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Registros recuperados : 192 | |
83. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | MOREIRA, M. A.; MAGALHAES, B. P.; VICENTINI, S.; BARRETO, M. F. P.; FRAZAO, H. S. Efeito do fungo Metarhizium flavoviride sobre Stiphra robusta (Orthoptera: Proscopiidae) em condicoes de campo. In: SIMPOSIO DE CONTROLE BIOLOGICO, 5., 1996, Foz do Iguacu, PR. Anais: sessao de posters. Londrina: EMBRAPA-CNPSO, 1996. p.265. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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85. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | BARRETO, M. R.; TEIXEIRA, F. F.; LOGUERCIO, L. L.; VALICENTE, F. H.; PAIVA, E. Efeito de isolados de Baculovirus em lagartas de Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) e sua caracterização utilizando PCR. Genetics and Molecular Biology, Ribeirão Preto, v. 22, n. 3, p. 430, 1999. Suplemento. Edição dos resumos do Congresso Nacional de Genética, Gramado, 1999. Biblioteca(s): Embrapa Milho e Sorgo. |
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88. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | BOARI, A. de J.; PANTOJA, K. F. C.; SOUSA, C. M.; BARRETO, M.; SECUNDINO, W. Avaliação de jardins clonais de pimenta-do-reino quanto à presença de Piper yellow mottle virus no Estado do Espírito Santo. In: CONGRESSO PAULISTA DE FITOPATOLOGIA, 35., 2012, Jaguariúna. Anais... Summa Phytopathologica, Botucatu, v. 38, fev. 2012. Suplemento. 1 CD-ROM. Biblioteca(s): Embrapa Amazônia Oriental. |
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91. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | BARRETO, M. A.; ALMEIDA, M. A. O. de; SILVA, A.; BITENCUR, C. P. Avaliação da resistência anti-helmíntica de nematódeos gastrintestinais em ovinos deslanados do estado da Bahia. Revista Brasileira de Parasitologia Veterinária, v. 13, p. 265, 2004. Suplemento 1. Resumo 229. Edição de anais do 13o. Congresso Brasileiro de Parasitologia Veterinária e o 1o. Simpósio Latino-Americano de Rickettsioses, Ouro Preto, MG, set. 2004. Biblioteca(s): Embrapa Caprinos e Ovinos. |
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94. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | LOGUERCIO, L. L.; SANTOS, C. G.; BARRETO, M. R.; GUIMARAES, C. T.; PAIVA, E. Association of PCR and feeding bioassays as a large-scale method to screen tropical Bacillus thuringiensis isolates for a cry constitution with higher insecticidal effect against Spodoptera frugiperda (Lepidoptera: Noctuidae) larvae. Letters in Applied Microbiology, Oxford, v. 32, n. 5, p. 362-367, 2001. Biblioteca(s): Embrapa Milho e Sorgo. |
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97. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | MARROCOS, P. C. L.; ANDERSEN, O.; BRUCKNER, C. H.; VIEIRA, G.; TAVARES, S.; BARRETO, M. Composicao quimica do oleo das amendoas de quatro cultivares de Macadamia integrifolia Maiden e Betche Revista Brasileira de Fruticultura, v.20, n.3, p.407-410, Cruz das Almas, 1998 Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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98. | ![Imagem marcado/desmarcado](/consulta/web/img/desmarcado.png) | LOGUERCIO, L. L.; BARRETO, M. R.; SANTOS, C. G. dos; VALICENTE, F. H.; PAIVA, E. Padrão temporal de secreção de proteínas e atividade inseticida contra Spodoptera frugiperda (J. E. Smith) (Lepdoptera: noctuidae), no sobrenadante da cepa de Bacillus thuringiensis (Berlinger) 'HD125'. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 20., 1999, Salvador. Resumos... Salvador: SBM, 1999. Biblioteca(s): Embrapa Milho e Sorgo. |
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Registros recuperados : 192 | |
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Registro Completo
Biblioteca(s): |
Embrapa Amapá. |
Data corrente: |
29/11/2022 |
Data da última atualização: |
29/11/2022 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
CAVALCANTE, M. de A.; OLIVEIRA, J. dos S.; BARRETO, M. S. da S.; PINHEIRO, L. P.; CANTUÁRIA, P. de C.; BORGES, W. L.; SILVA, G. A. da; SOUZA, T. M. de. |
Afiliação: |
MARÍLIA DE A. CAVALCANTE, INSTITUTO FEDERAL DO AMAPÁ; JANYNA DOS S. OLIVEIRA, INSTITUTO FEDERAL DO AMAPÁ; MAYRA S. DA S. BARRETO, UNIVERSIDADE DO ESTADO DO AMAPÁ; LUCAS P. PINHEIRO, UNIVERSIDADE DO ESTADO DO AMAPÁ; PATRICK DE C. CANTUÁRIA, INSTITUTO DE PESQUISAS CIENTÍFICAS E TECNOLÓGICAS DO ESTADO DO AMAPÁ; WARDSSON LUSTRINO BORGES, CPAF-AP; GABRIEL A. DA SILVA, UNIVERSIDADE DO ESTADO DO AMAPÁ; TIAGO MARCOLINO DE SOUZA, UNIVERSIDADE DO ESTADO DO AMAPÁ. |
Título: |
An HPLC method to determine phenolic compounds of plant extracts: application to Byrsonima crassifolia and Senna alata leaves. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
Pharmacognosy Research, v. 14, n. 4, p. 395-404, 2022. |
DOI: |
10.5530/pres.14.4.58 |
Idioma: |
Inglês |
Conteúdo: |
Background: The Amazonian Region has a variety of medicinal plants with bioactive compounds, whose characterization could present the potential for sustainable development. Objectives: A method for separating, identifying, and quantifying a mixture of nine phenolic compounds (gallic acid, 3-hydroxybenzoic acid, p-coumaric acid, catechin, myricetin, rutin, quercetin, kaempferol, and cyanidin) was developed, validated, and applied to analyze aqueous and hydroethanolic extracts from Byrsonima crassifolia (L.) Kunth and Senna alata (L.) leaves. Materials and Methods: The separation was carried out by HPLC, using a Shim-pack VP-ODS C18 column (5 μm, 150 x 4.6 mm) at 40°C. Detection was performed at 254 nm and separation occurred in 35 min. Results: The optimized method was validated for each of the nine phenolic compounds. The calibration curve for the phenolic compound standards showed suitable linear fitting and exhibited correlation coefficients greater than 0.990. The LOD and LOQ varied between 6.2807 - 14.8851 μg mL-1 and 6.8002 - 16.0071 μg mL-1, respectively. The method was found to be robust for changes of ±2 ml in mobile phase composition. Byrsonima crassifolia aqueous extracts indicated contents of gallic acid, catechin, rutin, and cyanidin whereas hydroethanolic one did not show the first substance. Senna alata aqueous extract presented only 3-hydroxybenzoic acid and rutin whereas myricetin, cyanidin, quercetin, and kaempferol were also identified in the hydroethanolic one. Conclusion: The HPLC method is efficient, precise, accurate, and sensitive to determining phenolic compounds in plant extracts and it is recommended for efficient assays in routine work. MenosBackground: The Amazonian Region has a variety of medicinal plants with bioactive compounds, whose characterization could present the potential for sustainable development. Objectives: A method for separating, identifying, and quantifying a mixture of nine phenolic compounds (gallic acid, 3-hydroxybenzoic acid, p-coumaric acid, catechin, myricetin, rutin, quercetin, kaempferol, and cyanidin) was developed, validated, and applied to analyze aqueous and hydroethanolic extracts from Byrsonima crassifolia (L.) Kunth and Senna alata (L.) leaves. Materials and Methods: The separation was carried out by HPLC, using a Shim-pack VP-ODS C18 column (5 μm, 150 x 4.6 mm) at 40°C. Detection was performed at 254 nm and separation occurred in 35 min. Results: The optimized method was validated for each of the nine phenolic compounds. The calibration curve for the phenolic compound standards showed suitable linear fitting and exhibited correlation coefficients greater than 0.990. The LOD and LOQ varied between 6.2807 - 14.8851 μg mL-1 and 6.8002 - 16.0071 μg mL-1, respectively. The method was found to be robust for changes of ±2 ml in mobile phase composition. Byrsonima crassifolia aqueous extracts indicated contents of gallic acid, catechin, rutin, and cyanidin whereas hydroethanolic one did not show the first substance. Senna alata aqueous extract presented only 3-hydroxybenzoic acid and rutin whereas myricetin, cyanidin, quercetin, and kaempferol were also identified in the... Mostrar Tudo |
Palavras-Chave: |
Compostos bioativos; Método cromatográfico. |
Thesagro: |
Cromatografia; Extrato Vegetal. |
Thesaurus NAL: |
Bioactive compounds; Plant extracts. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1148941/1/CPAF-AP-AnHPLCMethod.pdf
|
Marc: |
LEADER 02625naa a2200289 a 4500 001 2148941 005 2022-11-29 008 2022 bl uuuu u00u1 u #d 024 7 $a10.5530/pres.14.4.58$2DOI 100 1 $aCAVALCANTE, M. de A. 245 $aAn HPLC method to determine phenolic compounds of plant extracts$bapplication to Byrsonima crassifolia and Senna alata leaves.$h[electronic resource] 260 $c2022 520 $aBackground: The Amazonian Region has a variety of medicinal plants with bioactive compounds, whose characterization could present the potential for sustainable development. Objectives: A method for separating, identifying, and quantifying a mixture of nine phenolic compounds (gallic acid, 3-hydroxybenzoic acid, p-coumaric acid, catechin, myricetin, rutin, quercetin, kaempferol, and cyanidin) was developed, validated, and applied to analyze aqueous and hydroethanolic extracts from Byrsonima crassifolia (L.) Kunth and Senna alata (L.) leaves. Materials and Methods: The separation was carried out by HPLC, using a Shim-pack VP-ODS C18 column (5 μm, 150 x 4.6 mm) at 40°C. Detection was performed at 254 nm and separation occurred in 35 min. Results: The optimized method was validated for each of the nine phenolic compounds. The calibration curve for the phenolic compound standards showed suitable linear fitting and exhibited correlation coefficients greater than 0.990. The LOD and LOQ varied between 6.2807 - 14.8851 μg mL-1 and 6.8002 - 16.0071 μg mL-1, respectively. The method was found to be robust for changes of ±2 ml in mobile phase composition. Byrsonima crassifolia aqueous extracts indicated contents of gallic acid, catechin, rutin, and cyanidin whereas hydroethanolic one did not show the first substance. Senna alata aqueous extract presented only 3-hydroxybenzoic acid and rutin whereas myricetin, cyanidin, quercetin, and kaempferol were also identified in the hydroethanolic one. Conclusion: The HPLC method is efficient, precise, accurate, and sensitive to determining phenolic compounds in plant extracts and it is recommended for efficient assays in routine work. 650 $aBioactive compounds 650 $aPlant extracts 650 $aCromatografia 650 $aExtrato Vegetal 653 $aCompostos bioativos 653 $aMétodo cromatográfico 700 1 $aOLIVEIRA, J. dos S. 700 1 $aBARRETO, M. S. da S. 700 1 $aPINHEIRO, L. P. 700 1 $aCANTUÁRIA, P. de C. 700 1 $aBORGES, W. L. 700 1 $aSILVA, G. A. da 700 1 $aSOUZA, T. M. de 773 $tPharmacognosy Research$gv. 14, n. 4, p. 395-404, 2022.
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