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| Registros recuperados : 167 | |
| 9. |  | CARDOSO, M. S.; MARCELINO, F. C.; BARROS, E. G. de. Validação Ddos genes da W-gliadina e da glutenina como referências endógenas para a detecção e quantificação e resíduos de transgênicos em trigo (Triticum aestivum). In: CONGRESSO BRASILEIRO DE BIOSSEGURANÇA, 5.; SIMPÓSIO LATINO AMERICANO DE PRODUTOS TRANSGÊNICOS, 5.; SEMINÁRIO DE BIOENERGIA, 1.; SIMPÓSIO DE POPULARIZAÇÃO DA BIOTECNOLOGIA, 2.; MOSTRA DE BIOTECNOLOGIA PARA A SOCIEDADE, 1., 2007, Ouro Preto, MG. Anais dos eventos... Rio de Janeiro: Associação Nacional de Biossegurança, 2007. p.121.| Biblioteca(s): Embrapa Soja; Embrapa Trigo. |
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| 12. | | PINTO, M. de O.; GOOD-GOD, P. I. V.; MOREIRA, M. A.; BARROS, E. G. de. Associação de marcadores moleculares SNP com o conteúdo de ácido linolênico em sementes de soja. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 48, n. 3, p. 263-269, mar. 2013.| Biblioteca(s): Embrapa Milho e Sorgo; Embrapa Unidades Centrais. |
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| 14. | | RIBEIRO, C. A. G.; TANURE, J. P. M.; MACIEL, T. E. F.; BARROS, E. G. de. Molecular characterization of soybean cultivars by microsatellite markers with universal tail sequence. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 48, n. 3, p. 270-279, mar. 2013. Título em português: Caracterização molecular de cultivares de soja por meio de marcadores microssatélites com sequência de cauda universal.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 19. | | SOUZA, T. L. P. O.; ALZATE-MARIN, A. L.; FALEIRO, F. G.; BARROS, E. G. de. Pathosystem common bean-uromyces appendiculatus: host resistance, pathogen specialization, and breeding for rust resistance. Pest Technology, v. 2, n. 2, p. 56-69, 2008.| Biblioteca(s): Embrapa Cerrados. |
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| 20. | | FALEIRO, F. G.; CRUZ, C. D.; CASTRO, C. de; MOREIRA, M. A.; BARROS, E. G. de. Comparação de blocos casualizados e testemunhas intercalares na estimação de parâmetros genéticos em feijoeiro Pesquisa Agropecuária Brasileira, Brasília, DF, v. 37, n. 12, p. 1675-1680, dez. 2002 Título em inglês: Matching of random blocks and intercalated checks for genetic parameters estimation in common bean.| Biblioteca(s): Embrapa Cerrados; Embrapa Unidades Centrais. |
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| Registros recuperados : 167 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
01/09/2014 |
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Data da última atualização: |
09/12/2014 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
MÜLLER, B. S. de F.; SAKAMOTO, T.; MENEZES, I. P. P. de; PRADO, G. S.; MARTINS, W. S.; BRONDANI, C.; BARROS, E. G. de; VIANELLO, R. P. |
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Afiliação: |
BÁRBARA SALOMÃO DE FARIA MÜLLER, UFV; TETSU SAKAMOTO, UFMG; IVANDILSON PESSOA PINTO DE MENEZES, INSTITUTO FEDERAL GOIANO, Urutaí-GO; GUILHERME SOUZA PRADO, bolsista CNPAF; WELLINGTON SANTOS MARTINS, UFG; CLAUDIO BRONDANI, CNPAF; EVERALDO GONÇALVES DE BARROS, UNIVERSIDADE CATÓLICA DE BRASÍLIA; ROSANA PEREIRA VIANELLO, CNPAF. |
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Título: |
Analysis of BAC-end sequences in common bean (Phaseolus vulgaris L.) towards the development and characterization of long motifs SSRs. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
Plant Molecular Biology, Dordrecht, v. 86, n. 4/5, p. 455-470, Nov. 2014. |
|
DOI: |
10.1007/s11103-014-0240-7 |
|
Idioma: |
Inglês |
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Conteúdo: |
The increasing volume of genomic data on the Phaseolus vulgaris species have contributed to its importance as a model genetic species and positively affected the investigation of other legumes of scientific and economic value. To expand and gain a more in-depth knowledge of the common bean genome, the ends of a number of bacterial artificial chromosome (BAC) were sequenced, annotated and the presence of repetitive sequences was determined. In total, 52,270 BESs (BAC-end sequences), equivalent to 32 Mbp (~6 %) of the genome, were processed. In total, 3,789 BES-SSRs were identified, with a distribution of one SSR (simple sequence repeat) per 8.36 kbp and 2,000 were suitable for the development of SSRs, of which 194 were evaluated in low-resolution screening. From 40 BES-SSRs based on long motifs SSRs (≥trinucleotides) analyzed in high-resolution genotyping, 34 showed an equally good amplification for the Andean and for the Mesoamerican genepools, exhibiting an average gene diversity (H E) of 0.490 and 5.59 alleles/locus, of which six classified as Class I showed a H E ≥ 0.7. The PCoA and structure analysis allowed to discriminate the gene pools (K = 2, FST = 0.733). From the 52,270 BESs, 2 % corresponded to transcription factors and 3 % to transposable elements. Putative functions for 24,321 BESs were identified and for 19,363 were assigned functional categories (gene ontology). This study identified highly polymorphic BES-SSRs containing tri- to hexanucleotides motifs and bringing together relevant genetic characteristics useful for breeding programs. Additionally, the BESs were incorporated into the international genome-sequencing project for the common bean. MenosThe increasing volume of genomic data on the Phaseolus vulgaris species have contributed to its importance as a model genetic species and positively affected the investigation of other legumes of scientific and economic value. To expand and gain a more in-depth knowledge of the common bean genome, the ends of a number of bacterial artificial chromosome (BAC) were sequenced, annotated and the presence of repetitive sequences was determined. In total, 52,270 BESs (BAC-end sequences), equivalent to 32 Mbp (~6 %) of the genome, were processed. In total, 3,789 BES-SSRs were identified, with a distribution of one SSR (simple sequence repeat) per 8.36 kbp and 2,000 were suitable for the development of SSRs, of which 194 were evaluated in low-resolution screening. From 40 BES-SSRs based on long motifs SSRs (≥trinucleotides) analyzed in high-resolution genotyping, 34 showed an equally good amplification for the Andean and for the Mesoamerican genepools, exhibiting an average gene diversity (H E) of 0.490 and 5.59 alleles/locus, of which six classified as Class I showed a H E ≥ 0.7. The PCoA and structure analysis allowed to discriminate the gene pools (K = 2, FST = 0.733). From the 52,270 BESs, 2 % corresponded to transcription factors and 3 % to transposable elements. Putative functions for 24,321 BESs were identified and for 19,363 were assigned functional categories (gene ontology). This study identified highly polymorphic BES-SSRs containing tri- to hexanucleotides mo... Mostrar Tudo |
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Thesagro: |
Feijão; Genética molecular; Phaseolus vulgaris. |
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Thesaurus NAL: |
Beans; Fabaceae; Genomics; Microsatellite repeats; Molecular genetics; Transcription factors; Transposons. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
|
Marc: |
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001 1993888
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008 2014 bl uuuu u00u1 u #d
024 7 $a10.1007/s11103-014-0240-7$2DOI
100 1 $aMÜLLER, B. S. de F.
245 $aAnalysis of BAC-end sequences in common bean (Phaseolus vulgaris L.) towards the development and characterization of long motifs SSRs.$h[electronic resource]
260 $c2014
520 $aThe increasing volume of genomic data on the Phaseolus vulgaris species have contributed to its importance as a model genetic species and positively affected the investigation of other legumes of scientific and economic value. To expand and gain a more in-depth knowledge of the common bean genome, the ends of a number of bacterial artificial chromosome (BAC) were sequenced, annotated and the presence of repetitive sequences was determined. In total, 52,270 BESs (BAC-end sequences), equivalent to 32 Mbp (~6 %) of the genome, were processed. In total, 3,789 BES-SSRs were identified, with a distribution of one SSR (simple sequence repeat) per 8.36 kbp and 2,000 were suitable for the development of SSRs, of which 194 were evaluated in low-resolution screening. From 40 BES-SSRs based on long motifs SSRs (≥trinucleotides) analyzed in high-resolution genotyping, 34 showed an equally good amplification for the Andean and for the Mesoamerican genepools, exhibiting an average gene diversity (H E) of 0.490 and 5.59 alleles/locus, of which six classified as Class I showed a H E ≥ 0.7. The PCoA and structure analysis allowed to discriminate the gene pools (K = 2, FST = 0.733). From the 52,270 BESs, 2 % corresponded to transcription factors and 3 % to transposable elements. Putative functions for 24,321 BESs were identified and for 19,363 were assigned functional categories (gene ontology). This study identified highly polymorphic BES-SSRs containing tri- to hexanucleotides motifs and bringing together relevant genetic characteristics useful for breeding programs. Additionally, the BESs were incorporated into the international genome-sequencing project for the common bean.
650 $aBeans
650 $aFabaceae
650 $aGenomics
650 $aMicrosatellite repeats
650 $aMolecular genetics
650 $aTranscription factors
650 $aTransposons
650 $aFeijão
650 $aGenética molecular
650 $aPhaseolus vulgaris
700 1 $aSAKAMOTO, T.
700 1 $aMENEZES, I. P. P. de
700 1 $aPRADO, G. S.
700 1 $aMARTINS, W. S.
700 1 $aBRONDANI, C.
700 1 $aBARROS, E. G. de
700 1 $aVIANELLO, R. P.
773 $tPlant Molecular Biology, Dordrecht$gv. 86, n. 4/5, p. 455-470, Nov. 2014.
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