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| 152. |  | GUIMARÃES, S. L.; BALDANI, J. I.; BALDANI, V. L. D.; JACOB-NETO, J. Adição de molibdênio ao inoculante turfoso com bactérias diazotróficas usado em duas cultivares de arroz irrigado. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 42, n. 3, p. 393-398, mar. 2007. Parceria: UFRRJ| Biblioteca(s): Embrapa Agrobiologia; Embrapa Unidades Centrais. |
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| 155. | | CAMELO, A.; GALVÃO, P. G.; VIDAL, M. S.; BALDANI, J. I. Análise do perfil de expressão dos genes gumD, cpaB, eglA e luxI em Gluconacetobacter diazotrophicus PAL5T colonizando plantas de cana-de-açúcar por qRT-PCR In: SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 13., 2013, Seropédica Ciência, saúde e esporte: caderno de resumos... Seropédica: Embrapa Agrobiologia, 2013.| Biblioteca(s): Embrapa Agrobiologia. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
24/10/2003 |
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Data da última atualização: |
24/10/2003 |
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Autoria: |
MARIN, V. A.; TEIXEIRA, K. R. S. dos; BALDANI, J. I. |
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Título: |
Characterization of amplified polymerase chain reaction glnB and nifH gene fragments of nitrogen-fixing Burkholderia species. |
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Ano de publicação: |
2003 |
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Fonte/Imprenta: |
Letters in Applied Microbiology, Oxford, v. 36, p. 77-82, 2003. |
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Idioma: |
Inglês |
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Conteúdo: |
Aims: To clone and sequence polymerase chain reaction (PCR)-amplified glnB and nifH genes of the nitrogen- fixing bacteria Burkholderia brasilensis strain M130, B. tropicalis strain PPe8 and B. kururiensis strain KP23. Methods and Resu1ts: The glnB and nifH gene fragments were amplified by PCR using universal degenerated primers. A very high percentage of similarity for the nifH (100%) and glnB (96%) genes was observed between strains M130 and KP23. A similarity of 100% for the nifH gene was also observed between strains M130 and PPe8. However, the identity for the glnB gene was 98% and the similarity 88%. The phylogenetic tree of the nifH gene showed a very high degree of similarity to the 16S rDNA gene. Conc1usions: The nitrogen-fixing bacteria of the Burkholderia genus formed a cluster separated from the other species of the genus mainly when the nifH rather than the glnB gene was used to construct the phylogenetic tree. Significance and Impact of the Study: Knowledge of the nifH and glnB gene sequences of B. brasilensis, B. tropicalis and B. kururiensis will support new studies on the diversity of these diazotrophs in natural environments.
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Palavras-Chave: |
BNF; FBN; Fixação biológica de nitrogênio; Nitrogen fixation biology. |
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Thesagro: |
Bactéria. |
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Thesaurus NAL: |
Burkholderia. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01831naa a2200217 a 4500
001 1624610
005 2003-10-24
008 2003 bl --- 0-- u #d
100 1 $aMARIN, V. A.
245 $aCharacterization of amplified polymerase chain reaction glnB and nifH gene fragments of nitrogen-fixing Burkholderia species.
260 $c2003
520 $aAims: To clone and sequence polymerase chain reaction (PCR)-amplified glnB and nifH genes of the nitrogen- fixing bacteria Burkholderia brasilensis strain M130, B. tropicalis strain PPe8 and B. kururiensis strain KP23. Methods and Resu1ts: The glnB and nifH gene fragments were amplified by PCR using universal degenerated primers. A very high percentage of similarity for the nifH (100%) and glnB (96%) genes was observed between strains M130 and KP23. A similarity of 100% for the nifH gene was also observed between strains M130 and PPe8. However, the identity for the glnB gene was 98% and the similarity 88%. The phylogenetic tree of the nifH gene showed a very high degree of similarity to the 16S rDNA gene. Conc1usions: The nitrogen-fixing bacteria of the Burkholderia genus formed a cluster separated from the other species of the genus mainly when the nifH rather than the glnB gene was used to construct the phylogenetic tree. Significance and Impact of the Study: Knowledge of the nifH and glnB gene sequences of B. brasilensis, B. tropicalis and B. kururiensis will support new studies on the diversity of these diazotrophs in natural environments.
650 $aBurkholderia
650 $aBactéria
653 $aBNF
653 $aFBN
653 $aFixação biológica de nitrogênio
653 $aNitrogen fixation biology
700 1 $aTEIXEIRA, K. R. S. dos
700 1 $aBALDANI, J. I.
773 $tLetters in Applied Microbiology, Oxford$gv. 36, p. 77-82, 2003.
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