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| 42. |  | PEREIRA-CARVALHO, R. C.; FONSECA, M. E. N.; NOGUEIRA, L.; FERNANDES-ACIOLI, N. A. N.; BOITEUX, L. S. Inheritance of a tolerant reaction to Tomato chlorosis crinivirus in tomato. Tropical Plant Pathology, Brasília, DF, v. 36, p. 1413, 2011. 1 CD-ROM. Suplemento. Edição dos resumos do 44º Congresso Brasileiro de Fitopatologia, 2011, Bento Gonçalves. Resumo 1555.| Biblioteca(s): Embrapa Hortaliças. |
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| 43. | | NOGUEIRA, I.; FONSECA, M. E. de N.; RESENDE, R. O.; BLAWID, R.; INOUE=NAGATA, A. K.; PEREIRA-CARVALHO, R. C. Diversidade de endossimbiontes em Bemisia tabaci coletadadas no tomateiro e diferentes hospedeiras em localidades do Centro Oeste. Tropical Plant Pathology, Brasília, DF, v. 37, 2012. 1 CD-ROM. Suplemento. Edição do 45º Congresso Brasileiro de Fitopatologia, 2012, Manaus. Resumo 805.| Biblioteca(s): Embrapa Hortaliças. |
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| 45. | | DOMINGOS, D. J.; CAVENAGHI, T. M. C. M.; GONTIJO, T. A.; DRUMOND, M. A.; CARVALHO, R. C. F. de. Composicao em especies, densidade e aspectos biologicos da fauna de termitas de cerrado em Sete Lagoas, MG. Ciencia e Cultura, v.38, n.1, p.199-207, 1986.| Biblioteca(s): Embrapa Cerrados. |
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| 46. | | IEDE, E. T.; LÁZZARI, S. M. N.; PENTEADO, S. R. C.; ZONTA-DE-CARVALHO, R. C.; RODRIGUES-TRENTINI, R. F. Ocorrência de Cinara pinivora (HOMOPTERA:. APHIDIDADAE, LACHNINAE) em reflorestamentos de Pinus spp. no Sul do Brasil. In: CONGRESSO BRASILEIRO DE ZOOLOGIA, 22., 1998, Recife, PE. Resumos. Recife: Universidade Federal de Pernambuco: Sociedade Brasileira de Zoologia, 1998.| Biblioteca(s): Embrapa Florestas. |
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| 49. | | FONSECA-BOITEUX, M. E. de N.; BOITEUX, L. S.; ABREU, H.; NOGUEIRA, I.; PEREIRA-CARVALHO, R. C. Physalis angulata: a new natural host of tomato chlorosis virus in Brasil. Plant Disease, Saint Paul, v. 97, n. 5, p. 692, May 2013. Disease notes| Biblioteca(s): Embrapa Hortaliças. |
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| 50. | | MACHADO, M. R.; CARVALHO, R. C. P.; MENDONZA, L. L.; FONSECA, M. E. N.; RIBEIRO, S. G.; BOITEUX, L. S. Phenotypic expression and spectrum of efficiency to four bipartite Begomovirus species of novel tomato resistance sources. In: SOLANACEAE CONFERENCE, 11., 2014, Porto Seguro. Book of abstracts. [s.l.]: [s.n.], 2014. p. 181 Posters 68.| Biblioteca(s): Embrapa Hortaliças. |
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| 51. | | PEREIRA-CARVALHO, R. C.; DIAZ-PENDÓN, J. A.; FERNANDÉZ-MUÑOZ, R.; BOITEUX, L. S.; MORIONES, E.; RESENDE, R. O. Estudo do mecanismo de resistência a begomovirus na linhagem de tomate 'TX-468' e seu efeito na dispersão do Tomato yellow leaf curl virus por Bemisia tabaci. Tropical Plant Pathology, Brasília, DF, v. 33, p. S294, ago. 2008. Suplemento. Resumo VIR 039. Trabalho apresentado no 41. Congresso Brasileiro de Fitopatologia, 41. Annual Meeting of the Brazilian Phytopathological Society, Belo Horizonte, 2008.| Biblioteca(s): Embrapa Hortaliças. |
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| 55. | | FERNANDES-ACIOLI, N. A. N.; PEREIRA-CARVALHO, R. C.; FONTENELE, R. S.; LACORTE, C.; RIBEIRO, S. G.; FONSECA, M. E. N.; BOITEUX, L. S. First report of Sida micrantha mosaic virus in Phaseolus vulgaris in Brazil. Plant disease, Saint Paul, v. 95, n. 9, p. 1196, Sept. 2011.| Biblioteca(s): Embrapa Hortaliças; Embrapa Recursos Genéticos e Biotecnologia. |
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| 56. | | BATISTA, J. G.; BOITEUX, L. S.; MELO, F. F. S.; MIRANDA, B. E. C.; RIBEIRO, S. da G.; FONSECA, M. E. N.; PEREIRA-CARVALHO, R. C. First report of maize striate mosaic virus (Geminiviridae) in sugarcane (Saccharum officinarum). Australasian Plant Disease Notes, v. 16, article 16, 2021. Na publicação: S. G. Ribeiro.| Biblioteca(s): Embrapa Hortaliças; Embrapa Recursos Genéticos e Biotecnologia. |
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| 57. | | FERNANDES-ACIOLI, N. A. N.; FONSECA, M. E. N.; PEREIRA-CARVALHO, R. C.; FONTENELE, R. S.; LACORTE, C.; RIBEIRO, S. G.; BOITEUX, L. S. Sequência completa do DNA-A de uma nova espécie de Begomovirus infectando tomateiro no Estado de Tocantins, Brasil. Tropical Plant Pathology, Brasília, DF, v. 36, p. 1402, 2011. 1 CD-ROM. Resumo 1381. Suplemento. Edição dos resumos do 44º Congresso Brasileiro de Fitopatologia, 2011, Bento Gonçalves.| Biblioteca(s): Embrapa Hortaliças. |
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| 58. | | KNUPPEL, C. A.; CARVALHO, R. C. da S.; CASTANHEIRA, D. T.; BATISTA, F. K. O.; GUEDES, J. M.; GUIMARÃES, R. J.; CARVALHO, M. A. de F. Taxa fotossintética e condutância estomática de cafeeiros cultivados com diferentes tipos de manejo. In: CONGRESSO BRASILEIRO DE PESQUISAS CAFEEIRAS, 43., 2017, Poços de Caldas. Novas tecnologias para um bom café produzir. Brasília, DF: Embrapa Café, 2017.| Biblioteca(s): Embrapa Café. |
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| 59. | | DUARTE, M. F.; FONSECA, M. E. N.; BOITEUX, L. S.; COSTA, H.; RIBEIRO, B. M.; MELO, F. L.; PEREIRA-CARVALHO, R. C. Identification of Physalis angulata (Solanaceae) as a Natural Alternative Weed Host of Tomato Severe Rugose Virus in Brazil Plant Disease, v. 104, n. 2, p. 600, Feb. 2020.| Biblioteca(s): Embrapa Hortaliças. |
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| 60. | | PEREIRA-CARVALHO, R. C.; BOITEUX, L. S.; FONSECA, M. E. N.; MORIONES, E.; FERNANDEZ-MUÑOZ, R.; DIAZ-PENDÓN, J.A.; CHARCHAR, J. M.; RESENDE, R. O. Identification in solanum (section lycopersicon) species of genetic sources combining resistance to root-knot nematodes, monopartite and bipartite begomovirus. Virus Reviews & Research, São Paulo, v. 14, Supl. 1, p. 247-248, nov. 2009. Suplemento 1. Resumo.Trabalho apresentado no 20. National Meeting of Virology, 2009, Brasília, Distrito Federal, Brasil.| Biblioteca(s): Embrapa Hortaliças. |
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| Registros recuperados : 112 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
20/09/2021 |
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Data da última atualização: |
28/08/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
RAMOS, E. T. de A.; MENESES, C. H. S. G.; VIDAL, M. S.; BALDANI, J. I. |
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Afiliação: |
ELIZABETH TEIXEIRA DE ALMEIDA RAMOS, UFRRJ; CARLOS HENRIQUE SALVINO GADELHA MENESES, UEPB; MARCIA SOARES VIDAL, CNPAB; JOSE IVO BALDANI, CNPAB. |
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Título: |
Characterisation and action mode of Gluconacin, a bacteriocin with antimicrobial activity against Xanthomonas albilineans. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Annals of Applied Biology, Annals of Applied Biology, v. 180, p. 163-175. 2022. |
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DOI: |
DOI: 10.1111/aab.12721 |
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Idioma: |
Inglês |
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Conteúdo: |
Gluconacin, a bacteriocin produced by Gluconacetobacter diazotrophicus PAL5, has previously shown a large spectrum of inhibitory activity against beneficial and phytopatho- genic bacteria. The present study proposes a three-dimensional (3D) structure for Gluconacin based on modelling and describes some physicochemical characteristics and the effect of the peptide on cells of the phytopathogenic bacterium Xanthomonas albilineans ICMP196. The Gluconacin 3D structural model results demonstrated the 7 ?helices and 11 ?-sheets. Growth inhibition of the indicator microorganism, X. albilineans, occurred in the first moments of contact with Gluconacin (0.25 ?g ?l 1 ). Treatment of bacterial cells caused significant loss of inorganic phosphate and UV-absorbing materials. Scanning electron microscopy images showed that Gluconacin-treated X. lbilineans cells were fully lysed. These results suggest that the mode of action of this peptide involves altered membrane integrity and increased permeability, resulting in complete cell lysis. Physicochemical characterisation demonstrated stability of biological activity under high temperatures, including autoclaving conditions and low pH. The activity was reduced after treatment with proteases and some surfactants and abolished with ethylenediaminetetraacetic acid (EDTA). The 3D conformation model indicates the possibility of a hydrophobic region, which could possibly interact directly with the cell membrane of target bacteria. In conclusion, the ability of Gluconacin to inhibit growth of phytopathogens of agricultural importance opens up new opportunities for biotechnological applications and, consequently, a reduction in the use of pesticides. MenosGluconacin, a bacteriocin produced by Gluconacetobacter diazotrophicus PAL5, has previously shown a large spectrum of inhibitory activity against beneficial and phytopatho- genic bacteria. The present study proposes a three-dimensional (3D) structure for Gluconacin based on modelling and describes some physicochemical characteristics and the effect of the peptide on cells of the phytopathogenic bacterium Xanthomonas albilineans ICMP196. The Gluconacin 3D structural model results demonstrated the 7 ?helices and 11 ?-sheets. Growth inhibition of the indicator microorganism, X. albilineans, occurred in the first moments of contact with Gluconacin (0.25 ?g ?l 1 ). Treatment of bacterial cells caused significant loss of inorganic phosphate and UV-absorbing materials. Scanning electron microscopy images showed that Gluconacin-treated X. lbilineans cells were fully lysed. These results suggest that the mode of action of this peptide involves altered membrane integrity and increased permeability, resulting in complete cell lysis. Physicochemical characterisation demonstrated stability of biological activity under high temperatures, including autoclaving conditions and low pH. The activity was reduced after treatment with proteases and some surfactants and abolished with ethylenediaminetetraacetic acid (EDTA). The 3D conformation model indicates the possibility of a hydrophobic region, which could possibly interact directly with the cell membrane of target bacteria. In conclusion, th... Mostrar Tudo |
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Palavras-Chave: |
Antimicrobial peptide; Bactericidal effect. |
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Thesaurus NAL: |
Biological control; Gluconacetobacter diazotrophicus. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 02455naa a2200217 a 4500
001 2134566
005 2023-08-28
008 2022 bl uuuu u00u1 u #d
024 7 $aDOI: 10.1111/aab.12721$2DOI
100 1 $aRAMOS, E. T. de A.
245 $aCharacterisation and action mode of Gluconacin, a bacteriocin with antimicrobial activity against Xanthomonas albilineans.$h[electronic resource]
260 $c2022
520 $aGluconacin, a bacteriocin produced by Gluconacetobacter diazotrophicus PAL5, has previously shown a large spectrum of inhibitory activity against beneficial and phytopatho- genic bacteria. The present study proposes a three-dimensional (3D) structure for Gluconacin based on modelling and describes some physicochemical characteristics and the effect of the peptide on cells of the phytopathogenic bacterium Xanthomonas albilineans ICMP196. The Gluconacin 3D structural model results demonstrated the 7 ?helices and 11 ?-sheets. Growth inhibition of the indicator microorganism, X. albilineans, occurred in the first moments of contact with Gluconacin (0.25 ?g ?l 1 ). Treatment of bacterial cells caused significant loss of inorganic phosphate and UV-absorbing materials. Scanning electron microscopy images showed that Gluconacin-treated X. lbilineans cells were fully lysed. These results suggest that the mode of action of this peptide involves altered membrane integrity and increased permeability, resulting in complete cell lysis. Physicochemical characterisation demonstrated stability of biological activity under high temperatures, including autoclaving conditions and low pH. The activity was reduced after treatment with proteases and some surfactants and abolished with ethylenediaminetetraacetic acid (EDTA). The 3D conformation model indicates the possibility of a hydrophobic region, which could possibly interact directly with the cell membrane of target bacteria. In conclusion, the ability of Gluconacin to inhibit growth of phytopathogens of agricultural importance opens up new opportunities for biotechnological applications and, consequently, a reduction in the use of pesticides.
650 $aBiological control
650 $aGluconacetobacter diazotrophicus
653 $aAntimicrobial peptide
653 $aBactericidal effect
700 1 $aMENESES, C. H. S. G.
700 1 $aVIDAL, M. S.
700 1 $aBALDANI, J. I.
773 $tAnnals of Applied Biology, Annals of Applied Biology$gv. 180, p. 163-175. 2022.
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