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| Registros recuperados : 55 | |
| 21. |  | CASTRO, M. E. B.; MELO, F. L.; TAGLIARI, M.; INGLIS, P. W.; CRAVEIRO, S. R.; RIBEIRO, Z. M. A.; RIBEIRO, B. M.; BÁO, S. N. The genome sequence of Condylorrhiza vestigialis NPV, a novel baculovirus for the control of the Alamo moth on Populus spp. in Brazil. Journal of Invertebrate Pathology, September, v. 148, p.152-161, 2017.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 22. | | ARDISSON-ARAÚJO, D. M. P.; LIMA, R. N.; MELO, F. L.; CLEM, R. J.; HUANG, N.; BÁO, S. N.; SOSA-GÓMEZ, D. R.; RIBEIRO, B. M. Genome sequence of Perigonia lusca single nucleopolyhedrovirus: insights into the evolution of a nucleotide metabolism enzyme in the family Baculoviridae. Scientific Reports, v. 6, n. 24612, jun. 2016.| Biblioteca(s): Embrapa Soja. |
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| 23. | | SILVA, C. G. da; MARTINS, C. F.; CARDOSO, T. C.; CUNHA, E. R. da; CUMPA, H. C. B.; MCMANUS, C. M.; PIVATO, I.; BAO, S. N. Isolation and characterization of mesenchymal stem cells derived from bovine Wharton's jelly and their potential for use in cloning by nuclear transfer. Isolamento e caracterização de células tronco mesenquimais derivadas de geléia de Wharton bovina e seu potencial para uso na clonagem por transferência nuclear. Ciência Rural, Santa Maria, v. 46, n. 10, p. 1830-1837, out. 2016.| Biblioteca(s): Embrapa Cerrados. |
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| 24. | | FARIAS, L. R. de; BÁO, S. N.; ZHOU, J. J.; PAPPAS JUNIOR, G. J.; MORAES, M. C. B.; LAUMANN, R. A.; BORGES, M. Estudo da expressão de genes relacionados à olfação no percevejo-marrom, Euschistus Heros (Hemiptera: Pentatimidae), praga da soja. In: CONGRESSO BRASILEIRO DE ENTOMOLOGIA, 23., 2010, Natal, RN. Anais... Natal: Sociedade Brasileira de Entomologia, 2010. CD-ROM.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 25. | | LUCCI, C. M.; BÁO, S. N.; SILVA, J. R. V.; AMORIM, C. A.; RODRIGUES, A. P. R.; GONÇALVES, P. B. D.; FIGUEIREDO, J. R. Desenvolvimento de um método mecânico específico para o isolamento de folículos ovarianos pre-antrais intactos a partir de ovários em caprinos. Ciência Animal, v. 7, n. 2, p. 110, jul./dez. 1997. Edição do II Simpósio Nacional de Biotecnologia da Reprodução de Animais Domésticos, 1997, Fortaleza.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 26. | | MONDADORI, R. G.; SANTIN, T. R.; FIDELIS, A. A. G.; NAME, K. P. O.; DA SILVA, J. S.; RUMPF, T.; BÁO, S. N. Ultraestrutura da maturação in vitro de oócitos bubalinos (Bubalus bubalis). Acta Scientiae Veterinariae, v. 36, supl. 2, p. S498, 2008. Edição dos resumos da 22. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Guarujá, SP, 2008.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 28. | | RICARTE, A. R. F.; TEIXEIRA, M. F. da S.; ANDRIOLI, A.; PINHEIRO, R. R.; BÁO, S. N.; SILVA, J. B. A. da. Análise molecular e ultraestrutural de espermatozóides caprinos oriundos de animais infectados naturalmente e experimentalmente com o CAEV. In: CONGRESSO NORDESTINO DE PRODUÇÃO ANIMAL, 6.; SIMPÓSIO NORDESTINO DE ALIMENTAÇÃO DE RUMINANTES, 7.; FÓRUM DE COORDENADORES DE PÓS GRADUAÇÃO EM PRODUÇÃO ANIMAL DO NORDESTE, 1.; FÓRUM DE AGROECOLOGIA RO RIO GRANDE DO NORTE, 1., 2010, Mossoró. Anais... Mossoró: Sociedade Nordestina de Producao Animal; UFERSA, 2010. 4 f. 1 CD-ROM.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 29. | | MATOS, M. H. T.; SILVA, J. R. V.; LUCCI, C. M.; BÁO, S. N.; SANTOS, R. R.; COSTA, S. H. F.; CELESTINO, J. J. H.; FIGUEIREDO, J. R. Análise morfológica e ultraestrutural de folículos primordiais ovinos conservados em solução salina 0,9% e TCM 199. Acta Scientiae Veterinariae, Porto Alegre, v. 31, p. 468, 2003. Suplemento. Edição de anais da 17a. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Beberibe, CE, ago. 2003.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 30. | | PARENTE, A. F.; SILVA-PEREIRA, I.; BALDANI, J. I.; TIBÚRCIO, V. H. da S.; BÁO, S. N.; DE SOUZA, M. T. Construction of Bacillus thuringiensis wild-type S76 and Cry- derivatives expressing a green fluorescent protein: two potential marker organisms to study bacteria-plant interactions. Canadian Journal of Microbiology, Ottawa, v. 54, p. 786-790, 2008. Parceria: UnB.| Biblioteca(s): Embrapa Agrobiologia. |
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| 31. | | BAPTISTELLA, J. C.; SILVA, C. G. da; BÁO, S. N.; PANEGOSSI, L. C.; CARDOSO, T. C.; CARVALHO, R. G. de; MARTINS, C. F. Immunomodulatory-associated gene transcripts to multipotency of bovine amniotic fluid mesenchymal stem cells. Animal Reproduction, v. 21, n. 1, e20230155, 2024.| Biblioteca(s): Embrapa Cerrados. |
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| 33. | | JOVITO, B. L.; PATERNO, L. G.; SALES, M. J. A.; GROSS, M. A.; SILVA, L. P. da; SOUZA, P. de; BÁO, S. N. Graphene oxide/zinc oxide nanocomposite displaying selective toxicity to glioblastoma cell lines. ACS Applied Bio Materials, v. 4, p. 829-843, 2021. Na publicação: Luciano P. Silva.| Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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| 34. | | PARENTE, A. F.; BALDANI, J. I.; PEREIRA, I. S.; TIBÚRCIO, V. H. da S.; BÁO, S. N.; DE SOUZA, M. T. GFP expression in wild-type B. thuringiensis strain active to Lepidoptera. In: MEETING OF THE SOCIETY FOR INVERTEBRATE PATHOLOGY, 40th., INTERNATIONAL FORUM ON ENTOMOPATHOGENIC NEMATODES AND SYMBIOTIC BACTERIA, 1st., 2007, Quebec City. Anais... Quebec: Society for Invertebrate Pathology, 2007. p. 59. Parceria: UnB.| Biblioteca(s): Embrapa Agrobiologia. |
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| 35. | | SILVA, C. G. da; MARTINS, C. F.; CUMPA, H. C. B.; MENDONÇA, A. dos S.; FRANCO, M. M.; BÁO, S. N. Metilação e hidroximetilação global de células bovinas derivadas do fluido aminiótico, tecido adiposo e fibroblastos da pele e seu uso na clonagem na presença ou ausência de tricostatina A. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA CERRADOS. Jovens talentos 2016: resumos. Planaltina, DF: Embrapa Cerrados, 2016. 99 p. p. 29 (Embrapa Cerrados. Documentos, 334). Comissão organizadora: SOUZA, K. W. de; FALEIRO, A. S. G.; SALGUES, A. L. M.; ALONSO, A. M.; CARVALHO, A. M. de; LOBATO, B. R.; CRUZ, C. V.; SOUSA, E. dos S.; PELEGRINELLI, F.; SOARES, J. P. G.; LIMA, J. E. F. W.; ARBUES, J. F. de O.;...| Biblioteca(s): Embrapa Cerrados. |
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| 36. | | SILVA, C. G. da; CUNHA, E. R. da; MARTINS, C. F.; PIVATO, I.; BESSLER, H. C.; LIMA MARTINS, G. H.; BÁO, S. N. Prenhezes e nascimentos de bovinos clonados a partir de células do fluído amniótico e do tecido adiposo in vitro. In: REUNIÃO ANUAL [DA] SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 27., 2013, Praia do Forte, BA. Resumos... Praia do Forte: SBTE, 2013. p. 401.| Biblioteca(s): Embrapa Cerrados. |
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| 37. | | SILVA, C. G.; MARTINS, C. F.; CARDOSO, T. C.; CUNHA, E. R. da; CUMPA, H. C. B.; MARTINS, G. H. L.; PIVATO, I.; BÁO S. N. Production of bovine embryos and calves cloned by nuclear transfer using mesenchymal stem cells from amniotic fluid and adipose tissue. Cellular Reprogramming, v. 18, n. 2, p. 127-136, 2016. p. 127-136| Biblioteca(s): Embrapa Cerrados. |
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| 38. | | RODRIGUES, A. P. R.; AMORIM, C. A.; LUCCI, C. M.; BAO, S. N.; BRASIL, A. F.; COSTA, S. H. F.; FIGUEIREDO, J. R. Toxicity test and cryopreservation of preantral follicles enclosed in caprine ovarian tissue. Theriogenology, v.57, n.1, p.478, 2002. CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY. Proceedings... Editado por: Mtthew B. Wheeler; Lawrence Smith.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| 39. | | SILVA, C. G. da; BÁO, S. N.; CUMPA, H. C. B.; CARDOSO, T. C.; FONSECA, L. O. da; FONSECA NETO, A. M. da; GODOY, S. D.; MARTINS, G. H.; CUNHA, E. R. da; MARTINS, C. F. Brasília da Cerrados: primeiro bovino clonado utilizando células-tronco mesenquimais do tecido adiposo. Planaltina, DF: Embrapa Cerrados, 2016. 34 p. (Embrapa Cerrados. Documentos, 330)| Biblioteca(s): Embrapa Cerrados. |
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| 40. | | RODRIGUES, A. P. R.; AMORIM, C. A.; COSTA, S. H. F.; MATOS, M. H. T.; SANTOS, R. R.; LUCCI, C. M.; BÁO, S. N.; OHASHI, O. M.; FIGUEIREDO, J. R. Criopreservação de tecido ovariano caprino usando diferentes crioprotetores. Acta Scientiae Veterinariae, Porto Alegre, v. 31, p. 546, 2003. Suplemento. Edição de anais da 17a. Reunião Anual da Sociedade Brasileira de Tecnologia de Embriões, Beberibe, CE, ago. 2003.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| Registros recuperados : 55 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
08/12/2011 |
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Data da última atualização: |
05/02/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SILVA, I. O. e; VASCONCELOS, R. B.; CAETANO, J. V. O.; GULART, L. V. M.; CAMARGO, L. S. de A.; BÁO, S. N.; SILVA, A. A. M. R. e. |
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Afiliação: |
I. OLIVEIRA E SILVA, UNB; R. B. VASCONCELOS, UNB; J. V. O. CAETANO, UNB; L. V. M. GULART, UNB; LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL; S. N. BÁO, UNB; A. A. M. ROSA E SILVA, UNB. |
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Título: |
Induction of reversible meiosis arrest of bovine oocytes using a two-step procedure under defined and nondefined conditions. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Theriogenology, v. 75, n. 6, p. 1115-1124, 2011. |
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DOI: |
https://doi.org/10.1016/j.theriogenology.2010.11.021 |
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Idioma: |
Inglês |
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Conteúdo: |
The objective was to study the effect of a defined culture system, on nuclear and cytoplasmic maturation of bovine oocytes, using the two-step procedure of IVM to detect possible inhibition and subsequent resumption of meiosis arrest. In the first step, called the prematuration period (PMP), COCs were cultured in T1-non-defined medium (NDM), or T2-defined medium (DM), both for 24 h. In step 2, called the resumption period (RP), COCs were cultured in: NDM (T1); DM + NDM (T3); or DM+DM (T4) for 24 h in each medium. The NDM was composed of TCM-199 supplemented with FCS and FSH. The DM was composed of alpha-MEM supplemented with PVA, insulin, IGF-1, androstenedione, nonessential amino acids, transferrin, and sodium selenium. Oocytes from T2 had a lower (P < 0.05) rate of nuclear maturation (19.8%) than T1 oocytes (83.2%). Also, T2 COCs appeared to be in the process of cytoplasmic maturation, according to the distribution of organelles assessed by transmission electron microscopy (MET). These COCs had characteristics previously described as mature: erect microvilli on the plasmembrane, presence of cortical/evenly distributed mitochondria throughout the ooplasm, and presence of 50% aligned/cluster cortical granules. Immature characteristics such as small PvS, compact cumulus cells, and presence of 50% cortical granule clusters were also observed. The T1 COCs had only characteristics of maturation (P < 0.05). In step 2 (RP), meiosis arrest induced by DM was resumed after an additional 24 h of culture in NDM (T3) with 79.2% mature COCs, whereas in T4, meiosis arrest was maintained, resulting in almost 70% immature COCs (P < 0.05). At the end of RP, T3 COCs had the mature characteristics of mitochondria spread throughout the cytoplasm (P < 0.05), cumulus expansion, and alignment of cortical granules, whereas the T4 group had both immature and mature characteristics. We inferred that DM can be used in lieu of meiosis inhibitors and furthermore, it can provide extra time to study nuclear and cytoplasmic maturation synchrony of IVM. MenosThe objective was to study the effect of a defined culture system, on nuclear and cytoplasmic maturation of bovine oocytes, using the two-step procedure of IVM to detect possible inhibition and subsequent resumption of meiosis arrest. In the first step, called the prematuration period (PMP), COCs were cultured in T1-non-defined medium (NDM), or T2-defined medium (DM), both for 24 h. In step 2, called the resumption period (RP), COCs were cultured in: NDM (T1); DM + NDM (T3); or DM+DM (T4) for 24 h in each medium. The NDM was composed of TCM-199 supplemented with FCS and FSH. The DM was composed of alpha-MEM supplemented with PVA, insulin, IGF-1, androstenedione, nonessential amino acids, transferrin, and sodium selenium. Oocytes from T2 had a lower (P < 0.05) rate of nuclear maturation (19.8%) than T1 oocytes (83.2%). Also, T2 COCs appeared to be in the process of cytoplasmic maturation, according to the distribution of organelles assessed by transmission electron microscopy (MET). These COCs had characteristics previously described as mature: erect microvilli on the plasmembrane, presence of cortical/evenly distributed mitochondria throughout the ooplasm, and presence of 50% aligned/cluster cortical granules. Immature characteristics such as small PvS, compact cumulus cells, and presence of 50% cortical granule clusters were also observed. The T1 COCs had only characteristics of maturation (P < 0.05). In step 2 (RP), meiosis arrest induced by DM was resumed after an additio... Mostrar Tudo |
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Palavras-Chave: |
Chemically defined medium; Cytoplasmic maturation; Nuclear maturation. |
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Thesaurus NAL: |
Oocytes. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 02902naa a2200253 a 4500
001 1909108
005 2024-02-05
008 2011 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1016/j.theriogenology.2010.11.021$2DOI
100 1 $aSILVA, I. O. e
245 $aInduction of reversible meiosis arrest of bovine oocytes using a two-step procedure under defined and nondefined conditions.$h[electronic resource]
260 $c2011
520 $aThe objective was to study the effect of a defined culture system, on nuclear and cytoplasmic maturation of bovine oocytes, using the two-step procedure of IVM to detect possible inhibition and subsequent resumption of meiosis arrest. In the first step, called the prematuration period (PMP), COCs were cultured in T1-non-defined medium (NDM), or T2-defined medium (DM), both for 24 h. In step 2, called the resumption period (RP), COCs were cultured in: NDM (T1); DM + NDM (T3); or DM+DM (T4) for 24 h in each medium. The NDM was composed of TCM-199 supplemented with FCS and FSH. The DM was composed of alpha-MEM supplemented with PVA, insulin, IGF-1, androstenedione, nonessential amino acids, transferrin, and sodium selenium. Oocytes from T2 had a lower (P < 0.05) rate of nuclear maturation (19.8%) than T1 oocytes (83.2%). Also, T2 COCs appeared to be in the process of cytoplasmic maturation, according to the distribution of organelles assessed by transmission electron microscopy (MET). These COCs had characteristics previously described as mature: erect microvilli on the plasmembrane, presence of cortical/evenly distributed mitochondria throughout the ooplasm, and presence of 50% aligned/cluster cortical granules. Immature characteristics such as small PvS, compact cumulus cells, and presence of 50% cortical granule clusters were also observed. The T1 COCs had only characteristics of maturation (P < 0.05). In step 2 (RP), meiosis arrest induced by DM was resumed after an additional 24 h of culture in NDM (T3) with 79.2% mature COCs, whereas in T4, meiosis arrest was maintained, resulting in almost 70% immature COCs (P < 0.05). At the end of RP, T3 COCs had the mature characteristics of mitochondria spread throughout the cytoplasm (P < 0.05), cumulus expansion, and alignment of cortical granules, whereas the T4 group had both immature and mature characteristics. We inferred that DM can be used in lieu of meiosis inhibitors and furthermore, it can provide extra time to study nuclear and cytoplasmic maturation synchrony of IVM.
650 $aOocytes
653 $aChemically defined medium
653 $aCytoplasmic maturation
653 $aNuclear maturation
700 1 $aVASCONCELOS, R. B.
700 1 $aCAETANO, J. V. O.
700 1 $aGULART, L. V. M.
700 1 $aCAMARGO, L. S. de A.
700 1 $aBÁO, S. N.
700 1 $aSILVA, A. A. M. R. e
773 $tTheriogenology$gv. 75, n. 6, p. 1115-1124, 2011.
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