Data/hora: 16/06/2024 07:46:02

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	22/03/2011	
Data da última atualização: 	05/06/2023	
Autoria: 	COSTA, F. M.; BASTIANEL, M.; FREITAS-ASTUA, J.; KUBO, K. S.; MACHADO, M. A.; GONDIM JUNIOR, M. G. C.	
Afiliação: 	F. M. Costa, UFRPE; Marinês Bastianel, APTA; JULIANA DE FREITAS ASTUA, CNPMF; Karen Sumire Kubo, IAC; Marcos Antonio Machado, APTA; Manoel Guedes Corrêa Gondim Júnior, APTA.	
Título: 	Detection of coffee ringspot virus (CoRSV) in the mite vector by RT-qPCR.	
Ano de publicação: 	2010	
Fonte/Imprenta: 	In:INTERNATIONAL CONGRESS OF ACAROLOGY, 13., 2010, Recife. Abstracts book... Recife: CNPq: FACEPE: Banco do Nordeste: TWAS, 2010. p. 61-62.	
Idioma: 	Inglês	
Notas: 	Abstract 109	
Conteúdo: 	The mite Brevipalpus phoenicis (Geijskes, 1939) (Acari: Tenuipalpidae) is responsible for the transmission of coffee ringspot. There is a great interest in the study of Brevipalpus transmitted viruses (BrTVs), and the interaction between them, their vectors and hosts. Electron microscopy analyses suggest that CoRSV replicates in the mite tissue, what would characterize a persistent propagative virus-vector relationship. This study aimed to establish a transcriptase quantitative polymerase chain reaction (RT-qPCR) method to evaluate and compare the replication capacity of the virus in the vector. Total RNA was extracted from a sample of 100 mites reared in symptomatic plants for CoRSV using two different methods: CTAB and Nucleo Spin RNA XS Kit (Macherey-Nagel) and quantified in a NanoDrop 8000 (Thermo Scientific).	
Thesagro: 	Brevipalpus Phoenicis; Vírus.	
Categoria do assunto: 	X Pesquisa, Tecnologia e Engenharia	
URL: 	http://ainfo.cnptia.embrapa.br/digital/bitstream/item/31193/1/13CongASTUAID27574.pdf	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
Exemplares: 	CNPMF - (27574-1) - UPC - RA - DD - Publicação digital -  -  - DIS