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Registro Completo |
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Biblioteca(s): |
Embrapa Pantanal. |
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Data corrente: |
09/08/1995 |
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Data da última atualização: |
05/04/2017 |
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Autoria: |
NANTULYA, V. M.; MUSOKE, A. J.; RURANGIRWA, F. R.; MOLOO, S. K. |
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Título: |
Resistance of cattle to tsetse-transmitted challenge with Trypanosoma brucei or Trypanosoma congolense after spontaneous recovery from syringe passaged infections. |
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Ano de publicação: |
1984 |
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Fonte/Imprenta: |
Infection and Immunity, v.43, n.2, p.735-738, 1984. |
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Idioma: |
Inglês |
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Conteúdo: |
Groups of cattle were inoculated intravenously with cloned populations of bloodstream forms of Trypanosoma brucei or Trypanosoma congolense. All five steers infected with T. brucei IL Tat 2.1 and six of the eight sters infected with T. congolense IL 13-E14 became aparsitemic within 16 and 32 weeks postinfection, respectively. Examination of sera from animals infected with T. brucei by indirect immunofluorescence and neutralization assays revealed the presence of antibodies against all the metacyclic variable antigen types (VATs) of the infecting clone. The neutralizing capacity of the sera increased with the course of infection from 1:10 at 2 monts to 1:100 at 3 to 4 months postinfection. The recovered animals were completely immune to challenge by Glossina morsitans subsp. centralis infected with clone IL Tat 2.1 which had initiated the infection, as well as wich another clone (IL Tat 2.2) belonging to the same serodeme but they were susceptible to a tsetse-transmited heterologous challenge with isolate STIB 367-H. Similar results were obtained with sera from T. congolense IL 13-E14-infected steers. The six steers inbfected with a different T. congolense IL Nat 3.1 clone did not recover spotaneously; however, 2 months postinfection, sera from five of them also contained neutralizing antibodies against IL Nat 3.1 metacyclic VATs. These results indicate that some of the bloodstream VATs that arise during the course of a chronic infection possess surface epitopes in their variable surface glycoproteins that are identical to those of the metacyclic VATs. It is suggested that in chronic infection, the infecting trypanosomes could exhaust their VAT repertoire, including those that cross-react with metacyclies, threby leading to both self-cure and subsequent immunity to homologous cyclically transmited challenge. MenosGroups of cattle were inoculated intravenously with cloned populations of bloodstream forms of Trypanosoma brucei or Trypanosoma congolense. All five steers infected with T. brucei IL Tat 2.1 and six of the eight sters infected with T. congolense IL 13-E14 became aparsitemic within 16 and 32 weeks postinfection, respectively. Examination of sera from animals infected with T. brucei by indirect immunofluorescence and neutralization assays revealed the presence of antibodies against all the metacyclic variable antigen types (VATs) of the infecting clone. The neutralizing capacity of the sera increased with the course of infection from 1:10 at 2 monts to 1:100 at 3 to 4 months postinfection. The recovered animals were completely immune to challenge by Glossina morsitans subsp. centralis infected with clone IL Tat 2.1 which had initiated the infection, as well as wich another clone (IL Tat 2.2) belonging to the same serodeme but they were susceptible to a tsetse-transmited heterologous challenge with isolate STIB 367-H. Similar results were obtained with sera from T. congolense IL 13-E14-infected steers. The six steers inbfected with a different T. congolense IL Nat 3.1 clone did not recover spotaneously; however, 2 months postinfection, sera from five of them also contained neutralizing antibodies against IL Nat 3.1 metacyclic VATs. These results indicate that some of the bloodstream VATs that arise during the course of a chronic infection possess surface epitopes in their vari... Mostrar Tudo |
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Thesaurus Nal: |
Trypanosoma brucei; Trypanosoma congolense. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02445naa a2200181 a 4500
001 1785403
005 2017-04-05
008 1984 bl --- 0-- u #d
100 1 $aNANTULYA, V. M.
245 $aResistance of cattle to tsetse-transmitted challenge with Trypanosoma brucei or Trypanosoma congolense after spontaneous recovery from syringe passaged infections.
260 $c1984
520 $aGroups of cattle were inoculated intravenously with cloned populations of bloodstream forms of Trypanosoma brucei or Trypanosoma congolense. All five steers infected with T. brucei IL Tat 2.1 and six of the eight sters infected with T. congolense IL 13-E14 became aparsitemic within 16 and 32 weeks postinfection, respectively. Examination of sera from animals infected with T. brucei by indirect immunofluorescence and neutralization assays revealed the presence of antibodies against all the metacyclic variable antigen types (VATs) of the infecting clone. The neutralizing capacity of the sera increased with the course of infection from 1:10 at 2 monts to 1:100 at 3 to 4 months postinfection. The recovered animals were completely immune to challenge by Glossina morsitans subsp. centralis infected with clone IL Tat 2.1 which had initiated the infection, as well as wich another clone (IL Tat 2.2) belonging to the same serodeme but they were susceptible to a tsetse-transmited heterologous challenge with isolate STIB 367-H. Similar results were obtained with sera from T. congolense IL 13-E14-infected steers. The six steers inbfected with a different T. congolense IL Nat 3.1 clone did not recover spotaneously; however, 2 months postinfection, sera from five of them also contained neutralizing antibodies against IL Nat 3.1 metacyclic VATs. These results indicate that some of the bloodstream VATs that arise during the course of a chronic infection possess surface epitopes in their variable surface glycoproteins that are identical to those of the metacyclic VATs. It is suggested that in chronic infection, the infecting trypanosomes could exhaust their VAT repertoire, including those that cross-react with metacyclies, threby leading to both self-cure and subsequent immunity to homologous cyclically transmited challenge.
650 $aTrypanosoma brucei
650 $aTrypanosoma congolense
700 1 $aMUSOKE, A. J.
700 1 $aRURANGIRWA, F. R.
700 1 $aMOLOO, S. K.
773 $tInfection and Immunity$gv.43, n.2, p.735-738, 1984.
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Registro original: |
Embrapa Pantanal (CPAP) |
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Origem |
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
09/03/2017 |
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Data da última atualização: |
17/04/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
BATISTA, A. G.; SILVA, J. K. da; CAZARIN, C. B. B.; BIASOTO, A. C. T.; SAWAYA, A. C. H. F.; PRADO, M. A.; MARÓSTICA JÚNIOR, M. R. |
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Afiliação: |
ANGELA GIOVANA BATISTA, UNICAMP; JULIANA KELLY DA SILVA, UNICAMP; CINTHIA B. BETIM CAZARIN, UNICAMP; ALINE TELLES BIASOTO MARQUES, CPATSA; ALEXANDRA CHRISTINE HELENA FRANKLAND SAWAYA, UNICAMP; MARCELO ALEXANDRE PRADO, UNICAMP; MARIO ROBERTO MARÓSTICA JÚNIOR, UNICAMP. |
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Título: |
Red-jambo (Syzygium malaccense): bioactive compounds in fruits and leaves. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
LWT - Food Science and Technology, v. 76, p. 284-291, 2017. |
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ISSN: |
0023-6438 |
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Idioma: |
Inglês |
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Conteúdo: |
Syzygium malaccens e is poorly studied regarding the bioactive compounds of fruits and leaves. The aim of this study was to determine proximate composition, phenolic compounds, carotenoids, and antioxidant capacity of the fruit parts and leaves of S. malaccense. The samples were extracted with different solvents in order to analyze phenolic compounds content (Folin-Ciocalteau and HPLC-DAD/FLD), flavonoids (reaction with AlCl3 and HPLC-DAD/FLD), anthocyanins (differential pH and HPLC-DAD, UPLC-ESI-MS/MS), total carotenoids (colorimetric method) and antioxidant capacity (hydrophilic and lipophilic-ORAC, FRAP, DPPH). The pulp demonstrated high amounts of soluble fibers and reducing sugars. The peel, seeds and leaves of S. malaccense showed great contents of phenolic compounds, flavonoids and carotenoids as well as antioxidant capacity. The anthocyanins found in the fruit were cyanidin-3-O-glucoside, cyanidin-3,5- O-diglucoside, and peonidin-3-O-glucoside. Polar bioactive compounds showed strong correlation to hydrophilic antioxidant capacity, while carotenoids did not correlate lipophilic-ORAC. |
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Palavras-Chave: |
Antioxidantes; Carotenóides; Composto bioativo; Compostos fenólicos; Jambo vermelho; Malay apple. |
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Thesagro: |
Myrtaceae. |
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Thesaurus NAL: |
Hydrolysis; Polyphenols; Syzygium malaccense. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/157424/1/Aline-2017.pdf
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Marc: |
LEADER 02029naa a2200325 a 4500
001 2066591
005 2017-04-17
008 2017 bl uuuu u00u1 u #d
022 $a0023-6438
100 1 $aBATISTA, A. G.
245 $aRed-jambo (Syzygium malaccense)$bbioactive compounds in fruits and leaves.$h[electronic resource]
260 $c2017
520 $aSyzygium malaccens e is poorly studied regarding the bioactive compounds of fruits and leaves. The aim of this study was to determine proximate composition, phenolic compounds, carotenoids, and antioxidant capacity of the fruit parts and leaves of S. malaccense. The samples were extracted with different solvents in order to analyze phenolic compounds content (Folin-Ciocalteau and HPLC-DAD/FLD), flavonoids (reaction with AlCl3 and HPLC-DAD/FLD), anthocyanins (differential pH and HPLC-DAD, UPLC-ESI-MS/MS), total carotenoids (colorimetric method) and antioxidant capacity (hydrophilic and lipophilic-ORAC, FRAP, DPPH). The pulp demonstrated high amounts of soluble fibers and reducing sugars. The peel, seeds and leaves of S. malaccense showed great contents of phenolic compounds, flavonoids and carotenoids as well as antioxidant capacity. The anthocyanins found in the fruit were cyanidin-3-O-glucoside, cyanidin-3,5- O-diglucoside, and peonidin-3-O-glucoside. Polar bioactive compounds showed strong correlation to hydrophilic antioxidant capacity, while carotenoids did not correlate lipophilic-ORAC.
650 $aHydrolysis
650 $aPolyphenols
650 $aSyzygium malaccense
650 $aMyrtaceae
653 $aAntioxidantes
653 $aCarotenóides
653 $aComposto bioativo
653 $aCompostos fenólicos
653 $aJambo vermelho
653 $aMalay apple
700 1 $aSILVA, J. K. da
700 1 $aCAZARIN, C. B. B.
700 1 $aBIASOTO, A. C. T.
700 1 $aSAWAYA, A. C. H. F.
700 1 $aPRADO, M. A.
700 1 $aMARÓSTICA JÚNIOR, M. R.
773 $tLWT - Food Science and Technology$gv. 76, p. 284-291, 2017.
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Embrapa Semiárido (CPATSA) |
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