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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
14/02/2017 |
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Data da última atualização: |
30/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
NANI, T. F.; PEREIRA, D. L.; SOUZA SOBRINHO, F. de; TECHIO, V. H. |
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Afiliação: |
Thaís Furtado Nani, UFLA; Daniele Laís Pereira, UFLA; FAUSTO DE SOUZA SOBRINHO, CNPGL; Vânia Helena Techio, UFLA. |
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Título: |
Physical map of repetitive DNA sites in Brachiaria spp.: intravarietal and interspecific polymorphisms. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Crop Science, v. 56, n. 4, p. 1769-1783, 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Brachiaria (Trin.) Griseb. is a genus belonging to the Poaceae Barnh. family that aggregates species of forage interest, which have shown different levels of ploidy, intraspecific variability for chromosomal morphology and for number of rDNA sites. This study aimed at characterizing the karyotype of Brachiaria brizantha (Hochst. ex A. Rich) Stapf, Brachiaria decumbens Stapf, and Brachiaria ruziziensis R. Germ. & C.M. Evrard by the location of 5S rDNA, 45S rDNA, centromeric retrotransposons, chromomycin A3 (CMA) and 4¢, 6-diamidino-2-phenylindole dihydrochloride (DAPI) chromosome bands, and transcriptional activity at nucleolar organizing regions (NORs). Brachiaria ruziziensis has 2n = 2x = 18 chromosomes, karyotypic formula 9M, one 45S rDNA site, and two 5S rDNA sites. Brachiaria brizantha has 2n = 4x = 36 chromosomes, karyotypic formula 18M, four to six 5S rDNA signals, and two to four 45S rDNA sites. One of the chromosomes carrying 45S rDNA is heteromorphic. Brachiaria decumbens has 2n = 4x = 36 chromosomes, karyotypic formula 17M+1M/SM, and seven 5S rDNA signals including one hemizygous 5S rDNA locus. One of the two chromosome pairs carrying 45S rDNA signals was heteromorphic with one of its chromosomes possessing a tertiary constriction. Hybridization signals with a centromeric retrotransposon probe, CRM1, were observed in Brachiaria chromosomes with distinct clusters on all B. ruziziensis chromosomes. Chromosomal CMA bands were located along with 45S rDNA signals in B. decumbens and B. ruziziensis whereas in B. brizantha only two out of four signals coincided. The karyotype of Brachiaria species is symmetrical. The transcriptional activity of NORs is variable with two or three heteromorphic nucleoli. MenosAbstract Brachiaria (Trin.) Griseb. is a genus belonging to the Poaceae Barnh. family that aggregates species of forage interest, which have shown different levels of ploidy, intraspecific variability for chromosomal morphology and for number of rDNA sites. This study aimed at characterizing the karyotype of Brachiaria brizantha (Hochst. ex A. Rich) Stapf, Brachiaria decumbens Stapf, and Brachiaria ruziziensis R. Germ. & C.M. Evrard by the location of 5S rDNA, 45S rDNA, centromeric retrotransposons, chromomycin A3 (CMA) and 4¢, 6-diamidino-2-phenylindole dihydrochloride (DAPI) chromosome bands, and transcriptional activity at nucleolar organizing regions (NORs). Brachiaria ruziziensis has 2n = 2x = 18 chromosomes, karyotypic formula 9M, one 45S rDNA site, and two 5S rDNA sites. Brachiaria brizantha has 2n = 4x = 36 chromosomes, karyotypic formula 18M, four to six 5S rDNA signals, and two to four 45S rDNA sites. One of the chromosomes carrying 45S rDNA is heteromorphic. Brachiaria decumbens has 2n = 4x = 36 chromosomes, karyotypic formula 17M+1M/SM, and seven 5S rDNA signals including one hemizygous 5S rDNA locus. One of the two chromosome pairs carrying 45S rDNA signals was heteromorphic with one of its chromosomes possessing a tertiary constriction. Hybridization signals with a centromeric retrotransposon probe, CRM1, were observed in Brachiaria chromosomes with distinct clusters on all B. ruziziensis chromosomes. Chromosomal CMA bands were located along with 45S rDNA signa... Mostrar Tudo |
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Palavras-Chave: |
CRM1; Cytogenetic analyses; Intravarietal variations; Karyotypes of Brachiaria spp. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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Marc: |
LEADER 02381naa a2200205 a 4500
001 2063953
005 2023-01-30
008 2016 bl uuuu u00u1 u #d
100 1 $aNANI, T. F.
245 $aPhysical map of repetitive DNA sites in Brachiaria spp.$bintravarietal and interspecific polymorphisms.$h[electronic resource]
260 $c2016
520 $aAbstract Brachiaria (Trin.) Griseb. is a genus belonging to the Poaceae Barnh. family that aggregates species of forage interest, which have shown different levels of ploidy, intraspecific variability for chromosomal morphology and for number of rDNA sites. This study aimed at characterizing the karyotype of Brachiaria brizantha (Hochst. ex A. Rich) Stapf, Brachiaria decumbens Stapf, and Brachiaria ruziziensis R. Germ. & C.M. Evrard by the location of 5S rDNA, 45S rDNA, centromeric retrotransposons, chromomycin A3 (CMA) and 4¢, 6-diamidino-2-phenylindole dihydrochloride (DAPI) chromosome bands, and transcriptional activity at nucleolar organizing regions (NORs). Brachiaria ruziziensis has 2n = 2x = 18 chromosomes, karyotypic formula 9M, one 45S rDNA site, and two 5S rDNA sites. Brachiaria brizantha has 2n = 4x = 36 chromosomes, karyotypic formula 18M, four to six 5S rDNA signals, and two to four 45S rDNA sites. One of the chromosomes carrying 45S rDNA is heteromorphic. Brachiaria decumbens has 2n = 4x = 36 chromosomes, karyotypic formula 17M+1M/SM, and seven 5S rDNA signals including one hemizygous 5S rDNA locus. One of the two chromosome pairs carrying 45S rDNA signals was heteromorphic with one of its chromosomes possessing a tertiary constriction. Hybridization signals with a centromeric retrotransposon probe, CRM1, were observed in Brachiaria chromosomes with distinct clusters on all B. ruziziensis chromosomes. Chromosomal CMA bands were located along with 45S rDNA signals in B. decumbens and B. ruziziensis whereas in B. brizantha only two out of four signals coincided. The karyotype of Brachiaria species is symmetrical. The transcriptional activity of NORs is variable with two or three heteromorphic nucleoli.
653 $aCRM1
653 $aCytogenetic analyses
653 $aIntravarietal variations
653 $aKaryotypes of Brachiaria spp
700 1 $aPEREIRA, D. L.
700 1 $aSOUZA SOBRINHO, F. de
700 1 $aTECHIO, V. H.
773 $tCrop Science$gv. 56, n. 4, p. 1769-1783, 2016.
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Embrapa Gado de Leite (CNPGL) |
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
28/09/1998 |
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Data da última atualização: |
04/02/2019 |
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Autoria: |
BARCELOS, E. |
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Afiliação: |
Embrapa Amazônia Ocidental. |
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Título: |
Etude de la diversité génétique du genre Elaeis (E. oleifera (Kunth) Cortés et E. guineensis Jacq.) par marqueurs moléculaires (RFLP et AFLP). |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
1998. |
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Páginas: |
136 f. il. |
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Idioma: |
Francês |
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Notas: |
Thèse (Diplôme de Doctorat) - Ecole Nationale Supérieure Agronomique de Montpellier, Montpellier. |
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Conteúdo: |
Genetic diversity and its organization within oil palm E. oleifera and E. guineensis germplasm were evaluated by using molecular markers. Restriction fragment length polymorphism RFLP genetic variability was revealed with 37 nuclear cDNA, 1 rDNA, 2 chloroplastic and 4 mitochondrial probes over 241 accessions of the American species E. oleifera from Brazil, Peru, North of Colombia, Central America, French Guyana and Surinam and 38 accessions of African species E. guineensis from Angola, Cote d'Ivoire, Cameroun, Zaire, Benin, Nigeria, and Deli and Brazil/Bahia introduced origins. These accessions cover a large part of the geographical distribution areas of these species on the American and African continents, respectively. Amplified fragment length polymorphism AFLP technique was performed on a sub-set of 44 accessions of E. oleifera and 22 accessions of E. guineensis by using three pairs of enzyme/primer combinations. Nuclear DNA content variation was assessed by flow cytometry. Data were subjected to factorial analysis of correspondence, cluster analysis and population F-statistics genetic analysis. Results appeared very congruent between RFLP and AFLP, with both type of markers revealing that genetic divergence between the two species is surprisingly of the same magnitude as in the E. oleifera species alone, this finding being in discrepancy with the early Tertiary separation of the two species. At the E. oleifera species level, a strong structuring of genetic diversity was revealed according to the geographical origin of the studied material, with the identification of four distinct genetic groups, Brazil, Peru, Central America and French Guyana/Surinam, with 74% of total variability being due to the difference among these groups. The genetic structuring revealed within E. oleifera origins from Brazilian Amazon region is in agreement with the fluvial network of this region, with 35% of the overall variability within 32 studied populations being due to difference between these populations. The Elaeis cytoplasmic genome revealed a low genetic diversity with only two types of chloroplast for the whole genera, one of this types being common to the two species while the other type eas specific to the E. oleifera accessions originated from Central Amarica. The mitochondrial genome analysis revealed six mitochondrial types in the American species and two in the African species (4.03+-0.11 pg) with accessions from Surinam presenting the lower quantity. The African species presents the same DNA quanty as Surinam accessions. Within this genus, genetic diversity organization and evolution was discussed and consequences on oil palm genetics resources management and utilization were addressed. MenosGenetic diversity and its organization within oil palm E. oleifera and E. guineensis germplasm were evaluated by using molecular markers. Restriction fragment length polymorphism RFLP genetic variability was revealed with 37 nuclear cDNA, 1 rDNA, 2 chloroplastic and 4 mitochondrial probes over 241 accessions of the American species E. oleifera from Brazil, Peru, North of Colombia, Central America, French Guyana and Surinam and 38 accessions of African species E. guineensis from Angola, Cote d'Ivoire, Cameroun, Zaire, Benin, Nigeria, and Deli and Brazil/Bahia introduced origins. These accessions cover a large part of the geographical distribution areas of these species on the American and African continents, respectively. Amplified fragment length polymorphism AFLP technique was performed on a sub-set of 44 accessions of E. oleifera and 22 accessions of E. guineensis by using three pairs of enzyme/primer combinations. Nuclear DNA content variation was assessed by flow cytometry. Data were subjected to factorial analysis of correspondence, cluster analysis and population F-statistics genetic analysis. Results appeared very congruent between RFLP and AFLP, with both type of markers revealing that genetic divergence between the two species is surprisingly of the same magnitude as in the E. oleifera species alone, this finding being in discrepancy with the early Tertiary separation of the two species. At the E. oleifera species level, a strong structuring of genetic diversity was... Mostrar Tudo |
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Palavras-Chave: |
Isoenzymes; Plant population. |
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Thesagro: |
Dendê; Elaeis Guineensis; Elaeis Oleifera; Isoenzima; População de Planta; Variação Genética. |
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Thesaurus NAL: |
genetic variation. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/35291/1/TsEdson001.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/35308/1/TsEdson002.pdf
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Marc: |
LEADER 03519nam a2200241 a 4500
001 1666838
005 2019-02-04
008 1998 bl uuuu m 00u1 u #d
100 1 $aBARCELOS, E.
245 $aEtude de la diversité génétique du genre Elaeis (E. oleifera (Kunth) Cortés et E. guineensis Jacq.) par marqueurs moléculaires (RFLP et AFLP).
260 $a1998.$c1998
300 $a136 f. il.
500 $aThèse (Diplôme de Doctorat) - Ecole Nationale Supérieure Agronomique de Montpellier, Montpellier.
520 $aGenetic diversity and its organization within oil palm E. oleifera and E. guineensis germplasm were evaluated by using molecular markers. Restriction fragment length polymorphism RFLP genetic variability was revealed with 37 nuclear cDNA, 1 rDNA, 2 chloroplastic and 4 mitochondrial probes over 241 accessions of the American species E. oleifera from Brazil, Peru, North of Colombia, Central America, French Guyana and Surinam and 38 accessions of African species E. guineensis from Angola, Cote d'Ivoire, Cameroun, Zaire, Benin, Nigeria, and Deli and Brazil/Bahia introduced origins. These accessions cover a large part of the geographical distribution areas of these species on the American and African continents, respectively. Amplified fragment length polymorphism AFLP technique was performed on a sub-set of 44 accessions of E. oleifera and 22 accessions of E. guineensis by using three pairs of enzyme/primer combinations. Nuclear DNA content variation was assessed by flow cytometry. Data were subjected to factorial analysis of correspondence, cluster analysis and population F-statistics genetic analysis. Results appeared very congruent between RFLP and AFLP, with both type of markers revealing that genetic divergence between the two species is surprisingly of the same magnitude as in the E. oleifera species alone, this finding being in discrepancy with the early Tertiary separation of the two species. At the E. oleifera species level, a strong structuring of genetic diversity was revealed according to the geographical origin of the studied material, with the identification of four distinct genetic groups, Brazil, Peru, Central America and French Guyana/Surinam, with 74% of total variability being due to the difference among these groups. The genetic structuring revealed within E. oleifera origins from Brazilian Amazon region is in agreement with the fluvial network of this region, with 35% of the overall variability within 32 studied populations being due to difference between these populations. The Elaeis cytoplasmic genome revealed a low genetic diversity with only two types of chloroplast for the whole genera, one of this types being common to the two species while the other type eas specific to the E. oleifera accessions originated from Central Amarica. The mitochondrial genome analysis revealed six mitochondrial types in the American species and two in the African species (4.03+-0.11 pg) with accessions from Surinam presenting the lower quantity. The African species presents the same DNA quanty as Surinam accessions. Within this genus, genetic diversity organization and evolution was discussed and consequences on oil palm genetics resources management and utilization were addressed.
650 $agenetic variation
650 $aDendê
650 $aElaeis Guineensis
650 $aElaeis Oleifera
650 $aIsoenzima
650 $aPopulação de Planta
650 $aVariação Genética
653 $aIsoenzymes
653 $aPlant population
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