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| Registros recuperados : 2 | |
| 1. |  | MCCLURE, M. C.; SONSTEGARD, T. S.; WIGGANS, G.; EENENNAAM, A. L. V.; WEBER, K. L.; PENEDO, C. T.; BERRY, D. P.; FLYNN, J.; GARCIA, J. F.; CARMO, A. S.; REGITANO, L. C. A.; SOUZA, M. A.; SILVA, M. V. G. B.; COFFEY, M.; MOORE, K.; BOSCHER, M. Y.; GENESTOUT, L.; MAZZA, R.; TAYLOR, J. F.; SCHNABEL, R. D.; MACHADO, M. A.; SIMPSON, B.; MCEWAN, J. C.; CROMIE, A.; COUTINHO, L. L.; KUEHN, L. A.; KEELE, J. W.; PIPER, E. K.; COOK, J.; MARQUES, E.; TASSELL, C. P. V. Imputation of microsatellite alleles from dense SNP genotypes for parentage verification across multiple bos taurus and bos indicus breeds. In: ANNUAL INTERNATIONAL PLANT AND ANIMAL GENOME CONFERENCE, 21., 2013, San Diego. Abstracts... San Diego: [s.n.], 2013.| Biblioteca(s): Embrapa Gado de Leite. |
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| 2. | | McCLURE, M. C.; SONSTEGARD, T. S.; WIGGANS, G. R.; EENENNAAM, A. L. V.; WEBER, K. L.; PENEDO, C. T.; BERRY, D. P.; FLYNN, J.; GARCIA, J. F.; CARMO, A. S.; REGITANO, L. C. de A.; ALBUQUERQUE, M.; SILVA, M. V. G. B.; MACHADO, M. A.; COFFEY, M.; MOORE, K.; BOSCHER, M. Y.; GENESTOUT, L.; MAZZA, R.; TAYLOR, J. F.; SCHNABEL, R. D.; SIMPSON, B.; MARQUES, E.; McEWAN, J. C.; CROMIE, A.; COUTINHO, L. L.; KUEHN, L. A.; KEELE, J. W.; PIPER, E. K.; COOK, J.; WILLIAMS, R.; TASSELL, C. P. V. Imputation of microsatellite alleles from dense SNP genotypes for parentage verification across multiple Bos taurus and Bos indicus breeds. Frontiers in Genetics, v. 4, n. 176, 2013. 11 p.| Biblioteca(s): Embrapa Gado de Leite; Embrapa Pecuária Sudeste. |
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| Registros recuperados : 2 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
24/09/2021 |
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Data da última atualização: |
24/09/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
PIRES, D. R.; MORAIS, A. C. N.; CUNHA, N. C.; MACHADO, L. S.; BARBOSA, L. F. C.; MENDONÇA, J. F. M.; BALARO, M. F. A.; SANTOS, J. P. C.; SOUZA, G. N. de; BARRETO, M. L.; NASCIMENTO, E. R. |
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Afiliação: |
Universidade Federal Fluminense; Universidade Federal Fluminense; Universidade Federal Fluminense; Universidade Federal Fluminense; Universidade Federal Fluminense; Universidade Federal Fluminense; Universidade Federal Fluminense; Fundação Oswaldo Cruz; GUILHERME NUNES DE SOUZA, CNPGL; Universidade Federal Fluminense; Universidade Federal Fluminense. |
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Título: |
Proposal of an iELISA for Mycoplasma bovis diagnosis in dairy cattle and associated risk factors. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, v. 73, n. 2, p. 293-301, 2021. |
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Idioma: |
Inglês |
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Conteúdo: |
Mycoplasma bovis is a highly contagious agent associated with several pathologies in cattle. The detection of reactive antibodies to M. bovis by Indirect Enzyme-Linked Immunosorbent Assay (iELISA) identifies if there was an exposure to the microorganism. The current study aimed to optimize an iELISA from M. bovis total cell antigen, applying it to bovine serum samples, and to evaluate risk factors. Serum samples were obtained from 400 cows from 17 herds from Southeast Brazil. In the optimization of iELISA, the following was established: 2 ?g/mL of antigen, sera dilution 1:300, and conjugate dilution 1:15000. The frequency was 62.3% (249/400) of reactive animals and 100% (17/17) of reactive herds. Risk factors were: herds with more than 100 animals (OR= 3.1; CI= 95%); Holstein breed (OR= 72.5; CI= 95%); cows (OR= 29.7; CI= 95%); intensive breeding system (OR= 3.3; CI= 95%); associated small ruminant production (OR= 4.4; CI= 95%); milk production above 500L (OR= 2.9; CI= 95%); no quarantine (OR= 1.5; CI= 95%); mechanical milking (OR= 5.5; CI= 95%) and cases of mastitis (OR=5.5; CI= 95%). The proposed iELISA was able to detect antibodies reactive to M. bovis in bovine serum. Knowledge of these risk factors can assist in the implementation of prophylactic measures. |
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Palavras-Chave: |
Holstein breed; Mastite; Raça Holandesa. |
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Thesagro: |
Bovino; Micoplasmose; Vaca. |
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Thesaurus NAL: |
Cows; Mastitis; Mycoplasmosis; Serology. |
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Categoria do assunto: |
K Ciência Florestal e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/226352/1/Proposal-iELISA.pdf
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Marc: |
LEADER 02298naa a2200361 a 4500
001 2134749
005 2021-09-24
008 2021 bl uuuu u00u1 u #d
100 1 $aPIRES, D. R.
245 $aProposal of an iELISA for Mycoplasma bovis diagnosis in dairy cattle and associated risk factors.$h[electronic resource]
260 $c2021
520 $aMycoplasma bovis is a highly contagious agent associated with several pathologies in cattle. The detection of reactive antibodies to M. bovis by Indirect Enzyme-Linked Immunosorbent Assay (iELISA) identifies if there was an exposure to the microorganism. The current study aimed to optimize an iELISA from M. bovis total cell antigen, applying it to bovine serum samples, and to evaluate risk factors. Serum samples were obtained from 400 cows from 17 herds from Southeast Brazil. In the optimization of iELISA, the following was established: 2 ?g/mL of antigen, sera dilution 1:300, and conjugate dilution 1:15000. The frequency was 62.3% (249/400) of reactive animals and 100% (17/17) of reactive herds. Risk factors were: herds with more than 100 animals (OR= 3.1; CI= 95%); Holstein breed (OR= 72.5; CI= 95%); cows (OR= 29.7; CI= 95%); intensive breeding system (OR= 3.3; CI= 95%); associated small ruminant production (OR= 4.4; CI= 95%); milk production above 500L (OR= 2.9; CI= 95%); no quarantine (OR= 1.5; CI= 95%); mechanical milking (OR= 5.5; CI= 95%) and cases of mastitis (OR=5.5; CI= 95%). The proposed iELISA was able to detect antibodies reactive to M. bovis in bovine serum. Knowledge of these risk factors can assist in the implementation of prophylactic measures.
650 $aCows
650 $aMastitis
650 $aMycoplasmosis
650 $aSerology
650 $aBovino
650 $aMicoplasmose
650 $aVaca
653 $aHolstein breed
653 $aMastite
653 $aRaça Holandesa
700 1 $aMORAIS, A. C. N.
700 1 $aCUNHA, N. C.
700 1 $aMACHADO, L. S.
700 1 $aBARBOSA, L. F. C.
700 1 $aMENDONÇA, J. F. M.
700 1 $aBALARO, M. F. A.
700 1 $aSANTOS, J. P. C.
700 1 $aSOUZA, G. N. de
700 1 $aBARRETO, M. L.
700 1 $aNASCIMENTO, E. R.
773 $tArquivo Brasileiro de Medicina Veterinária e Zootecnia$gv. 73, n. 2, p. 293-301, 2021.
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Embrapa Gado de Leite (CNPGL) |
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