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| Registros recuperados : 5 | |
| 1. |  | CRUVINEL, L. B.; BORGES, D. G. L.; NICARETTA, J. E.; BASTOS, T. S. A.; GAMA, R. D.; BORGES, F. de A.; LOPES, W. D. Z. Avaliação da eficácia da lasalocida e de alguns fatores epidemiológicos de Eimeria spp. parasitando bezerros Nelore mantidos em regime de pastejo. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 2, p. 121-128, fev. 2017.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 2. | | MORAES, D. M. C.; DUARTE, S. C.; BASTOS, T. S. A.; REZENDE, C. L. G.; LEANDRO, N. S. M.; CAFÉ, M. B.; STRINGHINI, J. H.; ANDRADE, M. A. Detection of Salmonella spp. by Conventional Bacteriology and by Quantitative Polymerase-Chain Reaction in Commercial Egg Structures. Brazilian Journal of Poultry Science, v. 18, n.1, p. 117-124, 2016.| Biblioteca(s): Embrapa Suínos e Aves. |
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| 3. | | MORAES, D. M. C.; ANDRADE, M. A.; DUARTE, S. C.; BASTOS, T. S. A.; ARNHOLD, E.; JAYME, V. de S.; NUNES, I. A. Phenotypic and molecular detection of Salmonella sp. on growing, rearing and production phases in a commercial groupof laying hens. Pesquisa Veterinária Brasileira, Brasília, DF, v. 36, n. 6, p. 503-508, junho. 2016.| Biblioteca(s): Embrapa Suínos e Aves; Embrapa Unidades Centrais. |
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| 4. | | CRUVINEL, L. B.; BASTOS, T. S. A.; NICARETTA, J. E.; COUTO, L. F. M.; BORGES, D. G. L.; BORGES, F. A.; SOARES, V. E.; LOPES, W. D. Z. Surtos consecutivos ocasionados por Eimeria zuernii em bezerros de corte de uma propriedade do estado de São Paulo. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 2, p. 277-284, fevereiro 2018 Título em inglês: Consecutive outbreaks caused by Eimeria zuernii in calves on a rural property located in the municipality of São João da Boa Vista, State of São Paulo, Brazil.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 5. | | FARIA, A. M.; SANTOS, A. da S.; FERREIRA, L. L.; BASTOS, T. S. A.; LOULY, C. C. B.; MATOS, M. P. C.; CONCEIÇÃO, E. C. da; MOURA, V. M. B. D. de. Acaricidal activity of ethanolic extracts of Stryphnodendron adstringens and Lafoensia pacari and oil resins from Copaifera sp. and Pterodon emarginatus against Sarcoptes scabiei var. suis. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 12, p. 1411-1415, dezembro 2017. Título em português: Atividade acaricida dos extratos etanólicos de Stryphnodendron adstringens Mart. e Lafoensia pacari A. St Hil., bem como das óleo-resinas de Copaifera sp. e Pterodon emarginatus Vogel, 1837 contra Sarcoptes scabiei...| Biblioteca(s): Embrapa Unidades Centrais. |
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| Registros recuperados : 5 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
15/06/2016 |
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Data da última atualização: |
15/06/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
MORAES, D. M. C.; DUARTE, S. C.; BASTOS, T. S. A.; REZENDE, C. L. G.; LEANDRO, N. S. M.; CAFÉ, M. B.; STRINGHINI, J. H.; ANDRADE, M. A. |
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Afiliação: |
DUNYA MARA CARDOSO MORAES, UFG/Escola de Veterinária e Zootecnia; SABRINA CASTILHO DUARTE, CNPSA; UFG/Escola de Veterinária e Zootecnia; UFG/Escola de Veterinária e Zootecnia; UFG/Escola de Veterinária e Zootecnia; UFG/Escola de Veterinária e Zootecnia; JOSÉ HENRIQUE STRINGHINI, UFG/Escola de Veterinária e Zootecnia; MARIA AUXILIADORA ANDRADE, UFG/Escola de Veterinária e Zootecnia. |
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Título: |
Detection of Salmonella spp. by Conventional Bacteriology and by Quantitative Polymerase-Chain Reaction in Commercial Egg Structures. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Brazilian Journal of Poultry Science, v. 18, n.1, p. 117-124, 2016. |
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DOI: |
http://dx.doi.org/10.1590/18069061-2015-006 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Conventional bacteriology techniques and quantitative polymerasechain reaction (qPCR) were applied to the eggshell, albumen, and yolk of washed and unwashed commercial white and brown eggs, to detect Salmonella spp. Pooled samples of eggshells, albumen, and yolk of white and brown eggs were collected at the poultry house and at the egg-storage room. Salmonella spp. was detected by conventional bacteriology in 5.4% (21/387) of analyzed samples and in 16% (68/387) by qPCR. In the 114 unwashed white eggs samples of eggshell, albumen and yolk, the bacterium was identified in 2.6% of the eggs (3/114) by conventional bacteriology and in 13.2% (15/114) by qPCR. In the 90 samples of washed eggs, 6.7% (6/90) were contaminated as detected by conventional bacteriology and 10.0% (9/90) by qPCR. In the 81 samples of unwashed brown eggs, Salmonella spp. was detected in 6.1% of the eggs (5/81) by conventional bacteriology and 27.2% (22/81) by qPCR. In the 102 samples of brown washed eggs, 6.9% (7/102) where positive by conventional bacteriology and 35.3% (16/102) by qPCR. All samples detected as positive by conventional bacteriology were also positive by qPCR. Salmonella Agona represented 18.2% (4/22) of identified serovars, Salmonella enterica subs. enterica O: 4.5 18.2% (4/22), Salmonella Schwarzengrund 18.2% (4/22), Salmonella Cerro 13.6% (3/22), Salmonella Anatum 13.6% (3/22), Salmonella Enteritidis 9.1% (2/22), Salmonella Johannesburg 4.5% (1/22), and Salmonella Corvallis 4.5% (1/22). The qPCR method provided better detection of Salmonella spp. in commercial eggs than conventional bacteriology. The conventional egg washing and disinfection procedures are not efficient to eliminate Salmonella. MenosAbstract: Conventional bacteriology techniques and quantitative polymerasechain reaction (qPCR) were applied to the eggshell, albumen, and yolk of washed and unwashed commercial white and brown eggs, to detect Salmonella spp. Pooled samples of eggshells, albumen, and yolk of white and brown eggs were collected at the poultry house and at the egg-storage room. Salmonella spp. was detected by conventional bacteriology in 5.4% (21/387) of analyzed samples and in 16% (68/387) by qPCR. In the 114 unwashed white eggs samples of eggshell, albumen and yolk, the bacterium was identified in 2.6% of the eggs (3/114) by conventional bacteriology and in 13.2% (15/114) by qPCR. In the 90 samples of washed eggs, 6.7% (6/90) were contaminated as detected by conventional bacteriology and 10.0% (9/90) by qPCR. In the 81 samples of unwashed brown eggs, Salmonella spp. was detected in 6.1% of the eggs (5/81) by conventional bacteriology and 27.2% (22/81) by qPCR. In the 102 samples of brown washed eggs, 6.9% (7/102) where positive by conventional bacteriology and 35.3% (16/102) by qPCR. All samples detected as positive by conventional bacteriology were also positive by qPCR. Salmonella Agona represented 18.2% (4/22) of identified serovars, Salmonella enterica subs. enterica O: 4.5 18.2% (4/22), Salmonella Schwarzengrund 18.2% (4/22), Salmonella Cerro 13.6% (3/22), Salmonella Anatum 13.6% (3/22), Salmonella Enteritidis 9.1% (2/22), Salmonella Johannesburg 4.5% (1/22), and Salmonella Corvallis 4.... Mostrar Tudo |
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Palavras-Chave: |
QPCR. |
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Thesagro: |
Bacteriologia; Casca de ovo; Gema de ovo; Salmonella. |
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Thesaurus NAL: |
Egg albumen; Egg shell; Egg yolk; Quantitative polymerase chain reaction. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02730naa a2200325 a 4500
001 2047116
005 2016-06-15
008 2016 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1590/18069061-2015-006$2DOI
100 1 $aMORAES, D. M. C.
245 $aDetection of Salmonella spp. by Conventional Bacteriology and by Quantitative Polymerase-Chain Reaction in Commercial Egg Structures.$h[electronic resource]
260 $c2016
520 $aAbstract: Conventional bacteriology techniques and quantitative polymerasechain reaction (qPCR) were applied to the eggshell, albumen, and yolk of washed and unwashed commercial white and brown eggs, to detect Salmonella spp. Pooled samples of eggshells, albumen, and yolk of white and brown eggs were collected at the poultry house and at the egg-storage room. Salmonella spp. was detected by conventional bacteriology in 5.4% (21/387) of analyzed samples and in 16% (68/387) by qPCR. In the 114 unwashed white eggs samples of eggshell, albumen and yolk, the bacterium was identified in 2.6% of the eggs (3/114) by conventional bacteriology and in 13.2% (15/114) by qPCR. In the 90 samples of washed eggs, 6.7% (6/90) were contaminated as detected by conventional bacteriology and 10.0% (9/90) by qPCR. In the 81 samples of unwashed brown eggs, Salmonella spp. was detected in 6.1% of the eggs (5/81) by conventional bacteriology and 27.2% (22/81) by qPCR. In the 102 samples of brown washed eggs, 6.9% (7/102) where positive by conventional bacteriology and 35.3% (16/102) by qPCR. All samples detected as positive by conventional bacteriology were also positive by qPCR. Salmonella Agona represented 18.2% (4/22) of identified serovars, Salmonella enterica subs. enterica O: 4.5 18.2% (4/22), Salmonella Schwarzengrund 18.2% (4/22), Salmonella Cerro 13.6% (3/22), Salmonella Anatum 13.6% (3/22), Salmonella Enteritidis 9.1% (2/22), Salmonella Johannesburg 4.5% (1/22), and Salmonella Corvallis 4.5% (1/22). The qPCR method provided better detection of Salmonella spp. in commercial eggs than conventional bacteriology. The conventional egg washing and disinfection procedures are not efficient to eliminate Salmonella.
650 $aEgg albumen
650 $aEgg shell
650 $aEgg yolk
650 $aQuantitative polymerase chain reaction
650 $aBacteriologia
650 $aCasca de ovo
650 $aGema de ovo
650 $aSalmonella
653 $aQPCR
700 1 $aDUARTE, S. C.
700 1 $aBASTOS, T. S. A.
700 1 $aREZENDE, C. L. G.
700 1 $aLEANDRO, N. S. M.
700 1 $aCAFÉ, M. B.
700 1 $aSTRINGHINI, J. H.
700 1 $aANDRADE, M. A.
773 $tBrazilian Journal of Poultry Science$gv. 18, n.1, p. 117-124, 2016.
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