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Registro Completo |
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
22/02/2013 |
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Data da última atualização: |
07/03/2023 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
CARVALHO, J. O.; MICHALCZECHEN-LACERDA, V. A.; SARTORI, R.; RODRIGUES, F. C.; BRAVIM, O.; FRANCO, M. M.; DODE, M. A. N. |
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Afiliação: |
JOSE O. CARVALHO, UNIVERSIDADE DE SÃO PAULO; VALQUIRIA A. MICHALCZECHEN-LACERDA, UnB; ROBERTO SARTORI, UNIVERSIDADE DE SÃO PAULO; FERNANDA C. RODRIGUES; OTAVIO BRAVIM, UnB; MAURICIO MACHAIM FRANCO, CENARGEN; MARGOT ALVES NUNES DODE, CENARGEN. |
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Título: |
The methylation patterns of the IGF2 and IGF2R genes in bovine spermatozoa are not affected by flow-cytometric sex sorting. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Molecular Reproduction e Development, v. 79, p. 77-84, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
The objectives of this study were to investigate the effect of sexing by flow cytometry on the methylation patterns of the IGF2 and IGF2R genes. Frozen-thawed, unsorted, and sex-sorted spermsamples fromfour Nellore bulls were used. Each ejaculate was separated into three fractions: non-sexed (NS), sexed forX-sperm(SX), and sexed for Y-sperm (SY). Sperm were isolated from the extender, cryoprotectant, and other cell types by centrifugation on a 40:70%Percoll gradient, and spermpellets were used for genomic DNA isolation. DNA was used for analyses of the methylation patterns by bisul?te sequencing.Methylation status of the IGF2 and IGF2Rgeneswere evaluated by sequencing 195 and 147 individual clones, respectively. No global differences in DNAmethylation were found between NS, SX, and SY groups for the IGF2 (P¼0.09) or IGF2R genes (P¼0.38). Very speci?c methylation patterns were observed in the 25th and 26th CpG sites in the IGF2R gene. representing higher methylation in NS than in the SX and SY groups compared with the other CpG sites. Further, individual variation in methylation patterns was found among bulls. In conclusion, the sex-sorting procedure by flow cytometry did not affect the overall DNA methylation patterns of the IGF2 and IGF2R genes, although individual variation in their methyla-tion patterns among bulls was observed. |
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Palavras-Chave: |
Gene IGF2; Gene IGF2R; Sexagem por citometria. |
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Thesagro: |
Bovino. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/179337/1/Carvalho-et-al-2012-Molecular-Reproduction-and-Development.pdf
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Marc: |
LEADER 02081nam a2200229 a 4500
001 1950725
005 2023-03-07
008 2012 bl uuuu u00u1 u #d
100 1 $aCARVALHO, J. O.
245 $aThe methylation patterns of the IGF2 and IGF2R genes in bovine spermatozoa are not affected by flow-cytometric sex sorting.$h[electronic resource]
260 $aMolecular Reproduction e Development, v. 79, p. 77-84, 2012.$c2012
520 $aThe objectives of this study were to investigate the effect of sexing by flow cytometry on the methylation patterns of the IGF2 and IGF2R genes. Frozen-thawed, unsorted, and sex-sorted spermsamples fromfour Nellore bulls were used. Each ejaculate was separated into three fractions: non-sexed (NS), sexed forX-sperm(SX), and sexed for Y-sperm (SY). Sperm were isolated from the extender, cryoprotectant, and other cell types by centrifugation on a 40:70%Percoll gradient, and spermpellets were used for genomic DNA isolation. DNA was used for analyses of the methylation patterns by bisul?te sequencing.Methylation status of the IGF2 and IGF2Rgeneswere evaluated by sequencing 195 and 147 individual clones, respectively. No global differences in DNAmethylation were found between NS, SX, and SY groups for the IGF2 (P¼0.09) or IGF2R genes (P¼0.38). Very speci?c methylation patterns were observed in the 25th and 26th CpG sites in the IGF2R gene. representing higher methylation in NS than in the SX and SY groups compared with the other CpG sites. Further, individual variation in methylation patterns was found among bulls. In conclusion, the sex-sorting procedure by flow cytometry did not affect the overall DNA methylation patterns of the IGF2 and IGF2R genes, although individual variation in their methyla-tion patterns among bulls was observed.
650 $aBovino
653 $aGene IGF2
653 $aGene IGF2R
653 $aSexagem por citometria
700 1 $aMICHALCZECHEN-LACERDA, V. A.
700 1 $aSARTORI, R.
700 1 $aRODRIGUES, F. C.
700 1 $aBRAVIM, O.
700 1 $aFRANCO, M. M.
700 1 $aDODE, M. A. N.
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Registro original: |
Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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Registro Completo
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Biblioteca(s): |
Embrapa Instrumentação; Embrapa Pecuária Sudeste. |
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Data corrente: |
13/01/2015 |
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Data da última atualização: |
13/01/2015 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
BERNARDI, A. C. C.; BARIONI-JUNIOR, W.; INAMASU, R. Y. |
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Afiliação: |
RICARDO YASSUSHI INAMASU, CNPDIA. |
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Título: |
Análise de correspondência múltipla para caracterização do perfil de adotantes de agricultura de precisão no Brasil. |
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Ano de publicação: |
2014 |
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Fonte/Imprenta: |
In: SIMPÓSIO NACIONAL DE INSTRUMENTAÇÃO AGROPECUÁRIA, 2014, São Carlos, SP Anais do SIAGRO: ciência, inovação e mercado 2014. São Carlos, SP: Embrapa Instrumentação, 2014. p. 73-76. Editores: Carlos Manoel Pedro Vaz, Débora Marcondes Bastos Pereira Milori, Silvio Crestana. |
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Idioma: |
Português |
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Palavras-Chave: |
Agricultores; Análise de correspondência múltipla; Fatores de adoção; Ferramentas de AP; Instrumentação - Evento; Qui quadrado. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/115344/1/73siagro-2014-print01.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1154292/1/AnaliseCorrespondenciaMultipla.pdf
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Marc: |
LEADER 00903nam a2200181 a 4500
001 2005297
005 2015-01-13
008 2014 bl uuuu u00u1 u #d
100 1 $aBERNARDI, A. C. C.; BARIONI-JUNIOR, W.
245 $aAnálise de correspondência múltipla para caracterização do perfil de adotantes de agricultura de precisão no Brasil.$h[electronic resource]
260 $aIn: SIMPÓSIO NACIONAL DE INSTRUMENTAÇÃO AGROPECUÁRIA, 2014, São Carlos, SP Anais do SIAGRO: ciência, inovação e mercado 2014. São Carlos, SP: Embrapa Instrumentação, 2014. p. 73-76. Editores: Carlos Manoel Pedro Vaz, Débora Marcondes Bastos Pereira Milori, Silvio Crestana.$c2014
653 $aAgricultores
653 $aAnálise de correspondência múltipla
653 $aFatores de adoção
653 $aFerramentas de AP
653 $aInstrumentação - Evento
653 $aQui quadrado
700 1 $aINAMASU, R. Y.
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Embrapa Instrumentação (CNPDIA) |
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