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Registro Completo |
Biblioteca(s): |
Embrapa Cerrados. |
Data corrente: |
31/01/2014 |
Data da última atualização: |
04/02/2014 |
Autoria: |
BORGHETTI, F.; LIMA, E. C. de; SILVA, L. de C. R. |
Afiliação: |
FABIAN BORGHETTI, Universidade de Brasília, Departamento de Botânica, Brasília, DF, Brasil.; ELISA COUTINHO DE LIMA, Universidade de Brasília, Departamento de Botânica, Brasília, DF, Brasil.; LUCAS DE CARVALHO RAMOS SILVA. |
Título: |
A simple procedure for the purification of active fractions in aqueous extracts of plants with allelopathic properties. |
Ano de publicação: |
2013 |
Fonte/Imprenta: |
Acta Botanica Brasilica, Feira de Santana, v. 27, n. 1, p. 50-53, mar. 2013. |
ISSN: |
0102-3306 |
Idioma: |
Português |
Conteúdo: |
Most studies conducted to test the allelopathic activity of plant parts have made use of water as solvent. However, the presence of polar, water-soluble substances, such as proteins and carbohydrates, tends to hamper the purifi cation of active compounds. In this study, we present a simple purifi cation procedure that separates the active fraction of the extract from the undesirable substances, thus facilitating the search for active molecules through standard chromatographic methods. Aqueous leaf extracts of three Cerrado species (Caryocar brasiliense, Qualea parvifl ora and Eugenia dysenterica) were prepared at 5% concentration (w/v) and stored at 4°C (crude extracts). Aft er 24 h, these solutions were fi ltered and freeze-dried. Th e powder obtained was dissolved in methanol, fi ltered again, evaporated and dissolved in water for bioassays (purifi ed extracts). For the bioassays, seedlings of Sesamum indicum were grown for fi ve days in aqueous solutions prepared from crude and purifi ed extracts at concentrations ranging from 0.1% to 1.0% (w/v). Seedling growth in distilled water was set as a control. In comparison with the control, we found that test solutions prepared from both crude and purifi ed extracts signifi cantly inhibited sesame seedling growth. However, solutions prepared from purifi ed extracts were two to ten times more inhibitory to seedling growth than were those prepared from crude extracts. Th e inhibition of root growth ranged from 35% to 77%, depending on the plant species, at a concentration as low as 0.1%. Roots were more aff ected than were shoots. Th e eff ects of purifi ed extracts on seedling
morphology were similar to those observed when crude extracts were employed, indicating that the procedure of purifi cation of crude extracts did not interfere with the mode of action of the active substances. MenosMost studies conducted to test the allelopathic activity of plant parts have made use of water as solvent. However, the presence of polar, water-soluble substances, such as proteins and carbohydrates, tends to hamper the purifi cation of active compounds. In this study, we present a simple purifi cation procedure that separates the active fraction of the extract from the undesirable substances, thus facilitating the search for active molecules through standard chromatographic methods. Aqueous leaf extracts of three Cerrado species (Caryocar brasiliense, Qualea parvifl ora and Eugenia dysenterica) were prepared at 5% concentration (w/v) and stored at 4°C (crude extracts). Aft er 24 h, these solutions were fi ltered and freeze-dried. Th e powder obtained was dissolved in methanol, fi ltered again, evaporated and dissolved in water for bioassays (purifi ed extracts). For the bioassays, seedlings of Sesamum indicum were grown for fi ve days in aqueous solutions prepared from crude and purifi ed extracts at concentrations ranging from 0.1% to 1.0% (w/v). Seedling growth in distilled water was set as a control. In comparison with the control, we found that test solutions prepared from both crude and purifi ed extracts signifi cantly inhibited sesame seedling growth. However, solutions prepared from purifi ed extracts were two to ten times more inhibitory to seedling growth than were those prepared from crude extracts. Th e inhibition of root growth ranged from 35% to 77%, dependin... Mostrar Tudo |
Palavras-Chave: |
Extratos aquosos de plantas; Freeze-drying; Leaf extract; Processo; Purification. |
Thesagro: |
Alelopatia; Cerrado; Purificação. |
Thesaurus Nal: |
allelopathy. |
Categoria do assunto: |
-- |
Marc: |
LEADER 02622nam a2200253 a 4500 001 1978172 005 2014-02-04 008 2013 bl uuuu u0uu1 u #d 022 $a0102-3306 100 1 $aBORGHETTI, F. 245 $aA simple procedure for the purification of active fractions in aqueous extracts of plants with allelopathic properties. 260 $aActa Botanica Brasilica, Feira de Santana, v. 27, n. 1, p. 50-53, mar. 2013.$c2013 520 $aMost studies conducted to test the allelopathic activity of plant parts have made use of water as solvent. However, the presence of polar, water-soluble substances, such as proteins and carbohydrates, tends to hamper the purifi cation of active compounds. In this study, we present a simple purifi cation procedure that separates the active fraction of the extract from the undesirable substances, thus facilitating the search for active molecules through standard chromatographic methods. Aqueous leaf extracts of three Cerrado species (Caryocar brasiliense, Qualea parvifl ora and Eugenia dysenterica) were prepared at 5% concentration (w/v) and stored at 4°C (crude extracts). Aft er 24 h, these solutions were fi ltered and freeze-dried. Th e powder obtained was dissolved in methanol, fi ltered again, evaporated and dissolved in water for bioassays (purifi ed extracts). For the bioassays, seedlings of Sesamum indicum were grown for fi ve days in aqueous solutions prepared from crude and purifi ed extracts at concentrations ranging from 0.1% to 1.0% (w/v). Seedling growth in distilled water was set as a control. In comparison with the control, we found that test solutions prepared from both crude and purifi ed extracts signifi cantly inhibited sesame seedling growth. However, solutions prepared from purifi ed extracts were two to ten times more inhibitory to seedling growth than were those prepared from crude extracts. Th e inhibition of root growth ranged from 35% to 77%, depending on the plant species, at a concentration as low as 0.1%. Roots were more aff ected than were shoots. Th e eff ects of purifi ed extracts on seedling morphology were similar to those observed when crude extracts were employed, indicating that the procedure of purifi cation of crude extracts did not interfere with the mode of action of the active substances. 650 $aallelopathy 650 $aAlelopatia 650 $aCerrado 650 $aPurificação 653 $aExtratos aquosos de plantas 653 $aFreeze-drying 653 $aLeaf extract 653 $aProcesso 653 $aPurification 700 1 $aLIMA, E. C. de 700 1 $aSILVA, L. de C. R.
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Registro original: |
Embrapa Cerrados (CPAC) |
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Registro Completo
Biblioteca(s): |
Embrapa Amazônia Oriental. |
Data corrente: |
17/03/2006 |
Data da última atualização: |
29/11/2022 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
BENCHIMOL, R. L.; SUTTON, J. C.; BASTOS, C. N.; DIAS-FILHO, M. B. |
Afiliação: |
RUTH LINDA BENCHIMOL, CPATU; UNIVERSITY OF GUELPH; CEPLAC; MOACYR BERNARDINO DIAS FILHO, CPATU. |
Título: |
Piper aduncum Helps fusariosis control and growth promotion in black pepper. |
Ano de publicação: |
2003 |
Fonte/Imprenta: |
Fitopatologia Brasileira, v. 28, p. S346, ago. 2003. Suplemento. |
Idioma: |
Inglês |
Palavras-Chave: |
Controle de doença; Pimenta-do-reino. |
Thesagro: |
Crescimento. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/102231/1/S346.pdf
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Marc: |
LEADER 00549nam a2200169 a 4500 001 1401932 005 2022-11-29 008 2003 bl uuuu u00u1 u #d 100 1 $aBENCHIMOL, R. L. 245 $aPiper aduncum Helps fusariosis control and growth promotion in black pepper.$h[electronic resource] 260 $aFitopatologia Brasileira, v. 28, p. S346, ago. 2003. Suplemento.$c2003 650 $aCrescimento 653 $aControle de doença 653 $aPimenta-do-reino 700 1 $aSUTTON, J. C. 700 1 $aBASTOS, C. N. 700 1 $aDIAS-FILHO, M. B.
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