Data/hora: 22/06/2024 08:44:40

Biblioteca(s): | Embrapa Unidades Centrais.
Data corrente: | 10/03/2016
Data da última atualização: | 10/03/2016
Autoria: | CAGNINI, D. Q.; CUNHA, P. H. J.; PANTOJA, J. C. F.; BADIAL, P. R.; OLIVEIRA-FILHO, J. P. de; ARAÚJO-JUNIOR, J. P.; ALFIERI, A. A.; BORGES, A. S.
Afiliação: | DIDIER Q. CAGNINI, FMVZ/UNESP; PAULO H. J. CUNHA, UFG; JOSÉ C. F. PANTOJA, FMVZ/UNESP; PERES R. BADIAL, FMVZ/UNESP; JOSÉ PAES DE OLIVEIRA-FILHO, FMVZ/UNESP; JOÃO P. ARAÚJO-JUNIOR, UNESP; AMAURI A. ALFIERI, UNESP; ALEXANDRE S. BORGES, FMVZ/UNESP.
Título: | Histopathological, immunohistochemical, and molecular study of BHV-5 infection in the central nervous system of experimentally infected calves.
Ano de publicação: | 2015
Fonte/Imprenta: | Pesquisa Veterinária Brasileira, Brasília, DF, v. 35. n. 4, p. 337-343, abr. 2015.
Idioma: | Inglês
Conteúdo: | Bovine meningoencephalitis caused by BHV-5, a double-stranded DNA enveloped virus that belongs to the family Herpesviridae and subfamily Alphaherpesvirinae, is an important differential diagnosis of central nervous diseases. The aim of this study was to describe the histological changes in the central nervous system of calves experimentally infected with BHV-5 and compare these changes with the PCR and IHC results. Formalin-fixed paraffin--embedded central nervous system samples from calves previously inoculated with BHV-5 were microscopically evaluated and tested using IHC and PCR. All the animals presented with nonsuppurative meningoencephalitis. From 18 evaluated areas of each calf, 32.41% and 35.19% were positive by IHC and PCR, respectively. The telencephalon presented more accentuated lesions and positive areas in the PCR than other encephalic areas and was the best sampling area for diagnostic purposes. Positive areas in the IHC and PCR were more injured than IHC and PCR negative areas. The animal with neurological signs showed more PCR- and IHC-positive areas than the other animals.
Palavras-Chave: | Disease of cattle; Herpesvirus bovino 5; Meningoencefalite; Neuropatologia.
Thesagro: | Bovino; Doença.
Thesaurus Nal: | Bovine herpesvirus 5; Meningoencephalitis; Neuropathology.
Categoria do assunto: | --
URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/140970/1/Histopathological-immunohistochemical.pdf
Registro original: | Embrapa Unidades Centrais (AI-SEDE)
Exemplares: | AI-SEDE - (59095-1) - UPE - AP - PP - 636.08905 - P474 -  - DIS