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 | Acesso ao texto completo restrito à biblioteca da Embrapa Arroz e Feijão. Para informações adicionais entre em contato com cnpaf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
01/09/2015 |
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Data da última atualização: |
29/03/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
OLIVEIRA, M. B.; LOBO JUNIOR, M.; GROSSI-DE-SÁ, M. F.; PETROFEZA, S. |
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Afiliação: |
MARÍLIA BARROS OLIVEIRA, UFG; MURILLO LOBO JUNIOR, CNPAF; MARIA FATIMA GROSSI DE SA, CENARGEN; SILVANA PETROFEZA, UFG. |
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Título: |
Exogenous application of methyl jasmonate induces a defense response and resistance against Sclerotinia sclerotiorum in dry bean plants. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Journal of Plant Physiology, v. 182, p. 13-22, June 2015. |
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DOI: |
10.1016/j.jplph.2015.04.006 |
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Idioma: |
Inglês |
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Conteúdo: |
Sclerotinia sclerotiorum (Lib.) de Bary is a necrotrophic fungal pathogen that causes a disease known as white mold, which is a major problem for dry bean (Phaseolus vulgaris L.) and other crops in many growing areas in Brazil. To investigate the role of methyl jasmonate (MeJA) in defending dry bean plants against S. sclerotiorum, we used suppression subtractive hybridization (SSH) of cDNA and identified genes that are differentially expressed during plant?pathogen interactions after treatment. Exogenous MeJA application enhanced resistance to the pathogen, and SSH analyses led to the identification of 94 unigenes, presumably involved in a variety of functions, which were classified into several functional categories, including metabolism, signal transduction, protein biogenesis and degradation, and cell defense and rescue. Using RT?qPCR, some unigenes were found to be differentially expressed in a time-dependent manner in dry bean plants during the interaction with S. sclerotiorum after MeJA treatment, including the pathogenes is related protein PR3 (chitinase), PvCallose (callose synthase), PvNBS-LRR (NBS-LRR resistance-like protein), PvF-box (F-box family protein-like), and a polygalacturonase inhibitor protein (PGIP). Based on these expression data, the putative roles of differentially expressed genes were discussed in relation to the disease and MeJA resistance induction. Changes in the activity of the pathogenesis-related proteins - 1,3-glucanase, chitinase, phenylalanine ammonia-lyase, and peroxidase in plants after MeJA treatment and following inoculation of the pathogen were also investigated as molecular markers of induced resistance. Foliar application of MeJA induced partial resistance against S. sclerotiorum in plants as well as a consistent increase in pathogenesis-related protein activities. Our findings provide new insights into the physiological and molecular mechanisms of resistance induced by MeJA in the P. vulgaris - S. sclerotiorum pathosystem. MenosSclerotinia sclerotiorum (Lib.) de Bary is a necrotrophic fungal pathogen that causes a disease known as white mold, which is a major problem for dry bean (Phaseolus vulgaris L.) and other crops in many growing areas in Brazil. To investigate the role of methyl jasmonate (MeJA) in defending dry bean plants against S. sclerotiorum, we used suppression subtractive hybridization (SSH) of cDNA and identified genes that are differentially expressed during plant?pathogen interactions after treatment. Exogenous MeJA application enhanced resistance to the pathogen, and SSH analyses led to the identification of 94 unigenes, presumably involved in a variety of functions, which were classified into several functional categories, including metabolism, signal transduction, protein biogenesis and degradation, and cell defense and rescue. Using RT?qPCR, some unigenes were found to be differentially expressed in a time-dependent manner in dry bean plants during the interaction with S. sclerotiorum after MeJA treatment, including the pathogenes is related protein PR3 (chitinase), PvCallose (callose synthase), PvNBS-LRR (NBS-LRR resistance-like protein), PvF-box (F-box family protein-like), and a polygalacturonase inhibitor protein (PGIP). Based on these expression data, the putative roles of differentially expressed genes were discussed in relation to the disease and MeJA resistance induction. Changes in the activity of the pathogenesis-related proteins - 1,3-glucanase, chitinase, phenylalan... Mostrar Tudo |
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Palavras-Chave: |
Genes relacionadas com patogénese. |
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Thesagro: |
Doença de planta; Feijão; Fungo; Mofo Branco; Phaseolus vulgaris; Sclerotinia sclerotiorum. |
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Thesaurus Nal: |
Dry beans; Methyl jasmonate. |
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Categoria do assunto: |
--
H Saúde e Patologia |
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Marc: |
LEADER 02883naa a2200277 a 4500
001 2025021
005 2016-03-29
008 2015 bl uuuu u00u1 u #d
024 7 $a10.1016/j.jplph.2015.04.006$2DOI
100 1 $aOLIVEIRA, M. B.
245 $aExogenous application of methyl jasmonate induces a defense response and resistance against Sclerotinia sclerotiorum in dry bean plants.$h[electronic resource]
260 $c2015
520 $aSclerotinia sclerotiorum (Lib.) de Bary is a necrotrophic fungal pathogen that causes a disease known as white mold, which is a major problem for dry bean (Phaseolus vulgaris L.) and other crops in many growing areas in Brazil. To investigate the role of methyl jasmonate (MeJA) in defending dry bean plants against S. sclerotiorum, we used suppression subtractive hybridization (SSH) of cDNA and identified genes that are differentially expressed during plant?pathogen interactions after treatment. Exogenous MeJA application enhanced resistance to the pathogen, and SSH analyses led to the identification of 94 unigenes, presumably involved in a variety of functions, which were classified into several functional categories, including metabolism, signal transduction, protein biogenesis and degradation, and cell defense and rescue. Using RT?qPCR, some unigenes were found to be differentially expressed in a time-dependent manner in dry bean plants during the interaction with S. sclerotiorum after MeJA treatment, including the pathogenes is related protein PR3 (chitinase), PvCallose (callose synthase), PvNBS-LRR (NBS-LRR resistance-like protein), PvF-box (F-box family protein-like), and a polygalacturonase inhibitor protein (PGIP). Based on these expression data, the putative roles of differentially expressed genes were discussed in relation to the disease and MeJA resistance induction. Changes in the activity of the pathogenesis-related proteins - 1,3-glucanase, chitinase, phenylalanine ammonia-lyase, and peroxidase in plants after MeJA treatment and following inoculation of the pathogen were also investigated as molecular markers of induced resistance. Foliar application of MeJA induced partial resistance against S. sclerotiorum in plants as well as a consistent increase in pathogenesis-related protein activities. Our findings provide new insights into the physiological and molecular mechanisms of resistance induced by MeJA in the P. vulgaris - S. sclerotiorum pathosystem.
650 $aDry beans
650 $aMethyl jasmonate
650 $aDoença de planta
650 $aFeijão
650 $aFungo
650 $aMofo Branco
650 $aPhaseolus vulgaris
650 $aSclerotinia sclerotiorum
653 $aGenes relacionadas com patogénese
700 1 $aLOBO JUNIOR, M.
700 1 $aGROSSI-DE-SÁ, M. F.
700 1 $aPETROFEZA, S.
773 $tJournal of Plant Physiology$gv. 182, p. 13-22, June 2015.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Meio Ambiente. Para informações adicionais entre em contato com cnpma.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
19/03/2014 |
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Data da última atualização: |
31/10/2017 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
TERAO, D.; BATISTA, D. da C.; BARBOSA, M. A. G. |
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Afiliação: |
DANIEL TERAO, CPATSA; DIOGENES DA CRUZ BATISTA, CPATSA; MARIA ANGELICA GUIMARAES BARBOSA, CPATSA. |
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Título: |
Integração de estratégias alternativas no controle de doenças pós-colheita de manga na região Semiárida do Brasil. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
In: SIMPÓSIO BRASILEIRO DE PÓS-COLHEITA DE FRUTAS, HORTALIÇAS E FLORES, 4.; ENCONTRO NACIONAL SOBRE PROCESSAMENTO MÍNIMO DE FRUTAS E HORTALIÇAS, 7., 2013, Ribeirão Preto. Internacionalizar para não perecer: anais. Ribeirão Preto: USP-Faculdade de Cências Farmacêuticas de Ribeirão Preto, 2013. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Português |
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Conteúdo: |
Este trabalho teve por objetivo identificar os principais fungos causadores de podridões em manga, estudar aspectos epidemiológicos que permitam recomendar medidas culturais e aplicações de fungicidas no campo e avaliar métodos de controle físicos na pós-colheita, a fim de apresentar um manejo integrado que possibilite a produção de manga de qualidade, sem resíduos de agrotóxicos. |
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Palavras-Chave: |
Disease; Manejo integrado; Mango; Postharvest. |
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Thesagro: |
Controle biológico; Doença; Manga; Mangifera Indica; Pós-colheita. |
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Categoria do assunto: |
O Insetos e Entomologia |
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Marc: |
LEADER 01408naa a2200265 a 4500
001 1982819
005 2017-10-31
008 2013 bl --- 0-- u #d
100 1 $aTERAO, D.
245 $aIntegração de estratégias alternativas no controle de doenças pós-colheita de manga na região Semiárida do Brasil.$h[electronic resource]
260 $c2013
300 $c1 CD-ROM.
520 $aEste trabalho teve por objetivo identificar os principais fungos causadores de podridões em manga, estudar aspectos epidemiológicos que permitam recomendar medidas culturais e aplicações de fungicidas no campo e avaliar métodos de controle físicos na pós-colheita, a fim de apresentar um manejo integrado que possibilite a produção de manga de qualidade, sem resíduos de agrotóxicos.
650 $aControle biológico
650 $aDoença
650 $aManga
650 $aMangifera Indica
650 $aPós-colheita
653 $aDisease
653 $aManejo integrado
653 $aMango
653 $aPostharvest
700 1 $aBATISTA, D. da C.
700 1 $aBARBOSA, M. A. G.
773 $tIn: SIMPÓSIO BRASILEIRO DE PÓS-COLHEITA DE FRUTAS, HORTALIÇAS E FLORES, 4.; ENCONTRO NACIONAL SOBRE PROCESSAMENTO MÍNIMO DE FRUTAS E HORTALIÇAS, 7., 2013, Ribeirão Preto. Internacionalizar para não perecer: anais. Ribeirão Preto: USP-Faculdade de Cências Farmacêuticas de Ribeirão Preto, 2013.
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Embrapa Meio Ambiente (CNPMA) |
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