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| Registros recuperados : 5 | |
| 2. |  | GUEDES JUNIOR, D. S.; ARAUJO, F. R.; SILVA, F. J. M.; RANGEL, C. P.; BARBOSA NETO, J. D.; FONSECA, A. H. Frequency of antibodies to Babesia bigemina, B. bovis, Anaplasma marginale, Trypanosoma vivax and Borrelia burdgorferi in cattle from the Northeastern region of the state of Pará, Brazil. Revista Brasileira de Parasitologia Veterinária, v. 17, n. 2, p. 105-109, abr./jun. 2008.| Biblioteca(s): Embrapa Gado de Corte. |
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| 3. | | SILVA, J. B.; LOPES, C. T. A.; PINHEIRO, C. P.; LIMA, D. H. S.; SILVA, R. S. L.; FONSECA. A. H.; ARAUJO, F. R.; BARBOSA-NETO, J. D. Prevalência sorológica e molecular de Babesia bovis e Babesia bigemina em búfalos (Bubalus bubalis) na Ilha de Marajó, Pará. Pesquisa Veterinária Brasileira, v. 33, n. 7, p.847-850, julho 2013.| Biblioteca(s): Embrapa Gado de Corte. |
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| 4. | | SILVA, J. B. da; LOPES, C. T. de A.; SOUZA, M. G. S. de; GIBSON, A. F. B.; VINHOTE, W. M. de S.; FONSECA, A. H. da; ARAUJO, F. R.; BARBOSA-NETO, J. D. Detecção sorológica e molecular de Anaplasma marginale em búfalos na Ilha de Marajó, Pará1. Pesquisa Veterinária Brasileira, v. 34, n. 1, p. 11-14, jan., 2014| Biblioteca(s): Embrapa Gado de Corte. |
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| 5. | | ARAÚJO, C. P.; OSÓRIO, A. L. A. R.; JORGE, K. S. G.; RAMOS, C. A. N.; SOUZA FILHO, A. F.; VIDAL, C. E. S.; VARGAS, A. P. C.; ROXO, E; ROCHA, A. S.; SUFFYS, P. N.; FONSECA JÚNIOR, A. A.; SILVA, M. R.; BARBOSA NETO, J. D.; CERQUEIRA, V. D.; ARAUJO, F. R. Direct detection of Mycobacterium tuberculosis complex in bovine and bubaline tissues through nested-PCR. Brazilian Journal of Microbiology 45, 2, 2014, P. 633-640., 2014.| Biblioteca(s): Embrapa Gado de Corte; Embrapa Gado de Leite. |
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| Registros recuperados : 5 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
18/06/2018 |
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Data da última atualização: |
18/06/2018 |
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Autoria: |
ANDRADE, C. P.; BARBOSA NETO, J. D.; DRIEMEIER, D. |
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Afiliação: |
Caroline P. Andrade; José D. Barbosa Neto; David Driemeier. |
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Título: |
Identification of single nucleotide polymorphisms in the prion protein gene in Santa Ines and Dorset sheep. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 4, p. 624-628, abril 2018 |
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Idioma: |
Inglês |
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Notas: |
Título em português: Identificação de polimorfismos de nucleotídeos únicos em ovinos Santa Inês e Dorset através do gene da proteína priônica. |
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Conteúdo: |
Scrapie is a transmissible spongiform encephalopathy (TSE) that affects sheep and goats and results from accumulation of the abnormal isoform of a prion protein in the central nervous system. Resistance or susceptibility to the disease is dependent on several factors, including the strain of infecting agent, the degree of exposure, and the presence of single nucleotide polymorphisms (SNPs) in the prion protein gene. The most important polymorphisms are present in codons 136, 154, and 171. SNPs have also been identified in other codons, such as 118, 127, 141, 142, and 143. The objective of this study was to investigate the genotypic profile of Santa Ines (n=94) and Dorset (n=69) sheep and identify polymorphisms in the prion protein gene using real-time PCR techniques and sequencing. We analyzed SNPs in 10 different codons (127, 136, 138, 140, 141, 142, 143, 154, 171, and 172) in Santa Ines sheep. Classification of the flock into risk groups associated with scrapie revealed that approximately 68% of the Santa Ines herd was considered at moderate risk (group 3), and the most frequent haplotype was ARQ/ARQ (47.8%). For Dorset sheep, 42% of the herd was considered at moderate risk (group 3), 40% at low risk (group 2), and 12% at very low risk (group 1). These findings improve our understanding of the genotype breed and further highlight the importance of genotyping and identification of polymorphisms in Brazilian herds to assess their effects on potential infections upon exposure to the sheep prion. MenosScrapie is a transmissible spongiform encephalopathy (TSE) that affects sheep and goats and results from accumulation of the abnormal isoform of a prion protein in the central nervous system. Resistance or susceptibility to the disease is dependent on several factors, including the strain of infecting agent, the degree of exposure, and the presence of single nucleotide polymorphisms (SNPs) in the prion protein gene. The most important polymorphisms are present in codons 136, 154, and 171. SNPs have also been identified in other codons, such as 118, 127, 141, 142, and 143. The objective of this study was to investigate the genotypic profile of Santa Ines (n=94) and Dorset (n=69) sheep and identify polymorphisms in the prion protein gene using real-time PCR techniques and sequencing. We analyzed SNPs in 10 different codons (127, 136, 138, 140, 141, 142, 143, 154, 171, and 172) in Santa Ines sheep. Classification of the flock into risk groups associated with scrapie revealed that approximately 68% of the Santa Ines herd was considered at moderate risk (group 3), and the most frequent haplotype was ARQ/ARQ (47.8%). For Dorset sheep, 42% of the herd was considered at moderate risk (group 3), 40% at low risk (group 2), and 12% at very low risk (group 1). These findings improve our understanding of the genotype breed and further highlight the importance of genotyping and identification of polymorphisms in Brazilian herds to assess their effects on potential infections upon exposure... Mostrar Tudo |
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Palavras-Chave: |
Genotipagem; Nulceotídeos únicos; Proteína priônica. |
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Thesagro: |
Ovino; Polimorfismo. |
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Thesaurus NAL: |
Genotyping; Sheep; Single nucleotide polymorphism. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/178696/1/Identification-of-single-nucleotide-polymorphisms.pdf
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Marc: |
LEADER 02443naa a2200253 a 4500
001 2092531
005 2018-06-18
008 2018 bl uuuu u00u1 u #d
100 1 $aANDRADE, C. P.
245 $aIdentification of single nucleotide polymorphisms in the prion protein gene in Santa Ines and Dorset sheep.$h[electronic resource]
260 $c2018
500 $aTítulo em português: Identificação de polimorfismos de nucleotídeos únicos em ovinos Santa Inês e Dorset através do gene da proteína priônica.
520 $aScrapie is a transmissible spongiform encephalopathy (TSE) that affects sheep and goats and results from accumulation of the abnormal isoform of a prion protein in the central nervous system. Resistance or susceptibility to the disease is dependent on several factors, including the strain of infecting agent, the degree of exposure, and the presence of single nucleotide polymorphisms (SNPs) in the prion protein gene. The most important polymorphisms are present in codons 136, 154, and 171. SNPs have also been identified in other codons, such as 118, 127, 141, 142, and 143. The objective of this study was to investigate the genotypic profile of Santa Ines (n=94) and Dorset (n=69) sheep and identify polymorphisms in the prion protein gene using real-time PCR techniques and sequencing. We analyzed SNPs in 10 different codons (127, 136, 138, 140, 141, 142, 143, 154, 171, and 172) in Santa Ines sheep. Classification of the flock into risk groups associated with scrapie revealed that approximately 68% of the Santa Ines herd was considered at moderate risk (group 3), and the most frequent haplotype was ARQ/ARQ (47.8%). For Dorset sheep, 42% of the herd was considered at moderate risk (group 3), 40% at low risk (group 2), and 12% at very low risk (group 1). These findings improve our understanding of the genotype breed and further highlight the importance of genotyping and identification of polymorphisms in Brazilian herds to assess their effects on potential infections upon exposure to the sheep prion.
650 $aGenotyping
650 $aSheep
650 $aSingle nucleotide polymorphism
650 $aOvino
650 $aPolimorfismo
653 $aGenotipagem
653 $aNulceotídeos únicos
653 $aProteína priônica
700 1 $aBARBOSA NETO, J. D.
700 1 $aDRIEMEIER, D.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 38, n. 4, p. 624-628, abril 2018
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Embrapa Unidades Centrais (AI-SEDE) |
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