAC 15. We attributed probable functions to 26 hypothetical proteins, including those involved in polyhydroxybutyrate metabolism, ?-lactamase, stress responses and aromatic compound degradation, all with high probability of being related to the saprophytic ability of CPAC 15. In addition, by providing valuable information about expressed proteins in B. japonicum in vitro, our results emphasize the importance of accurate functional annotation of uncharacterized expressed proteins, improving considerably our understanding of the legume-rhizobia symbiosis. MenosThe economic and ecological importance of the symbiosis of soybean with Bradyrhizobium japonicum strains is significant in several countries, particularly Brazil; however, up to now, only one complete and a draft genome for this species are available. In this study, we have obtained a proteomic reference map of B. japonicum strain CPAC 15 (=SEMIA 5079) ? used in commercial inoculants for application to soybean crops in Brazil ? grown under in vitro conditions. CPAC 15 belongs to the same serogroup as strain USDA 123, and both are known as the soybean bradyrhizobial strains with highest competitive and saprophytic known so far. To increase the precision of the proteomic map, we compared whole-cell 2-D protein gel-electrophoresis profiles of CPAC 15 and of two related strains. One-hundred and seventy representative spots, selected from the three profiles, were analyzed by MS. In total, 148 spots were successfully identified as cytoplasmic and periplasmic proteins belonging to diverse metabolic pathways, several of them related to the saprophytic and competitive abilities of CPAC 15. We attributed probable functions to 26 hypothetical proteins, including those involved in polyhydroxybutyrate metabolism, ?-lactamase, stress responses and aromatic compound degradation, all with high probability of being related to the saprophytic ability of CPAC 15. In addition, by providing valuable information about expressed proteins in B. japonicum in vitro, our results emphasize the importan... Mostrar Tudo |
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Thesagro: |
Bacteria; Bradyrhizobium Japonicum; Microbiologia; Soja. |
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Thesaurus NAL: |
Microsymbionts; Soybeans. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02370naa a2200241 a 4500
001 1862320
005 2017-08-03
008 2010 bl uuuu u00u1 u #d
022 $a1615-9861
024 7 $aDOI 10.1002/pmic.201000092$2DOI
100 1 $aBATISTA, J. S. DA S.
245 $aTowards a two-dimensional proteomic reference map of Bradyrhizobium japonicum CPAC 15$bSpotlighting "hypothetical proteins".
260 $c2010
520 $aThe economic and ecological importance of the symbiosis of soybean with Bradyrhizobium japonicum strains is significant in several countries, particularly Brazil; however, up to now, only one complete and a draft genome for this species are available. In this study, we have obtained a proteomic reference map of B. japonicum strain CPAC 15 (=SEMIA 5079) ? used in commercial inoculants for application to soybean crops in Brazil ? grown under in vitro conditions. CPAC 15 belongs to the same serogroup as strain USDA 123, and both are known as the soybean bradyrhizobial strains with highest competitive and saprophytic known so far. To increase the precision of the proteomic map, we compared whole-cell 2-D protein gel-electrophoresis profiles of CPAC 15 and of two related strains. One-hundred and seventy representative spots, selected from the three profiles, were analyzed by MS. In total, 148 spots were successfully identified as cytoplasmic and periplasmic proteins belonging to diverse metabolic pathways, several of them related to the saprophytic and competitive abilities of CPAC 15. We attributed probable functions to 26 hypothetical proteins, including those involved in polyhydroxybutyrate metabolism, ?-lactamase, stress responses and aromatic compound degradation, all with high probability of being related to the saprophytic ability of CPAC 15. In addition, by providing valuable information about expressed proteins in B. japonicum in vitro, our results emphasize the importance of accurate functional annotation of uncharacterized expressed proteins, improving considerably our understanding of the legume-rhizobia symbiosis.
650 $aMicrosymbionts
650 $aSoybeans
650 $aBacteria
650 $aBradyrhizobium Japonicum
650 $aMicrobiologia
650 $aSoja
700 1 $aTORRES, A. R.
700 1 $aHUNGRIA, M.
773 $tProteomics, Weinheim$gv. 10, n. 17, p. 3176-3189, Sept. 2010.
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