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Registro Completo |
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Biblioteca(s): |
Embrapa Soja. |
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Data corrente: |
06/01/1993 |
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Data da última atualização: |
06/01/1993 |
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Autoria: |
STAEHELIN, C.; MULLER, J.; MELLOR, R. B.; WIEMKEN, A.; BOLLER, T. |
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Afiliação: |
Botanisches Institut der Universitat Basel, Hebelstrasse 1, CH-4056 Basel, Switzerland. |
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Título: |
Chitinase and peroxidase in effective (fix) and ineffective (fix) soybean nodules. |
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Ano de publicação: |
1992 |
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Fonte/Imprenta: |
Planta, v.187, p.295-300, 1992. |
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Idioma: |
Inglês |
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Conteúdo: |
Chitinase and peroxidase, two enzymes thought to be involved in the defense of plants against pathogens, were measured in soybean (Glycine max L. Merr.) roots and in nodules colonized by Bradyrhizobium japonicum strains differing in their symbiotic potential. Activities of both enzymes were higher in nodules than in roots. In "effective", nitrogen-fixing nodules, colonized by wild-type bacteria, chitinase and peroxidase activities had low levels in the central infected zone and were enhanced primarily in the nodule cortex. An ascorbate-specific peroxidase, possibly involved in radical scavenging, had similarly high activities in the infected zone and in the cortex. "Ineffective" nodules colonized by bacteria unable to fix nitrogen symbiotically showed a similar distribution of chitinase and peroxidase. In another type of "ineffective" nodule, colonized by a B. japonicum strain eliciting a hypersensitive response, activities of both enzymes were enhanced to a similar degree in the infected zone as well as in the cortex. Tissue prints using a direct assay for peroxidase and a anti-serum against bean chitinase corroborated these results. The antiserum against bean chitinase cross-reacted with a nodule protein of Mr 32 000; it inhibited mos of the chitanase activity in the nodules but barely affected the chitinase in uninfected roots. It is concluded that proteins characteristic of the defense reaction accumulate in the cortex of nodules independently of their ability to fix nitrogen, and in the entire body of ... MenosChitinase and peroxidase, two enzymes thought to be involved in the defense of plants against pathogens, were measured in soybean (Glycine max L. Merr.) roots and in nodules colonized by Bradyrhizobium japonicum strains differing in their symbiotic potential. Activities of both enzymes were higher in nodules than in roots. In "effective", nitrogen-fixing nodules, colonized by wild-type bacteria, chitinase and peroxidase activities had low levels in the central infected zone and were enhanced primarily in the nodule cortex. An ascorbate-specific peroxidase, possibly involved in radical scavenging, had similarly high activities in the infected zone and in the cortex. "Ineffective" nodules colonized by bacteria unable to fix nitrogen symbiotically showed a similar distribution of chitinase and peroxidase. In another type of "ineffective" nodule, colonized by a B. japonicum strain eliciting a hypersensitive response, activities of both enzymes were enhanced to a similar degree in the infected zone as well as in the cortex. Tissue prints using a direct assay for peroxidase and a anti-serum against bean chitinase corroborated these results. The antiserum against bean chitinase cross-reacted with a nodule protein of Mr 32 000; it inhibited mos of the chitanase activity in the nodules but barely affected the chitinase in uninfected roots. It is concluded that proteins characteristic of the defense reaction accumulate in the cortex of nodules independently of their ability to fix nit... Mostrar Tudo |
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Palavras-Chave: |
Chitanase; Glycine; Hypersensitive reaction; Isoenzymes; Nodules. |
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Thesagro: |
Peroxidase. |
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Thesaurus Nal: |
Bradyrhizobium. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02183naa a2200253 a 4500
001 1458238
005 1993-01-06
008 1992 bl --- 0-- u #d
100 1 $aSTAEHELIN, C.
245 $aChitinase and peroxidase in effective (fix) and ineffective (fix) soybean nodules.
260 $c1992
520 $aChitinase and peroxidase, two enzymes thought to be involved in the defense of plants against pathogens, were measured in soybean (Glycine max L. Merr.) roots and in nodules colonized by Bradyrhizobium japonicum strains differing in their symbiotic potential. Activities of both enzymes were higher in nodules than in roots. In "effective", nitrogen-fixing nodules, colonized by wild-type bacteria, chitinase and peroxidase activities had low levels in the central infected zone and were enhanced primarily in the nodule cortex. An ascorbate-specific peroxidase, possibly involved in radical scavenging, had similarly high activities in the infected zone and in the cortex. "Ineffective" nodules colonized by bacteria unable to fix nitrogen symbiotically showed a similar distribution of chitinase and peroxidase. In another type of "ineffective" nodule, colonized by a B. japonicum strain eliciting a hypersensitive response, activities of both enzymes were enhanced to a similar degree in the infected zone as well as in the cortex. Tissue prints using a direct assay for peroxidase and a anti-serum against bean chitinase corroborated these results. The antiserum against bean chitinase cross-reacted with a nodule protein of Mr 32 000; it inhibited mos of the chitanase activity in the nodules but barely affected the chitinase in uninfected roots. It is concluded that proteins characteristic of the defense reaction accumulate in the cortex of nodules independently of their ability to fix nitrogen, and in the entire body of ...
650 $aBradyrhizobium
650 $aPeroxidase
653 $aChitanase
653 $aGlycine
653 $aHypersensitive reaction
653 $aIsoenzymes
653 $aNodules
700 1 $aMULLER, J.
700 1 $aMELLOR, R. B.
700 1 $aWIEMKEN, A.
700 1 $aBOLLER, T.
773 $tPlanta$gv.187, p.295-300, 1992.
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Registro original: |
Embrapa Soja (CNPSO) |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
09/01/2012 |
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Data da última atualização: |
11/04/2018 |
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Autoria: |
GOMES, R. da C.; SIQUEIRA, R. F. de; BALLOU, M. A.; STELLA, T. R.; LEME, P. R. |
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Afiliação: |
Rodrigo da Costa Gomes, Universidade de São Paulo, Faculdade de Zootecnia e Engenharia de Alimentos; Renata Farinelli de Siqueira, Universidade de São Paulo, Faculdade de Zootecnia e Engenharia de Alimentos; Michael Alan Ballou, Texas Tech University, Department of Animal and Food Sciences; Tiago Roberto Stella, Universidade de São Paulo, Faculdade de Zootecnia e Engenharia de Alimentos; Paulo Roberto Leme, Universidade de São Paulo, Faculdade de Zootecnia e Engenharia de Alimentos. |
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Título: |
Hematological profile of beef cattle with divergent residual feed intake, following feed deprivation. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
Pesquisa agropecuária Brasileira, Brasília, DF, v. 46, n. 9, p. 1105-1111, set. 2011 |
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Idioma: |
Inglês |
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Notas: |
Notas Científicas.
Título em português: Perfil hematológico de bovinos de corte com consumo alimentar residual divergente, após privação de alimentos. |
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Conteúdo: |
The objective of this work was to characterize the hematological profiles of steers and bulls, according to residual feed intake (RFI), after feed deprivation. Twenty-month-old Nellore steers and bulls were fed feedlot diets for 70 and 56 days, respectively. RFI was calculated as the difference between actual feed intake and expected feed intake. More and less efficient steers and bulls, according to RFI, were subjected to 24 hours of food deprivation. Blood was sampled prior to and following the withdrawal period. Hematological analyses included total and differential white blood cell count, red blood cell count and morphology, and plasma glucose concentration. Variation in RFI did not influence plasma glucose concentration or blood cell profiles. Glucose concentrations in bulls decreased from 114 to 97 mg dL-1, but remained unchanged in steers, and the neutrophil:lymphocyte ratio increased from 0.39 to 0.57 following deprivation. Hematological profiles do not differ between more and less efficient steers and bulls, according to RFI. |
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Palavras-Chave: |
Eficiência alimentar; Estresse; Feed efficiency; Jejum; Leucócitos. |
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Thesagro: |
Bos Indicus; Stress. |
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Thesaurus NAL: |
fasting; leukocytes. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/51874/1/46n09a19.pdf
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Marc: |
LEADER 02007naa a2200289 a 4500
001 1912063
005 2018-04-11
008 2011 bl uuuu u00u1 u #d
100 1 $aGOMES, R. da C.
245 $aHematological profile of beef cattle with divergent residual feed intake, following feed deprivation.
260 $c2011
500 $aNotas Científicas. Título em português: Perfil hematológico de bovinos de corte com consumo alimentar residual divergente, após privação de alimentos.
520 $aThe objective of this work was to characterize the hematological profiles of steers and bulls, according to residual feed intake (RFI), after feed deprivation. Twenty-month-old Nellore steers and bulls were fed feedlot diets for 70 and 56 days, respectively. RFI was calculated as the difference between actual feed intake and expected feed intake. More and less efficient steers and bulls, according to RFI, were subjected to 24 hours of food deprivation. Blood was sampled prior to and following the withdrawal period. Hematological analyses included total and differential white blood cell count, red blood cell count and morphology, and plasma glucose concentration. Variation in RFI did not influence plasma glucose concentration or blood cell profiles. Glucose concentrations in bulls decreased from 114 to 97 mg dL-1, but remained unchanged in steers, and the neutrophil:lymphocyte ratio increased from 0.39 to 0.57 following deprivation. Hematological profiles do not differ between more and less efficient steers and bulls, according to RFI.
650 $afasting
650 $aleukocytes
650 $aBos Indicus
650 $aStress
653 $aEficiência alimentar
653 $aEstresse
653 $aFeed efficiency
653 $aJejum
653 $aLeucócitos
700 1 $aSIQUEIRA, R. F. de
700 1 $aBALLOU, M. A.
700 1 $aSTELLA, T. R.
700 1 $aLEME, P. R.
773 $tPesquisa agropecuária Brasileira, Brasília, DF$gv. 46, n. 9, p. 1105-1111, set. 2011
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