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| Registros recuperados : 192 | |
| 62. |  | MOREIRA, M.; CHAGAS, M.; MAGALHAES, B.; VICENTINE, S.; BARRETO, M. de F. Acao de Metarhizium flavoviride sobre Schistocerca pallens em condicoes de campo no Rio Grande do Norte. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 15., 1995, Caxambu, MG. Resumos. [S.l.]: Sociedade Entomologica do Brasil, 1995. p.410. p.410 Resumo.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 66. | | SANTOS, V. R. dos; VIANA, T. V.; BARRETO, M. B. de B.; SOUZA, F. A. de. Endomicorrizas conhecer para preservar:uma proposta de trabalho na educação ambiental. In: CONGRESSO NACIONAL DE BOTÂNICA, 64.; ENCONTRO REGIONAL DE BOTÂNICOS MG, BA E ES, 23., 2013, Belo Horizonte. Anais... Belo Horizonte: SBB, 2013.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 68. | | BARRETO, M. R.; GUIMARAES, C. T.; TEIXEIRA, F. F.; PAIVA, E.; VALICENTE, F. H. Effect of baculovirus spodoptera isolates in Spodoptera frugiperda (J. E. Smith) (Lepidoptera: Noctuidae) larvae and their characterization by RAPD. Neotropical Entomology, Londrina, v. 34, n. 1, p. 67-75, 2005.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 69. | | HAMADA, H.; BARRETO, M.; SCALOPPI, E. A. G; FRREIRA, M. R.; TOKUYAMA, R. M. Estimativas de molhamento foliar para uso em sistemas de previsao de doencas do tomateiro. Summa Phytopathologica, Piracicaba, v.27, n.1, p.97-98, jan./mar. 2001. Resumo.| Biblioteca(s): Embrapa Hortaliças. |
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| 71. | | TOKUYAMA, R. M.; BARRETO, M.; HAMADA, H.; SCALOPPI, E. A. G.; FERREIRA, M. R. Validacao de um sistema de previsao de septoriose em tomateiro. Summa Phytopathologica, Piracicaba, v.27, n.1, p.101, jan./mar. 2001. Resumo.| Biblioteca(s): Embrapa Hortaliças. |
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| 76. | | BARRETO, M. R.; QUEIROZ, D. L. de; BURCKHARDT, D.; FOERSTERI, L. A. Checklist of jumping plant-lice (Hemiptera, Sternorrhyncha, Psyllidae) from Mato Grosso, Brazil. Ciência Florestal, Santa Maria, RS, v. 30, n. 3, p. 873-884, jul./set 2020.| Biblioteca(s): Embrapa Florestas. |
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| 77. | | MARROCOS, P. C. L.; ANDERSEN, O.; BRUCKNER, C. H.; VIEIRA, G.; TAVARES, S.; BARRETO, M. Composicao quimica do oleo das amendoas de quatro cultivares de Macadamia integrifolia Maiden e Betche Revista Brasileira de Fruticultura, v.20, n.3, p.407-410, Cruz das Almas, 1998| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 78. | | CORRADINE, L.; AGUIAR, I. B.; BARRETO, M.; ARANTES, A. G. B.; CARRARA, M. A. Controle químico de "Damping-off" em sementeiras de Eucalyptus grandis Hill ex Maiden. Silvicultura, São Paulo, v. 2, n. 14, p. 101-103, 1979. Edicao especial| Biblioteca(s): Embrapa Florestas. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
|
Data corrente: |
21/10/2011 |
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Data da última atualização: |
29/09/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
ARGOLO FILHO, R. C.; GOMES JR. R. A.; BARRETO, M. R.; LANA, U. G. de P.; VALICENTE, F. H.; LOGUERCIO, L. L. |
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Afiliação: |
Ronaldo C Argôlo Filho, UESC; Rafael A. Gomes Júnior, FIOCRUZ; Marliton R. Barreto, UFMT; UBIRACI GOMES DE PAULA LANA, CNPMS; FERNANDO HERCOS VALICENTE, CNPMS; Leandro L. Loguercio, UESC. |
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Título: |
Growth variation among Bacillus thuringiensis strains can affect screening procedures for supernatant-secreted toxins against insect pests. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Pest Management Science, Sussex, v. 67, p. 1184-1192, 2011. |
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DOI: |
10.1002/ps.2206 |
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Idioma: |
Inglês |
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Conteúdo: |
BACKGROUND: Supernatant-secreted proteins in Bacillus thuringiensis (Berliner) (Bt) with insecticidal activity provide an important source of information for discovery of new useful strains and/or entomotoxins. However, physiological variation among isolates might interfere in the detection efficiency of screening procedures on Bt collections. The aim of this study was to assess the magnitude of this variation in a sample of isolates from a tropical Bt collection, which was gauged through the assessment of their temporal patterns of growth and protein secretion in culture supernatants (SNs), as well as of the corresponding toxicity against fall armyworm(Spodoptera frugiperda, JE Smith). Feedingbioassays were performed, with larvae being treated with heated and non-heated total protein extracted from SNs collected at different culture times. Larvamortality and reduction in pupa formation were observed. RESULTS: Intra- and interisolate variations were observed in the temporal patterns of growth, quality and quantity of protein secreted, aswell as in insecticidal activity of these SNs, based on larvaemortality and pupation rates. These results suggest that the insecticidal potential of certain isolates can be hidden if comparisons are done on the basis of the same number of cells in the culture and/or the same culturing time. CONCLUSIONS: Methods of screening Bt collections on the basis of feeding bioassays can be misleading with regards to identifyingmore promising isolates for biocontrol purposes if physiological differences are not considered. The consequences and implications of these findings for the development of experimental systems that depend on toxicity bioassays to identify alternative Bt strains and entomotoxins with practical applicability have been discussed. MenosBACKGROUND: Supernatant-secreted proteins in Bacillus thuringiensis (Berliner) (Bt) with insecticidal activity provide an important source of information for discovery of new useful strains and/or entomotoxins. However, physiological variation among isolates might interfere in the detection efficiency of screening procedures on Bt collections. The aim of this study was to assess the magnitude of this variation in a sample of isolates from a tropical Bt collection, which was gauged through the assessment of their temporal patterns of growth and protein secretion in culture supernatants (SNs), as well as of the corresponding toxicity against fall armyworm(Spodoptera frugiperda, JE Smith). Feedingbioassays were performed, with larvae being treated with heated and non-heated total protein extracted from SNs collected at different culture times. Larvamortality and reduction in pupa formation were observed. RESULTS: Intra- and interisolate variations were observed in the temporal patterns of growth, quality and quantity of protein secreted, aswell as in insecticidal activity of these SNs, based on larvaemortality and pupation rates. These results suggest that the insecticidal potential of certain isolates can be hidden if comparisons are done on the basis of the same number of cells in the culture and/or the same culturing time. CONCLUSIONS: Methods of screening Bt collections on the basis of feeding bioassays can be misleading with regards to identifyingmore promising isolates fo... Mostrar Tudo |
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Thesagro: |
Controle biológico; Spodoptera Frugiperda. |
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Thesaurus NAL: |
Biological control. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 02561naa a2200229 a 4500
001 1903801
005 2017-09-29
008 2011 bl uuuu u00u1 u #d
024 7 $a10.1002/ps.2206$2DOI
100 1 $aARGOLO FILHO, R. C.
245 $aGrowth variation among Bacillus thuringiensis strains can affect screening procedures for supernatant-secreted toxins against insect pests.$h[electronic resource]
260 $c2011
520 $aBACKGROUND: Supernatant-secreted proteins in Bacillus thuringiensis (Berliner) (Bt) with insecticidal activity provide an important source of information for discovery of new useful strains and/or entomotoxins. However, physiological variation among isolates might interfere in the detection efficiency of screening procedures on Bt collections. The aim of this study was to assess the magnitude of this variation in a sample of isolates from a tropical Bt collection, which was gauged through the assessment of their temporal patterns of growth and protein secretion in culture supernatants (SNs), as well as of the corresponding toxicity against fall armyworm(Spodoptera frugiperda, JE Smith). Feedingbioassays were performed, with larvae being treated with heated and non-heated total protein extracted from SNs collected at different culture times. Larvamortality and reduction in pupa formation were observed. RESULTS: Intra- and interisolate variations were observed in the temporal patterns of growth, quality and quantity of protein secreted, aswell as in insecticidal activity of these SNs, based on larvaemortality and pupation rates. These results suggest that the insecticidal potential of certain isolates can be hidden if comparisons are done on the basis of the same number of cells in the culture and/or the same culturing time. CONCLUSIONS: Methods of screening Bt collections on the basis of feeding bioassays can be misleading with regards to identifyingmore promising isolates for biocontrol purposes if physiological differences are not considered. The consequences and implications of these findings for the development of experimental systems that depend on toxicity bioassays to identify alternative Bt strains and entomotoxins with practical applicability have been discussed.
650 $aBiological control
650 $aControle biológico
650 $aSpodoptera Frugiperda
700 1 $aGOMES JR. R. A.
700 1 $aBARRETO, M. R.
700 1 $aLANA, U. G. de P.
700 1 $aVALICENTE, F. H.
700 1 $aLOGUERCIO, L. L.
773 $tPest Management Science, Sussex$gv. 67, p. 1184-1192, 2011.
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