Data/hora: 23/06/2024 04:27:37

Biblioteca(s): | Embrapa Soja.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | A - 1
Data corrente: | 19/03/2010
Data da última atualização: | 19/01/2017
Autoria: | OLIVEIRA, L. R.; MARCELINO, F. C.; BARCELLOS, F. G.; RODRIGUES, E. P.; MEGIAS, M.; HUNGRIA, M.
Afiliação: | LUCIANA RUANO OLIVEIRA, UEL - CNPq; FRANCISMAR CORREA MARCELINO, CNPSo; FERNANDO GOMES BARCELLOS, CNPSo; ELISETE PAINS RODRIGUES, CNPSo - CNPq; MANUEL MEGIAS, Universidad de Sevilha; MARIANGELA HUNGRIA DA CUNHA, CNPSo.
Título: | The nodC, nodG, and glgX genes of Rhizobium tropici strain PRF 81.
Ano de publicação: | 2010
Fonte/Imprenta: | Functional & Integrative Genomics, Heidelberg, v. 10, n. 3, p. 425-431, Aug. 2010.
DOI: | DOI 10.1007/s10142-009-0151-x
Idioma: | Inglês
Conteúdo: | Rhizobium tropici is a diazotrophic microsymbiont of common bean (Phaseolus vulgaris L.) that encompasses important but still poorly studied tropical strains, and a recent significant contribution to the knowledge of the species was the publication of a genomic draft of strain PRF 81, which revealed several novel genes [Pinto et al. Funct Int Gen 9:263?270, 2009]. In this study, we investigated the transcription of nodC, nodG, and glgX genes, located in the nod operon of PRF 81 strain, by reverse-transcription quantitative PCR. All three genes showed low levels of transcription when the cells were grown until exponential growth phase in the presence of common-bean-seed exudates or of the root nod-gene inducer naringenin. However, when cells at the exponential phase of growth were incubated with seed exudates, transcription occurred after only 5 min, and nodC, nodG, and glgX were transcribed 121.97-, 14.86-, and 50.29-fold more than the control, respectively, followed by a rapid decrease in gene transcription. Much lower levels of transcription were observed in the presence of naringenin; furthermore, maximum transcription required 8 h of incubation for all three genes. In light of these results, the mechanisms of induction of the nodulation genes by flavonoids are discussed.
Palavras-Chave: | Reverse transcription quantitative-PCR.
Thesagro: | Feijao; Fixacao de nitrogenio; Gene; Phaseolus Vulgaris; Simbiose.
Thesaurus Nal: | Beans; Genes; Nitrogen fixation; Rhizobium tropici; Symbiosis.
Categoria do assunto: | G Melhoramento Genético
Registro original: | Embrapa Soja (CNPSO)
Exemplares: | CNPSO - (30608-1) - UPC - AP - PP - 11785 -  - 11785 - DIS